Restriction map for
pF1KB9490
(Linear) (Six-base) MAPSORT of: KIBB9490.seq Check: 4116 from: 1 to: 3564
KIBB9490.orf
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2357 3012 3564
Size: 2357 655 552
Fragments arranged by size:
2357 655 552
Acc65I G'GTAC_C
Cuts at: 0 128 3564
Size: 128 3436
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1735 2459 3218 3564
Size: 1735 724 759 346
Fragments arranged by size:
1735 759 724 346
AflII C'TTAA_G
Cuts at: 0 699 3564
Size: 699 2865
AflIII A'CryG_T
Cuts at: 0 28 840 3564
Size: 28 812 2724
Fragments arranged by size:
2724 812 28
AhdI GACnn_n'nnGTC
Cuts at: 0 2571 3564
Size: 2571 993
AleI CACnn'nnGTG
Cuts at: 0 3524 3564
Size: 3524 40
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1622 3564
Size: 1622 1942
AlwNI CAG_nnn'CTG
Cuts at: 0 2327 3564
Size: 2327 1237
ApoI r'AATT_y
Cuts at: 0 47 499 551 569 608 738 879 3094
Size: 47 452 52 18 39 130 141 2215
Cuts at: 3094 3187 3458 3564
Size: 93 271 106
Fragments arranged by size:
2215 452 271 141 130 106 93 52
47 39 18
AvaI C'yCGr_G
Cuts at: 0 1415 1993 2983 3564
Size: 1415 578 990 581
Fragments arranged by size:
1415 990 581 578
BamHI G'GATC_C
Cuts at: 0 977 3564
Size: 977 2587
BanI G'GyrC_C
Cuts at: 0 128 632 1307 3564
Size: 128 504 675 2257
Fragments arranged by size:
2257 675 504 128
BanII G_rGCy'C
Cuts at: 0 2548 3176 3291 3347 3564
Size: 2548 628 115 56 217
Fragments arranged by size:
2548 628 217 115 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 857 1640 1741 2465 2588 3224 3380 3564
Size: 857 783 101 724 123 636 156 184
Fragments arranged by size:
857 783 724 636 184 156 123 101
BbvCI CC'TCA_GC
Cuts at: 0 3322 3564
Size: 3322 242
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1480 1514 3564
Size: 1480 34 2050
Fragments arranged by size:
2050 1480 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 628 3564
Size: 628 2936
BglII A'GATC_T
Cuts at: 0 222 927 1581 2814 3564
Size: 222 705 654 1233 750
Fragments arranged by size:
1233 750 705 654 222
BlpI GC'TnA_GC
Cuts at: 0 771 3564
Size: 771 2793
BmgBI CAC'GTC
Cuts at: 0 2568 2674 3564
Size: 2568 106 890
Fragments arranged by size:
2568 890 106
BmrI ACTGGGnnnn_n'
Cuts at: 0 1038 1736 3564
Size: 1038 698 1828
Fragments arranged by size:
1828 1038 698
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1670 1907 2127 3564
Size: 1670 237 220 1437
Fragments arranged by size:
1670 1437 237 220
Bpu10I CC'TnA_GC
Cuts at: 0 628 3322 3564
Size: 628 2694 242
Fragments arranged by size:
2694 628 242
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 1254 3564
Size: 364 890 2310
Fragments arranged by size:
2310 890 364
BsaBI GATnn'nnATC
Cuts at: 0 2099 2848 3564
Size: 2099 749 716
Fragments arranged by size:
2099 749 716
BsaWI w'CCGG_w
Cuts at: 0 3116 3564
Size: 3116 448
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 852 882 1462 1492 1646 1676 1880 1910
Size: 852 30 580 30 154 30 204 30
Cuts at: 1910 3530 3560 3564
Size: 1620 30 4
Fragments arranged by size:
1620 852 580 204 154 30 30 30
30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1576 2305 3564
Size: 1576 729 1259
Fragments arranged by size:
1576 1259 729
BseYI C'CCAG_C
Cuts at: 0 1357 2104 2481 2549 3564
Size: 1357 747 377 68 1015
Fragments arranged by size:
1357 1015 747 377 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 746 1451 1918 2590 2770 2886 3564
Size: 251 495 705 467 672 180 116 678
Fragments arranged by size:
705 678 672 495 467 251 180 116
BsiHKAI G_wGCw'C
Cuts at: 0 1416 2548 3176 3291 3564
Size: 1416 1132 628 115 273
Fragments arranged by size:
1416 1132 628 273 115
