Restriction map for
pF1KB9492
(Linear) (Six-base) MAPSORT of: KIBB9492.seq Check: 2966 from: 1 to: 3609
KIBB9492.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AccI GT'mk_AC
Cuts at: 0 1461 3609
Size: 1461 2148
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 408 1176 1472 3502 3609
Size: 408 768 296 2030 107
Fragments arranged by size:
2030 768 408 296 107
AflII C'TTAA_G
Cuts at: 0 157 3609
Size: 157 3452
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3203 3235 3609
Size: 3203 32 374
Fragments arranged by size:
3203 374 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3480 3591 3609
Size: 3480 111 18
Fragments arranged by size:
3480 111 18
ApaLI G'TGCA_C
Cuts at: 0 2024 3609
Size: 2024 1585
ApoI r'AATT_y
Cuts at: 0 258 668 1420 1545 1660 1693 2720 3609
Size: 258 410 752 125 115 33 1027 889
Fragments arranged by size:
1027 889 752 410 258 125 115 33
AseI AT'TA_AT
Cuts at: 0 847 3609
Size: 847 2762
BamHI G'GATC_C
Cuts at: 0 1035 3609
Size: 1035 2574
BanII G_rGCy'C
Cuts at: 0 156 1584 3609
Size: 156 1428 2025
Fragments arranged by size:
2025 1428 156
BbsI GAAGACnn'nnnn_
Cuts at: 0 827 1273 1973 2531 2863 3609
Size: 827 446 700 558 332 746
Fragments arranged by size:
827 746 700 558 446 332
BbvCI CC'TCA_GC
Cuts at: 0 2168 3609
Size: 2168 1441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2880 2914 3609
Size: 2880 34 695
Fragments arranged by size:
2880 695 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1814 3609
Size: 1814 1795
BfrBI ATG'CAT
Cuts at: 0 875 1926 3083 3609
Size: 875 1051 1157 526
Fragments arranged by size:
1157 1051 875 526
BglII A'GATC_T
Cuts at: 0 3006 3609
Size: 3006 603
BlpI GC'TnA_GC
Cuts at: 0 491 3144 3609
Size: 491 2653 465
Fragments arranged by size:
2653 491 465
Bme1580I G_kGCm'C
Cuts at: 0 2028 2742 3609
Size: 2028 714 867
Fragments arranged by size:
2028 867 714
BmrI ACTGGGnnnn_n'
Cuts at: 0 557 3609
Size: 557 3052
BmtI G_CTAG'C
Cuts at: 0 2128 3609
Size: 2128 1481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 149 2869 3609
Size: 70 79 2720 740
Fragments arranged by size:
2720 740 79 70
Bpu10I CC'TnA_GC
Cuts at: 0 2168 3609
Size: 2168 1441
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1537 2207 3133 3609
Size: 1537 670 926 476
Fragments arranged by size:
1537 926 670 476
BsaI GGTCTCn'nnnn_
Cuts at: 0 1780 3609
Size: 1780 1829
BsaBI GATnn'nnATC
Cuts at: 0 2396 3609
Size: 2396 1213
BsaWI w'CCGG_w
Cuts at: 0 2780 3609
Size: 2780 829
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 383 413 569 599 3203 3233 3609
Size: 383 30 156 30 2604 30 376
Fragments arranged by size:
2604 383 376 156 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 122 125 3125 3128 3208 3609
Size: 122 3 3000 3 80 401
Fragments arranged by size:
3000 401 122 80 3 3
BseYI C'CCAG_C
Cuts at: 0 54 681 3341 3609
Size: 54 627 2660 268
Fragments arranged by size:
2660 627 268 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2708 2825 3609
Size: 2708 117 784
Fragments arranged by size:
2708 784 117
BsiHKAI G_wGCw'C
Cuts at: 0 2028 3609
Size: 2028 1581
BsmI GAATG_Cn'
Cuts at: 0 227 450 2931 3609
Size: 227 223 2481 678
Fragments arranged by size:
2481 678 227 223
Bsp1286I G_dGCh'C
Cuts at: 0 156 1584 2028 2742 3609
Size: 156 1428 444 714 867
Fragments arranged by size:
1428 867 714 444 156
BspEI T'CCGG_A
Cuts at: 0 2780 3609
Size: 2780 829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3296 3432 3609
Size: 3296 136 177
Fragments arranged by size:
3296 177 136
BsrDI GCAATG_nn'
Cuts at: 0 1370 1380 2135 3609
Size: 1370 10 755 1474
Fragments arranged by size:
1474 1370 755 10
BsrGI T'GTAC_A
Cuts at: 0 299 1974 3609
Size: 299 1675 1635
Fragments arranged by size:
1675 1635 299
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1501 3609
Size: 1501 2108
BstXI CCAn_nnnn'nTGG
Cuts at: 0 620 3379 3609
Size: 620 2759 230
Fragments arranged by size:
2759 620 230
BstYI r'GATC_y
Cuts at: 0 1006 1035 3006 3609
Size: 1006 29 1971 603
Fragments arranged by size:
1971 1006 603 29
BtgI C'CryG_G
Cuts at: 0 1098 2609 3609
Size: 1098 1511 1000
Fragments arranged by size:
1511 1098 1000
BtsI GCAGTG_nn'
Cuts at: 0 357 391 976 3609
Size: 357 34 585 2633
Fragments arranged by size:
2633 585 357 34
DraI TTT'AAA
Cuts at: 0 1215 3233 3609
Size: 1215 2018 376
Fragments arranged by size:
2018 1215 376
EaeI y'GGCC_r
Cuts at: 0 1907 3275 3609
Size: 1907 1368 334
Fragments arranged by size:
1907 1368 334
EarI CTCTTCn'nnn_
Cuts at: 0 1363 2050 2399 3181 3609
Size: 1363 687 349 782 428
Fragments arranged by size:
1363 782 687 428 349
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3047 3609
Size: 3047 562
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 149 408 1176 1472 2869 3502 3609
Size: 70 79 259 768 296 1397 633 107
Fragments arranged by size:
1397 768 633 296 259 107 79 70
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1214 3609
Size: 1214 2395
EcoO109I rG'GnC_Cy
Cuts at: 0 3125 3504 3609
Size: 3125 379 105
Fragments arranged by size:
3125 379 105
EcoRI G'AATT_C
Cuts at: 0 258 1693 2720 3609
Size: 258 1435 1027 889
Fragments arranged by size:
1435 1027 889 258
EcoRV GAT'ATC
Cuts at: 0 2211 3609
Size: 2211 1398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1260 1713 2891 3609
Size: 1260 453 1178 718
Fragments arranged by size:
1260 1178 718 453
HaeII r_GCGC'y
Cuts at: 0 2004 3609
Size: 2004 1605
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 159 191 1050 1082 1721 1753 1911 1943
Size: 159 32 859 32 639 32 158 32
Cuts at: 1943 3609
Size: 1666
Fragments arranged by size:
1666 859 639 159 158 32 32 32
32
HincII GTy'rAC
Cuts at: 0 99 3609
Size: 99 3510
HindIII A'AGCT_T
Cuts at: 0 604 3609
Size: 604 3005
MfeI C'AATT_G
Cuts at: 0 1933 2941 3609
Size: 1933 1008 668
Fragments arranged by size:
1933 1008 668
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3195 3609
Size: 3195 414
MscI TGG'CCA
Cuts at: 0 1909 3277 3609
Size: 1909 1368 332
Fragments arranged by size:
1909 1368 332
MslI CAynn'nnrTG
Cuts at: 0 473 618 870 3609
Size: 473 145 252 2739
Fragments arranged by size:
2739 473 252 145
MspA1I CmG'CkG
Cuts at: 0 149 1498 2107 2700 3345 3609
Size: 149 1349 609 593 645 264
Fragments arranged by size:
1349 645 609 593 264 149
NcoI C'CATG_G
Cuts at: 0 1098 2609 3609
Size: 1098 1511 1000
Fragments arranged by size:
1511 1098 1000
NdeI CA'TA_TG
Cuts at: 0 2997 3609
Size: 2997 612
NheI G'CTAG_C
Cuts at: 0 2124 3609
Size: 2124 1485
NsiI A_TGCA'T
Cuts at: 0 877 1928 3085 3609
Size: 877 1051 1157 524
Fragments arranged by size:
1157 1051 877 524
NspI r_CATG'y
Cuts at: 0 681 1859 2139 2812 2895 3609
Size: 681 1178 280 673 83 714
Fragments arranged by size:
1178 714 681 673 280 83
PfoI T'CCnGG_A
Cuts at: 0 1676 3371 3609
Size: 1676 1695 238
Fragments arranged by size:
1695 1676 238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3203 3235 3609
Size: 3203 32 374
Fragments arranged by size:
3203 374 32
PpuMI rG'GwC_Cy
Cuts at: 0 3125 3609
Size: 3125 484
PshAI GACnn'nnGTC
Cuts at: 0 860 3609
Size: 860 2749
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3277 3309 3609
Size: 3277 32 300
Fragments arranged by size:
3277 300 32
PstI C_TGCA'G
Cuts at: 0 148 2016 2112 2699 3609
Size: 148 1868 96 587 910
Fragments arranged by size:
1868 910 587 148 96
PvuII CAG'CTG
Cuts at: 0 1498 2107 2700 3345 3609
Size: 1498 609 593 645 264
Fragments arranged by size:
1498 645 609 593 264
SapI GCTCTTCn'nnn_
Cuts at: 0 1363 3609
Size: 1363 2246
SexAI A'CCwGG_T
Cuts at: 0 3398 3609
Size: 3398 211
SfcI C'TryA_G
Cuts at: 0 144 1462 2012 2108 2695 3537 3609
Size: 144 1318 550 96 587 842 72
Fragments arranged by size:
1318 842 587 550 144 96 72
SmlI C'TyrA_G
Cuts at: 0 157 1552 2222 3112 3609
Size: 157 1395 670 890 497
Fragments arranged by size:
1395 890 670 497 157
SspI AAT'ATT
Cuts at: 0 221 375 2509 3609
Size: 221 154 2134 1100
Fragments arranged by size:
2134 1100 221 154
StuI AGG'CCT
Cuts at: 0 1220 3609
Size: 1220 2389
StyI C'CwwG_G
Cuts at: 0 212 1098 1387 2609 2624 2634 3507 3558
Size: 212 886 289 1222 15 10 873 51
Cuts at: 3558 3609
Size: 51
Fragments arranged by size:
1222 886 873 289 212 51 51 15
10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 673 1013 3609
Size: 673 340 2596
Fragments arranged by size:
2596 673 340
TatI w'GTAC_w
Cuts at: 0 299 390 438 1974 2423 3609
Size: 299 91 48 1536 449 1186
Fragments arranged by size:
1536 1186 449 299 91 48
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2322 2354 2711 2743 3609
Size: 2322 32 357 32 866
Fragments arranged by size:
2322 866 357 32 32
XbaI T'CTAG_A
Cuts at: 0 265 909 3609
Size: 265 644 2700
Fragments arranged by size:
2700 644 265
XmnI GAAnn'nnTTC
Cuts at: 0 275 1413 3609
Size: 275 1138 2196
Fragments arranged by size:
2196 1138 275
Enzymes that do cut:
AccI AcuI AflII AloI AlwNI ApaLI ApoI AseI
BamHI BanII BbsI BbvCI BcgI BciVI BfrBI BglII
BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrDI BsrGI BstAPI BstXI BstYI
BtgI BtsI DraI EaeI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII
MfeI MmeI MscI MslI MspA1I NcoI NdeI NheI
NsiI NspI PfoI PpiI PpuMI PshAI PsrI PstI
PvuII SapI SexAI SfcI SmlI SspI StuI StyI
TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI ApaI AscI AsiSI AvaI AvrII BaeI
BanI BbeI BclI BglI BmgBI BplI BsaAI BsaHI
BsiEI BsiWI BsmBI BspHI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII
DrdI EagI EcoICRI FseI FspI FspAI HpaI KasI
KpnI MluI NaeI NarI NgoMIV NotI NruI PacI
PasI PciI PflMI PmeI PmlI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI Tth111I XcmI XhoI XmaI ZraI