Restriction map for
pF1KB9484
(Linear) (Six-base) MAPSORT of: KIBB9484.seq Check: 5144 from: 1 to: 6135
KIBB9484.orf
With 182 enzymes: SgfI *
September 18, 2009 17:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4069 5409 5789 5889 6092 6135
Size: 4069 1340 380 100 203 43
Fragments arranged by size:
4069 1340 380 203 100 43
AatII G_ACGT'C
Cuts at: 0 3545 3963 4574 5429 6135
Size: 3545 418 611 855 706
Fragments arranged by size:
3545 855 706 611 418
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2103 2212 2869 3624 5295 5296 6135
Size: 2103 109 657 755 1671 1 839
Fragments arranged by size:
2103 1671 839 755 657 109 1
AflIII A'CryG_T
Cuts at: 0 185 1432 1715 3919 6135
Size: 185 1247 283 2204 2216
Fragments arranged by size:
2216 2204 1247 283 185
AhdI GACnn_n'nnGTC
Cuts at: 0 3471 5070 5430 6135
Size: 3471 1599 360 705
Fragments arranged by size:
3471 1599 705 360
AleI CACnn'nnGTG
Cuts at: 0 487 3394 6135
Size: 487 2907 2741
Fragments arranged by size:
2907 2741 487
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 721 1367 2004 4579 4976 6135
Size: 721 646 637 2575 397 1159
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 433 465 4984 5016 6135
Size: 433 32 4519 32 1119
Fragments arranged by size:
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AlwNI CAG_nnn'CTG
Cuts at: 0 773 894 1925 2055 2375 2874 2895 2988
Size: 773 121 1031 130 320 499 21 93
Cuts at: 2988 3441 4221 4587 4698 5394 5712 6135
Size: 453 780 366 111 696 318 423
Fragments arranged by size:
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320 318 130 121 111 93 21
ApaI G_GGCC'C
Cuts at: 0 25 1378 2662 2902 3587 3666 3864 4686
Size: 25 1353 1284 240 685 79 198 822
Cuts at: 4686 4904 6007 6135
Size: 218 1103 128
Fragments arranged by size:
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128 79 25
ApaLI G'TGCA_C
Cuts at: 0 1744 3616 6135
Size: 1744 1872 2519
Fragments arranged by size:
2519 1872 1744
ApoI r'AATT_y
Cuts at: 0 3534 4195 6135
Size: 3534 661 1940
Fragments arranged by size:
3534 1940 661
AvaI C'yCGr_G
Cuts at: 0 18 242 469 838 978 1036 1127 1405
Size: 18 224 227 369 140 58 91 278
Cuts at: 1405 1482 1564 1976 2342 2608 3253 3454 3659
Size: 77 82 412 366 266 645 201 205
Cuts at: 3659 3857 4244 4495 4619 4819 4881 5456 5499
Size: 198 387 251 124 200 62 575 43
Cuts at: 5499 5530 6135
Size: 31 605
Fragments arranged by size:
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266 251 227 224 205 201 200 198
140 124 91 82 77 62 58 43
31 18
BanI G'GyrC_C
Cuts at: 0 538 618 832 865 964 1013 1059 2407
Size: 538 80 214 33 99 49 46 1348
Cuts at: 2407 2557 3004 3120 3132 3645 3692 4087 4111
Size: 150 447 116 12 513 47 395 24
Cuts at: 4111 4361 4388 4396 4447 4537 4800 4861 5206
Size: 250 27 8 51 90 263 61 345
Cuts at: 5206 5356 5777 5941 6071 6135
Size: 150 421 164 130 64
Fragments arranged by size:
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250 214 164 150 150 130 116 99