BsmI GAATG_Cn'
Cuts at: 0 380 2981 3131 3564
Size: 380 2601 150 433
Fragments arranged by size:
2601 433 380 150
Bsp1286I G_dGCh'C
Cuts at: 0 1416 2548 3176 3291 3347 3564
Size: 1416 1132 628 115 56 217
Fragments arranged by size:
1416 1132 628 217 115 56
BspEI T'CCGG_A
Cuts at: 0 3116 3564
Size: 3116 448
BspHI T'CATG_A
Cuts at: 0 35 713 1785 3564
Size: 35 678 1072 1779
Fragments arranged by size:
1779 1072 678 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2357 2742 3012 3564
Size: 2357 385 270 552
Fragments arranged by size:
2357 552 385 270
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1584 3564
Size: 1584 1980
BstBI TT'CG_AA
Cuts at: 0 1944 3564
Size: 1944 1620
BstEII G'GTnAC_C
Cuts at: 0 934 2025 3564
Size: 934 1091 1539
Fragments arranged by size:
1539 1091 934
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1253 1651 2133 3564
Size: 1253 398 482 1431
Fragments arranged by size:
1431 1253 482 398
BstYI r'GATC_y
Cuts at: 0 222 927 977 1110 1256 1581 2434 2814
Size: 222 705 50 133 146 325 853 380
Cuts at: 2814 3564
Size: 750
Fragments arranged by size:
853 750 705 380 325 222 146 133
50
BtgI C'CryG_G
Cuts at: 0 2285 3564
Size: 2285 1279
BtsI GCAGTG_nn'
Cuts at: 0 194 2156 2251 3564
Size: 194 1962 95 1313
Fragments arranged by size:
1962 1313 194 95
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 404 439 3564
Size: 404 35 3125
Fragments arranged by size:
3125 404 35
DraI TTT'AAA
Cuts at: 0 678 2885 3564
Size: 678 2207 679
Fragments arranged by size:
2207 679 678
DrdI GACnn_nn'nnGTC
Cuts at: 0 1745 3564
Size: 1745 1819
EaeI y'GGCC_r
Cuts at: 0 1335 1664 3564
Size: 1335 329 1900
Fragments arranged by size:
1900 1335 329
EarI CTCTTCn'nnn_
Cuts at: 0 348 2211 3564
Size: 348 1863 1353
Fragments arranged by size:
1863 1353 348
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2427 3564
Size: 2427 1137
EcoICRI GAG'CTC
Cuts at: 0 2546 3174 3289 3564
Size: 2546 628 115 275
Fragments arranged by size:
2546 628 275 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1670 1735 1907 2127 2459 3218 3564
Size: 1670 65 172 220 332 759 346
Fragments arranged by size:
1670 759 346 332 220 172 65
EcoO109I rG'GnC_Cy
Cuts at: 0 730 1738 2339 2597 3564
Size: 730 1008 601 258 967
Fragments arranged by size:
1008 967 730 601 258
EcoRI G'AATT_C
Cuts at: 0 3094 3187 3564
Size: 3094 93 377
Fragments arranged by size:
3094 377 93
EcoRV GAT'ATC
Cuts at: 0 147 1293 3564
Size: 147 1146 2271
Fragments arranged by size:
2271 1146 147
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 703 946 3564
Size: 703 243 2618
Fragments arranged by size:
2618 703 243
FspI TGC'GCA
Cuts at: 0 3130 3564
Size: 3130 434
FspAI rTGC'GCAy
Cuts at: 0 3130 3564
Size: 3130 434
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 183 215 818 850 2557 2589 3564
Size: 183 32 603 32 1707 32 975
Fragments arranged by size:
1707 975 603 183 32 32 32
HindIII A'AGCT_T
Cuts at: 0 398 3564
Size: 398 3166
KpnI G_GTAC'C
Cuts at: 0 132 3564
Size: 132 3432
MfeI C'AATT_G
Cuts at: 0 1224 1251 2799 2903 3564
Size: 1224 27 1548 104 661
Fragments arranged by size:
1548 1224 661 104 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 838 861 1013 1505 2763 3564
Size: 838 23 152 492 1258 801
Fragments arranged by size:
1258 838 801 492 152 23
MscI TGG'CCA
Cuts at: 0 1337 3564
Size: 1337 2227
MslI CAynn'nnrTG
Cuts at: 0 154 1547 3436 3524 3564
Size: 154 1393 1889 88 40
Fragments arranged by size:
1889 1393 154 88 40
MspA1I CmG'CkG
Cuts at: 0 1612 2006 2108 2198 3140 3420 3564
Size: 1612 394 102 90 942 280 144
Fragments arranged by size:
1612 942 394 280 144 102 90
