Restriction map for
pF1KB9446
(Linear) (Six-base) MAPSORT of: KIBB9446.seq Check: 1467 from: 1 to: 969
KIBB9446.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
Acc65I G'GTAC_C
Cuts at: 0 688 969
Size: 688 281
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 969
Size: 503 466
AhdI GACnn_n'nnGTC
Cuts at: 0 296 969
Size: 296 673
ApaI G_GGCC'C
Cuts at: 0 414 969
Size: 414 555
ApoI r'AATT_y
Cuts at: 0 28 706 731 900 969
Size: 28 678 25 169 69
Fragments arranged by size:
678 169 69 28 25
AvaI C'yCGr_G
Cuts at: 0 413 969
Size: 413 556
BanI G'GyrC_C
Cuts at: 0 3 688 969
Size: 3 685 281
Fragments arranged by size:
685 281 3
BanII G_rGCy'C
Cuts at: 0 414 969
Size: 414 555
BbeI G_GCGC'C
Cuts at: 0 7 969
Size: 7 962
BbsI GAAGACnn'nnnn_
Cuts at: 0 295 969
Size: 295 674
BbvCI CC'TCA_GC
Cuts at: 0 389 969
Size: 389 580
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 35 69 969
Size: 35 34 900
Fragments arranged by size:
900 35 34
Bme1580I G_kGCm'C
Cuts at: 0 414 969
Size: 414 555
BmrI ACTGGGnnnn_n'
Cuts at: 0 522 969
Size: 522 447
Bpu10I CC'TnA_GC
Cuts at: 0 389 969
Size: 389 580
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 969
Size: 601 368
BsaI GGTCTCn'nnnn_
Cuts at: 0 376 708 969
Size: 376 332 261
Fragments arranged by size:
376 332 261
BsaAI yAC'GTr
Cuts at: 0 195 969
Size: 195 774
BsaHI Gr'CG_yC
Cuts at: 0 4 969
Size: 4 965
BsaWI w'CCGG_w
Cuts at: 0 171 969
Size: 171 798
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 386 932 969
Size: 386 546 37
Fragments arranged by size:
546 386 37
BseYI C'CCAG_C
Cuts at: 0 692 969
Size: 692 277
Bsp1286I G_dGCh'C
Cuts at: 0 414 969
Size: 414 555
BspEI T'CCGG_A
Cuts at: 0 171 969
Size: 171 798
BspHI T'CATG_A
Cuts at: 0 449 969
Size: 449 520
BsrDI GCAATG_nn'
Cuts at: 0 821 969
Size: 821 148
BssSI C'ACGA_G
Cuts at: 0 608 969
Size: 608 361
BstXI CCAn_nnnn'nTGG
Cuts at: 0 772 969
Size: 772 197
BstYI r'GATC_y
Cuts at: 0 420 969
Size: 420 549
BtgI C'CryG_G
Cuts at: 0 496 969
Size: 496 473
EarI CTCTTCn'nnn_
Cuts at: 0 74 479 838 969
Size: 74 405 359 131
Fragments arranged by size:
405 359 131 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 969
Size: 503 466
EcoO109I rG'GnC_Cy
Cuts at: 0 15 410 969
Size: 15 395 559
Fragments arranged by size:
559 395 15
EcoRI G'AATT_C
Cuts at: 0 900 969
Size: 900 69
HaeII r_GCGC'y
Cuts at: 0 7 781 969
Size: 7 774 188
Fragments arranged by size:
774 188 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 282 314 969
Size: 282 32 655
Fragments arranged by size:
655 282 32
KasI G'GCGC_C
Cuts at: 0 3 969
Size: 3 966
KpnI G_GTAC'C
Cuts at: 0 692 969
Size: 692 277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 124 969
Size: 124 845
MslI CAynn'nnrTG
Cuts at: 0 137 749 969
Size: 137 612 220
Fragments arranged by size:
612 220 137
NarI GG'CG_CC
Cuts at: 0 4 969
Size: 4 965
PfoI T'CCnGG_A
Cuts at: 0 32 969
Size: 32 937
PpuMI rG'GwC_Cy
Cuts at: 0 15 969
Size: 15 954
PspOMI G'GGCC_C
Cuts at: 0 410 969
Size: 410 559
SapI GCTCTTCn'nnn_
Cuts at: 0 479 838 969
Size: 479 359 131
Fragments arranged by size:
479 359 131
ScaI AGT'ACT
Cuts at: 0 185 969
Size: 185 784
SfoI GGC'GCC
Cuts at: 0 5 969
Size: 5 964
SmaI CCC'GGG
Cuts at: 0 415 969
Size: 415 554
SmlI C'TyrA_G
Cuts at: 0 580 969
Size: 580 389
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 771 969
Size: 771 198
TatI w'GTAC_w
Cuts at: 0 183 969
Size: 183 786
XmaI C'CCGG_G
Cuts at: 0 413 969
Size: 413 556
XmnI GAAnn'nnTTC
Cuts at: 0 124 167 969
Size: 124 43 802
Fragments arranged by size:
802 124 43
Enzymes that do cut:
Acc65I AcuI AhdI ApaI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BcgI Bme1580I BmrI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BseRI BseYI Bsp1286I BspEI
BspHI BsrDI BssSI BstXI BstYI BtgI EarI Eco57MI
EcoO109I EcoRI HaeII Hin4I KasI KpnI MmeI MslI
NarI PfoI PpuMI PspOMI SapI ScaI SfoI SmaI
SmlI TaqII TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BplI BpmI BsaBI BsaXI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspMI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI MfeI MluI MscI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI SexAI SfcI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TstI Tth111I XbaI XcmI XhoI ZraI