90 80 64 61 51 49 47 46
33 27 24 12 8
BanII G_rGCy'C
Cuts at: 0 25 554 601 1193 1378 1632 2225 2662
Size: 25 529 47 592 185 254 593 437
Cuts at: 2662 2902 2972 3254 3260 3376 3587 3666 3864
Size: 240 70 282 6 116 211 79 198
Cuts at: 3864 4686 4771 4904 5806 5970 6007 6135
Size: 822 85 133 902 164 37 128
Fragments arranged by size:
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240 211 198 185 164 133 128 116
85 79 70 47 37 25 6
BbeI G_GCGC'C
Cuts at: 0 542 622 836 869 968 1017 1063 2561
Size: 542 80 214 33 99 49 46 1498
Cuts at: 2561 3008 3696 4091 4115 4392 4541 4804 5945
Size: 447 688 395 24 277 149 263 1141
Cuts at: 5945 6135
Size: 190
Fragments arranged by size:
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214 190 149 99 80 49 46 33
24
BbsI GAAGACnn'nnnn_
Cuts at: 0 918 2857 3355 5053 6135
Size: 918 1939 498 1698 1082
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 1782 6135
Size: 1782 4353
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 117 151 1163 1197 4810 4844 6135
Size: 117 34 1012 34 3613 34 1291
Fragments arranged by size:
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BciVI GTATCCnnnnn_n'
Cuts at: 0 5586 6135
Size: 5586 549
BclI T'GATC_A
Cuts at: 0 278 6135
Size: 278 5857
BglI GCCn_nnn'nGGC
Cuts at: 0 1057 2360 2525 2666 2870 3643 4076 4218
Size: 1057 1303 165 141 204 773 433 142
Cuts at: 4218 4733 5021 6135
Size: 515 288 1114
Fragments arranged by size:
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165 142 141
BlpI GC'TnA_GC
Cuts at: 0 709 2751 6135
Size: 709 2042 3384
Fragments arranged by size:
3384 2042 709
Bme1580I G_kGCm'C
Cuts at: 0 25 607 817 1186 1290 1378 1666 1715
Size: 25 582 210 369 104 88 288 49
Cuts at: 1715 1748 2301 2655 2662 2902 3125 3587 3620
Size: 33 553 354 7 240 223 462 33
Cuts at: 3620 3648 3666 3864 3956 4399 4686 4729 4846
Size: 28 18 198 92 443 287 43 117
Cuts at: 4846 4864 4904 5211 5780 5837 6007 6135
Size: 18 40 307 569 57 170 128
Fragments arranged by size:
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288 287 240 223 210 198 170 128
117 104 92 88 57 49 43 40
33 33 28 25 18 18 7
BmgBI CAC'GTC
Cuts at: 0 1433 3654 3782 3922 5432 5589 6135
Size: 1433 2221 128 140 1510 157 546
Fragments arranged by size:
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BmrI ACTGGGnnnn_n'
Cuts at: 0 335 2363 3866 4047 4436 6135
Size: 335 2028 1503 181 389 1699
Fragments arranged by size:
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BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 316 2766 4155 6135
Size: 316 2450 1389 1980
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 390 457 470 1564 1936 3433 3439
Size: 127 263 67 13 1094 372 1497 6
Cuts at: 3439 4228 4390 4540 4609 4720 4957 5011 5119
Size: 789 162 150 69 111 237 54 108
Cuts at: 5119 5206 5742 6109 6135
Size: 87 536 367 26
Fragments arranged by size:
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162 150 127 111 108 87 69 67
54 26 13 6
Bpu10I CC'TnA_GC
Cuts at: 0 1782 2977 4294 6135
Size: 1782 1195 1317 1841
Fragments arranged by size:
1841 1782 1317 1195
BsaI GGTCTCn'nnnn_
Cuts at: 0 287 1138 1169 1639 1792 4411 5184 5376
Size: 287 851 31 470 153 2619 773 192
Cuts at: 5376 6135
Size: 759
Fragments arranged by size:
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31
BsaAI yAC'GTr
Cuts at: 0 188 1304 1718 1743 1776 2229 3732 5292
Size: 188 1116 414 25 33 453 1503 1560
Cuts at: 5292 5409 6135
Size: 117 726
Fragments arranged by size:
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33 25
BsaHI Gr'CG_yC
Cuts at: 0 499 539 577 619 638 833 866 965
Size: 499 40 38 42 19 195 33 99
Cuts at: 965 1014 1060 1517 2305 2558 3005 3160 3352
Size: 49 46 457 788 253 447 155 192
Cuts at: 3352 3407 3425 3542 3693 3960 4088 4112 4389
Size: 55 18 117 151 267 128 24 277
Cuts at: 4389 4538 4571 4801 5426 5942 6095 6135
Size: 149 33 230 625 516 153 40
Fragments arranged by size:
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253 230 195 192 155 153 151 149
128 117 99 55 49 46 42 40
40 38 33 33 24 19 18
BsaWI w'CCGG_w
Cuts at: 0 2011 3530 3557 6135
Size: 2011 1519 27 2578
Fragments arranged by size:
2578 2011 1519 27
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 433 463 727 757 1799 1829
Size: 175 30 228 30 264 30 1042 30
Cuts at: 1829 2514 2544 3036 3066 4076 4106 4912 4942
Size: 685 30 492 30 1010 30 806 30
Cuts at: 4942 4984 5014 5788 5818 6135
Size: 42 30 774 30 317
Fragments arranged by size:
1042 1010 806 774 685 492 317 264
228 175 42 30 30 30 30 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 133 136 169 454 1126 1984 2755 2962
Size: 133 3 33 285 672 858 771 207
Cuts at: 2962 5919 6135
Size: 2957 216
Fragments arranged by size:
2957 858 771 672 285 216 207 133
33 3
BseYI C'CCAG_C
Cuts at: 0 607 1360 1688 1723 2418 2923 3022 3148
Size: 607 753 328 35 695 505 99 126
Cuts at: 3148 3943 3977 5017 5807 5937 6006 6135
Size: 795 34 1040 790 130 69 129
Fragments arranged by size:
1040 795 790 753 695 607 505 328
130 129 126 99 69 35 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 341 702 1432 1723 1737 1756 3128
Size: 199 142 361 730 291 14 19 1372
Cuts at: 3128 4309 4936 5436 6064 6135
Size: 1181 627 500 628 71
Fragments arranged by size:
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199 142 71 19 14
BsiEI CG_ry'CG
Cuts at: 0 9 34 788 1117 1251 2649 3718 4737
Size: 9 25 754 329 134 1398 1069 1019
Cuts at: 4737 5199 6135
Size: 462 936
Fragments arranged by size:
1398 1069 1019 936 754 462 329 134
25 9
BsiHKAI G_wGCw'C
Cuts at: 0 140 1483 1748 1840 1874 2437 2599 3514
Size: 140 1343 265 92 34 563 162 915
Cuts at: 3514 3620 3731 4833 5408 5720 6135
Size: 106 111 1102 575 312 415
Fragments arranged by size:
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162 140 111 106 92 34
BsmI GAATG_Cn'
Cuts at: 0 527 664 1471 1678 6135
