Restriction map for
pF1KB9478
(Linear) (Six-base) MAPSORT of: KIBB9478.seq Check: 5433 from: 1 to: 3633
KIBB9478.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AccI GT'mk_AC
Cuts at: 0 3053 3633
Size: 3053 580
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1099 1264 3633
Size: 1033 66 165 2369
Fragments arranged by size:
2369 1033 165 66
AflII C'TTAA_G
Cuts at: 0 2644 3633
Size: 2644 989
AflIII A'CryG_T
Cuts at: 0 176 3633
Size: 176 3457
AleI CACnn'nnGTG
Cuts at: 0 326 3538 3633
Size: 326 3212 95
Fragments arranged by size:
3212 326 95
AlwNI CAG_nnn'CTG
Cuts at: 0 474 3019 3064 3633
Size: 474 2545 45 569
Fragments arranged by size:
2545 569 474 45
ApaI G_GGCC'C
Cuts at: 0 1594 1719 2134 3633
Size: 1594 125 415 1499
Fragments arranged by size:
1594 1499 415 125
ApaLI G'TGCA_C
Cuts at: 0 3543 3580 3633
Size: 3543 37 53
Fragments arranged by size:
3543 53 37
ApoI r'AATT_y
Cuts at: 0 1239 3079 3514 3633
Size: 1239 1840 435 119
Fragments arranged by size:
1840 1239 435 119
AvaI C'yCGr_G
Cuts at: 0 1712 1838 1901 1952 2329 3633
Size: 1712 126 63 51 377 1304
Fragments arranged by size:
1712 1304 377 126 63 51
BanI G'GyrC_C
Cuts at: 0 1619 3267 3633
Size: 1619 1648 366
Fragments arranged by size:
1648 1619 366
BanII G_rGCy'C
Cuts at: 0 131 1490 1594 1610 1719 1781 2023 2134
Size: 131 1359 104 16 109 62 242 111
Cuts at: 2134 2410 2767 3633
Size: 276 357 866
Fragments arranged by size:
1359 866 357 276 242 131 111 109
104 62 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 1277 1942 3633
Size: 1277 665 1691
Fragments arranged by size:
1691 1277 665
BciVI GTATCCnnnnn_n'
Cuts at: 0 2309 3633
Size: 2309 1324
BfrBI ATG'CAT
Cuts at: 0 3152 3281 3633
Size: 3152 129 352
Fragments arranged by size:
3152 352 129
BglI GCCn_nnn'nGGC
Cuts at: 0 1614 1714 2559 3633
Size: 1614 100 845 1074
Fragments arranged by size:
1614 1074 845 100
BglII A'GATC_T
Cuts at: 0 3069 3633
Size: 3069 564
BlpI GC'TnA_GC
Cuts at: 0 1139 3529 3633
Size: 1139 2390 104
Fragments arranged by size:
2390 1139 104
Bme1580I G_kGCm'C
Cuts at: 0 1594 1719 1991 2134 3547 3584 3633
Size: 1594 125 272 143 1413 37 49
Fragments arranged by size:
1594 1413 272 143 125 49 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 3 613 3345 3400 3633
Size: 3 610 2732 55 233
Fragments arranged by size:
2732 610 233 55 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 1683 1980 2506 2541 2751 3633
Size: 563 1120 297 526 35 210 882
Fragments arranged by size:
1120 882 563 526 297 210 35
Bpu10I CC'TnA_GC
Cuts at: 0 1514 2093 2254 3633
Size: 1514 579 161 1379
Fragments arranged by size:
1514 1379 579 161
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 2403 2849 3633
Size: 761 1642 446 784
Fragments arranged by size:
1642 784 761 446
BsaI GGTCTCn'nnnn_
Cuts at: 0 542 763 1898 3633
Size: 542 221 1135 1735
Fragments arranged by size:
1735 1135 542 221
BsaWI w'CCGG_w
Cuts at: 0 459 1506 2797 3633
Size: 459 1047 1291 836
Fragments arranged by size:
1291 1047 836 459
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1488 1518 2005 2035 3633
Size: 1488 30 487 30 1598
Fragments arranged by size:
1598 1488 487 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1326 1761 2255 2418 2556 2766 3633
Size: 1326 435 494 163 138 210 867
Fragments arranged by size:
1326 867 494 435 210 163 138
BseYI C'CCAG_C
Cuts at: 0 723 800 1500 2321 3633
Size: 723 77 700 821 1312
Fragments arranged by size:
1312 821 723 700 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1328 1424 2624 3633
Size: 1328 96 1200 1009
Fragments arranged by size:
1328 1200 1009 96
BsiHKAI G_wGCw'C
Cuts at: 0 1359 1603 2023 2099 2410 2786 3547 3584
Size: 1359 244 420 76 311 376 761 37
Cuts at: 3584 3633
Size: 49
Fragments arranged by size:
1359 761 420 376 311 244 76 49
37
BsmI GAATG_Cn'
Cuts at: 0 302 1665 3633
Size: 302 1363 1968
Fragments arranged by size:
1968 1363 302
Bsp1286I G_dGCh'C
Cuts at: 0 131 1359 1490 1594 1603 1610 1719 1781
Size: 131 1228 131 104 9 7 109 62
Cuts at: 1781 1991 2023 2099 2134 2410 2767 2786 3547
Size: 210 32 76 35 276 357 19 761
Cuts at: 3547 3584 3633
Size: 37 49
Fragments arranged by size:
1228 761 357 276 210 131 131 109
104 76 62 49 37 35 32 19
9 7
BspEI T'CCGG_A
Cuts at: 0 1506 2797 3633
Size: 1506 1291 836
Fragments arranged by size:
1506 1291 836
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 47 1834 2312 3633
Size: 47 1787 478 1321
Fragments arranged by size:
1787 1321 478 47
BsrBI CCG'CTC
Cuts at: 0 2253 3633
Size: 2253 1380
BsrDI GCAATG_nn'
Cuts at: 0 860 2218 3281 3633
Size: 860 1358 1063 352
Fragments arranged by size:
1358 1063 860 352
BsrGI T'GTAC_A
Cuts at: 0 20 3633
Size: 20 3613
BssSI C'ACGA_G
Cuts at: 0 1653 3498 3633
Size: 1653 1845 135
Fragments arranged by size:
1845 1653 135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3158 3633
Size: 3158 475
BstBI TT'CG_AA
Cuts at: 0 45 3633
Size: 45 3588
BstEII G'GTnAC_C
Cuts at: 0 529 3633
Size: 529 3104
BstYI r'GATC_y
Cuts at: 0 1680 3069 3633
Size: 1680 1389 564
Fragments arranged by size:
1680 1389 564
BtgI C'CryG_G
Cuts at: 0 869 887 1612 2180 3633
Size: 869 18 725 568 1453
Fragments arranged by size:
1453 869 725 568 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 217 3196 3633
Size: 217 2979 437
Fragments arranged by size:
2979 437 217
BtsI GCAGTG_nn'
Cuts at: 0 755 934 1407 1991 3372 3384 3633
Size: 755 179 473 584 1381 12 249
Fragments arranged by size:
1381 755 584 473 249 179 12
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2261 2296 3633
Size: 2261 35 1337
Fragments arranged by size:
2261 1337 35
EaeI y'GGCC_r
Cuts at: 0 1099 2429 2676 3633
Size: 1099 1330 247 957
Fragments arranged by size:
1330 1099 957 247
EarI CTCTTCn'nnn_
Cuts at: 0 531 665 1064 2395 2733 2742 3633
Size: 531 134 399 1331 338 9 891
Fragments arranged by size:
1331 891 531 399 338 134 9
EcoICRI GAG'CTC
Cuts at: 0 2021 2408 3633
Size: 2021 387 1225
Fragments arranged by size:
2021 1225 387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 1033 1099 1264 1683 1980 2506 2541
Size: 563 470 66 165 419 297 526 35
Cuts at: 2541 2751 3633
Size: 210 882
Fragments arranged by size:
882 563 526 470 419 297 210 165
66 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 290 478 493 3633
Size: 290 188 15 3140
Fragments arranged by size:
3140 290 188 15
EcoO109I rG'GnC_Cy
Cuts at: 0 741 906 1590 1591 1716 2087 2130 2561
Size: 741 165 684 1 125 371 43 431
Cuts at: 2561 2682 3347 3492 3633
Size: 121 665 145 141
Fragments arranged by size:
741 684 665 431 371 165 145 141
125 121 43 1
EcoRI G'AATT_C
Cuts at: 0 3514 3633
Size: 3514 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1294 2911 3633
Size: 1294 1617 722
Fragments arranged by size:
1617 1294 722
HaeII r_GCGC'y
Cuts at: 0 3121 3633
Size: 3121 512
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 142 174 468 500 1008 1040 1423 1455
Size: 142 32 294 32 508 32 383 32
Cuts at: 1455 1737 1769 2293 2325 3633
Size: 282 32 524 32 1308
Fragments arranged by size:
1308 524 508 383 294 282 142 32
32 32 32 32 32
HindIII A'AGCT_T
Cuts at: 0 2277 3633
Size: 2277 1356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1546 1779 2934 3633
Size: 671 875 233 1155 699
Fragments arranged by size:
1155 875 699 671 233
MscI TGG'CCA
Cuts at: 0 2431 2678 3633
Size: 2431 247 955
Fragments arranged by size:
2431 955 247
MslI CAynn'nnrTG
Cuts at: 0 326 3181 3538 3633
Size: 326 2855 357 95
Fragments arranged by size:
2855 357 326 95
MspA1I CmG'CkG
Cuts at: 0 1138 1542 1576 2150 3633
Size: 1138 404 34 574 1483
Fragments arranged by size:
1483 1138 574 404 34
NcoI C'CATG_G
Cuts at: 0 887 3633
Size: 887 2746
NsiI A_TGCA'T
Cuts at: 0 3154 3283 3633
Size: 3154 129 350
Fragments arranged by size:
3154 350 129
NspI r_CATG'y
Cuts at: 0 180 1183 3156 3633
Size: 180 1003 1973 477
Fragments arranged by size:
1973 1003 477 180
PciI A'CATG_T
Cuts at: 0 176 3633
Size: 176 3457
PflMI CCAn_nnn'nTGG
Cuts at: 0 2181 3633
Size: 2181 1452
PfoI T'CCnGG_A
Cuts at: 0 2189 3633
Size: 2189 1444
PpuMI rG'GwC_Cy
Cuts at: 0 741 2087 2682 3347 3633
Size: 741 1346 595 665 286
Fragments arranged by size:
1346 741 665 595 286
PshAI GACnn'nnGTC
Cuts at: 0 1561 3633
Size: 1561 2072
PspOMI G'GGCC_C
Cuts at: 0 1590 1715 2130 3633
Size: 1590 125 415 1503
Fragments arranged by size:
1590 1503 415 125
PstI C_TGCA'G
Cuts at: 0 1865 3633
Size: 1865 1768
PvuII CAG'CTG
Cuts at: 0 1138 1542 1576 3633
Size: 1138 404 34 2057
Fragments arranged by size:
2057 1138 404 34
SacI G_AGCT'C
Cuts at: 0 2023 2410 3633
Size: 2023 387 1223
Fragments arranged by size:
2023 1223 387
SanDI GG'GwC_CC
Cuts at: 0 3347 3633
Size: 3347 286
SapI GCTCTTCn'nnn_
Cuts at: 0 531 3633
Size: 531 3102
SbfI CC_TGCA'GG
Cuts at: 0 1865 3633
Size: 1865 1768
ScaI AGT'ACT
Cuts at: 0 216 3633
Size: 216 3417
SexAI A'CCwGG_T
Cuts at: 0 3575 3633
Size: 3575 58
SfcI C'TryA_G
Cuts at: 0 1861 3065 3633
Size: 1861 1204 568
Fragments arranged by size:
1861 1204 568
SmaI CCC'GGG
Cuts at: 0 1714 1840 1954 2331 3633
Size: 1714 126 114 377 1302
Fragments arranged by size:
1714 1302 377 126 114
SmlI C'TyrA_G
Cuts at: 0 776 2418 2644 2864 3633
Size: 776 1642 226 220 769
Fragments arranged by size:
1642 776 769 226 220
SpeI A'CTAG_T
Cuts at: 0 325 2161 3633
Size: 325 1836 1472
Fragments arranged by size:
1836 1472 325
SphI G_CATG'C
Cuts at: 0 3156 3633
Size: 3156 477
SrfI GCCC'GGGC
Cuts at: 0 1840 3633
Size: 1840 1793
SspI AAT'ATT
Cuts at: 0 3137 3633
Size: 3137 496
StuI AGG'CCT
Cuts at: 0 1831 3633
Size: 1831 1802
StyI C'CwwG_G
Cuts at: 0 489 737 887 1928 3548 3633
Size: 489 248 150 1041 1620 85
Fragments arranged by size:
1620 1041 489 248 150 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 548 1637 3633
Size: 548 1089 1996
Fragments arranged by size:
1996 1089 548
TatI w'GTAC_w
Cuts at: 0 20 214 1132 3633
Size: 20 194 918 2501
Fragments arranged by size:
2501 918 194 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 325 357 2005 2037 3633
Size: 325 32 1648 32 1596
Fragments arranged by size:
1648 1596 325 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 487 3174 3358 3633
Size: 487 2687 184 275
Fragments arranged by size:
2687 487 275 184
XmaI C'CCGG_G
Cuts at: 0 1712 1838 1952 2329 3633
Size: 1712 126 114 377 1304
Fragments arranged by size:
1712 1304 377 126 114
XmnI GAAnn'nnTTC
Cuts at: 0 110 205 1073 3633
Size: 110 95 868 2560
Fragments arranged by size:
2560 868 110 95
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbsI BciVI BfrBI BglI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI CspCI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HindIII MmeI MscI MslI MspA1I NcoI NsiI NspI
PciI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII
SacI SanDI SapI SbfI ScaI SexAI SfcI SmaI
SmlI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BclI BmgBI BmtI BplI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BspHI BsrFI BssHII BstXI
BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI EciI
EcoRV FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacII SalI SfiI SfoI SgfI
SgrAI SnaBI SwaI Tth111I XbaI XhoI ZraI