Restriction map for
pF1KB9469
(Linear) (Six-base) MAPSORT of: KIBB9469.seq Check: 7704 from: 1 to: 2406
KIBB9469.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 973 2406
Size: 973 1433
AfeI AGC'GCT
Cuts at: 0 144 1713 1780 2406
Size: 144 1569 67 626
Fragments arranged by size:
1569 626 144 67
AflII C'TTAA_G
Cuts at: 0 745 2406
Size: 745 1661
AhdI GACnn_n'nnGTC
Cuts at: 0 1519 2406
Size: 1519 887
AlwNI CAG_nnn'CTG
Cuts at: 0 29 84 189 1257 2086 2406
Size: 29 55 105 1068 829 320
Fragments arranged by size:
1068 829 320 105 55 29
ApaI G_GGCC'C
Cuts at: 0 938 1589 2093 2406
Size: 938 651 504 313
Fragments arranged by size:
938 651 504 313
ApoI r'AATT_y
Cuts at: 0 100 175 265 351 397 1348 1639 2406
Size: 100 75 90 86 46 951 291 767
Fragments arranged by size:
951 767 291 100 90 86 75 46
AvaI C'yCGr_G
Cuts at: 0 725 1736 2406
Size: 725 1011 670
Fragments arranged by size:
1011 725 670
AvrII C'CTAG_G
Cuts at: 0 1443 2406
Size: 1443 963
BanII G_rGCy'C
Cuts at: 0 650 938 1589 1605 1991 2093 2406
Size: 650 288 651 16 386 102 313
Fragments arranged by size:
651 650 386 313 288 102 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 446 979 2406
Size: 446 533 1427
Fragments arranged by size:
1427 533 446
BglII A'GATC_T
Cuts at: 0 1685 2406
Size: 1685 721
BlpI GC'TnA_GC
Cuts at: 0 926 2406
Size: 926 1480
Bme1580I G_kGCm'C
Cuts at: 0 938 1589 2093 2406
Size: 938 651 504 313
Fragments arranged by size:
938 651 504 313
BmgBI CAC'GTC
Cuts at: 0 2007 2406
Size: 2007 399
BmrI ACTGGGnnnn_n'
Cuts at: 0 1326 2215 2406
Size: 1326 889 191
Fragments arranged by size:
1326 889 191
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1425 2144 2406
Size: 1425 719 262
Fragments arranged by size:
1425 719 262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 106 702 2174 2406
Size: 37 69 596 1472 232
Fragments arranged by size:
1472 596 232 69 37
Bpu10I CC'TnA_GC
Cuts at: 0 1604 2406
Size: 1604 802
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 2363 2406
Size: 49 2314 43
Fragments arranged by size:
2314 49 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 874 1186 1413 2115 2406
Size: 874 312 227 702 291
Fragments arranged by size:
874 702 312 291 227
BsaBI GATnn'nnATC
Cuts at: 0 1633 2406
Size: 1633 773
BsaHI Gr'CG_yC
Cuts at: 0 901 2406
Size: 901 1505
BsaWI w'CCGG_w
Cuts at: 0 356 2406
Size: 356 2050
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 665 695 2406
Size: 665 30 1711
Fragments arranged by size:
1711 665 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1938 2406
Size: 1938 468
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1700 2406
Size: 1700 706
BsiHKAI G_wGCw'C
Cuts at: 0 650 1538 1605 1676 2406
Size: 650 888 67 71 730
Fragments arranged by size:
888 730 650 71 67
BsmI GAATG_Cn'
Cuts at: 0 1269 2406
Size: 1269 1137
Bsp1286I G_dGCh'C
Cuts at: 0 650 938 1538 1589 1605 1676 1991 2093
Size: 650 288 600 51 16 71 315 102
Cuts at: 2093 2406
Size: 313
Fragments arranged by size:
650 600 315 313 288 102 71 51
16
BspEI T'CCGG_A
Cuts at: 0 356 2406
Size: 356 2050
BspHI T'CATG_A
Cuts at: 0 1379 2406
Size: 1379 1027
BsrBI CCG'CTC
Cuts at: 0 387 2406
Size: 387 2019
BsrDI GCAATG_nn'
Cuts at: 0 2304 2406
Size: 2304 102
BstYI r'GATC_y
Cuts at: 0 1685 2235 2406
Size: 1685 550 171
Fragments arranged by size:
1685 550 171
Bsu36I CC'TnA_GG
Cuts at: 0 1357 1703 2406
Size: 1357 346 703
Fragments arranged by size:
1357 703 346
BtgI C'CryG_G
Cuts at: 0 1085 2406
Size: 1085 1321
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 231 2406
Size: 231 2175
DrdI GACnn_nn'nnGTC
Cuts at: 0 192 2406
Size: 192 2214
EaeI y'GGCC_r
Cuts at: 0 1619 2406
Size: 1619 787
EarI CTCTTCn'nnn_
Cuts at: 0 725 2406
Size: 725 1681
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1721 2203 2406
