Restriction map for
pF1KB9468
(Linear) (Six-base) MAPSORT of: KIBB9468.seq Check: 4038 from: 1 to: 6432
KIBB9468.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AatII G_ACGT'C
Cuts at: 0 7 6432
Size: 7 6425
AccI GT'mk_AC
Cuts at: 0 5014 5264 6432
Size: 5014 250 1168
Fragments arranged by size:
5014 1168 250
AclI AA'CG_TT
Cuts at: 0 2107 4112 6432
Size: 2107 2005 2320
Fragments arranged by size:
2320 2107 2005
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1121 1183 1626 2113 2172 2885 3049 6432
Size: 1121 62 443 487 59 713 164 3383
Fragments arranged by size:
3383 1121 713 487 443 164 62 59
AfeI AGC'GCT
Cuts at: 0 4856 6432
Size: 4856 1576
AflII C'TTAA_G
Cuts at: 0 319 1726 6432
Size: 319 1407 4706
Fragments arranged by size:
4706 1407 319
AflIII A'CryG_T
Cuts at: 0 4164 6432
Size: 4164 2268
AleI CACnn'nnGTG
Cuts at: 0 333 3151 5086 6432
Size: 333 2818 1935 1346
Fragments arranged by size:
2818 1935 1346 333
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4543 4849 6432
Size: 4543 306 1583
Fragments arranged by size:
4543 1583 306
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2604 2636 6432
Size: 2604 32 3796
Fragments arranged by size:
3796 2604 32
AlwNI CAG_nnn'CTG
Cuts at: 0 21 1435 2908 4330 4572 6207 6393 6432
Size: 21 1414 1473 1422 242 1635 186 39
Fragments arranged by size:
1635 1473 1422 1414 242 186 39 21
ApaLI G'TGCA_C
Cuts at: 0 3265 6432
Size: 3265 3167
ApoI r'AATT_y
Cuts at: 0 837 1484 2016 2383 3205 4228 4333 4540
Size: 837 647 532 367 822 1023 105 207
Cuts at: 4540 5037 6106 6432
Size: 497 1069 326
Fragments arranged by size:
1069 1023 837 822 647 532 497 367
326 207 105
AseI AT'TA_AT
Cuts at: 0 458 1747 3760 6432
Size: 458 1289 2013 2672
Fragments arranged by size:
2672 2013 1289 458
AvaI C'yCGr_G
Cuts at: 0 618 5456 6432
Size: 618 4838 976
Fragments arranged by size:
4838 976 618
BamHI G'GATC_C
Cuts at: 0 2285 3231 6432
Size: 2285 946 3201
Fragments arranged by size:
3201 2285 946
BanI G'GyrC_C
Cuts at: 0 2515 3678 4181 6432
Size: 2515 1163 503 2251
Fragments arranged by size:
2515 2251 1163 503
BanII G_rGCy'C
Cuts at: 0 2324 3146 5579 5637 6432
Size: 2324 822 2433 58 795
Fragments arranged by size:
2433 2324 822 795 58
BbeI G_GCGC'C
Cuts at: 0 4185 6432
Size: 4185 2247
BbsI GAAGACnn'nnnn_
Cuts at: 0 2178 2465 2719 3766 4568 4909 6432
Size: 2178 287 254 1047 802 341 1523
Fragments arranged by size:
2178 1523 1047 802 341 287 254
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4291 4325 6137 6171 6432
Size: 4291 34 1812 34 261
Fragments arranged by size:
4291 1812 261 34 34
BclI T'GATC_A
Cuts at: 0 887 1776 4808 6432
Size: 887 889 3032 1624
Fragments arranged by size:
3032 1624 889 887
BfrBI ATG'CAT
Cuts at: 0 6143 6432
Size: 6143 289
BglII A'GATC_T
Cuts at: 0 552 1356 3573 6432
Size: 552 804 2217 2859
Fragments arranged by size:
2859 2217 804 552
BlpI GC'TnA_GC
Cuts at: 0 627 1453 6432
Size: 627 826 4979
Fragments arranged by size:
4979 826 627
Bme1580I G_kGCm'C
Cuts at: 0 2307 3269 3526 3928 6432
Size: 2307 962 257 402 2504
Fragments arranged by size:
2504 2307 962 402 257
BmgBI CAC'GTC
Cuts at: 0 4167 6432
Size: 4167 2265
BmrI ACTGGGnnnn_n'
Cuts at: 0 2284 4225 5065 6432
Size: 2284 1941 840 1367
Fragments arranged by size:
2284 1941 1367 840
BmtI G_CTAG'C
Cuts at: 0 220 1193 6432
Size: 220 973 5239
Fragments arranged by size:
5239 973 220
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3640 5181 5268 5598 6432
Size: 3640 1541 87 330 834
Fragments arranged by size:
3640 1541 834 330 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 527 2735 2809 3514 3785 4384 4672
Size: 361 166 2208 74 705 271 599 288
Cuts at: 4672 5087 5194 5238 5386 5635 5677 6403 6432
Size: 415 107 44 148 249 42 726 29
Fragments arranged by size:
2208 726 705 599 415 361 288 271
249 166 148 107 74 44 42 29
Bpu10I CC'TnA_GC
Cuts at: 0 9 6432
Size: 9 6423
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2657 6432
Size: 2657 3775
BsaI GGTCTCn'nnnn_
Cuts at: 0 4648 5256 6432
Size: 4648 608 1176
Fragments arranged by size:
4648 1176 608
BsaBI GATnn'nnATC
Cuts at: 0 750 4393 6432
Size: 750 3643 2039
Fragments arranged by size:
3643 2039 750
BsaHI Gr'CG_yC
Cuts at: 0 4 4182 6432
Size: 4 4178 2250
Fragments arranged by size:
4178 2250 4
BsaWI w'CCGG_w
Cuts at: 0 3945 6432
Size: 3945 2487
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 20 50 3983 4013 6432
Size: 20 30 3933 30 2419
Fragments arranged by size:
3933 2419 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2089 2092 2905 3853 4234 5131 6216 6432
Size: 2089 3 813 948 381 897 1085 216
Fragments arranged by size:
2089 1085 948 897 813 381 216 3
BseYI C'CCAG_C
Cuts at: 0 14 1916 5192 5200 5636 6020 6432
Size: 14 1902 3276 8 436 384 412
Fragments arranged by size:
3276 1902 436 412 384 14 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1882 2461 4241 4697 4975 5066 6432
Size: 1882 579 1780 456 278 91 1366
Fragments arranged by size:
1882 1780 1366 579 456 278 91
BsiHKAI G_wGCw'C
Cuts at: 0 647 920 2132 2324 3269 3432 4178 5579
Size: 647 273 1212 192 945 163 746 1401
Cuts at: 5579 6432
Size: 853
Fragments arranged by size:
1401 1212 945 853 746 647 273 192
163
BsmI GAATG_Cn'
Cuts at: 0 3299 5342 6432
Size: 3299 2043 1090
Fragments arranged by size:
3299 2043 1090
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1071 2139 5419 6432
Size: 1071 1068 3280 1013
Fragments arranged by size:
3280 1071 1068 1013
Bsp1286I G_dGCh'C
Cuts at: 0 647 920 2132 2307 2324 3146 3269 3432
Size: 647 273 1212 175 17 822 123 163
Cuts at: 3432 3526 3928 4178 5579 5637 6432
Size: 94 402 250 1401 58 795
Fragments arranged by size:
1401 1212 822 795 647 402 273 250
175 163 123 94 58 17
BspEI T'CCGG_A
Cuts at: 0 3945 6432
Size: 3945 2487
BspHI T'CATG_A
Cuts at: 0 1514 6432
Size: 1514 4918
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 5276 6432
Size: 5276 1156
BsrDI GCAATG_nn'
Cuts at: 0 392 2702 5825 6432
Size: 392 2310 3123 607
Fragments arranged by size:
3123 2310 607 392
BsrFI r'CCGG_y
Cuts at: 0 5863 6432
Size: 5863 569
BssHII G'CGCG_C
Cuts at: 0 4731 4733 5138 6432
Size: 4731 2 405 1294
Fragments arranged by size:
4731 1294 405 2
BssSI C'ACGA_G
Cuts at: 0 415 6432
Size: 415 6017
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1047 3552 6432
Size: 1047 2505 2880
Fragments arranged by size:
2880 2505 1047
BstEII G'GTnAC_C
Cuts at: 0 5160 6432
Size: 5160 1272
BstXI CCAn_nnnn'nTGG
Cuts at: 0 5247 6432
Size: 5247 1185
BstYI r'GATC_y
Cuts at: 0 552 1356 2025 2285 3131 3231 3573 5058
Size: 552 804 669 260 846 100 342 1485
Cuts at: 5058 6432
Size: 1374
Fragments arranged by size:
1485 1374 846 804 669 552 342 260
100
BstZ17I GTA'TAC
Cuts at: 0 5015 6432
Size: 5015 1417
Bsu36I CC'TnA_GG
Cuts at: 0 98 6233 6432
Size: 98 6135 199
Fragments arranged by size:
6135 199 98
BtgI C'CryG_G
Cuts at: 0 818 2345 3170 4691 5705 6164 6432
Size: 818 1527 825 1521 1014 459 268
Fragments arranged by size:
1527 1521 1014 825 818 459 268
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 699 6129 6432
Size: 699 5430 303
Fragments arranged by size:
5430 699 303
BtsI GCAGTG_nn'
Cuts at: 0 540 2243 2655 3450 4457 5081 6432
Size: 540 1703 412 795 1007 624 1351
Fragments arranged by size:
1703 1351 1007 795 624 540 412
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1775 1810 6432
Size: 1775 35 4622
Fragments arranged by size:
4622 1775 35
DraI TTT'AAA
Cuts at: 0 2153 3224 3453 4539 4628 6432
Size: 2153 1071 229 1086 89 1804
Fragments arranged by size:
2153 1804 1086 1071 229 89
DraIII CAC_nnn'GTG
Cuts at: 0 3427 4219 5874 6432
Size: 3427 792 1655 558
Fragments arranged by size:
3427 1655 792 558
EaeI y'GGCC_r
Cuts at: 0 107 1490 2342 4946 5183 5865 6161 6432
Size: 107 1383 852 2604 237 682 296 271
Fragments arranged by size:
2604 1383 852 682 296 271 237 107
EarI CTCTTCn'nnn_
Cuts at: 0 152 1040 1199 1751 2312 3232 4112 4634
Size: 152 888 159 552 561 920 880 522
Cuts at: 4634 5567 6095 6432
Size: 933 528 337
Fragments arranged by size:
933 920 888 880 561 552 528 522
337 159 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3865 6184 6432
Size: 3865 2319 248
Fragments arranged by size:
3865 2319 248
EcoICRI GAG'CTC
Cuts at: 0 2322 5577 6432
Size: 2322 3255 855
Fragments arranged by size:
3255 2322 855
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 527 1121 1183 1626 2113 2172 2735
Size: 361 166 594 62 443 487 59 563
Cuts at: 2735 2809 2885 3049 3514 3785 4384 4672 5087
Size: 74 76 164 465 271 599 288 415
Cuts at: 5087 5194 5238 5386 5635 5677 6403 6432
Size: 107 44 148 249 42 726 29
Fragments arranged by size:
726 599 594 563 487 465 443 415
361 288 271 249 166 164 148 107
76 74 62 59 44 42 29
EcoO109I rG'GnC_Cy
Cuts at: 0 2829 5619 5738 6432
Size: 2829 2790 119 694
Fragments arranged by size:
2829 2790 694 119
EcoRI G'AATT_C
Cuts at: 0 837 1484 6432
Size: 837 647 4948
Fragments arranged by size:
4948 837 647
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 181 1149 1162 1188 1945 5982 6432
Size: 181 968 13 26 757 4037 450
Fragments arranged by size:
4037 968 757 450 181 26 13
HaeII r_GCGC'y
Cuts at: 0 682 4185 4858 5182 6432
Size: 682 3503 673 324 1250
Fragments arranged by size:
3503 1250 682 673 324
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 20 52 685 717 780 812 1650 1682
Size: 20 32 633 32 63 32 838 32
Cuts at: 1682 1768 1800 1828 1860 2065 2097 2661 2693
Size: 86 32 28 32 205 32 564 32
Cuts at: 2693 2853 2885 3565 3597 3983 4015 4599 4631
Size: 160 32 680 32 386 32 584 32
Cuts at: 4631 6048 6080 6432
Size: 1417 32 352
Fragments arranged by size:
1417 838 680 633 584 564 386 352
205 160 86 63 32 32 32 32
32 32 32 32 32 32 32 32
32 28 20
HincII GTy'rAC
Cuts at: 0 3075 5801 6432
Size: 3075 2726 631
Fragments arranged by size:
3075 2726 631
HindIII A'AGCT_T
Cuts at: 0 2215 3376 4383 4748 5023 6432
Size: 2215 1161 1007 365 275 1409
Fragments arranged by size:
2215 1409 1161 1007 365 275
KasI G'GCGC_C
Cuts at: 0 4181 6432
Size: 4181 2251
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 3576 5646 6432
Size: 248 3328 2070 786
Fragments arranged by size:
3328 2070 786 248
MscI TGG'CCA
Cuts at: 0 1492 4948 5185 6163 6432
Size: 1492 3456 237 978 269
Fragments arranged by size:
3456 1492 978 269 237
MslI CAynn'nnrTG
Cuts at: 0 333 2560 3151 3905 4804 5086 6432
Size: 333 2227 591 754 899 282 1346
Fragments arranged by size:
2227 1346 899 754 591 333 282
MspA1I CmG'CkG
Cuts at: 0 21 1738 3151 6078 6432
Size: 21 1717 1413 2927 354
Fragments arranged by size:
2927 1717 1413 354 21
NaeI GCC'GGC
Cuts at: 0 5865 6432
Size: 5865 567
NarI GG'CG_CC
Cuts at: 0 4182 6432
Size: 4182 2250
NcoI C'CATG_G
Cuts at: 0 818 3170 4691 5705 6164 6432
Size: 818 2352 1521 1014 459 268
Fragments arranged by size:
2352 1521 1014 818 459 268
NgoMIV G'CCGG_C
Cuts at: 0 5863 6432
Size: 5863 569
NheI G'CTAG_C
Cuts at: 0 216 1189 6432
Size: 216 973 5243
Fragments arranged by size:
5243 973 216
NsiI A_TGCA'T
Cuts at: 0 6145 6432
Size: 6145 287
NspI r_CATG'y
Cuts at: 0 1050 6432
Size: 1050 5382
PacI TTA_AT'TAA
Cuts at: 0 1751 6432
Size: 1751 4681
PflMI CCAn_nnn'nTGG
Cuts at: 0 1185 3808 6432
Size: 1185 2623 2624
Fragments arranged by size:
2624 2623 1185
PfoI T'CCnGG_A
Cuts at: 0 3629 4169 4894 5612 6110 6432
Size: 3629 540 725 718 498 322
Fragments arranged by size:
3629 725 718 540 498 322
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3829 3861 6432
Size: 3829 32 2571
Fragments arranged by size:
3829 2571 32
PsiI TTA'TAA
Cuts at: 0 3256 6031 6432
Size: 3256 2775 401
Fragments arranged by size:
3256 2775 401
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 519 551 2783 2815 5980 6012 6432
Size: 519 32 2232 32 3165 32 420
Fragments arranged by size:
3165 2232 519 420 32 32 32
PstI C_TGCA'G
Cuts at: 0 693 926 2238 2650 2669 3644 4883 5294
Size: 693 233 1312 412 19 975 1239 411
Cuts at: 5294 6432
Size: 1138
Fragments arranged by size:
