Restriction map for
pF1KB9454
(Linear) (Six-base) MAPSORT of: KIBB9454.seq Check: 4749 from: 1 to: 1557
KIBB9454.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AccI GT'mk_AC
Cuts at: 0 50 1557
Size: 50 1507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 797 1557
Size: 91 706 760
Fragments arranged by size:
760 706 91
AflIII A'CryG_T
Cuts at: 0 1069 1557
Size: 1069 488
AleI CACnn'nnGTG
Cuts at: 0 1024 1557
Size: 1024 533
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1324 1401 1557
Size: 1324 77 156
Fragments arranged by size:
1324 156 77
ApoI r'AATT_y
Cuts at: 0 450 1557
Size: 450 1107
BanII G_rGCy'C
Cuts at: 0 911 1362 1557
Size: 911 451 195
Fragments arranged by size:
911 451 195
BbsI GAAGACnn'nnnn_
Cuts at: 0 110 1066 1557
Size: 110 956 491
Fragments arranged by size:
956 491 110
BbvCI CC'TCA_GC
Cuts at: 0 785 1557
Size: 785 772
BglII A'GATC_T
Cuts at: 0 1305 1470 1557
Size: 1305 165 87
Fragments arranged by size:
1305 165 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 721 955 1557
Size: 721 234 602
Fragments arranged by size:
721 602 234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 776 1557
Size: 614 162 781
Fragments arranged by size:
781 614 162
Bpu10I CC'TnA_GC
Cuts at: 0 652 785 1557
Size: 652 133 772
Fragments arranged by size:
772 652 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1088 1125 1557
Size: 1088 37 432
Fragments arranged by size:
1088 432 37
BsaI GGTCTCn'nnnn_
Cuts at: 0 821 1557
Size: 821 736
BsaAI yAC'GTr
Cuts at: 0 1230 1557
Size: 1230 327
BsaWI w'CCGG_w
Cuts at: 0 6 1557
Size: 6 1551
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 587 617 749 779 818 848 1557
Size: 587 30 132 30 39 30 709
Fragments arranged by size:
709 587 132 39 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 336 354 712 1557
Size: 336 18 358 845
Fragments arranged by size:
845 358 336 18
BseYI C'CCAG_C
Cuts at: 0 1494 1557
Size: 1494 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 686 1557
Size: 686 871
BsiHKAI G_wGCw'C
Cuts at: 0 770 1362 1557
Size: 770 592 195
Fragments arranged by size:
770 592 195
BsmI GAATG_Cn'
Cuts at: 0 1003 1557
Size: 1003 554
Bsp1286I G_dGCh'C
Cuts at: 0 770 911 1362 1557
Size: 770 141 451 195
Fragments arranged by size:
770 451 195 141
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 658 1557
Size: 658 899
BsrGI T'GTAC_A
Cuts at: 0 507 1557
Size: 507 1050
BstYI r'GATC_y
Cuts at: 0 1305 1470 1557
Size: 1305 165 87
Fragments arranged by size:
1305 165 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 882 1557
Size: 882 675
BtsI GCAGTG_nn'
Cuts at: 0 1509 1557
Size: 1509 48
DraI TTT'AAA
Cuts at: 0 90 1557
Size: 90 1467
DrdI GACnn_nn'nnGTC
Cuts at: 0 1462 1557
Size: 1462 95
EarI CTCTTCn'nnn_
Cuts at: 0 336 354 363 372 381 387 405 583
Size: 336 18 9 9 9 6 18 178
Cuts at: 583 1557
Size: 974
Fragments arranged by size:
974 336 178 18 18 9 9 9
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 213 1557
Size: 213 1344
EcoICRI GAG'CTC
Cuts at: 0 1360 1557
Size: 1360 197
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 614 776 797 1557
Size: 91 523 162 21 760
Fragments arranged by size:
760 523 162 91 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 116 148 313 345 818 850 1557
Size: 116 32 165 32 473 32 707
Fragments arranged by size:
707 473 165 116 32 32 32
MslI CAynn'nnrTG
Cuts at: 0 98 1024 1557
Size: 98 926 533
Fragments arranged by size:
926 533 98
NspI r_CATG'y
Cuts at: 0 1073 1557
Size: 1073 484
PasI CC'CwG_GG
Cuts at: 0 1530 1557
Size: 1530 27
PciI A'CATG_T
Cuts at: 0 1069 1557
Size: 1069 488
PfoI T'CCnGG_A
Cuts at: 0 591 1557
Size: 591 966
PshAI GACnn'nnGTC
Cuts at: 0 46 1330 1557
Size: 46 1284 227
Fragments arranged by size:
1284 227 46
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 410 442 1557
Size: 410 32 1115
Fragments arranged by size:
1115 410 32
SacI G_AGCT'C
Cuts at: 0 1362 1557
Size: 1362 195
SfcI C'TryA_G
Cuts at: 0 844 1038 1557
Size: 844 194 519
Fragments arranged by size:
844 519 194
SmlI C'TyrA_G
Cuts at: 0 1103 1140 1557
Size: 1103 37 417
Fragments arranged by size:
1103 417 37
SnaBI TAC'GTA
Cuts at: 0 1230 1557
Size: 1230 327
StuI AGG'CCT
Cuts at: 0 815 1557
Size: 815 742
StyI C'CwwG_G
Cuts at: 0 279 1557
Size: 279 1278
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 22 73 853 1220 1486 1557
Size: 22 51 780 367 266 71
Fragments arranged by size:
780 367 266 71 51 22
TatI w'GTAC_w
Cuts at: 0 507 1506 1557
Size: 507 999 51
Fragments arranged by size:
999 507 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 648 680 749 781 1557
Size: 648 32 69 32 776
Fragments arranged by size:
776 648 69 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 832 1557
Size: 832 725
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1527 1557
Size: 1527 30
XmnI GAAnn'nnTTC
Cuts at: 0 548 1557
Size: 548 1009
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI ApoI BanII BbsI
BbvCI BglII BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrGI BstYI BtgZI BtsI DraI DrdI EarI
EciI EcoICRI Eco57MI Hin4I MslI NspI PasI PciI
PfoI PshAI PsrI SacI SfcI SmlI SnaBI StuI
StyI TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BcgI BciVI BclI
BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI BsaBI
BsaHI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EaeI EagI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflMI PmeI PmlI PpiI
PpuMI PsiI PspOMI PspXI PstI PvuI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SpeI SphI SrfI SspI
SwaI XbaI XhoI XmaI ZraI