Size: 527 137 807 207 4457
Fragments arranged by size:
4457 807 527 207 137
Bsp1286I G_dGCh'C
Cuts at: 0 25 140 554 601 607 817 1186 1193
Size: 25 115 414 47 6 210 369 7
Cuts at: 1193 1290 1378 1483 1632 1666 1715 1748 1840
Size: 97 88 105 149 34 49 33 92
Cuts at: 1840 1874 2225 2301 2437 2599 2655 2662 2902
Size: 34 351 76 136 162 56 7 240
Cuts at: 2902 2972 3125 3254 3260 3376 3514 3587 3620
Size: 70 153 129 6 116 138 73 33
Cuts at: 3620 3648 3666 3731 3864 3956 4399 4686 4729
Size: 28 18 65 133 92 443 287 43
Cuts at: 4729 4771 4833 4846 4864 4904 5211 5408 5720
Size: 42 62 13 18 40 307 197 312
Cuts at: 5720 5780 5806 5837 5970 6007 6135
Size: 60 26 31 133 37 128
Fragments arranged by size:
443 414 369 351 312 307 287 240
210 197 162 153 149 138 136 133
133 129 128 116 115 105 97 92
92 88 76 73 70 65 62 60
56 49 47 43 42 40 37 34
34 33 33 31 28 26 25 18
18 13 7 7 6 6
BspEI T'CCGG_A
Cuts at: 0 2011 3530 3557 6135
Size: 2011 1519 27 2578
Fragments arranged by size:
2578 2011 1519 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 763 832 4036 4069 5409 5431 5789 5889
Size: 763 69 3204 33 1340 22 358 100
Cuts at: 5889 6092 6135
Size: 203 43
Fragments arranged by size:
3204 1340 763 358 203 100 69 43
33 22
BsrBI CCG'CTC
Cuts at: 0 45 111 3215 4139 4524 5286 5742 6135
Size: 45 66 3104 924 385 762 456 393
Fragments arranged by size:
3104 924 762 456 393 385 66 45
BsrDI GCAATG_nn'
Cuts at: 0 3791 4716 5913 6135
Size: 3791 925 1197 222
Fragments arranged by size:
3791 1197 925 222
BsrFI r'CCGG_y
Cuts at: 0 4 8 79 1196 1340 2060 2991 3695
Size: 4 4 71 1117 144 720 931 704
Cuts at: 3695 4122 4317 4341 4540 5923 6068 6135
Size: 427 195 24 199 1383 145 67
Fragments arranged by size:
1383 1117 931 720 704 427 199 195
145 144 71 67 24 4 4
BsrGI T'GTAC_A
Cuts at: 0 2530 4229 6135
Size: 2530 1699 1906
Fragments arranged by size:
2530 1906 1699
BssHII G'CGCG_C
Cuts at: 0 99 101 778 1585 6135
Size: 99 2 677 807 4550
Fragments arranged by size:
4550 807 677 99 2
BssSI C'ACGA_G
Cuts at: 0 804 2598 5009 6135
Size: 804 1794 2411 1126
Fragments arranged by size:
2411 1794 1126 804
BstAPI GCAn_nnn'nTGC
Cuts at: 0 659 1149 1535 1925 4868 5412 6078 6135
Size: 659 490 386 390 2943 544 666 57
Fragments arranged by size:
2943 666 659 544 490 390 386 57
BstEII G'GTnAC_C
Cuts at: 0 2166 5443 6135
Size: 2166 3277 692
Fragments arranged by size:
3277 2166 692
BstXI CCAn_nnnn'nTGG
Cuts at: 0 371 615 3156 5367 5952 6135
Size: 371 244 2541 2211 585 183
Fragments arranged by size:
2541 2211 585 371 244 183
BstYI r'GATC_y
Cuts at: 0 158 338 1574 3677 6135
Size: 158 180 1236 2103 2458
Fragments arranged by size:
2458 2103 1236 180 158
Bsu36I CC'TnA_GG
Cuts at: 0 2461 4214 4546 5463 6135
Size: 2461 1753 332 917 672
Fragments arranged by size:
2461 1753 917 672 332
BtgI C'CryG_G
Cuts at: 0 714 828 1224 1550 2391 2663 3128 3806
Size: 714 114 396 326 841 272 465 678
Cuts at: 3806 3902 4017 4511 4674 4791 5735 5813 5999
Size: 96 115 494 163 117 944 78 186
Cuts at: 5999 6135
Size: 136
Fragments arranged by size:
944 841 714 678 494 465 396 326
272 186 163 136 117 115 114 96
78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2841 3047 3215 3812 6135
Size: 2841 206 168 597 2323
Fragments arranged by size:
2841 2323 597 206 168
BtsI GCAGTG_nn'
Cuts at: 0 685 1420 1621 1855 1926 2641 2986 3077
Size: 685 735 201 234 71 715 345 91
Cuts at: 3077 3821 3935 5080 5550 5659 6010 6135
Size: 744 114 1145 470 109 351 125
Fragments arranged by size:
1145 744 735 715 685 470 351 345
234 201 125 114 109 91 71
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2790 2825 6135
Size: 2790 35 3310
Fragments arranged by size:
3310 2790 35
DraIII CAC_nnn'GTG
Cuts at: 0 2183 3104 6135
Size: 2183 921 3031
Fragments arranged by size:
3031 2183 921
DrdI GACnn_nn'nnGTC
Cuts at: 0 1081 6135
Size: 1081 5054
EaeI y'GGCC_r
Cuts at: 0 2 6 31 56 373 785 1158 1227
Size: 2 4 25 25 317 412 373 69
Cuts at: 1227 2517 2646 3278 3715 3809 4281 4734 5196
Size: 1290 129 632 437 94 472 453 462
Cuts at: 5196 5762 6135
Size: 566 373
Fragments arranged by size:
1290 632 566 472 462 453 437 412
373 373 317 129 94 69 25 25
4 2
EagI C'GGCC_G
Cuts at: 0 6 31 785 2646 3715 4734 5196 6135
Size: 6 25 754 1861 1069 1019 462 939
Fragments arranged by size:
1861 1069 1019 939 754 462 25 6
EarI CTCTTCn'nnn_
Cuts at: 0 589 3532 4759 6135
Size: 589 2943 1227 1376
Fragments arranged by size:
2943 1376 1227 589
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 691 3044 3153 5628 6135
Size: 691 2353 109 2475 507
Fragments arranged by size:
2475 2353 691 507 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 390 457 470 1564 1936 2103 2212
Size: 127 263 67 13 1094 372 167 109
Cuts at: 2212 2869 3433 3439 3624 4228 4390 4540 4609
Size: 657 564 6 185 604 162 150 69
Cuts at: 4609 4720 4957 5011 5119 5206 5295 5296 5742
Size: 111 237 54 108 87 89 1 446
Cuts at: 5742 6109 6135
Size: 367 26
Fragments arranged by size:
1094 657 604 564 446 372 367 263
237 185 167 162 150 127 111 109
108 89 87 69 67 54 26 13
6 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 439 1636 2980 5119 5172 6088 6135
Size: 439 1197 1344 2139 53 916 47
Fragments arranged by size:
2139 1344 1197 916 439 53 47
EcoO109I rG'GnC_Cy
Cuts at: 0 473 735 1268 1374 1389 1594 2254 2457
Size: 473 262 533 106 15 205 660 203
Cuts at: 2457 2659 2871 3576 3583 3663 3861 4025 4682
Size: 202 212 705 7 80 198 164 657
Cuts at: 4682 4683 4901 4968 5014 5486 5913 5982 6135
Size: 1 218 67 46 472 427 69 153
Fragments arranged by size:
705 660 657 533 473 472 427 262
218 212 205 203 202 198 164 153
106 80 69 67 46 15 7 1
EcoRI G'AATT_C
Cuts at: 0 3534 4195 6135
Size: 3534 661 1940
Fragments arranged by size:
3534 1940 661
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 5082 6135
Size: 5082 1053
FseI GG_CCGG'CC
Cuts at: 0 8 6135
Size: 8 6127
FspI TGC'GCA
Cuts at: 0 5692 6135
