(Linear) (Six-base) MAPSORT of: KIBB9474.seq  Check: 6517  from: 1  to: 582



KIBB9474.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:39  ..

AgeI A'CCGG_T

Cuts at:      0     232     582 
   Size:        232     350
 
ApoI r'AATT_y

Cuts at:      0       9     582 
   Size:          9     573
 
AvaI C'yCGr_G

Cuts at:      0     267     582 
   Size:        267     315
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     331     582 
   Size:        331     251
 
BglI GCCn_nnn'nGGC

Cuts at:      0      28     109     565     582 
   Size:         28      81     456      17

  Fragments arranged by size: 

                 456      81      28      17
 
BmgBI CAC'GTC

Cuts at:      0      17     582 
   Size:         17     565
 
BmtI G_CTAG'C

Cuts at:      0     286     582 
   Size:        286     296
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     582 
   Size:        226     356
 
BsaWI w'CCGG_w

Cuts at:      0     232     582 
   Size:        232     350
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     123     582 
   Size:        123     459
 
BsrFI r'CCGG_y

Cuts at:      0     232     498     568     582 
   Size:        232     266      70      14

  Fragments arranged by size: 

                 266     232      70      14
 
BstEII G'GTnAC_C

Cuts at:      0     225     582 
   Size:        225     357
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     258     582 
   Size:        258     324
 
EaeI y'GGCC_r

Cuts at:      0      29     582 
   Size:         29     553
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0       9     582 
   Size:          9     573
 
EcoO109I rG'GnC_Cy

Cuts at:      0     263     272     582 
   Size:        263       9     310

  Fragments arranged by size: 

                 310     263       9
 
EcoRI G'AATT_C

Cuts at:      0       9     582 
   Size:          9     573
 
HaeII r_GCGC'y

Cuts at:      0     222     582 
   Size:        222     360
 
MslI CAynn'nnrTG

Cuts at:      0      94     582 
   Size:         94     488
 
NaeI GCC'GGC

Cuts at:      0     570     582 
   Size:        570      12
 
NgoMIV G'CCGG_C

Cuts at:      0     568     582 
   Size:        568      14
 
NheI G'CTAG_C

Cuts at:      0     282     582 
   Size:        282     300
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     178     210     582 
   Size:        178      32     372

  Fragments arranged by size: 

                 372     178      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     272     582 
   Size:        272     310
 
SgrAI Cr'CCGG_yG

Cuts at:      0     232     582 
   Size:        232     350
 
SmlI C'TyrA_G

Cuts at:      0     241     582 
   Size:        241     341
 
StuI AGG'CCT

Cuts at:      0      52     582 
   Size:         52     530
 
StyI C'CwwG_G

Cuts at:      0      47     582 
   Size:         47     535
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      19     169     582 
   Size:         19     150     413

  Fragments arranged by size: 

                 413     150      19
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     219     309     444     582 
   Size:        219      90     135     138

  Fragments arranged by size: 

                 219     138     135      90
 

 Enzymes that do cut:

     AgeI     ApoI     AvaI     BbsI     BglI    BmgBI     BmtI    BpuEI
    BsaWI    BseRI    BsrFI   BstEII    BstXI     EaeI     EciI EcoO109I
    EcoRI    HaeII     MslI     NaeI   NgoMIV     NheI     PpiI    PpuMI
    SgrAI     SmlI     StuI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI  EcoICRI  Eco57MI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NarI     NcoI     NdeI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI