Restriction map for
pF1KB9456
(Linear) (Six-base) MAPSORT of: KIBB9456.seq Check: 7419 from: 1 to: 3450
KIBB9456.orf
With 182 enzymes: SgfI *
July 18, 2007 13:39 ..
AccI GT'mk_AC
Cuts at: 0 572 585 959 3450
Size: 572 13 374 2491
Fragments arranged by size:
2491 572 374 13
Acc65I G'GTAC_C
Cuts at: 0 556 823 3450
Size: 556 267 2627
Fragments arranged by size:
2627 556 267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 539 637 1367 1738 3450
Size: 17 522 98 730 371 1712
Fragments arranged by size:
1712 730 522 371 98 17
AflIII A'CryG_T
Cuts at: 0 1738 2307 3450
Size: 1738 569 1143
Fragments arranged by size:
1738 1143 569
AleI CACnn'nnGTG
Cuts at: 0 2371 2566 3450
Size: 2371 195 884
Fragments arranged by size:
2371 884 195
AlwNI CAG_nnn'CTG
Cuts at: 0 3210 3450
Size: 3210 240
ApaI G_GGCC'C
Cuts at: 0 784 2046 3450
Size: 784 1262 1404
Fragments arranged by size:
1404 1262 784
ApaLI G'TGCA_C
Cuts at: 0 1769 3133 3450
Size: 1769 1364 317
Fragments arranged by size:
1769 1364 317
ApoI r'AATT_y
Cuts at: 0 393 3450
Size: 393 3057
AvaI C'yCGr_G
Cuts at: 0 122 140 770 890 1233 3170 3450
Size: 122 18 630 120 343 1937 280
Fragments arranged by size:
1937 630 343 280 122 120 18
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2841 2874 3450
Size: 2841 33 576
Fragments arranged by size:
2841 576 33
BamHI G'GATC_C
Cuts at: 0 225 3450
Size: 225 3225
BanI G'GyrC_C
Cuts at: 0 556 738 823 856 1684 3450
Size: 556 182 85 33 828 1766
Fragments arranged by size:
1766 828 556 182 85 33
BanII G_rGCy'C
Cuts at: 0 754 784 2046 2380 3450
Size: 754 30 1262 334 1070
Fragments arranged by size:
1262 1070 754 334 30
BbsI GAAGACnn'nnnn_
Cuts at: 0 431 1085 2306 2626 2855 3450
Size: 431 654 1221 320 229 595
Fragments arranged by size:
1221 654 595 431 320 229
BbvCI CC'TCA_GC
Cuts at: 0 103 3450
Size: 103 3347
BciVI GTATCCnnnnn_n'
Cuts at: 0 2197 2869 3450
Size: 2197 672 581
Fragments arranged by size:
2197 672 581
BclI T'GATC_A
Cuts at: 0 3434 3450
Size: 3434 16
BfrBI ATG'CAT
Cuts at: 0 3 2934 3252 3450
Size: 3 2931 318 198
Fragments arranged by size:
2931 318 198 3
BglI GCCn_nnn'nGGC
Cuts at: 0 2546 3450
Size: 2546 904
BglII A'GATC_T
Cuts at: 0 1513 1521 3450
Size: 1513 8 1929
Fragments arranged by size:
1929 1513 8
BlpI GC'TnA_GC
Cuts at: 0 2429 3450
Size: 2429 1021
Bme1580I G_kGCm'C
Cuts at: 0 640 777 784 1773 2046 3137 3450
Size: 640 137 7 989 273 1091 313
Fragments arranged by size:
1091 989 640 313 273 137 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 227 787 3450
Size: 227 560 2663
Fragments arranged by size:
2663 560 227
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1100 1538 2034 2283 3450
Size: 665 435 438 496 249 1167
Fragments arranged by size:
1167 665 496 438 435 249
Bpu10I CC'TnA_GC
Cuts at: 0 103 3450
Size: 103 3347
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 907 3450
Size: 464 443 2543
Fragments arranged by size:
2543 464 443
BsaI GGTCTCn'nnnn_
Cuts at: 0 2010 2368 3450
Size: 2010 358 1082
Fragments arranged by size:
2010 1082 358
BsaHI Gr'CG_yC
Cuts at: 0 2592 3450
Size: 2592 858
BsaWI w'CCGG_w
Cuts at: 0 96 239 3225 3450
Size: 96 143 2986 225
Fragments arranged by size:
2986 225 143 96
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 1094 2317 3450
Size: 436 658 1223 1133
Fragments arranged by size:
1223 1133 658 436
BseYI C'CCAG_C
Cuts at: 0 745 1128 2698 3450
Size: 745 383 1570 752
Fragments arranged by size:
1570 752 745 383
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2228 2835 3450
Size: 2228 607 615
Fragments arranged by size:
2228 615 607
BsiHKAI G_wGCw'C
Cuts at: 0 1773 3137 3450
Size: 1773 1364 313
Fragments arranged by size:
1773 1364 313
BsiWI C'GTAC_G
Cuts at: 0 1008 3450
Size: 1008 2442
BsmI GAATG_Cn'
Cuts at: 0 1301 3450
Size: 1301 2149
Bsp1286I G_dGCh'C
Cuts at: 0 640 754 777 784 1773 2046 2380 3137
Size: 640 114 23 7 989 273 334 757
Cuts at: 3137 3450
Size: 313
Fragments arranged by size:
989 757 640 334 313 273 114 23
7
BspEI T'CCGG_A
Cuts at: 0 96 3225 3450
Size: 96 3129 225
Fragments arranged by size:
3129 225 96
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3218 3450
Size: 3218 232
BsrDI GCAATG_nn'
Cuts at: 0 719 1605 1883 1904 2709 3450
Size: 719 886 278 21 805 741
Fragments arranged by size:
886 805 741 719 278 21
BsrGI T'GTAC_A
Cuts at: 0 688 845 3450
Size: 688 157 2605
Fragments arranged by size:
2605 688 157
BstAPI GCAn_nnn'nTGC
Cuts at: 0 217 3450
Size: 217 3233
BstBI TT'CG_AA
Cuts at: 0 2001 3450
Size: 2001 1449
BstEII G'GTnAC_C
Cuts at: 0 1132 3450
Size: 1132 2318
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 207 1706 2536 2706 3450
Size: 27 180 1499 830 170 744
Fragments arranged by size:
1499 830 744 180 170 27
BstYI r'GATC_y
Cuts at: 0 225 1513 1521 1917 2825 3450
Size: 225 1288 8 396 908 625
Fragments arranged by size:
1288 908 625 396 225 8
Bsu36I CC'TnA_GG
Cuts at: 0 3344 3450
Size: 3344 106
BtgI C'CryG_G
Cuts at: 0 1705 2117 2190 3450
Size: 1705 412 73 1260
Fragments arranged by size:
1705 1260 412 73
BtsI GCAGTG_nn'
Cuts at: 0 2665 3266 3432 3450
Size: 2665 601 166 18
Fragments arranged by size:
2665 601 166 18
ClaI AT'CG_AT
Cuts at: 0 65 3450
Size: 65 3385
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1566 1601 3450
Size: 1566 35 1849
Fragments arranged by size:
1849 1566 35
DraIII CAC_nnn'GTG
Cuts at: 0 235 2802 3450
Size: 235 2567 648
Fragments arranged by size:
2567 648 235
DrdI GACnn_nn'nnGTC
Cuts at: 0 2308 3450
Size: 2308 1142
EaeI y'GGCC_r
Cuts at: 0 458 1067 1696 1830 2492 3450
Size: 458 609 629 134 662 958
Fragments arranged by size:
958 662 629 609 458 134
EarI CTCTTCn'nnn_
Cuts at: 0 494 3101 3450
Size: 494 2607 349
Fragments arranged by size:
2607 494 349
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2926 3261 3450
Size: 2926 335 189
Fragments arranged by size:
2926 335 189
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 539 637 665 1100 1367 1538 1738
Size: 17 522 98 28 435 267 171 200
Cuts at: 1738 2034 2283 3450
Size: 296 249 1167
Fragments arranged by size:
1167 522 435 296 267 249 200 171
98 28 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1357 2452 3450
Size: 1357 1095 998
Fragments arranged by size:
1357 1095 998
EcoO109I rG'GnC_Cy
Cuts at: 0 270 2043 2242 2874 2990 3207 3450
Size: 270 1773 199 632 116 217 243
Fragments arranged by size:
1773 632 270 243 217 199 116
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 884 916 1153 1185 1355 1387
Size: 91 32 761 32 237 32 170 32
Cuts at: 1387 2055 2087 3049 3081 3450
Size: 668 32 962 32 369
Fragments arranged by size:
962 761 668 369 237 170 91 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 698 1437 3285 3450
Size: 698 739 1848 165
Fragments arranged by size:
1848 739 698 165
HindIII A'AGCT_T
Cuts at: 0 2417 3450
Size: 2417 1033
HpaI GTT'AAC
Cuts at: 0 3285 3450
Size: 3285 165
KpnI G_GTAC'C
Cuts at: 0 560 827 3450
Size: 560 267 2623
Fragments arranged by size:
2623 560 267
MfeI C'AATT_G
Cuts at: 0 665 3046 3450
Size: 665 2381 404
Fragments arranged by size:
2381 665 404
MscI TGG'CCA
Cuts at: 0 460 1069 1698 1832 3450
Size: 460 609 629 134 1618
Fragments arranged by size:
1618 629 609 460 134
MslI CAynn'nnrTG
Cuts at: 0 25 911 1424 1704 2059 2371 2566 2734