1312 1239 1138 975 693 412 411 233
19
PvuII CAG'CTG
Cuts at: 0 21 3151 6078 6432
Size: 21 3130 2927 354
Fragments arranged by size:
3130 2927 354 21
SacI G_AGCT'C
Cuts at: 0 2324 5579 6432
Size: 2324 3255 853
Fragments arranged by size:
3255 2324 853
SapI GCTCTTCn'nnn_
Cuts at: 0 2312 5567 6432
Size: 2312 3255 865
Fragments arranged by size:
3255 2312 865
ScaI AGT'ACT
Cuts at: 0 3062 4405 6432
Size: 3062 1343 2027
Fragments arranged by size:
3062 2027 1343
SexAI A'CCwGG_T
Cuts at: 0 5900 6432
Size: 5900 532
SfcI C'TryA_G
Cuts at: 0 689 922 2234 2646 2665 2950 3640 4094
Size: 689 233 1312 412 19 285 690 454
Cuts at: 4094 4489 4879 5290 6432
Size: 395 390 411 1142
Fragments arranged by size:
1312 1142 690 689 454 412 411 395
390 285 233 19
SfoI GGC'GCC
Cuts at: 0 4183 6432
Size: 4183 2249
SmaI CCC'GGG
Cuts at: 0 620 6432
Size: 620 5812
SmlI C'TyrA_G
Cuts at: 0 319 1726 2672 6432
Size: 319 1407 946 3760
Fragments arranged by size:
3760 1407 946 319
SpeI A'CTAG_T
Cuts at: 0 5377 6432
Size: 5377 1055
SspI AAT'ATT
Cuts at: 0 428 3941 6432
Size: 428 3513 2491
Fragments arranged by size:
3513 2491 428
StuI AGG'CCT
Cuts at: 0 4634 6432
Size: 4634 1798
StyI C'CwwG_G
Cuts at: 0 227 818 945 3170 3518 4691 5279 5705
Size: 227 591 127 2225 348 1173 588 426
Cuts at: 5705 6164 6432
Size: 459 268
Fragments arranged by size:
2225 1173 591 588 459 426 348 268
227 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2059 4207 6432
Size: 2059 2148 2225
Fragments arranged by size:
2225 2148 2059
TatI w'GTAC_w
Cuts at: 0 145 3060 4403 6432
Size: 145 2915 1343 2029
Fragments arranged by size:
2915 2029 1343 145
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5444 5476 6432
Size: 5444 32 956
Fragments arranged by size:
5444 956 32
XbaI T'CTAG_A
Cuts at: 0 64 2568 3243 3576 6432
Size: 64 2504 675 333 2856
Fragments arranged by size:
2856 2504 675 333 64
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2297 2833 6432
Size: 2297 536 3599
Fragments arranged by size:
3599 2297 536
XmaI C'CCGG_G
Cuts at: 0 618 6432
Size: 618 5814
XmnI GAAnn'nnTTC
Cuts at: 0 1271 2551 3365 3584 4439 5342 6317 6432
Size: 1271 1280 814 219 855 903 975 115
Fragments arranged by size:
1280 1271 975 903 855 814 219 115
ZraI GAC'GTC
Cuts at: 0 5 6432
Size: 5 6427
Enzymes that do cut:
AatII AccI AclI AcuI AfeI AflII AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AseI AvaI BamHI
BanI BanII BbeI BbsI BcgI BclI BfrBI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PacI PflMI PfoI PpiI PsiI PsrI PstI PvuII
SacI SapI ScaI SexAI SfcI SfoI SmaI SmlI
SpeI SspI StuI StyI TaqII TatI TstI XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AgeI AhdI ApaI AscI AsiSI AvrII
BaeI BbvCI BciVI BglI BsaAI BsiEI BsiWI BsrBI
BsrGI BstBI ClaI DrdI EagI EcoNI EcoRV FseI
FspI FspAI HpaI KpnI MfeI MluI NdeI NotI
NruI PasI PciI PmeI PmlI PpuMI PshAI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SbfI SfiI
SgfI SgrAI SnaBI SphI SrfI SwaI Tth111I XhoI