Restriction map for
pF1KB9487
(Linear) (Six-base) MAPSORT of: KIBB9487.seq Check: 7950 from: 1 to: 3561
KIBB9487.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AccI GT'mk_AC
Cuts at: 0 793 1514 3518 3561
Size: 793 721 2004 43
Fragments arranged by size:
2004 793 721 43
Acc65I G'GTAC_C
Cuts at: 0 1569 3561
Size: 1569 1992
AclI AA'CG_TT
Cuts at: 0 630 3561
Size: 630 2931
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 265 310 1674 2719 3561
Size: 34 231 45 1364 1045 842
Fragments arranged by size:
1364 1045 842 231 45 34
AflII C'TTAA_G
Cuts at: 0 1675 3561
Size: 1675 1886
AflIII A'CryG_T
Cuts at: 0 2963 3561
Size: 2963 598
AleI CACnn'nnGTG
Cuts at: 0 2964 3362 3561
Size: 2964 398 199
Fragments arranged by size:
2964 398 199
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2671 2703 3561
Size: 2671 32 858
Fragments arranged by size:
2671 858 32
AlwNI CAG_nnn'CTG
Cuts at: 0 159 601 1395 3561
Size: 159 442 794 2166
Fragments arranged by size:
2166 794 442 159
ApaI G_GGCC'C
Cuts at: 0 2954 3561
Size: 2954 607
ApaLI G'TGCA_C
Cuts at: 0 102 3561
Size: 102 3459
ApoI r'AATT_y
Cuts at: 0 262 936 2744 3561
Size: 262 674 1808 817
Fragments arranged by size:
1808 817 674 262
AseI AT'TA_AT
Cuts at: 0 2888 3561
Size: 2888 673
AvaI C'yCGr_G
Cuts at: 0 1278 2029 2627 2666 3561
Size: 1278 751 598 39 895
Fragments arranged by size:
1278 895 751 598 39
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1224 1257 3561
Size: 1224 33 2304
Fragments arranged by size:
2304 1224 33
BamHI G'GATC_C
Cuts at: 0 1364 3561
Size: 1364 2197
BanI G'GyrC_C
Cuts at: 0 1478 1569 2160 3325 3338 3561
Size: 1478 91 591 1165 13 223
Fragments arranged by size:
1478 1165 591 223 91 13
BanII G_rGCy'C
Cuts at: 0 812 1880 2289 2618 2954 3561
Size: 812 1068 409 329 336 607
Fragments arranged by size:
1068 812 607 409 336 329
BbsI GAAGACnn'nnnn_
Cuts at: 0 744 1825 2092 2516 3561
Size: 744 1081 267 424 1045
Fragments arranged by size:
1081 1045 744 424 267
BbvCI CC'TCA_GC
Cuts at: 0 2341 3457 3561
Size: 2341 1116 104
Fragments arranged by size:
2341 1116 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 78 112 1287 1321 3561
Size: 78 34 1175 34 2240
Fragments arranged by size:
2240 1175 78 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1236 1252 2875 3561
Size: 1236 16 1623 686
Fragments arranged by size:
1623 1236 686 16
BclI T'GATC_A
Cuts at: 0 2537 3561
Size: 2537 1024
BlpI GC'TnA_GC
Cuts at: 0 3093 3561
Size: 3093 468
Bme1580I G_kGCm'C
Cuts at: 0 106 1310 2165 2205 2954 3449 3561
Size: 106 1204 855 40 749 495 112
Fragments arranged by size:
1204 855 749 495 112 106 40
BmgBI CAC'GTC
Cuts at: 0 1023 3561
Size: 1023 2538
BmtI G_CTAG'C
Cuts at: 0 424 3561
Size: 424 3137
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1059 1409 1854 3561
Size: 1059 350 445 1707
Fragments arranged by size:
1707 1059 445 350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 181 253 556 990 1846 1948 2135
Size: 160 21 72 303 434 856 102 187
Cuts at: 2135 2593 3534 3561
Size: 458 941 27
Fragments arranged by size:
941 856 458 434 303 187 160 102
72 27 21
Bpu10I CC'TnA_GC
Cuts at: 0 46 2341 3457 3561
Size: 46 2295 1116 104
Fragments arranged by size:
2295 1116 104 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 1957 3561
Size: 458 1499 1604
Fragments arranged by size:
1604 1499 458
BsaI GGTCTCn'nnnn_
Cuts at: 0 2475 3561
Size: 2475 1086
BsaAI yAC'GTr
Cuts at: 0 2966 3561
Size: 2966 595
BsaBI GATnn'nnATC
Cuts at: 0 613 2536 3267 3561
Size: 613 1923 731 294
Fragments arranged by size:
1923 731 613 294
BsaHI Gr'CG_yC
Cuts at: 0 957 3561
Size: 957 2604
BsaWI w'CCGG_w
Cuts at: 0 1461 1842 3561
Size: 1461 381 1719
Fragments arranged by size:
1719 1461 381
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2993 3023 3274 3304 3561
Size: 2993 30 251 30 257
Fragments arranged by size:
2993 257 251 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 553 2047 2146 2149 2152 2155 2158 2851
Size: 553 1494 99 3 3 3 3 693
Cuts at: 2851 3046 3049 3295 3454 3561
Size: 195 3 246 159 107
Fragments arranged by size:
1494 693 553 246 195 159 107 99
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 2060 2204 3367 3561
Size: 2060 144 1163 194
Fragments arranged by size:
2060 1163 194 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 906 1825 3561
Size: 906 919 1736
Fragments arranged by size:
1736 919 906
BsiHKAI G_wGCw'C
Cuts at: 0 106 812 1040 1615 3392 3561
Size: 106 706 228 575 1777 169
Fragments arranged by size:
1777 706 575 228 169 106
BsmI GAATG_Cn'
Cuts at: 0 139 3561
Size: 139 3422
BsmBI CGTCTCn'nnnn_
Cuts at: 0 166 3561
Size: 166 3395
Bsp1286I G_dGCh'C
Cuts at: 0 106 812 1040 1310 1615 1880 2165 2205
Size: 106 706 228 270 305 265 285 40
Cuts at: 2205 2289 2618 2954 3392 3449 3561
Size: 84 329 336 438 57 112
Fragments arranged by size:
706 438 336 329 305 285 270 265
228 112 106 84 57 40
BspHI T'CATG_A
Cuts at: 0 3485 3561
Size: 3485 76
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 365 1731 3561
Size: 365 1366 1830
Fragments arranged by size:
1830 1366 365
BsrBI CCG'CTC
Cuts at: 0 2454 3561
Size: 2454 1107
BsrDI GCAATG_nn'
Cuts at: 0 96 2286 3561
Size: 96 2190 1275
Fragments arranged by size:
2190 1275 96
BsrFI r'CCGG_y
Cuts at: 0 424 974 1286 2388 3335 3382 3561
Size: 424 550 312 1102 947 47 179
Fragments arranged by size:
1102 947 550 424 312 179 47
BsrGI T'GTAC_A
Cuts at: 0 397 611 687 1451 3561
Size: 397 214 76 764 2110
Fragments arranged by size:
2110 764 397 214 76
BssSI C'ACGA_G
Cuts at: 0 2050 3561
Size: 2050 1511
BstAPI GCAn_nnn'nTGC
Cuts at: 0 56 3561
Size: 56 3505
BstBI TT'CG_AA
Cuts at: 0 3132 3561
Size: 3132 429
BstEII G'GTnAC_C
Cuts at: 0 1791 3117 3561
Size: 1791 1326 444
Fragments arranged by size:
1791 1326 444
BstXI CCAn_nnnn'nTGG
Cuts at: 0 804 3561
Size: 804 2757
BstYI r'GATC_y
Cuts at: 0 1364 1443 2168 3284 3561
Size: 1364 79 725 1116 277
Fragments arranged by size:
1364 1116 725 277 79
Bsu36I CC'TnA_GG
Cuts at: 0 3466 3561
Size: 3466 95
BtgI C'CryG_G
Cuts at: 0 1711 1899 1991 2321 2675 3017 3158 3561
Size: 1711 188 92 330 354 342 141 403
Fragments arranged by size:
1711 403 354 342 330 188 141 92
BtsI GCAGTG_nn'
Cuts at: 0 3023 3561
Size: 3023 538
DraI TTT'AAA
Cuts at: 0 1136 3561
Size: 1136 2425
DraIII CAC_nnn'GTG
Cuts at: 0 1608 3561
Size: 1608 1953
DrdI GACnn_nn'nnGTC
Cuts at: 0 1974 3561
Size: 1974 1587
EaeI y'GGCC_r
Cuts at: 0 1625 1714 1745 1886 2324 3229 3561
Size: 1625 89 31 141 438 905 332
Fragments arranged by size:
1625 905 438 332 141 89 31
EarI CTCTTCn'nnn_
Cuts at: 0 518 818 1351 1693 2263 2578 2639 2708
Size: 518 300 533 342 570 315 61 69
Cuts at: 2708 3561
Size: 853
Fragments arranged by size:
853 570 533 518 342 315 300 69
61
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 10 3561
Size: 10 3551
EcoICRI GAG'CTC
Cuts at: 0 810 3561
Size: 810 2751
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 160 181 253 265 310 556 990
Size: 34 126 21 72 12 45 246 434
Cuts at: 990 1674 1846 1948 2135 2593 2719 3534 3561
Size: 684 172 102 187 458 126 815 27
Fragments arranged by size:
815 684 458 434 246 187 172 126
126 102 72 45 34 27 21 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 523 1314 3561
Size: 523 791 2247
Fragments arranged by size:
2247 791 523
EcoO109I rG'GnC_Cy
Cuts at: 0 2055 2402 2950 2951 3561
Size: 2055 347 548 1 610
Fragments arranged by size:
2055 610 548 347 1
EcoRV GAT'ATC
Cuts at: 0 346 852 3561
Size: 346 506 2709
Fragments arranged by size:
2709 506 346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 75 394 2106 2326 3561
Size: 75 319 1712 220 1235
Fragments arranged by size:
1712 1235 319 220 75
HaeII r_GCGC'y
Cuts at: 0 36 1595 1920 3561
Size: 36 1559 325 1641
Fragments arranged by size:
1641 1559 325 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 638 670 2023 2055 2529 2561 3274 3306
Size: 638 32 1353 32 474 32 713 32
Cuts at: 3306 3486 3518 3561
Size: 180 32 43
Fragments arranged by size:
1353 713 638 474 180 43 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 794 1182 2397 3561
Size: 794 388 1215 1164
Fragments arranged by size:
1215 1164 794 388
KpnI G_GTAC'C
Cuts at: 0 1573 3561
Size: 1573 1988
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1256 3561
Size: 1256 2305
MscI TGG'CCA
Cuts at: 0 1627 1747 2326 3231 3561
Size: 1627 120 579 905 330
Fragments arranged by size:
1627 905 579 330 120
MslI CAynn'nnrTG
Cuts at: 0 802 845 1082 2368 2905 2964 3362 3561
Size: 802 43 237 1286 537 59 398 199
Fragments arranged by size:
1286 802 537 398 237 199 59 43
MspA1I CmG'CkG
Cuts at: 0 1638 1772 2208 2592 3561
Size: 1638 134 436 384 969
Fragments arranged by size:
1638 969 436 384 134
NaeI GCC'GGC
Cuts at: 0 426 2390 3561
Size: 426 1964 1171
Fragments arranged by size:
1964 1171 426
NcoI C'CATG_G
Cuts at: 0 1991 2321 2675 3017 3561
Size: 1991 330 354 342 544
Fragments arranged by size:
1991 544 354 342 330
NgoMIV G'CCGG_C
Cuts at: 0 424 2388 3561
Size: 424 1964 1173
Fragments arranged by size:
1964 1173 424
NheI G'CTAG_C
Cuts at: 0 420 3561
Size: 420 3141
NspI r_CATG'y
Cuts at: 0 1635 1674 2373 3318 3561
Size: 1635 39 699 945 243
Fragments arranged by size:
1635 945 699 243 39
PasI CC'CwG_GG
Cuts at: 0 1246 2946 3561
Size: 1246 1700 615
Fragments arranged by size:
1700 1246 615
PflMI CCAn_nnn'nTGG
Cuts at: 0 159 1926 3561
Size: 159 1767 1635
Fragments arranged by size:
1767 1635 159
PfoI T'CCnGG_A
Cuts at: 0 1862 2012 2180 3561
Size: 1862 150 168 1381
Fragments arranged by size:
1862 1381 168 150
PmlI CAC'GTG
Cuts at: 0 2966 3561
Size: 2966 595
PpuMI rG'GwC_Cy
Cuts at: 0 2055 3561
Size: 2055 1506
PshAI GACnn'nnGTC
Cuts at: 0 2487 3561
Size: 2487 1074
PspOMI G'GGCC_C
Cuts at: 0 2950 3561
Size: 2950 611
PspXI vC'TCGA_Gb
Cuts at: 0 2627 3561
Size: 2627 934
PstI C_TGCA'G
Cuts at: 0 1643 2213 3030 3561
Size: 1643 570 817 531
Fragments arranged by size:
1643 817 570 531
PvuII CAG'CTG
Cuts at: 0 1638 2208 3561
Size: 1638 570 1353
Fragments arranged by size:
1638 1353 570
RsrII CG'GwC_CG
Cuts at: 0 3294 3561
Size: 3294 267
SacI G_AGCT'C
Cuts at: 0 812 3561
Size: 812 2749
SalI G'TCGA_C
Cuts at: 0 792 3561
Size: 792 2769
SexAI A'CCwGG_T
Cuts at: 0 407 1787 3561
Size: 407 1380 1774
Fragments arranged by size:
1774 1380 407
SfcI C'TryA_G
Cuts at: 0 1515 1639 1656 2209 3026 3360 3561
Size: 1515 124 17 553 817 334 201
Fragments arranged by size:
1515 817 553 334 201 124 17
SmaI CCC'GGG
Cuts at: 0 2668 3561
Size: 2668 893
SmlI C'TyrA_G
Cuts at: 0 473 1675 1972 2627 3561
Size: 473 1202 297 655 934
Fragments arranged by size:
1202 934 655 473 297
SpeI A'CTAG_T
Cuts at: 0 2440 3561
Size: 2440 1121
SphI G_CATG'C
Cuts at: 0 3318 3561
Size: 3318 243
StuI AGG'CCT
Cuts at: 0 1216 2570 3561
Size: 1216 1354 991
Fragments arranged by size:
1354 1216 991
StyI C'CwwG_G
Cuts at: 0 693 1991 2263 2321 2357 2675 3017 3258
Size: 693 1298 272 58 36 318 342 241
Cuts at: 3258 3561
Size: 303
Fragments arranged by size:
1298 693 342 318 303 272 241 58
36
SwaI ATTT'AAAT
Cuts at: 0 1136 3561
Size: 1136 2425
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 213 1435 2959 3282 3561
Size: 213 1222 1524 323 279
Fragments arranged by size:
1524 1222 323 279 213
TatI w'GTAC_w
Cuts at: 0 397 611 687 1451 2195 3506 3561
Size: 397 214 76 764 744 1311 55
Fragments arranged by size:
1311 764 744 397 214 76 55
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2655 2687 2993 3025 3561
Size: 2655 32 306 32 536
Fragments arranged by size:
2655 536 306 32 32
XbaI T'CTAG_A
Cuts at: 0 2547 3561
Size: 2547 1014
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 317 3315 3364 3561
Size: 317 2998 49 197
Fragments arranged by size:
2998 317 197 49
XhoI C'TCGA_G
Cuts at: 0 2627 3561
Size: 2627 934
XmaI C'CCGG_G
Cuts at: 0 2666 3561
Size: 2666 895
XmnI GAAnn'nnTTC
Cuts at: 0 1822 2089 3138 3561
Size: 1822 267 1049 423
Fragments arranged by size:
1822 1049 423 267
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI AloI
AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BlpI
Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtsI DraI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII
Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NheI NspI PasI PflMI PfoI PmlI
PpuMI PshAI PspOMI PspXI PstI PvuII RsrII SacI
SalI SexAI SfcI SmaI SmlI SpeI SphI StuI
StyI SwaI TaqII TatI TstI XbaI XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AlfI AscI AsiSI
AvrII BbeI BfrBI BglI BglII BmrI BsiEI BsiWI
BspEI BssHII BstZ17I BtgZI ClaI CspCI EagI EcoRI
FseI FspI FspAI HindIII HpaI KasI MfeI MluI
NarI NdeI NotI NruI NsiI PacI PciI PmeI
PpiI PsiI PsrI PvuI SacII SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SrfI SspI
Tth111I ZraI