Restriction map for
pF1KB9488
(Linear) (Six-base) MAPSORT of: KIBB9488.seq Check: 6198 from: 1 to: 3399
KIBB9488.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AccI GT'mk_AC
Cuts at: 0 195 3366 3399
Size: 195 3171 33
Fragments arranged by size:
3171 195 33
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 681 751 1282 1519 1852 2039 2045
Size: 446 235 70 531 237 333 187 6
Cuts at: 2045 3399
Size: 1354
Fragments arranged by size:
1354 531 446 333 237 235 187 70
6
AflII C'TTAA_G
Cuts at: 0 2751 3399
Size: 2751 648
AhdI GACnn_n'nnGTC
Cuts at: 0 1590 3399
Size: 1590 1809
AleI CACnn'nnGTG
Cuts at: 0 358 3399
Size: 358 3041
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 498 3399
Size: 498 2901
AlwNI CAG_nnn'CTG
Cuts at: 0 1689 3267 3399
Size: 1689 1578 132
Fragments arranged by size:
1689 1578 132
ApaI G_GGCC'C
Cuts at: 0 2772 3399
Size: 2772 627
ApoI r'AATT_y
Cuts at: 0 634 1244 1703 2677 3140 3399
Size: 634 610 459 974 463 259
Fragments arranged by size:
974 634 610 463 459 259
AvaI C'yCGr_G
Cuts at: 0 2830 3399
Size: 2830 569
BanI G'GyrC_C
Cuts at: 0 276 3399
Size: 276 3123
BanII G_rGCy'C
Cuts at: 0 7 777 2772 2795 3155 3399
Size: 7 770 1995 23 360 244
Fragments arranged by size:
1995 770 360 244 23 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 2416 2884 3399
Size: 2416 468 515
Fragments arranged by size:
2416 515 468
BbvCI CC'TCA_GC
Cuts at: 0 2967 3399
Size: 2967 432
BciVI GTATCCnnnnn_n'
Cuts at: 0 194 1136 3211 3250 3399
Size: 194 942 2075 39 149
Fragments arranged by size:
2075 942 194 149 39
BclI T'GATC_A
Cuts at: 0 2237 3399
Size: 2237 1162
BfrBI ATG'CAT
Cuts at: 0 490 949 3399
Size: 490 459 2450
Fragments arranged by size:
2450 490 459
BglI GCCn_nnn'nGGC
Cuts at: 0 2152 3399
Size: 2152 1247
BglII A'GATC_T
Cuts at: 0 830 3054 3399
Size: 830 2224 345
Fragments arranged by size:
2224 830 345
Bme1580I G_kGCm'C
Cuts at: 0 1345 2772 3399
Size: 1345 1427 627
Fragments arranged by size:
1427 1345 627
BmrI ACTGGGnnnn_n'
Cuts at: 0 321 2214 3149 3399
Size: 321 1893 935 250
Fragments arranged by size:
1893 935 321 250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2779 2885 3399
Size: 2779 106 514
Fragments arranged by size:
2779 514 106
Bpu10I CC'TnA_GC
Cuts at: 0 2180 2967 3322 3399
Size: 2180 787 355 77
Fragments arranged by size:
2180 787 355 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1943 2822 3399
Size: 1943 879 577
Fragments arranged by size:
1943 879 577
BsaI GGTCTCn'nnnn_
Cuts at: 0 3184 3399
Size: 3184 215
BsaAI yAC'GTr
Cuts at: 0 2418 3018 3199 3399
Size: 2418 600 181 200
Fragments arranged by size:
2418 600 200 181
BsaBI GATnn'nnATC
Cuts at: 0 1826 3399
Size: 1826 1573
BsaHI Gr'CG_yC
Cuts at: 0 113 3399
Size: 113 3286
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 437 467 3188 3218 3399
Size: 437 30 2721 30 181
Fragments arranged by size:
2721 437 181 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2129 2132 2135 3399
Size: 2129 3 3 1264
Fragments arranged by size:
2129 1264 3 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 1783 2273 3399
Size: 944 839 490 1126
Fragments arranged by size:
1126 944 839 490
BsiHKAI G_wGCw'C
Cuts at: 0 167 1507 2702 2795 3399
Size: 167 1340 1195 93 604
Fragments arranged by size:
1340 1195 604 167 93
BsmI GAATG_Cn'
Cuts at: 0 2887 3054 3399
Size: 2887 167 345
Fragments arranged by size:
2887 345 167
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2074 3399
Size: 2074 1325
Bsp1286I G_dGCh'C
Cuts at: 0 7 167 777 1345 1507 2702 2772 2795
Size: 7 160 610 568 162 1195 70 23
Cuts at: 2795 3155 3399
Size: 360 244
Fragments arranged by size:
1195 610 568 360 244 162 160 70
23 7
BsrBI CCG'CTC
Cuts at: 0 1077 3399
Size: 1077 2322
BsrDI GCAATG_nn'
Cuts at: 0 455 492 1317 2082 2978 3399
Size: 455 37 825 765 896 421
Fragments arranged by size:
896 825 765 455 421 37
BsrFI r'CCGG_y
Cuts at: 0 41 3399
Size: 41 3358
BsrGI T'GTAC_A
Cuts at: 0 759 2265 3399
Size: 759 1506 1134
Fragments arranged by size:
1506 1134 759
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1066 3225 3399
Size: 1066 2159 174
Fragments arranged by size:
2159 1066 174
BstEII G'GTnAC_C
Cuts at: 0 172 3399
Size: 172 3227
BstXI CCAn_nnnn'nTGG
Cuts at: 0 360 976 3399
Size: 360 616 2423
Fragments arranged by size:
2423 616 360
BstYI r'GATC_y
Cuts at: 0 236 830 2061 3054 3399
Size: 236 594 1231 993 345
Fragments arranged by size:
1231 993 594 345 236
BtsI GCAGTG_nn'
Cuts at: 0 413 1771 3399
Size: 413 1358 1628
Fragments arranged by size:
1628 1358 413
DraI TTT'AAA
Cuts at: 0 2102 3399
Size: 2102 1297
DraIII CAC_nnn'GTG
Cuts at: 0 14 543 3399
Size: 14 529 2856
Fragments arranged by size:
2856 529 14
DrdI GACnn_nn'nnGTC
Cuts at: 0 2328 3399
Size: 2328 1071
EarI CTCTTCn'nnn_
Cuts at: 0 609 1307 2429 3399
Size: 609 698 1122 970
Fragments arranged by size:
1122 970 698 609
EcoICRI GAG'CTC
Cuts at: 0 2793 3399
Size: 2793 606
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 681 751 1282 1519 1852 2039 2045
Size: 446 235 70 531 237 333 187 6
Cuts at: 2045 2779 2885 3399
Size: 734 106 514
Fragments arranged by size:
734 531 514 446 333 237 235 187
106 70 6
EcoO109I rG'GnC_Cy
Cuts at: 0 2769 2963 3399
Size: 2769 194 436
Fragments arranged by size:
2769 436 194
EcoRI G'AATT_C
Cuts at: 0 1244 3399
Size: 1244 2155
EcoRV GAT'ATC
Cuts at: 0 1662 3399
Size: 1662 1737
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3138 3399
Size: 3138 261
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 123 155 437 469 1576 1608 3046 3078
Size: 123 32 282 32 1107 32 1438 32
Cuts at: 3078 3185 3217 3367 3399
Size: 107 32 150 32
Fragments arranged by size:
1438 1107 282 150 123 107 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 0 1013 3367 3399
Size: 1013 2354 32
Fragments arranged by size:
2354 1013 32
HindIII A'AGCT_T
Cuts at: 0 1112 1412 3399
Size: 1112 300 1987
Fragments arranged by size:
1987 1112 300
HpaI GTT'AAC
Cuts at: 0 1013 3399
Size: 1013 2386
MfeI C'AATT_G
Cuts at: 0 872 2642 3399
Size: 872 1770 757
Fragments arranged by size:
1770 872 757
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2463 3399
Size: 2463 936
MslI CAynn'nnrTG
Cuts at: 0 216 358 1802 2627 3047 3399
Size: 216 142 1444 825 420 352
Fragments arranged by size:
1444 825 420 352 216 142
MspA1I CmG'CkG
Cuts at: 0 1260 2371 2508 3267 3318 3399
Size: 1260 1111 137 759 51 81
Fragments arranged by size:
1260 1111 759 137 81 51
NaeI GCC'GGC
Cuts at: 0 43 3399
Size: 43 3356
NdeI CA'TA_TG
Cuts at: 0 213 3399
Size: 213 3186
NgoMIV G'CCGG_C
Cuts at: 0 41 3399
Size: 41 3358
NsiI A_TGCA'T
Cuts at: 0 492 951 3399
Size: 492 459 2448
Fragments arranged by size:
2448 492 459
NspI r_CATG'y
Cuts at: 0 494 586 3399
Size: 494 92 2813
Fragments arranged by size:
2813 494 92
PasI CC'CwG_GG
Cuts at: 0 2765 3399
Size: 2765 634
PflMI CCAn_nnn'nTGG
Cuts at: 0 14 543 2908 3399
Size: 14 529 2365 491
Fragments arranged by size:
2365 529 491 14
PmlI CAC'GTG
Cuts at: 0 2418 3018 3399
Size: 2418 600 381
Fragments arranged by size:
2418 600 381
PpuMI rG'GwC_Cy
Cuts at: 0 2963 3399
Size: 2963 436
PsiI TTA'TAA
Cuts at: 0 86 556 820 2218 3399
Size: 86 470 264 1398 1181
Fragments arranged by size:
1398 1181 470 264 86
PspOMI G'GGCC_C
Cuts at: 0 2768 3399
Size: 2768 631
PspXI vC'TCGA_Gb
Cuts at: 0 2830 3399
Size: 2830 569
PstI C_TGCA'G
Cuts at: 0 477 3399
Size: 477 2922
PvuII CAG'CTG
Cuts at: 0 1260 2508 3267 3318 3399
Size: 1260 1248 759 51 81
Fragments arranged by size:
1260 1248 759 81 51
SacI G_AGCT'C
Cuts at: 0 2795 3399
Size: 2795 604
SalI G'TCGA_C
Cuts at: 0 3365 3399
Size: 3365 34
SfcI C'TryA_G
Cuts at: 0 473 834 1490 3106 3399
Size: 473 361 656 1616 293
Fragments arranged by size:
1616 656 473 361 293
SmlI C'TyrA_G
Cuts at: 0 1958 2751 2830 2837 3399
Size: 1958 793 79 7 562
Fragments arranged by size:
1958 793 562 79 7
SnaBI TAC'GTA
Cuts at: 0 3199 3399
Size: 3199 200
SspI AAT'ATT
Cuts at: 0 789 1387 1725 2916 3399
Size: 789 598 338 1191 483
Fragments arranged by size:
1191 789 598 483 338
StuI AGG'CCT
Cuts at: 0 973 3399
Size: 973 2426
StyI C'CwwG_G
Cuts at: 0 1557 3399
Size: 1557 1842
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1715 2150 3133 3399
Size: 1715 435 983 266
Fragments arranged by size:
1715 983 435 266
TatI w'GTAC_w
Cuts at: 0 519 759 1740 2265 3399
Size: 519 240 981 525 1134
Fragments arranged by size:
1134 981 525 519 240
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2078 2110 2460 2492 3399
Size: 2078 32 350 32 907
Fragments arranged by size:
2078 907 350 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2817 3399
Size: 2817 582
XhoI C'TCGA_G
Cuts at: 0 2830 3399
Size: 2830 569
Enzymes that do cut:
AccI AcuI AflII AhdI AleI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BbsI BbvCI BciVI BclI
BfrBI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BsrBI BsrDI BsrFI BsrGI BstAPI
BstEII BstXI BstYI BtsI DraI DraIII DrdI EarI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI Hin4I HincII
HindIII HpaI MfeI MmeI MslI MspA1I NaeI NdeI
NgoMIV NsiI NspI PasI PflMI PmlI PpuMI PsiI
PspOMI PspXI PstI PvuII SacI SalI SfcI SmlI
SnaBI SspI StuI StyI TaqII TatI TstI XcmI
XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BcgI BlpI BmgBI BmtI BplI BsaWI BseYI BsiEI
BsiWI BspEI BspHI BspMI BssHII BssSI BstBI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI EaeI EagI EciI
EcoNI FseI FspI FspAI HaeII KasI KpnI MluI
MscI NarI NcoI NheI NotI NruI PacI PciI
PfoI PmeI PpiI PshAI PsrI PvuI RsrII SacII
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SpeI SphI SrfI SwaI Tth111I XbaI
XmaI XmnI ZraI