Restriction map for
pF1KB9481
(Linear) (Six-base) MAPSORT of: KIBB9481.seq Check: 9019 from: 1 to: 6645
KIBB9481.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 0 673 1707 3191 6515 6645
Size: 673 1034 1484 3324 130
Fragments arranged by size:
3324 1484 1034 673 130
Acc65I G'GTAC_C
Cuts at: 0 4913 6645
Size: 4913 1732
AclI AA'CG_TT
Cuts at: 0 1224 6035 6645
Size: 1224 4811 610
Fragments arranged by size:
4811 1224 610
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 722 1558 2276 2733 2808 3622 3911
Size: 27 695 836 718 457 75 814 289
Cuts at: 3911 5176 5386 5449 5940 6645
Size: 1265 210 63 491 705
Fragments arranged by size:
1265 836 814 718 705 695 491 457
289 210 75 63 27
AflIII A'CryG_T
Cuts at: 0 3536 6645
Size: 3536 3109
AhdI GACnn_n'nnGTC
Cuts at: 0 2360 6503 6645
Size: 2360 4143 142
Fragments arranged by size:
4143 2360 142
AleI CACnn'nnGTG
Cuts at: 0 5018 5122 5881 6355 6365 6645
Size: 5018 104 759 474 10 280
Fragments arranged by size:
5018 759 474 280 104 10
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3725 6383 6645
Size: 3725 2658 262
Fragments arranged by size:
3725 2658 262
AlwNI CAG_nnn'CTG
Cuts at: 0 2314 2845 3115 3643 3913 4177 4246 6291
Size: 2314 531 270 528 270 264 69 2045
Cuts at: 6291 6645
Size: 354
Fragments arranged by size:
2314 2045 531 528 354 270 270 264
69
ApaI G_GGCC'C
Cuts at: 0 4505 6350 6645
Size: 4505 1845 295
Fragments arranged by size:
4505 1845 295
ApaLI G'TGCA_C
Cuts at: 0 6358 6460 6645
Size: 6358 102 185
Fragments arranged by size:
6358 185 102
ApoI r'AATT_y
Cuts at: 0 10 791 1127 2013 2022 2209 2283 2401
Size: 10 781 336 886 9 187 74 118
Cuts at: 2401 3308 3805 5347 5434 6645
Size: 907 497 1542 87 1211
Fragments arranged by size:
1542 1211 907 886 781 497 336 187
118 87 74 10 9
AvaI C'yCGr_G
Cuts at: 0 6336 6645
Size: 6336 309
AvrII C'CTAG_G
Cuts at: 0 5316 6645
Size: 5316 1329
BamHI G'GATC_C
Cuts at: 0 1373 1880 4778 6645
Size: 1373 507 2898 1867
Fragments arranged by size:
2898 1867 1373 507
BanI G'GyrC_C
Cuts at: 0 267 2229 2652 3950 4913 5995 6645
Size: 267 1962 423 1298 963 1082 650
Fragments arranged by size:
1962 1298 1082 963 650 423 267
BanII G_rGCy'C
Cuts at: 0 124 802 1967 3989 4044 4505 5498 6350
Size: 124 678 1165 2022 55 461 993 852
Cuts at: 6350 6645
Size: 295
Fragments arranged by size:
2022 1165 993 852 678 461 295 124
55
BbeI G_GCGC'C
Cuts at: 0 2656 6645
Size: 2656 3989
BbsI GAAGACnn'nnnn_
Cuts at: 0 1024 1983 4560 4609 4973 5045 6645
Size: 1024 959 2577 49 364 72 1600
Fragments arranged by size:
2577 1600 1024 959 364 72 49
BbvCI CC'TCA_GC
Cuts at: 0 2632 6645
Size: 2632 4013
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 263 297 452 486 6645
Size: 263 34 155 34 6159
Fragments arranged by size:
6159 263 155 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2913 6645
Size: 2913 3732
BclI T'GATC_A
Cuts at: 0 288 2931 6645
Size: 288 2643 3714
Fragments arranged by size:
3714 2643 288
BfrBI ATG'CAT
Cuts at: 0 3670 6645
Size: 3670 2975
BglII A'GATC_T
Cuts at: 0 3813 4395 6645
Size: 3813 582 2250
Fragments arranged by size:
3813 2250 582
BlpI GC'TnA_GC
Cuts at: 0 240 3988 6645
Size: 240 3748 2657
Fragments arranged by size:
3748 2657 240
Bme1580I G_kGCm'C
Cuts at: 0 859 4505 4904 6350 6362 6464 6645
Size: 859 3646 399 1446 12 102 181
Fragments arranged by size:
3646 1446 859 399 181 102 12
BmgBI CAC'GTC
Cuts at: 0 3525 5790 6645
Size: 3525 2265 855
Fragments arranged by size:
3525 2265 855
BmrI ACTGGGnnnn_n'
Cuts at: 0 1875 4439 6054 6279 6645
Size: 1875 2564 1615 225 366
Fragments arranged by size:
2564 1875 1615 366 225
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5905 6559 6645
Size: 5905 654 86
Fragments arranged by size:
5905 654 86
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 330 675 747 1235 1250 1822 1880
Size: 316 14 345 72 488 15 572 58
Cuts at: 1880 2050 2442 2942 3571 4015 4254 4882 5212
Size: 170 392 500 629 444 239 628 330
Cuts at: 5212 6400 6645
Size: 1188 245
Fragments arranged by size:
1188 629 628 572 500 488 444 392
345 330 316 245 239 170 72 58
15 14
Bpu10I CC'TnA_GC
Cuts at: 0 2632 6645
Size: 2632 4013
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 2476 4028 6645
Size: 325 2151 1552 2617
Fragments arranged by size:
2617 2151 1552 325
BsaI GGTCTCn'nnnn_
Cuts at: 0 1835 2159 2458 6645
Size: 1835 324 299 4187
Fragments arranged by size:
4187 1835 324 299
BsaAI yAC'GTr
Cuts at: 0 4368 4493 6645
Size: 4368 125 2152
Fragments arranged by size:
4368 2152 125
BsaBI GATnn'nnATC
Cuts at: 0 3844 4025 5906 6645
Size: 3844 181 1881 739
Fragments arranged by size:
3844 1881 739 181
BsaHI Gr'CG_yC
Cuts at: 0 2653 6645
Size: 2653 3992
BsaWI w'CCGG_w
Cuts at: 0 1060 2614 5681 6645
Size: 1060 1554 3067 964
Fragments arranged by size:
3067 1554 1060 964
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 452 482 2588 2618 2852 2882 4321 4351
Size: 452 30 2106 30 234 30 1439 30
Cuts at: 4351 5194 5224 5257 5287 6645
Size: 843 30 33 30 1358
Fragments arranged by size:
2106 1439 1358 843 452 234 33 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 492 4991 5791 6645
Size: 492 4499 800 854
Fragments arranged by size:
4499 854 800 492
BseYI C'CCAG_C
Cuts at: 0 3906 4170 6343 6645
Size: 3906 264 2173 302
Fragments arranged by size:
3906 2173 302 264
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 619 4954 6645
Size: 179 440 4335 1691
Fragments arranged by size:
4335 1691 440 179
BsiEI CG_ry'CG
Cuts at: 0 1707 2619 4943 6645
Size: 1707 912 2324 1702
Fragments arranged by size:
2324 1707 1702 912
BsiHKAI G_wGCw'C
Cuts at: 0 484 3148 3536 3989 4044 6362 6464 6645
Size: 484 2664 388 453 55 2318 102 181
Fragments arranged by size:
2664 2318 484 453 388 181 102 55
BsmI GAATG_Cn'
Cuts at: 0 2984 3478 4822 5393 5627 6645
Size: 2984 494 1344 571 234 1018
Fragments arranged by size:
2984 1344 1018 571 494 234
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2387 3446 6645
Size: 2387 1059 3199
Fragments arranged by size:
3199 2387 1059
Bsp1286I G_dGCh'C
Cuts at: 0 124 484 802 859 1967 3148 3536 3989
Size: 124 360 318 57 1108 1181 388 453
Cuts at: 3989 4044 4505 4904 5498 6350 6362 6464 6645
Size: 55 461 399 594 852 12 102 181
Fragments arranged by size:
1181 1108 852 594 461 453 399 388
360 318 181 124 102 57 55 12
BspEI T'CCGG_A
Cuts at: 0 1060 6645
Size: 1060 5585
BspHI T'CATG_A
Cuts at: 0 2691 3575 4889 5729 6645
Size: 2691 884 1314 840 916
Fragments arranged by size:
2691 1314 916 884 840
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 40 289 4742 6645
Size: 40 249 4453 1903
Fragments arranged by size:
4453 1903 249 40
BsrBI CCG'CTC
Cuts at: 0 239 2835 6645
Size: 239 2596 3810
Fragments arranged by size:
3810 2596 239
BsrDI GCAATG_nn'
Cuts at: 0 2720 3130 4466 4737 4763 5813 6645
Size: 2720 410 1336 271 26 1050 832
Fragments arranged by size:
2720 1336 1050 832 410 271 26
BsrFI r'CCGG_y
Cuts at: 0 1030 1349 4742 4942 6645
Size: 1030 319 3393 200 1703
Fragments arranged by size:
3393 1703 1030 319 200
BsrGI T'GTAC_A
Cuts at: 0 3038 3336 4010 4100 6645
Size: 3038 298 674 90 2545
Fragments arranged by size:
3038 2545 674 298 90
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3667 4545 6645
Size: 3667 878 2100
Fragments arranged by size:
3667 2100 878
BstEII G'GTnAC_C
Cuts at: 0 5677 6054 6645
Size: 5677 377 591
Fragments arranged by size:
5677 591 377
BstXI CCAn_nnnn'nTGG
Cuts at: 0 354 1330 4860 4959 6645
Size: 354 976 3530 99 1686
Fragments arranged by size:
3530 1686 976 354 99
BstYI r'GATC_y
Cuts at: 0 978 1373 1880 2540 3123 3203 3813 3845
Size: 978 395 507 660 583 80 610 32
Cuts at: 3845 4395 4630 4699 4778 6624 6645
Size: 550 235 69 79 1846 21
Fragments arranged by size:
1846 978 660 610 583 550 507 395
235 80 79 69 32 21
Bsu36I CC'TnA_GG
Cuts at: 0 3925 6645
Size: 3925 2720
BtgI C'CryG_G
Cuts at: 0 225 1323 1520 2513 4757 5595 6264 6645
Size: 225 1098 197 993 2244 838 669 381
Fragments arranged by size:
2244 1098 993 838 669 381 225 197
BtsI GCAGTG_nn'
Cuts at: 0 157 837 1172 1772 4546 5151 5375 6360
Size: 157 680 335 600 2774 605 224 985
Cuts at: 6360 6372 6464 6645
Size: 12 92 181
Fragments arranged by size:
2774 985 680 605 600 335 224 181
157 92 12
ClaI AT'CG_AT
Cuts at: 0 3273 5847 6645
Size: 3273 2574 798
Fragments arranged by size:
3273 2574 798
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4746 4781 6645
Size: 4746 35 1864
Fragments arranged by size:
4746 1864 35
DraI TTT'AAA
Cuts at: 0 1689 6645
Size: 1689 4956
DrdI GACnn_nn'nnGTC
Cuts at: 0 4057 6268 6645
Size: 4057 2211 377
Fragments arranged by size:
4057 2211 377
EaeI y'GGCC_r
Cuts at: 0 206 2339 4484 4760 4940 6100 6256 6645
Size: 206 2133 2145 276 180 1160 156 389
Fragments arranged by size:
2145 2133 1160 389 276 206 180 156
EagI C'GGCC_G
Cuts at: 0 4940 6645
Size: 4940 1705
EarI CTCTTCn'nnn_
Cuts at: 0 225 698 947 1894 2295 2550 3220 5955
Size: 225 473 249 947 401 255 670 2735
Cuts at: 5955 6645
Size: 690
Fragments arranged by size:
2735 947 690 670 473 401 255 249
225
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 733 763 3642 6223 6645
Size: 733 30 2879 2581 422
Fragments arranged by size:
2879 2581 733 422 30
EcoICRI GAG'CTC
Cuts at: 0 3987 4042 6645
Size: 3987 55 2603
Fragments arranged by size:
3987 2603 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 27 316 330 675 722 747 1235 1250
Size: 27 289 14 345 47 25 488 15
Cuts at: 1250 1558 1822 1880 2050 2276 2442 2733 2808
Size: 308 264 58 170 226 166 291 75
Cuts at: 2808 2942 3571 3622 3911 4015 4254 4882 5176
Size: 134 629 51 289 104 239 628 294
Cuts at: 5176 5212 5386 5449 5940 6400 6645
Size: 36 174 63 491 460 245
Fragments arranged by size:
629 628 491 488 460 345 308 294
291 289 289 264 245 239 226 174
170 166 134 104 75 63 58 51
47 36 27 25 15 14
EcoO109I rG'GnC_Cy
Cuts at: 0 3596 4320 4501 4502 5424 6132 6645
Size: 3596 724 181 1 922 708 513
Fragments arranged by size:
3596 922 724 708 513 181 1
EcoRI G'AATT_C
Cuts at: 0 2022 2209 5434 6645
Size: 2022 187 3225 1211
Fragments arranged by size:
3225 2022 1211 187
EcoRV GAT'ATC
Cuts at: 0 3735 6645
Size: 3735 2910
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 319 3543 6024 6645
Size: 319 3224 2481 621
Fragments arranged by size:
3224 2481 621 319
FspI TGC'GCA
Cuts at: 0 3136 3769 6645
Size: 3136 633 2876
Fragments arranged by size:
3136 2876 633
HaeII r_GCGC'y
Cuts at: 0 2656 6645
Size: 2656 3989
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 341 373 609 641 927 959 1815 1847
Size: 341 32 236 32 286 32 856 32
Cuts at: 1847 2346 2378 3719 3751 4104 4136 4620 4652
Size: 499 32 1341 32 353 32 484 32
Cuts at: 4652 5514 5546 5583 5615 5899 5931 6489 6521
Size: 862 32 37 32 284 32 558 32
Cuts at: 6521 6614 6645
Size: 93 31
Fragments arranged by size:
1341 862 856 558 499 484 353 341
286 284 236 93 37 32 32 32
32 32 32 32 32 32 32 32
32 31
HincII GTy'rAC
Cuts at: 0 405 674 1708 3192 3501 4050 5073 6645
Size: 405 269 1034 1484 309 549 1023 1572
Fragments arranged by size:
1572 1484 1034 1023 549 405 309 269
HindIII A'AGCT_T
Cuts at: 0 5293 6645
Size: 5293 1352
KasI G'GCGC_C
Cuts at: 0 2652 6645
Size: 2652 3993
KpnI G_GTAC'C
Cuts at: 0 4917 6645
Size: 4917 1728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 239 5774 6645
Size: 239 5535 871
Fragments arranged by size:
5535 871 239
MscI TGG'CCA
Cuts at: 0 208 2341 4486 6102 6258 6645
Size: 208 2133 2145 1616 156 387
Fragments arranged by size:
2145 2133 1616 387 208 156
MslI CAynn'nnrTG
Cuts at: 0 273 888 1388 1462 1921 2087 2429 4891
Size: 273 615 500 74 459 166 342 2462
Cuts at: 4891 4930 5018 5020 5122 5218 5551 5881 6049
Size: 39 88 2 102 96 333 330 168
Cuts at: 6049 6355 6365 6527 6645
Size: 306 10 162 118
Fragments arranged by size:
2462 615 500 459 342 333 330 306
273 168 166 162 118 102 96 88
74 39 10 2
MspA1I CmG'CkG
Cuts at: 0 227 257 1207 1730 3210 3593 3913 3965
Size: 227 30 950 523 1480 383 320 52
Cuts at: 3965 3992 4417 5081 6106 6645
Size: 27 425 664 1025 539
Fragments arranged by size:
1480 1025 950 664 539 523 425 383
320 227 52 30 27
NaeI GCC'GGC
Cuts at: 0 4744 4944 6645
Size: 4744 200 1701
Fragments arranged by size:
4744 1701 200
NarI GG'CG_CC
Cuts at: 0 2653 6645
Size: 2653 3992
NcoI C'CATG_G
Cuts at: 0 1323 1520 5595 6264 6645
Size: 1323 197 4075 669 381
Fragments arranged by size:
4075 1323 669 381 197
NdeI CA'TA_TG
Cuts at: 0 1077 6645
Size: 1077 5568
NgoMIV G'CCGG_C
Cuts at: 0 4742 4942 6645
Size: 4742 200 1703
Fragments arranged by size:
4742 1703 200
NsiI A_TGCA'T
Cuts at: 0 3672 6645
Size: 3672 2973
NspI r_CATG'y
Cuts at: 0 1042 1408 2086 2338 2917 3163 5550 6645
Size: 1042 366 678 252 579 246 2387 1095
Fragments arranged by size:
2387 1095 1042 678 579 366 252 246
PasI CC'CwG_GG
Cuts at: 0 840 6645
Size: 840 5805
PflMI CCAn_nnn'nTGG
Cuts at: 0 3549 4246 4493 4696 6265 6645
Size: 3549 697 247 203 1569 380
Fragments arranged by size:
3549 1569 697 380 247 203
PfoI T'CCnGG_A
Cuts at: 0 1593 4031 4353 4669 6182 6645
Size: 1593 2438 322 316 1513 463
Fragments arranged by size:
2438 1593 1513 463 322 316
PmlI CAC'GTG
Cuts at: 0 4493 6645
Size: 4493 2152
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3523 3555 6645
Size: 3523 32 3090
Fragments arranged by size:
3523 3090 32
PpuMI rG'GwC_Cy
Cuts at: 0 3596 4320 5424 6132 6645
Size: 3596 724 1104 708 513
Fragments arranged by size:
3596 1104 724 708 513
PspOMI G'GGCC_C
Cuts at: 0 4501 6346 6645
Size: 4501 1845 299
Fragments arranged by size:
4501 1845 299
PstI C_TGCA'G
Cuts at: 0 591 1479 4541 5080 5581 6146 6367 6645
Size: 591 888 3062 539 501 565 221 278
Fragments arranged by size:
3062 888 591 565 539 501 278 221
PvuII CAG'CTG
Cuts at: 0 1207 1730 3210 3913 3992 5081 6645
Size: 1207 523 1480 703 79 1089 1564
Fragments arranged by size:
1564 1480 1207 1089 703 523 79
SacI G_AGCT'C
Cuts at: 0 3989 4044 6645
Size: 3989 55 2601
Fragments arranged by size:
3989 2601 55
SacII CC_GC'GG
Cuts at: 0 228 6645
Size: 228 6417
SalI G'TCGA_C
Cuts at: 0 672 1706 3190 6645
Size: 672 1034 1484 3455
Fragments arranged by size:
3455 1484 1034 672
SanDI GG'GwC_CC
Cuts at: 0 3596 6645
Size: 3596 3049
SapI GCTCTTCn'nnn_
Cuts at: 0 3220 6645
Size: 3220 3425
ScaI AGT'ACT
Cuts at: 0 1140 6645
Size: 1140 5505
SexAI A'CCwGG_T
Cuts at: 0 2225 4916 6645
Size: 2225 2691 1729
Fragments arranged by size:
2691 2225 1729
SfcI C'TryA_G
Cuts at: 0 587 597 825 1475 1491 2123 2252 4537
Size: 587 10 228 650 16 632 129 2285
Cuts at: 4537 5076 5382 5577 5737 6142 6363 6645
Size: 539 306 195 160 405 221 282
Fragments arranged by size:
2285 650 632 587 539 405 306 282
228 221 195 160 129 16 10
SfoI GGC'GCC
Cuts at: 0 2654 6645
Size: 2654 3991
SmaI CCC'GGG
Cuts at: 0 6338 6645
Size: 6338 307
SmlI C'TyrA_G
Cuts at: 0 304 2491 4043 6645
Size: 304 2187 1552 2602
Fragments arranged by size:
2602 2187 1552 304
SpeI A'CTAG_T
Cuts at: 0 1503 4056 6645
Size: 1503 2553 2589
Fragments arranged by size:
2589 2553 1503
SphI G_CATG'C
Cuts at: 0 2917 5550 6645
Size: 2917 2633 1095
Fragments arranged by size:
2917 2633 1095
SspI AAT'ATT
Cuts at: 0 1194 3390 6645
Size: 1194 2196 3255
Fragments arranged by size:
3255 2196 1194
StuI AGG'CCT
Cuts at: 0 571 760 3866 4346 6645
Size: 571 189 3106 480 2299
Fragments arranged by size:
3106 2299 571 480 189
StyI C'CwwG_G
Cuts at: 0 755 1323 1520 1666 2037 2346 3648 4481
Size: 755 568 197 146 371 309 1302 833
Cuts at: 4481 5316 5595 5765 5999 6264 6645
Size: 835 279 170 234 265 381
Fragments arranged by size:
1302 835 833 755 568 381 371 309
279 265 234 197 170 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2898 4365 4849 6298 6422 6645
Size: 2898 1467 484 1449 124 223
Fragments arranged by size:
2898 1467 1449 484 223 124
TatI w'GTAC_w
Cuts at: 0 1138 2507 3038 3336 4010 4100 5195 6645
Size: 1138 1369 531 298 674 90 1095 1450
Fragments arranged by size:
1450 1369 1138 1095 674 531 298 90
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1241 1273 2294 2326 2588 2620 3429 3461
Size: 1241 32 1021 32 262 32 809 32
Cuts at: 3461 4592 4624 5194 5226 6645
Size: 1131 32 570 32 1419
Fragments arranged by size:
1419 1241 1131 1021 809 570 262 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2851 3168 6645
Size: 2851 317 3477
Fragments arranged by size:
3477 2851 317
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 6438 6645
Size: 6438 207
XmaI C'CCGG_G
Cuts at: 0 6336 6645
Size: 6336 309
XmnI GAAnn'nnTTC
Cuts at: 0 59 1224 5113 5434 6645
Size: 59 1165 3889 321 1211
Fragments arranged by size:
3889 1211 1165 321 59
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII
SalI SanDI SapI ScaI SexAI SfcI SfoI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AloI AscI AseI
AsiSI BaeI BglI BmtI BsiWI BssHII BssSI BstBI
BstZ17I BtgZI DraIII EcoNI FseI FspAI HpaI MfeI
MluI NheI NotI NruI PacI PciI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SbfI SfiI SgfI
SgrAI SnaBI SrfI SwaI XbaI XhoI ZraI