Size: 25 886 513 280 355 312 195 168
Cuts at: 2734 2939 2974 3450
Size: 205 35 476
Fragments arranged by size:
886 513 476 355 312 280 205 195
168 35 25
MspA1I CmG'CkG
Cuts at: 0 643 745 2484 3450
Size: 643 102 1739 966
Fragments arranged by size:
1739 966 643 102
NcoI C'CATG_G
Cuts at: 0 1705 2117 3450
Size: 1705 412 1333
Fragments arranged by size:
1705 1333 412
NsiI A_TGCA'T
Cuts at: 0 5 2936 3254 3450
Size: 5 2931 318 196
Fragments arranged by size:
2931 318 196 5
NspI r_CATG'y
Cuts at: 0 954 1128 1622 1742 1982 2311 3012 3222
Size: 954 174 494 120 240 329 701 210
Cuts at: 3222 3450
Size: 228
Fragments arranged by size:
954 701 494 329 240 228 210 174
120
PciI A'CATG_T
Cuts at: 0 1738 2307 3450
Size: 1738 569 1143
Fragments arranged by size:
1738 1143 569
PflMI CCAn_nnn'nTGG
Cuts at: 0 790 2487 3450
Size: 790 1697 963
Fragments arranged by size:
1697 963 790
PfoI T'CCnGG_A
Cuts at: 0 1305 3203 3450
Size: 1305 1898 247
Fragments arranged by size:
1898 1305 247
PpuMI rG'GwC_Cy
Cuts at: 0 270 2242 2874 2990 3207 3450
Size: 270 1972 632 116 217 243
Fragments arranged by size:
1972 632 270 243 217 116
PshAI GACnn'nnGTC
Cuts at: 0 92 275 3450
Size: 92 183 3175
Fragments arranged by size:
3175 183 92
PspOMI G'GGCC_C
Cuts at: 0 780 2042 3450
Size: 780 1262 1408
Fragments arranged by size:
1408 1262 780
PspXI vC'TCGA_Gb
Cuts at: 0 1233 3450
Size: 1233 2217
PvuII CAG'CTG
Cuts at: 0 643 745 2484 3450
Size: 643 102 1739 966
Fragments arranged by size:
1739 966 643 102
SmaI CCC'GGG
Cuts at: 0 142 772 3172 3450
Size: 142 630 2400 278
Fragments arranged by size:
2400 630 278 142
SmlI C'TyrA_G
Cuts at: 0 479 922 1233 3450
Size: 479 443 311 2217
Fragments arranged by size:
2217 479 443 311
SphI G_CATG'C
Cuts at: 0 1128 3450
Size: 1128 2322
SspI AAT'ATT
Cuts at: 0 382 594 3450
Size: 382 212 2856
Fragments arranged by size:
2856 382 212
StuI AGG'CCT
Cuts at: 0 2268 3450
Size: 2268 1182
StyI C'CwwG_G
Cuts at: 0 852 1178 1358 1705 1796 2117 2157 2541
Size: 852 326 180 347 91 321 40 384
Cuts at: 2541 3450
Size: 909
Fragments arranged by size:
909 852 384 347 326 321 180 91
40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 246 444 556 618 1698 2022 3074 3450
Size: 246 198 112 62 1080 324 1052 376
Fragments arranged by size:
1080 1052 376 324 246 198 112 62
TatI w'GTAC_w
Cuts at: 0 688 845 2144 3158 3450
Size: 688 157 1299 1014 292
Fragments arranged by size:
1299 1014 688 292 157
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 24 204 732 1211 1303 2115 3450
Size: 24 180 528 479 92 812 1335
Fragments arranged by size:
1335 812 528 479 180 92 24
XhoI C'TCGA_G
Cuts at: 0 1233 3450
Size: 1233 2217
XmaI C'CCGG_G
Cuts at: 0 140 770 3170 3450
Size: 140 630 2400 280
Fragments arranged by size:
2400 630 280 140
XmnI GAAnn'nnTTC
Cuts at: 0 869 1427 3450
Size: 869 558 2023
Fragments arranged by size:
2023 869 558
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspMI BsrDI
BsrGI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtsI ClaI CspCI DraIII DrdI EaeI EarI EciI
Eco57MI EcoNI EcoO109I Hin4I HincII HindIII HpaI KpnI
MfeI MscI MslI MspA1I NcoI NsiI NspI PciI
PflMI PfoI PpuMI PshAI PspOMI PspXI PvuII SmaI
SmlI SphI SspI StuI StyI TaqII TatI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BbeI BcgI BmgBI
BmtI BplI BsaAI BsaBI BsaXI BsiEI BsmBI BspHI
BsrBI BsrFI BssHII BssSI BstZ17I BtgZI DraI EagI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
KasI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PasI PmeI PmlI PpiI PsiI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SwaI TstI Tth111I XbaI
ZraI