Size: 1721 482 203
Fragments arranged by size:
1721 482 203
EcoICRI GAG'CTC
Cuts at: 0 648 1603 2406
Size: 648 955 803
Fragments arranged by size:
955 803 648
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 106 702 973 2174 2406
Size: 37 69 596 271 1201 232
Fragments arranged by size:
1201 596 271 232 69 37
EcoO109I rG'GnC_Cy
Cuts at: 0 934 935 1047 2228 2406
Size: 934 1 112 1181 178
Fragments arranged by size:
1181 934 178 112 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1567 2404 2406
Size: 1567 837 2
Fragments arranged by size:
1567 837 2
HaeII r_GCGC'y
Cuts at: 0 146 1715 1782 2406
Size: 146 1569 67 624
Fragments arranged by size:
1569 624 146 67
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1505 1537 1587 1619 2406
Size: 1505 32 50 32 787
Fragments arranged by size:
1505 787 50 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 1589 1866 1964 2406
Size: 708 881 277 98 442
Fragments arranged by size:
881 708 442 277 98
MslI CAynn'nnrTG
Cuts at: 0 1310 1594 1654 2406
Size: 1310 284 60 752
Fragments arranged by size:
1310 752 284 60
MspA1I CmG'CkG
Cuts at: 0 15 2406
Size: 15 2391
NcoI C'CATG_G
Cuts at: 0 1085 2406
Size: 1085 1321
NspI r_CATG'y
Cuts at: 0 779 2406
Size: 779 1627
PflMI CCAn_nnn'nTGG
Cuts at: 0 2086 2406
Size: 2086 320
PfoI T'CCnGG_A
Cuts at: 0 554 786 2190 2332 2359 2391 2406
Size: 554 232 1404 142 27 32 15
Fragments arranged by size:
1404 554 232 142 32 27 15
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1023 1055 2406
Size: 1023 32 1351
Fragments arranged by size:
1351 1023 32
PspOMI G'GGCC_C
Cuts at: 0 934 1585 2089 2406
Size: 934 651 504 317
Fragments arranged by size:
934 651 504 317
PstI C_TGCA'G
Cuts at: 0 194 1046 2033 2406
Size: 194 852 987 373
Fragments arranged by size:
987 852 373 194
SacI G_AGCT'C
Cuts at: 0 650 1605 2406
Size: 650 955 801
Fragments arranged by size:
955 801 650
ScaI AGT'ACT
Cuts at: 0 958 2406
Size: 958 1448
SexAI A'CCwGG_T
Cuts at: 0 2338 2406
Size: 2338 68
SfcI C'TryA_G
Cuts at: 0 190 493 1042 1386 2029 2406
Size: 190 303 549 344 643 377
Fragments arranged by size:
643 549 377 344 303 190
SmaI CCC'GGG
Cuts at: 0 727 2406
Size: 727 1679
SmlI C'TyrA_G
Cuts at: 0 64 745 2378 2406
Size: 64 681 1633 28
Fragments arranged by size:
1633 681 64 28
SspI AAT'ATT
Cuts at: 0 585 1185 1242 2406
Size: 585 600 57 1164
Fragments arranged by size:
1164 600 585 57
StuI AGG'CCT
Cuts at: 0 1965 2406
Size: 1965 441
StyI C'CwwG_G
Cuts at: 0 344 380 938 1085 1443 2406
Size: 344 36 558 147 358 963
Fragments arranged by size:
963 558 358 344 147 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 840 2406
Size: 840 1566
TatI w'GTAC_w
Cuts at: 0 956 2406
Size: 956 1450
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 644 676 2406
Size: 644 32 1730
Fragments arranged by size:
1730 644 32
Tth111I GACn'n_nGTC
Cuts at: 0 978 2406
Size: 978 1428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 903 2406
Size: 903 1503
XmaI C'CCGG_G
Cuts at: 0 725 2406
Size: 725 1681
XmnI GAAnn'nnTTC
Cuts at: 0 156 2406
Size: 156 2250
Enzymes that do cut:
AcuI AfeI AflII AhdI AlwNI ApaI ApoI AvaI
AvrII BanII BbsI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI
BsrBI BsrDI BstYI Bsu36I BtgI BtgZI DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I
MmeI MslI MspA1I NcoI NspI PflMI PfoI PpiI
PspOMI PstI SacI ScaI SexAI SfcI SmaI SmlI
SspI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AleI
AlfI AloI ApaLI AscI AseI AsiSI BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BmtI BsaAI BseYI BsiEI BsiWI BsmBI BspMI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtsI ClaI CspCI DraI DraIII EagI EcoNI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PpuMI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SwaI XbaI
XhoI ZraI