Restriction map for
pF1KB9491
(Linear) (Six-base) MAPSORT of: KIBB9491.seq Check: 4789 from: 1 to: 3420
KIBB9491.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AclI AA'CG_TT
Cuts at: 0 1110 3420
Size: 1110 2310
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1112 1711 1732 2392 3420
Size: 961 151 599 21 660 1028
Fragments arranged by size:
1028 961 660 599 151 21
AflIII A'CryG_T
Cuts at: 0 2250 3420
Size: 2250 1170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 215 440 3420
Size: 215 225 2980
Fragments arranged by size:
2980 225 215
AlwNI CAG_nnn'CTG
Cuts at: 0 2109 3420
Size: 2109 1311
ApaI G_GGCC'C
Cuts at: 0 3326 3420
Size: 3326 94
ApoI r'AATT_y
Cuts at: 0 55 236 245 382 490 868 2713 2729
Size: 55 181 9 137 108 378 1845 16
Cuts at: 2729 3420
Size: 691
Fragments arranged by size:
1845 691 378 181 137 108 55 16
9
AseI AT'TA_AT
Cuts at: 0 3122 3420
Size: 3122 298
AvaI C'yCGr_G
Cuts at: 0 9 3420
Size: 9 3411
BamHI G'GATC_C
Cuts at: 0 3243 3420
Size: 3243 177
BanII G_rGCy'C
Cuts at: 0 2304 3326 3420
Size: 2304 1022 94
Fragments arranged by size:
2304 1022 94
BbsI GAAGACnn'nnnn_
Cuts at: 0 569 946 1631 1717 2583 3420
Size: 569 377 685 86 866 837
Fragments arranged by size:
866 837 685 569 377 86
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 123 157 3420
Size: 123 34 3263
Fragments arranged by size:
3263 123 34
BglII A'GATC_T
Cuts at: 0 1131 2073 2610 3420
Size: 1131 942 537 810
Fragments arranged by size:
1131 942 810 537
BlpI GC'TnA_GC
Cuts at: 0 3134 3420
Size: 3134 286
Bme1580I G_kGCm'C
Cuts at: 0 95 2697 3326 3420
Size: 95 2602 629 94
Fragments arranged by size:
2602 629 95 94
BmrI ACTGGGnnnn_n'
Cuts at: 0 2121 2154 3420
Size: 2121 33 1266
Fragments arranged by size:
2121 1266 33
BmtI G_CTAG'C
Cuts at: 0 1096 3420
Size: 1096 2324
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1259 3362 3420
Size: 1259 2103 58
Fragments arranged by size:
2103 1259 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1345 2131 3245 3347 3359 3420
Size: 1345 786 1114 102 12 61
Fragments arranged by size:
1345 1114 786 102 61 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 2869 3084 3420
Size: 721 2148 215 336
Fragments arranged by size:
2148 721 336 215
BsaI GGTCTCn'nnnn_
Cuts at: 0 1780 3002 3420
Size: 1780 1222 418
Fragments arranged by size:
1780 1222 418
BsaBI GATnn'nnATC
Cuts at: 0 1580 3420
Size: 1580 1840
BsaWI w'CCGG_w
Cuts at: 0 2384 3420
Size: 2384 1036
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 667 697 3420
Size: 667 30 2723
Fragments arranged by size:
2723 667 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 313 718 1423 2224 3420
Size: 313 405 705 801 1196
Fragments arranged by size:
1196 801 705 405 313
BseYI C'CCAG_C
Cuts at: 0 88 146 434 3383 3420
Size: 88 58 288 2949 37
Fragments arranged by size:
2949 288 88 58 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 3420
Size: 446 2974
BsiHKAI G_wGCw'C
Cuts at: 0 2304 2594 3201 3420
Size: 2304 290 607 219
Fragments arranged by size:
2304 607 290 219
BsmI GAATG_Cn'
Cuts at: 0 1166 2328 3420
Size: 1166 1162 1092
Fragments arranged by size:
1166 1162 1092
Bsp1286I G_dGCh'C
Cuts at: 0 95 2304 2594 2697 3201 3326 3420
Size: 95 2209 290 103 504 125 94
Fragments arranged by size:
2209 504 290 125 103 95 94
BspEI T'CCGG_A
Cuts at: 0 2384 3420
Size: 2384 1036
BspHI T'CATG_A
Cuts at: 0 225 2679 2816 3420
Size: 225 2454 137 604
Fragments arranged by size:
2454 604 225 137
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1397 3420
Size: 1397 2023
BsrDI GCAATG_nn'
Cuts at: 0 2006 3228 3420
Size: 2006 1222 192
Fragments arranged by size:
2006 1222 192
BsrFI r'CCGG_y
Cuts at: 0 69 3420
Size: 69 3351
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2151 3420
Size: 2151 1269
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2709 3420
Size: 2709 711
BstYI r'GATC_y
Cuts at: 0 1131 2073 2610 3243 3378 3420
Size: 1131 942 537 633 135 42
Fragments arranged by size:
1131 942 633 537 135 42
DraI TTT'AAA
Cuts at: 0 173 2786 3420
Size: 173 2613 634
Fragments arranged by size:
2613 634 173
EaeI y'GGCC_r
Cuts at: 0 50 71 1656 3420
Size: 50 21 1585 1764
Fragments arranged by size:
1764 1585 50 21
EarI CTCTTCn'nnn_
Cuts at: 0 470 1247 1292 1400 1565 1868 1955 1976
Size: 470 777 45 108 165 303 87 21
Cuts at: 1976 2270 2420 3420
Size: 294 150 1000
Fragments arranged by size:
1000 777 470 303 294 165 150 108
87 45 21
EcoICRI GAG'CTC
Cuts at: 0 2302 3420
Size: 2302 1118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1112 1345 1711 1732 2131 2392 3245
Size: 961 151 233 366 21 399 261 853
Cuts at: 3245 3347 3359 3420
Size: 102 12 61
Fragments arranged by size:
961 853 399 366 261 233 151 102
61 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1570 3420
Size: 1570 1850
EcoO109I rG'GnC_Cy
Cuts at: 0 3322 3420
Size: 3322 98
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 874 3013 3420
Size: 874 2139 407
Fragments arranged by size:
2139 874 407
HaeII r_GCGC'y
Cuts at: 0 3390 3420
Size: 3390 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 667 699 1195 1225 1227 1257
Size: 181 32 454 32 496 30 2 30
Cuts at: 1257 1914 1946 1978 2010 2329 2361 2999 3031
Size: 657 32 32 32 319 32 638 32
Cuts at: 3031 3420
Size: 389
Fragments arranged by size:
657 638 496 454 389 319 181 32
32 32 32 32 32 32 30 30
2
HindIII A'AGCT_T
Cuts at: 0 389 973 1124 3356 3420
Size: 389 584 151 2232 64
Fragments arranged by size:
2232 584 389 151 64
MfeI C'AATT_G
Cuts at: 0 1478 1853 2495 3420
Size: 1478 375 642 925
Fragments arranged by size:
1478 925 642 375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 2789 3420
Size: 468 2321 631
Fragments arranged by size:
2321 631 468
MscI TGG'CCA
Cuts at: 0 1658 3420
Size: 1658 1762
MslI CAynn'nnrTG
Cuts at: 0 358 1496 2483 2678 3420
Size: 358 1138 987 195 742
Fragments arranged by size:
1138 987 742 358 195
MspA1I CmG'CkG
Cuts at: 0 84 1099 2181 2491 2536 3054 3420
Size: 84 1015 1082 310 45 518 366
Fragments arranged by size:
1082 1015 518 366 310 84 45
NaeI GCC'GGC
Cuts at: 0 71 3420
Size: 71 3349
NdeI CA'TA_TG
Cuts at: 0 1472 2596 3420
Size: 1472 1124 824
Fragments arranged by size:
1472 1124 824
NgoMIV G'CCGG_C
Cuts at: 0 69 3420
Size: 69 3351
NheI G'CTAG_C
Cuts at: 0 1092 3420
Size: 1092 2328
NspI r_CATG'y
Cuts at: 0 417 442 2254 2488 3420
Size: 417 25 1812 234 932
Fragments arranged by size:
1812 932 417 234 25
PciI A'CATG_T
Cuts at: 0 2250 3420
Size: 2250 1170
PflMI CCAn_nnn'nTGG
Cuts at: 0 1290 1671 3420
Size: 1290 381 1749
Fragments arranged by size:
1749 1290 381
PsiI TTA'TAA
Cuts at: 0 395 3420
Size: 395 3025
PspOMI G'GGCC_C
Cuts at: 0 3322 3420
Size: 3322 98
PstI C_TGCA'G
Cuts at: 0 1707 2337 3420
Size: 1707 630 1083
Fragments arranged by size:
1707 1083 630
PvuII CAG'CTG
Cuts at: 0 84 1099 2491 2536 3054 3420
Size: 84 1015 1392 45 518 366
Fragments arranged by size:
1392 1015 518 366 84 45
SacI G_AGCT'C
Cuts at: 0 2304 3420
Size: 2304 1116
SapI GCTCTTCn'nnn_
Cuts at: 0 1292 3420
Size: 1292 2128
ScaI AGT'ACT
Cuts at: 0 1619 3420
Size: 1619 1801
SfcI C'TryA_G
Cuts at: 0 105 829 902 1703 2333 3420
Size: 105 724 73 801 630 1087
Fragments arranged by size:
1087 801 724 630 105 73
SmaI CCC'GGG
Cuts at: 0 11 3420
Size: 11 3409
SmlI C'TyrA_G
Cuts at: 0 700 2884 3099 3420
Size: 700 2184 215 321
Fragments arranged by size:
2184 700 321 215
SspI AAT'ATT
Cuts at: 0 272 1366 3420
Size: 272 1094 2054
Fragments arranged by size:
2054 1094 272
StyI C'CwwG_G
Cuts at: 0 155 1040 1790 3420
Size: 155 885 750 1630
Fragments arranged by size:
1630 885 750 155
TatI w'GTAC_w
Cuts at: 0 528 1617 3420
Size: 528 1089 1803
Fragments arranged by size:
1803 1089 528
Tth111I GACn'n_nGTC
Cuts at: 0 459 3420
Size: 459 2961
XbaI T'CTAG_A
Cuts at: 0 463 1317 3420
Size: 463 854 2103
Fragments arranged by size:
2103 854 463
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 81 3420
Size: 81 3339
XmaI C'CCGG_G
Cuts at: 0 9 3420
Size: 9 3411
XmnI GAAnn'nnTTC
Cuts at: 0 236 1745 3420
Size: 236 1509 1675
Fragments arranged by size:
1675 1509 236
Enzymes that do cut:
AclI AcuI AflIII AlfI AlwNI ApaI ApoI AseI
AvaI BamHI BanII BbsI BcgI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BstAPI BstXI BstYI DraI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HindIII MfeI MmeI MscI MslI MspA1I NaeI
NdeI NgoMIV NheI NspI PciI PflMI PsiI PspOMI
PstI PvuII SacI SapI ScaI SfcI SmaI SmlI
SspI StyI TatI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AloI ApaLI AscI AsiSI AvrII BaeI BanI
BbeI BbvCI BciVI BclI BfrBI BglI BmgBI Bpu10I
BsaAI BsaHI BsiEI BsiWI BsmBI BsrBI BsrGI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EciI EcoRI EcoRV
FseI FspI FspAI HincII HpaI KasI KpnI MluI
NarI NcoI NotI NruI NsiI PacI PasI PfoI
PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI StuI SwaI
TaqII TstI XhoI ZraI