NcoI C'CATG_G
Cuts at: 0 2285 3564
Size: 2285 1279
NspI r_CATG'y
Cuts at: 0 32 255 413 844 3564
Size: 32 223 158 431 2720
Fragments arranged by size:
2720 431 223 158 32
PasI CC'CwG_GG
Cuts at: 0 3245 3564
Size: 3245 319
PciI A'CATG_T
Cuts at: 0 28 840 3564
Size: 28 812 2724
Fragments arranged by size:
2724 812 28
PflMI CCAn_nnn'nTGG
Cuts at: 0 1968 3564
Size: 1968 1596
PfoI T'CCnGG_A
Cuts at: 0 169 384 3564
Size: 169 215 3180
Fragments arranged by size:
3180 215 169
PpuMI rG'GwC_Cy
Cuts at: 0 730 1738 3564
Size: 730 1008 1826
Fragments arranged by size:
1826 1008 730
PshAI GACnn'nnGTC
Cuts at: 0 3420 3564
Size: 3420 144
PspXI vC'TCGA_Gb
Cuts at: 0 1415 1993 3564
Size: 1415 578 1571
Fragments arranged by size:
1571 1415 578
PstI C_TGCA'G
Cuts at: 0 143 1589 2113 3342 3429 3564
Size: 143 1446 524 1229 87 135
Fragments arranged by size:
1446 1229 524 143 135 87
PvuII CAG'CTG
Cuts at: 0 1612 2006 2108 3140 3420 3564
Size: 1612 394 102 1032 280 144
Fragments arranged by size:
1612 1032 394 280 144 102
SacI G_AGCT'C
Cuts at: 0 2548 3176 3291 3564
Size: 2548 628 115 273
Fragments arranged by size:
2548 628 273 115
SapI GCTCTTCn'nnn_
Cuts at: 0 2211 3564
Size: 2211 1353
ScaI AGT'ACT
Cuts at: 0 110 3564
Size: 110 3454
SexAI A'CCwGG_T
Cuts at: 0 2860 3564
Size: 2860 704
SfcI C'TryA_G
Cuts at: 0 139 557 1087 1585 2109 2222 2522 3152
Size: 139 418 530 498 524 113 300 630
Cuts at: 3152 3338 3425 3564
Size: 186 87 139
Fragments arranged by size:
630 530 524 498 418 300 186 139
139 113 87
SmlI C'TyrA_G
Cuts at: 0 343 699 1269 1415 1993 3564
Size: 343 356 570 146 578 1571
Fragments arranged by size:
1571 578 570 356 343 146
SpeI A'CTAG_T
Cuts at: 0 2713 3564
Size: 2713 851
SphI G_CATG'C
Cuts at: 0 413 3564
Size: 413 3151
StyI C'CwwG_G
Cuts at: 0 822 1403 2285 3316 3564
Size: 822 581 882 1031 248
Fragments arranged by size:
1031 882 822 581 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 415 1416 3564
Size: 415 1001 2148
Fragments arranged by size:
2148 1001 415
TatI w'GTAC_w
Cuts at: 0 108 2631 2716 3564
Size: 108 2523 85 848
Fragments arranged by size:
2523 848 108 85
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 136 168 852 884 1646 1678 1880 1912
Size: 136 32 684 32 762 32 202 32
Cuts at: 1912 3564
Size: 1652
Fragments arranged by size:
1652 762 684 202 136 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1639 3564
Size: 1639 1925
XbaI T'CTAG_A
Cuts at: 0 1818 3564
Size: 1818 1746
XhoI C'TCGA_G
Cuts at: 0 1415 1993 3564
Size: 1415 578 1571
Fragments arranged by size:
1571 1415 578
XmnI GAAnn'nnTTC
Cuts at: 0 806 1055 3564
Size: 806 249 2509
Fragments arranged by size:
2509 806 249
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AhdI AleI AlfI
AlwNI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BglII BlpI BmgBI BmrI BpmI Bpu10I
BpuEI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BstAPI BstBI BstEII
BstXI BstYI BtgI BtsI CspCI DraI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI FspAI Hin4I HindIII KpnI MfeI MmeI MscI
MslI MspA1I NcoI NspI PasI PciI PflMI PfoI
PpuMI PshAI PspXI PstI PvuII SacI SapI ScaI
SexAI SfcI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AloI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BclI BfrBI
BglI Bme1580I BmtI BplI BsaI BsaAI BsaHI BsiEI
BsiWI BsmBI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstZ17I Bsu36I BtgZI ClaI DraIII EagI EcoNI FseI
HaeII HincII HpaI KasI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI
PpiI PsiI PspOMI PsrI PvuI RsrII SacII SalI
SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI
SrfI SspI StuI SwaI XcmI XmaI ZraI