Restriction map for
pF1KB9470
(Linear) (Six-base) MAPSORT of: KIBB9470.seq Check: 528 from: 1 to: 1965
KIBB9470.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 151 583 1965
Size: 136 15 432 1382
Fragments arranged by size:
1382 432 136 15
AfeI AGC'GCT
Cuts at: 0 169 1965
Size: 169 1796
AgeI A'CCGG_T
Cuts at: 0 924 1965
Size: 924 1041
AhdI GACnn_n'nnGTC
Cuts at: 0 1554 1965
Size: 1554 411
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1513 1965
Size: 1513 452
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 433 465 1249 1281 1965
Size: 433 32 784 32 684
Fragments arranged by size:
784 684 433 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 792 879 1143 1454 1674 1965
Size: 792 87 264 311 220 291
Fragments arranged by size:
792 311 291 264 220 87
ApaI G_GGCC'C
Cuts at: 0 36 109 941 1515 1959 1965
Size: 36 73 832 574 444 6
Fragments arranged by size:
832 574 444 73 36 6
ApaLI G'TGCA_C
Cuts at: 0 541 1965
Size: 541 1424
AvaI C'yCGr_G
Cuts at: 0 283 586 1317 1637 1965
Size: 283 303 731 320 328
Fragments arranged by size:
731 328 320 303 283
AvrII C'CTAG_G
Cuts at: 0 1920 1965
Size: 1920 45
BanI G'GyrC_C
Cuts at: 0 384 921 1221 1597 1948 1965
Size: 384 537 300 376 351 17
Fragments arranged by size:
537 384 376 351 300 17
BanII G_rGCy'C
Cuts at: 0 10 36 76 109 434 584 593 941
Size: 10 26 40 33 325 150 9 348
Cuts at: 941 985 1019 1324 1444 1515 1523 1616 1688
Size: 44 34 305 120 71 8 93 72
Cuts at: 1688 1959 1965
Size: 271 6
Fragments arranged by size:
348 325 305 271 150 120 93 72
71 44 40 34 33 26 10 9
8 6
BbeI G_GCGC'C
Cuts at: 0 388 1965
Size: 388 1577
BbvCI CC'TCA_GC
Cuts at: 0 625 1965
Size: 625 1340
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 849 883 1465 1499 1965
Size: 849 34 582 34 466
Fragments arranged by size:
849 582 466 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 31 1399 1965
Size: 31 1368 566
Fragments arranged by size:
1368 566 31
BlpI GC'TnA_GC
Cuts at: 0 4 1965
Size: 4 1961
Bme1580I G_kGCm'C
Cuts at: 0 36 94 109 545 667 941 1226 1358
Size: 36 58 15 436 122 274 285 132
Cuts at: 1358 1515 1959 1965
Size: 157 444 6
Fragments arranged by size:
444 436 285 274 157 132 122 58
36 15 6
BmgBI CAC'GTC
Cuts at: 0 540 1965
Size: 540 1425
BmrI ACTGGGnnnn_n'
Cuts at: 0 835 1142 1518 1965
Size: 835 307 376 447
Fragments arranged by size:
835 447 376 307
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1324 1965
Size: 1324 641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 1068 1237 1537 1965
Size: 691 377 169 300 428
Fragments arranged by size:
691 428 377 300 169
Bpu10I CC'TnA_GC
Cuts at: 0 625 1687 1965
Size: 625 1062 278
Fragments arranged by size:
1062 625 278
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1294 1965
Size: 1294 671
BsaI GGTCTCn'nnnn_
Cuts at: 0 1295 1965
Size: 1295 670
BsaHI Gr'CG_yC
Cuts at: 0 385 1965
Size: 385 1580
BsaWI w'CCGG_w
Cuts at: 0 924 1965
Size: 924 1041
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 433 463 1306 1336 1965
Size: 433 30 843 30 629
Fragments arranged by size:
843 629 433 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 156 454 1003 1224 1740 1887 1918 1965
Size: 156 298 549 221 516 147 31 47
Fragments arranged by size:
549 516 298 221 156 147 47 31
BseYI C'CCAG_C
Cuts at: 0 102 635 1384 1534 1615 1965
Size: 102 533 749 150 81 350
Fragments arranged by size:
749 533 350 150 102 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1137 1156 1348 1440 1483 1965
Size: 1137 19 192 92 43 482
Fragments arranged by size:
1137 482 192 92 43 19
BsiEI CG_ry'CG
Cuts at: 0 1070 1965
Size: 1070 895
BsiHKAI G_wGCw'C
Cuts at: 0 545 584 1082 1688 1965
Size: 545 39 498 606 277
Fragments arranged by size:
606 545 498 277 39
Bsp1286I G_dGCh'C
Cuts at: 0 10 36 76 94 109 434 545 584
Size: 10 26 40 18 15 325 111 39
Cuts at: 584 593 667 941 985 1019 1082 1226 1324
Size: 9 74 274 44 34 63 144 98
Cuts at: 1324 1358 1444 1515 1523 1616 1688 1959 1965
Size: 34 86 71 8 93 72 271 6
Fragments arranged by size:
325 274 271 144 111 98 93 86
74 72 71 63 44 40 39 34
34 26 18 15 10 9 8 6
BspHI T'CATG_A
Cuts at: 0 149 1965
Size: 149 1816
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 494 581 725 1965
Size: 494 87 144 1240
Fragments arranged by size:
1240 494 144 87
BsrBI CCG'CTC
Cuts at: 0 38 363 1530 1655 1965
Size: 38 325 1167 125 310
Fragments arranged by size:
1167 325 310 125 38
BsrDI GCAATG_nn'
Cuts at: 0 970 1965
Size: 970 995
BsrFI r'CCGG_y
Cuts at: 0 924 1112 1304 1965
Size: 924 188 192 661
Fragments arranged by size:
924 661 192 188
BssHII G'CGCG_C
Cuts at: 0 1648 1965
Size: 1648 317
BssSI C'ACGA_G
Cuts at: 0 358 1965
Size: 358 1607
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1143 1463 1948 1965
Size: 1143 320 485 17
Fragments arranged by size:
1143 485 320 17
BstEII G'GTnAC_C
Cuts at: 0 993 1965
Size: 993 972
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1560 1965
Size: 1560 405
BstYI r'GATC_y
Cuts at: 0 323 1965
Size: 323 1642
Bsu36I CC'TnA_GG
Cuts at: 0 701 1965
Size: 701 1264
BtgI C'CryG_G
Cuts at: 0 593 1070 1097 1965
Size: 593 477 27 868
Fragments arranged by size:
868 593 477 27
BtsI GCAGTG_nn'
Cuts at: 0 409 977 1965
Size: 409 568 988
Fragments arranged by size:
988 568 409
DraIII CAC_nnn'GTG
Cuts at: 0 549 1965
Size: 549 1416
EaeI y'GGCC_r
Cuts at: 0 823 1067 1391 1965
Size: 823 244 324 574
Fragments arranged by size:
823 574 324 244
EagI C'GGCC_G
Cuts at: 0 1067 1965
Size: 1067 898
EarI CTCTTCn'nnn_
Cuts at: 0 919 1056 1965
Size: 919 137 909
Fragments arranged by size:
919 909 137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 96 1965
Size: 96 1869
EcoICRI GAG'CTC
Cuts at: 0 582 1686 1965
Size: 582 1104 279
Fragments arranged by size:
1104 582 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 151 583 691 1068 1237 1537 1965
Size: 136 15 432 108 377 169 300 428
Fragments arranged by size:
432 428 377 300 169 136 108 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 606 700 1965
Size: 606 94 1265
Fragments arranged by size:
1265 606 94
EcoO109I rG'GnC_Cy
Cuts at: 0 32 106 288 483 837 937 957 1046
Size: 32 74 182 195 354 100 20 89
Cuts at: 1046 1178 1412 1512 1548 1955 1956 1965
Size: 132 234 100 36 407 1 9
Fragments arranged by size:
407 354 234 195 182 132 100 100
89 74 36 32 20 9 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 321 1179 1965
Size: 321 858 786
Fragments arranged by size:
858 786 321
FspI TGC'GCA
Cuts at: 0 969 1965
Size: 969 996
HaeII r_GCGC'y
Cuts at: 0 171 339 388 1965
Size: 171 168 49 1577
Fragments arranged by size:
1577 171 168 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1306 1338 1540 1572 1965
Size: 1306 32 202 32 393
Fragments arranged by size:
1306 393 202 32 32
HincII GTy'rAC
Cuts at: 0 771 1965
Size: 771 1194
HindIII A'AGCT_T
Cuts at: 0 1926 1965
Size: 1926 39
KasI G'GCGC_C
Cuts at: 0 384 1965
Size: 384 1581
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 1965
Size: 1132 833
MscI TGG'CCA
Cuts at: 0 1393 1965
Size: 1393 572
MspA1I CmG'CkG
Cuts at: 0 629 639 1454 1710 1884 1965
Size: 629 10 815 256 174 81
Fragments arranged by size:
815 629 256 174 81 10
NarI GG'CG_CC
Cuts at: 0 385 1965
Size: 385 1580
NspI r_CATG'y
Cuts at: 0 459 1965
Size: 459 1506
PasI CC'CwG_GG
Cuts at: 0 76 231 292 319 1123 1965
Size: 76 155 61 27 804 842
Fragments arranged by size:
842 804 155 76 61 27
PflMI CCAn_nnn'nTGG
Cuts at: 0 792 1578 1965
Size: 792 786 387
Fragments arranged by size:
792 786 387
PfoI T'CCnGG_A
Cuts at: 0 419 929 1965
Size: 419 510 1036
Fragments arranged by size:
1036 510 419
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 1965
Size: 433 32 1500
Fragments arranged by size:
1500 433 32
PpuMI rG'GwC_Cy
Cuts at: 0 288 483 837 1046 1178 1548 1965
Size: 288 195 354 209 132 370 417
Fragments arranged by size:
417 370 354 288 209 195 132
PspOMI G'GGCC_C
Cuts at: 0 32 105 937 1511 1955 1965
Size: 32 73 832 574 444 10
Fragments arranged by size:
832 574 444 73 32 10
PstI C_TGCA'G
Cuts at: 0 491 1965
Size: 491 1474
PvuII CAG'CTG
Cuts at: 0 629 639 1454 1884 1965
Size: 629 10 815 430 81
Fragments arranged by size:
815 629 430 81 10
SacI G_AGCT'C
Cuts at: 0 584 1688 1965
Size: 584 1104 277
Fragments arranged by size:
1104 584 277
SapI GCTCTTCn'nnn_
Cuts at: 0 919 1965
Size: 919 1046
SbfI CC_TGCA'GG
Cuts at: 0 491 1965
Size: 491 1474
SexAI A'CCwGG_T
Cuts at: 0 473 1965
Size: 473 1492
SfcI C'TryA_G
Cuts at: 0 487 1965
Size: 487 1478
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 31 1965
Size: 31 1934
SfoI GGC'GCC
Cuts at: 0 386 1965
Size: 386 1579
SmaI CCC'GGG
Cuts at: 0 1639 1965
Size: 1639 326
SmlI C'TyrA_G
Cuts at: 0 1309 1965
Size: 1309 656
SphI G_CATG'C
Cuts at: 0 459 1965
Size: 459 1506
StuI AGG'CCT
Cuts at: 0 1663 1965
Size: 1663 302
StyI C'CwwG_G
Cuts at: 0 647 1008 1920 1965
Size: 647 361 912 45
Fragments arranged by size:
912 647 361 45
XmaI C'CCGG_G
Cuts at: 0 1637 1965
Size: 1637 328
Enzymes that do cut:
AcuI AfeI AgeI AhdI AlfI AloI AlwNI ApaI
ApaLI AvaI AvrII BanI BanII BbeI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I HincII HindIII
KasI MmeI MscI MspA1I NarI NspI PasI PflMI
PfoI PpiI PpuMI PspOMI PstI PvuII SacI SapI
SbfI SexAI SfcI SfiI SfoI SmaI SmlI SphI
StuI StyI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AleI
ApoI AscI AseI AsiSI BaeI BamHI BbsI BciVI
BclI BfrBI BglII BmtI BsaAI BsaBI BsiWI BsmI
BsmBI BspEI BsrGI BstBI BstZ17I BtgZI ClaI CspCI
DraI DrdI EcoRI EcoRV FseI FspAI HpaI KpnI
MfeI MluI MslI NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
ScaI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI