Restriction map for
pF1KB9473
(Linear) (Six-base) MAPSORT of: KIBB9473.seq Check: 2761 from: 1 to: 2496
KIBB9473.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AccI GT'mk_AC
Cuts at: 0 152 2496
Size: 152 2344
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 818 1780 2496
Size: 517 301 962 716
Fragments arranged by size:
962 716 517 301
AleI CACnn'nnGTG
Cuts at: 0 2266 2496
Size: 2266 230
AlwNI CAG_nnn'CTG
Cuts at: 0 429 2315 2496
Size: 429 1886 181
Fragments arranged by size:
1886 429 181
ApoI r'AATT_y
Cuts at: 0 694 1325 1988 2496
Size: 694 631 663 508
Fragments arranged by size:
694 663 631 508
AvaI C'yCGr_G
Cuts at: 0 64 2339 2496
Size: 64 2275 157
Fragments arranged by size:
2275 157 64
BanI G'GyrC_C
Cuts at: 0 68 99 133 456 2496
Size: 68 31 34 323 2040
Fragments arranged by size:
2040 323 68 34 31
BanII G_rGCy'C
Cuts at: 0 1575 2496
Size: 1575 921
BbeI G_GCGC'C
Cuts at: 0 137 2496
Size: 137 2359
BbsI GAAGACnn'nnnn_
Cuts at: 0 933 2484 2496
Size: 933 1551 12
Fragments arranged by size:
1551 933 12
BbvCI CC'TCA_GC
Cuts at: 0 172 1927 2496
Size: 172 1755 569
Fragments arranged by size:
1755 569 172
BciVI GTATCCnnnnn_n'
Cuts at: 0 2038 2496
Size: 2038 458
Bme1580I G_kGCm'C
Cuts at: 0 71 667 2496
Size: 71 596 1829
Fragments arranged by size:
1829 596 71
BmgBI CAC'GTC
Cuts at: 0 588 2496
Size: 588 1908
BmrI ACTGGGnnnn_n'
Cuts at: 0 2230 2496
Size: 2230 266
BmtI G_CTAG'C
Cuts at: 0 1924 2496
Size: 1924 572
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1143 2496
Size: 1143 1353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1113 2496
Size: 1113 1383
Bpu10I CC'TnA_GC
Cuts at: 0 172 1927 2496
Size: 172 1755 569
Fragments arranged by size:
1755 569 172
BsaI GGTCTCn'nnnn_
Cuts at: 0 1633 2471 2496
Size: 1633 838 25
Fragments arranged by size:
1633 838 25
BsaAI yAC'GTr
Cuts at: 0 1512 2496
Size: 1512 984
BsaBI GATnn'nnATC
Cuts at: 0 143 2496
Size: 143 2353
BsaHI Gr'CG_yC
Cuts at: 0 58 134 1469 2496
Size: 58 76 1335 1027
Fragments arranged by size:
1335 1027 76 58
BsaWI w'CCGG_w
Cuts at: 0 512 1253 1298 2496
Size: 512 741 45 1198
Fragments arranged by size:
1198 741 512 45
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 366 1143 1216 2496
Size: 366 777 73 1280
Fragments arranged by size:
1280 777 366 73
BseYI C'CCAG_C
Cuts at: 0 2154 2315 2496
Size: 2154 161 181
Fragments arranged by size:
2154 181 161
BsiEI CG_ry'CG
Cuts at: 0 2333 2496
Size: 2333 163
BsiHKAI G_wGCw'C
Cuts at: 0 471 1458 1673 2496
Size: 471 987 215 823
Fragments arranged by size:
987 823 471 215
BsmI GAATG_Cn'
Cuts at: 0 2360 2496
Size: 2360 136
BsmBI CGTCTCn'nnnn_
Cuts at: 0 403 2496
Size: 403 2093
Bsp1286I G_dGCh'C
Cuts at: 0 71 471 667 1458 1575 1673 2496
Size: 71 400 196 791 117 98 823
Fragments arranged by size:
823 791 400 196 117 98 71
BspEI T'CCGG_A
Cuts at: 0 512 1253 2496
Size: 512 741 1243
Fragments arranged by size:
1243 741 512
BspHI T'CATG_A
Cuts at: 0 2204 2496
Size: 2204 292
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 309 2496
Size: 309 2187
BsrBI CCG'CTC
Cuts at: 0 2244 2496
Size: 2244 252
BsrDI GCAATG_nn'
Cuts at: 0 1073 1349 1646 2496
Size: 1073 276 297 850
Fragments arranged by size:
1073 850 297 276
BsrGI T'GTAC_A
Cuts at: 0 1733 2496
Size: 1733 763
BssHII G'CGCG_C
Cuts at: 0 32 2496
Size: 32 2464
BstEII G'GTnAC_C
Cuts at: 0 1839 2496
Size: 1839 657
BstXI CCAn_nnnn'nTGG
Cuts at: 0 588 2496
Size: 588 1908
BstYI r'GATC_y
Cuts at: 0 817 2483 2496
Size: 817 1666 13
Fragments arranged by size:
1666 817 13
BtgI C'CryG_G
Cuts at: 0 452 1196 2006 2496
Size: 452 744 810 490
Fragments arranged by size:
810 744 490 452
BtsI GCAGTG_nn'
Cuts at: 0 2041 2496
Size: 2041 455
DraIII CAC_nnn'GTG
Cuts at: 0 690 2496
Size: 690 1806
EaeI y'GGCC_r
Cuts at: 0 2258 2330 2496
Size: 2258 72 166
Fragments arranged by size:
2258 166 72
EagI C'GGCC_G
Cuts at: 0 2330 2496
Size: 2330 166
EarI CTCTTCn'nnn_
Cuts at: 0 337 1534 1636 2251 2496
Size: 337 1197 102 615 245
Fragments arranged by size:
1197 615 337 245 102
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 818 1113 1780 2496
Size: 517 301 295 667 716
Fragments arranged by size:
716 667 517 301 295
EcoO109I rG'GnC_Cy
Cuts at: 0 415 2229 2496
Size: 415 1814 267
Fragments arranged by size:
1814 415 267
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1608 2496
Size: 1608 888
HaeII r_GCGC'y
Cuts at: 0 137 1802 2496
Size: 137 1665 694
Fragments arranged by size:
1665 694 137
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 433 465 577 609 840 872
Size: 295 32 106 32 112 32 231 32
Cuts at: 872 2468 2496
Size: 1596 28
Fragments arranged by size:
1596 295 231 112 106 32 32 32
32 28
HincII GTy'rAC
Cuts at: 0 2094 2444 2496
Size: 2094 350 52
Fragments arranged by size:
2094 350 52
HindIII A'AGCT_T
Cuts at: 0 1026 2496
Size: 1026 1470
HpaI GTT'AAC
Cuts at: 0 2094 2444 2496
Size: 2094 350 52
Fragments arranged by size:
2094 350 52
KasI G'GCGC_C
Cuts at: 0 133 2496
Size: 133 2363
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1730 2496
Size: 1730 766
MscI TGG'CCA
Cuts at: 0 2260 2496
Size: 2260 236
MslI CAynn'nnrTG
Cuts at: 0 607 1354 1735 2203 2266 2496
Size: 607 747 381 468 63 230
Fragments arranged by size:
747 607 468 381 230 63
MspA1I CmG'CkG
Cuts at: 0 158 1383 2266 2315 2496
Size: 158 1225 883 49 181
Fragments arranged by size:
1225 883 181 158 49
NarI GG'CG_CC
Cuts at: 0 134 2496
Size: 134 2362
NcoI C'CATG_G
Cuts at: 0 452 2006 2496
Size: 452 1554 490
Fragments arranged by size:
1554 490 452
NheI G'CTAG_C
Cuts at: 0 1920 2496
Size: 1920 576
NspI r_CATG'y
Cuts at: 0 1758 2154 2496
Size: 1758 396 342
Fragments arranged by size:
1758 396 342
PasI CC'CwG_GG
Cuts at: 0 462 2132 2496
Size: 462 1670 364
Fragments arranged by size:
1670 462 364
PflMI CCAn_nnn'nTGG
Cuts at: 0 690 1911 2496
Size: 690 1221 585
Fragments arranged by size:
1221 690 585
PfoI T'CCnGG_A
Cuts at: 0 830 1134 1288 2496
Size: 830 304 154 1208
Fragments arranged by size:
1208 830 304 154
PmlI CAC'GTG
Cuts at: 0 1512 2496
Size: 1512 984
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 238 270 2496
Size: 175 32 31 32 2226
Fragments arranged by size:
2226 175 32 32 31
PpuMI rG'GwC_Cy
Cuts at: 0 415 2229 2496
Size: 415 1814 267
Fragments arranged by size:
1814 415 267
PshAI GACnn'nnGTC
Cuts at: 0 889 2496
Size: 889 1607
PvuII CAG'CTG
Cuts at: 0 1383 2315 2496
Size: 1383 932 181
Fragments arranged by size:
1383 932 181
SanDI GG'GwC_CC
Cuts at: 0 415 2496
Size: 415 2081
SapI GCTCTTCn'nnn_
Cuts at: 0 337 2251 2496
Size: 337 1914 245
Fragments arranged by size:
1914 337 245
SexAI A'CCwGG_T
Cuts at: 0 218 2496
Size: 218 2278
SfcI C'TryA_G
Cuts at: 0 153 742 859 2496
Size: 153 589 117 1637
Fragments arranged by size:
1637 589 153 117
SfoI GGC'GCC
Cuts at: 0 135 2496
Size: 135 2361
SmaI CCC'GGG
Cuts at: 0 66 2341 2496
Size: 66 2275 155
Fragments arranged by size:
2275 155 66
SphI G_CATG'C
Cuts at: 0 1758 2496
Size: 1758 738
SrfI GCCC'GGGC
Cuts at: 0 66 2496
Size: 66 2430
StyI C'CwwG_G
Cuts at: 0 336 452 1463 1583 1848 2006 2496
Size: 336 116 1011 120 265 158 490
Fragments arranged by size:
1011 490 336 265 158 120 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 454 1796 1948 2496
Size: 454 1342 152 548
Fragments arranged by size:
1342 548 454 152
TatI w'GTAC_w
Cuts at: 0 473 1733 2496
Size: 473 1260 763
Fragments arranged by size:
1260 763 473
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 372 404 1043 1075 2496
Size: 372 32 639 32 1421
Fragments arranged by size:
1421 639 372 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 459 2496
Size: 459 2037
XmaI C'CCGG_G
Cuts at: 0 64 2339 2496
Size: 64 2275 157
Fragments arranged by size:
2275 157 64
XmnI GAAnn'nnTTC
Cuts at: 0 502 2496
Size: 502 1994
Enzymes that do cut:
AccI AcuI AleI AlwNI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BciVI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BstEII BstXI
BstYI BtgI BtsI DraIII EaeI EagI EarI Eco57MI
EcoO109I FalI HaeII Hin4I HincII HindIII HpaI KasI
MmeI MscI MslI MspA1I NarI NcoI NheI NspI
PasI PflMI PfoI PmlI PpiI PpuMI PshAI PvuII
SanDI SapI SexAI SfcI SfoI SmaI SphI SrfI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BclI BfrBI BglI BglII
BlpI BpuEI BsaXI BsgI BsiWI BsrFI BssSI BstAPI
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
KpnI MfeI MluI NaeI NdeI NgoMIV NotI NruI
NsiI PacI PciI PmeI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacI SacII SalI SbfI ScaI
SfiI SgfI SgrAI SmlI SnaBI SpeI SspI StuI
SwaI Tth111I XbaI XhoI ZraI