Size: 5692 443
HaeII r_GCGC'y
Cuts at: 0 542 622 696 836 869 968 1017 1063
Size: 542 80 74 140 33 99 49 46
Cuts at: 1063 1656 2561 3008 3696 4091 4115 4207 4273
Size: 593 905 447 688 395 24 92 66
Cuts at: 4273 4392 4519 4541 4587 4804 4990 5303 5945
Size: 119 127 22 46 217 186 313 642
Cuts at: 5945 6135
Size: 190
Fragments arranged by size:
905 688 642 593 542 447 395 313
217 190 186 140 127 119 99 92
80 74 66 49 46 46 33 24
22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 727 759 2074 2106 2492 2524 3036 3068
Size: 727 32 1315 32 386 32 512 32
Cuts at: 3068 3457 3489 4076 4108 5056 5088 5416 5448
Size: 389 32 587 32 948 32 328 32
Cuts at: 5448 6135
Size: 687
Fragments arranged by size:
1315 948 727 687 587 512 389 386
328 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 4575 5841 6135
Size: 4575 1266 294
Fragments arranged by size:
4575 1266 294
KasI G'GCGC_C
Cuts at: 0 538 618 832 865 964 1013 1059 2557
Size: 538 80 214 33 99 49 46 1498
Cuts at: 2557 3004 3692 4087 4111 4388 4537 4800 5941
Size: 447 688 395 24 277 149 263 1141
Cuts at: 5941 6135
Size: 194
Fragments arranged by size:
1498 1141 688 538 447 395 277 263
214 194 149 99 80 49 46 33
24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 614 770 1730 3401 4722 6135
Size: 520 94 156 960 1671 1321 1413
Fragments arranged by size:
1671 1413 1321 960 520 156 94
MscI TGG'CCA
Cuts at: 0 375 3280 5764 6135
Size: 375 2905 2484 371
Fragments arranged by size:
2905 2484 375 371
MslI CAynn'nnrTG
Cuts at: 0 369 487 3394 6135
Size: 369 118 2907 2741
Fragments arranged by size:
2907 2741 369 118
MspA1I CmG'CkG
Cuts at: 0 27 36 284 672 716 989 1238 1607
Size: 27 9 248 388 44 273 249 369
Cuts at: 1607 1786 2123 2271 2474 2543 2665 2847 3720
Size: 179 337 148 203 69 122 182 873
Cuts at: 3720 4067 5021 5131 5367 5391 5581 5852 5937
Size: 347 954 110 236 24 190 271 85
Cuts at: 5937 5952 6078 6135
Size: 15 126 57
Fragments arranged by size:
954 873 388 369 347 337 273 271
249 248 236 203 190 182 179 148
126 122 110 85 69 57 44 27
24 15 9
NaeI GCC'GGC
Cuts at: 0 6 81 1342 2062 2993 4124 4319 4542
Size: 6 75 1261 720 931 1131 195 223
Cuts at: 4542 6070 6135
Size: 1528 65
Fragments arranged by size:
1528 1261 1131 931 720 223 195 75
65 6
NarI GG'CG_CC
Cuts at: 0 539 619 833 866 965 1014 1060 2558
Size: 539 80 214 33 99 49 46 1498
Cuts at: 2558 3005 3693 4088 4112 4389 4538 4801 5942
Size: 447 688 395 24 277 149 263 1141
Cuts at: 5942 6135
Size: 193
Fragments arranged by size:
1498 1141 688 539 447 395 277 263
214 193 149 99 80 49 46 33
24
NcoI C'CATG_G
Cuts at: 0 2391 4674 4791 6135
Size: 2391 2283 117 1344
Fragments arranged by size:
2391 2283 1344 117
NgoMIV G'CCGG_C
Cuts at: 0 4 79 1340 2060 2991 4122 4317 4540
Size: 4 75 1261 720 931 1131 195 223
Cuts at: 4540 6068 6135
Size: 1528 67
Fragments arranged by size:
1528 1261 1131 931 720 223 195 75
67 4
NotI GC'GGCC_GC
Cuts at: 0 31 3715 6135
Size: 31 3684 2420
Fragments arranged by size:
3684 2420 31
NruI TCG'CGA
Cuts at: 0 3066 6135
Size: 3066 3069
NspI r_CATG'y
Cuts at: 0 92 1737 6135
Size: 92 1645 4398
Fragments arranged by size:
4398 1645 92
PasI CC'CwG_GG
Cuts at: 0 2238 2655 2965 3286 4848 5160 6135
Size: 2238 417 310 321 1562 312 975
Fragments arranged by size:
2238 1562 975 417 321 312 310
PflMI CCAn_nnn'nTGG
Cuts at: 0 1069 2895 3104 4587 4698 5814 5852 6135
Size: 1069 1826 209 1483 111 1116 38 283
Fragments arranged by size:
1826 1483 1116 1069 283 209 111 38
PfoI T'CCnGG_A
Cuts at: 0 3410 6135
Size: 3410 2725
PmlI CAC'GTG
Cuts at: 0 1743 1776 3732 5409 6135
Size: 1743 33 1956 1677 726
Fragments arranged by size:
1956 1743 1677 726 33
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 4984 5016 5874 5906 6135
Size: 433 32 4519 32 858 32 229
Fragments arranged by size:
4519 858 433 229 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 473 735 1594 3576 4025 4968 6135
Size: 473 262 859 1982 449 943 1167
Fragments arranged by size:
1982 1167 943 859 473 449 262
PshAI GACnn'nnGTC
Cuts at: 0 956 5176 5620 6135
Size: 956 4220 444 515
Fragments arranged by size:
4220 956 515 444
PspOMI G'GGCC_C
Cuts at: 0 21 1374 2658 2898 3583 3662 3860 4682
Size: 21 1353 1284 240 685 79 198 822
Cuts at: 4682 4900 6003 6135
Size: 218 1103 132
Fragments arranged by size:
1353 1284 1103 822 685 240 218 198
132 79 21
PspXI vC'TCGA_Gb
Cuts at: 0 1482 3253 6135
Size: 1482 1771 2882
Fragments arranged by size:
2882 1771 1482
PstI C_TGCA'G
Cuts at: 0 671 760 1145 1475 2542 3725 4066 5666
Size: 671 89 385 330 1067 1183 341 1600
Cuts at: 5666 5900 6135
Size: 234 235
Fragments arranged by size:
1600 1183 1067 671 385 341 330 235
234 89
PvuI CG_AT'CG
Cuts at: 0 1117 6135
Size: 1117 5018
PvuII CAG'CTG
Cuts at: 0 989 1786 2123 2543 2847 4067 5367 5391
Size: 989 797 337 420 304 1220 1300 24
Cuts at: 5391 5952 6135
Size: 561 183
Fragments arranged by size:
1300 1220 989 797 561 420 337 304
183 24
SacII CC_GC'GG
Cuts at: 0 717 2666 6135
Size: 717 1949 3469
Fragments arranged by size:
3469 1949 717
SanDI GG'GwC_CC
Cuts at: 0 473 735 6135
Size: 473 262 5400
Fragments arranged by size:
5400 473 262
SapI GCTCTTCn'nnn_
Cuts at: 0 589 4759 6135
Size: 589 4170 1376
Fragments arranged by size:
4170 1376 589
SbfI CC_TGCA'GG
Cuts at: 0 1475 5900 6135
Size: 1475 4425 235
Fragments arranged by size:
4425 1475 235
SexAI A'CCwGG_T
Cuts at: 0 233 314 6135
Size: 233 81 5821
Fragments arranged by size:
5821 233 81
SfcI C'TryA_G
Cuts at: 0 648 667 756 1141 1272 1471 2172 2538
Size: 648 19 89 385 131 199 701 366
Cuts at: 2538 3721 4062 5662 5896 6135
Size: 1183 341 1600 234 239
Fragments arranged by size:
1600 1183 701 648 385 366 341 239
234 199 131 89 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 5021 6135
Size: 5021 1114
SfoI GGC'GCC
Cuts at: 0 540 620 834 867 966 1015 1061 2559
Size: 540 80 214 33 99 49 46 1498
Cuts at: 2559 3006 3694 4089 4113 4390 4539 4802 5943
Size: 447 688 395 24 277 149 263 1141
Cuts at: 5943 6135
Size: 192
Fragments arranged by size:
1498 1141 688 540 447 395 277 263
214 192 149 99 80 49 46 33
24
SmaI CCC'GGG
Cuts at: 0 20 244 471 1129 1978 2344 2610 3661
Size: 20 224 227 658 849 366 266 1051
Cuts at: 3661 4246 5458 5532 6135
Size: 585 1212 74 603
Fragments arranged by size:
1212 1051 849 658 603 585 366 266
227 224 74 20
SmlI C'TyrA_G
Cuts at: 0 1482 3253 6135
Size: 1482 1771 2882
Fragments arranged by size:
2882 1771 1482
SnaBI TAC'GTA
Cuts at: 0 2229 5292 6135
Size: 2229 3063 843
Fragments arranged by size:
3063 2229 843
SphI G_CATG'C
Cuts at: 0 1737 6135
Size: 1737 4398
SrfI GCCC'GGGC
Cuts at: 0 1978 3661 6135
Size: 1978 1683 2474
Fragments arranged by size:
2474 1978 1683
StuI AGG'CCT
Cuts at: 0 4949 6022 6135
Size: 4949 1073 113
Fragments arranged by size:
4949 1073 113
StyI C'CwwG_G
Cuts at: 0 2391 2553 2801 3260 3392 3701 4674 4791
Size: 2391 162 248 459 132 309 973 117
Cuts at: 4791 5597 6017 6135
Size: 806 420 118
Fragments arranged by size:
2391 973 806 459 420 309 248 162
132 118 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3162 5659 6135
Size: 3162 2497 476
Fragments arranged by size:
3162 2497 476
TatI w'GTAC_w
Cuts at: 0 2530 4229 6135
Size: 2530 1699 1906
Fragments arranged by size:
2530 1906 1699
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 175 207 1799 1831 2089 2121 4181 4213
Size: 175 32 1592 32 258 32 2060 32
Cuts at: 4213 6135
Size: 1922
Fragments arranged by size:
2060 1922 1592 258 175 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1081 1102 1252 1516 1906 6097 6135
Size: 1081 21 150 264 390 4191 38
Fragments arranged by size:
4191 1081 390 264 150 38 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 322 1696 3229 5181 5709 6135
Size: 322 1374 1533 1952 528 426
Fragments arranged by size:
1952 1533 1374 528 426 322
XhoI C'TCGA_G
Cuts at: 0 1482 3253 6135
Size: 1482 1771 2882
Fragments arranged by size:
2882 1771 1482
XmaI C'CCGG_G
Cuts at: 0 18 242 469 1127 1976 2342 2608 3659
Size: 18 224 227 658 849 366 266 1051
Cuts at: 3659 4244 5456 5530 6135
Size: 585 1212 74 605
Fragments arranged by size:
1212 1051 849 658 605 585 366 266
227 224 74 18
XmnI GAAnn'nnTTC
Cuts at: 0 925 3553 6135
Size: 925 2628 2582
Fragments arranged by size:
2628 2582 925
ZraI GAC'GTC
Cuts at: 0 3543 3961 4572 5427 6135
Size: 3543 418 611 855 708
Fragments arranged by size:
3543 855 708 611 418
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NruI NspI PasI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII SacII SanDI SapI SbfI SexAI SfcI
SfiI SfoI SmaI SmlI SnaBI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AscI AseI
AsiSI AvrII BaeI BamHI BfrBI BglII BmtI BpuEI
BsaBI BsiWI BsmBI BspHI BstBI BstZ17I ClaI DraI
EcoICRI EcoRV FspAI HindIII HpaI KpnI MfeI MluI
NdeI NheI NsiI PacI PciI PmeI PsiI PsrI
RsrII SacI SalI ScaI SgfI SgrAI SpeI SspI
SwaI XbaI