(Linear) (Six-base) MAPSORT of: KIBB9445.seq  Check: 490  from: 1  to: 2016



KIBB9445.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     794    2016 
   Size:        794    1222
 
AlwNI CAG_nnn'CTG

Cuts at:      0     667    1264    2016 
   Size:        667     597     752

  Fragments arranged by size: 

                 752     667     597
 
ApoI r'AATT_y

Cuts at:      0     124     239     331    1169    1622    1993    2016 
   Size:        124     115      92     838     453     371      23

  Fragments arranged by size: 

                 838     453     371     124     115      92      23
 
AseI AT'TA_AT

Cuts at:      0    1111    2016 
   Size:       1111     905
 
BanII G_rGCy'C

Cuts at:      0     135    2016 
   Size:        135    1881
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     122    1688    2016 
   Size:        122    1566     328

  Fragments arranged by size: 

                1566     328     122
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1574    2016 
   Size:       1574     442
 
BclI T'GATC_A

Cuts at:      0    1443    2016 
   Size:       1443     573
 
BfrBI ATG'CAT

Cuts at:      0    1792    2016 
   Size:       1792     224
 
BglII A'GATC_T

Cuts at:      0     227    2016 
   Size:        227    1789
 
BmtI G_CTAG'C

Cuts at:      0     701    2016 
   Size:        701    1315
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1014    1859    2016 
   Size:       1014     845     157

  Fragments arranged by size: 

                1014     845     157
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1396    2016 
   Size:       1396     620
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1546    1576    1630    1660    2016 
   Size:       1546      30      54      30     356

  Fragments arranged by size: 

                1546     356      54      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1000    1003    1006    2016 
   Size:       1000       3       3    1010

  Fragments arranged by size: 

                1010    1000       3       3
 
BseYI C'CCAG_C

Cuts at:      0     190    2016 
   Size:        190    1826
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     949    2016 
   Size:        949    1067
 
BsiEI CG_ry'CG

Cuts at:      0    1046    2016 
   Size:       1046     970
 
Bsp1286I G_dGCh'C

Cuts at:      0     135    2016 
   Size:        135    1881
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1757    2016 
   Size:       1757     259
 
BsrDI GCAATG_nn'

Cuts at:      0    1832    2016 
   Size:       1832     184
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     694    2016 
   Size:        694    1322
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     475    1916    2016 
   Size:        475    1441     100

  Fragments arranged by size: 

                1441     475     100
 
BstYI r'GATC_y

Cuts at:      0     227    1725    2016 
   Size:        227    1498     291

  Fragments arranged by size: 

                1498     291     227
 
BtgI C'CryG_G

Cuts at:      0      81    2016 
   Size:         81    1935
 
DraI TTT'AAA

Cuts at:      0    1959    2016 
   Size:       1959      57
 
DraIII CAC_nnn'GTG

Cuts at:      0     694    2016 
   Size:        694    1322
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1045    2016 
   Size:       1045     971
 
EaeI y'GGCC_r

Cuts at:      0      78    1043    2016 
   Size:         78     965     973

  Fragments arranged by size: 

                 973     965      78
 
EagI C'GGCC_G

Cuts at:      0    1043    2016 
   Size:       1043     973
 
EarI CTCTTCn'nnn_

Cuts at:      0      94     184     915     977    2016 
   Size:         94      90     731      62    1039

  Fragments arranged by size: 

                1039     731      94      90      62
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     794    1014    1859    2016 
   Size:        794     220     845     157

  Fragments arranged by size: 

                 845     794     220     157
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1358    1877    2016 
   Size:       1358     519     139

  Fragments arranged by size: 

                1358     519     139
 
EcoO109I rG'GnC_Cy

Cuts at:      0     664    2016 
   Size:        664    1352
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1168    2016 
   Size:       1168     848
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     212     244    2016 
   Size:        212      32    1772

  Fragments arranged by size: 

                1772     212      32
 
HincII GTy'rAC

Cuts at:      0    1857    2016 
   Size:       1857     159
 
HindIII A'AGCT_T

Cuts at:      0    1521    1744    2016 
   Size:       1521     223     272

  Fragments arranged by size: 

                1521     272     223
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     841    2016 
   Size:        841    1175
 
MscI TGG'CCA

Cuts at:      0      80    2016 
   Size:         80    1936
 
NcoI C'CATG_G

Cuts at:      0      81    2016 
   Size:         81    1935
 
NdeI CA'TA_TG

Cuts at:      0     902    1506    2016 
   Size:        902     604     510

  Fragments arranged by size: 

                 902     604     510
 
NheI G'CTAG_C

Cuts at:      0     697    2016 
   Size:        697    1319
 
NsiI A_TGCA'T

Cuts at:      0    1794    2016 
   Size:       1794     222
 
PacI TTA_AT'TAA

Cuts at:      0     305    2016 
   Size:        305    1711
 
PsiI TTA'TAA

Cuts at:      0     217     499    1999    2016 
   Size:        217     282    1500      17

  Fragments arranged by size: 

                1500     282     217      17
 
SapI GCTCTTCn'nnn_

Cuts at:      0     184    2016 
   Size:        184    1832
 
ScaI AGT'ACT

Cuts at:      0      40    1983    2016 
   Size:         40    1943      33

  Fragments arranged by size: 

                1943      40      33
 
SfcI C'TryA_G

Cuts at:      0      26     255    2016 
   Size:         26     229    1761

  Fragments arranged by size: 

                1761     229      26
 
SmlI C'TyrA_G

Cuts at:      0    1375    2016 
   Size:       1375     641
 
SspI AAT'ATT

Cuts at:      0     144     837    2016 
   Size:        144     693    1179

  Fragments arranged by size: 

                1179     693     144
 
StyI C'CwwG_G

Cuts at:      0      81    2016 
   Size:         81    1935
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     134    2016 
   Size:        134    1882
 
TatI w'GTAC_w

Cuts at:      0      38    1981    2016 
   Size:         38    1943      35

  Fragments arranged by size: 

                1943      38      35
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AseI    BanII     BbsI    BciVI     BclI
    BfrBI    BglII     BmtI     BpmI    BpuEI    BsaXI    BseRI    BseYI
     BsgI    BsiEI Bsp1286I    BspMI    BsrDI   BstAPI    BstXI    BstYI
     BtgI     DraI   DraIII     DrdI     EaeI     EagI     EarI  Eco57MI
    EcoNI EcoO109I     FalI    Hin4I   HincII  HindIII     MmeI     MscI
     NcoI     NdeI     NheI     NsiI     PacI     PsiI     SapI     ScaI
     SfcI     SmlI     SspI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI     BglI     BlpI Bme1580I    BmgBI     BmrI     BplI   Bpu10I
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
     HpaI     KasI     KpnI     MfeI     MluI     MslI   MspA1I     NaeI
     NarI   NgoMIV     NotI     NruI     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI