(Linear) (Six-base) MAPSORT of: KIBB9442.seq  Check: 9479  from: 1  to: 474



KIBB9442.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     100     474 
   Size:         10      90     374

  Fragments arranged by size: 

                 374      90      10
 
ApoI r'AATT_y

Cuts at:      0     441     474 
   Size:        441      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     404     474 
   Size:        404      70
 
BglII A'GATC_T

Cuts at:      0     429     474 
   Size:        429      45
 
Bpu10I CC'TnA_GC

Cuts at:      0      32     474 
   Size:         32     442
 
BsaWI w'CCGG_w

Cuts at:      0      38     474 
   Size:         38     436
 
BspEI T'CCGG_A

Cuts at:      0      38     474 
   Size:         38     436
 
BstYI r'GATC_y

Cuts at:      0     429     474 
   Size:        429      45
 
Bsu36I CC'TnA_GG

Cuts at:      0     337     474 
   Size:        337     137
 
BtgI C'CryG_G

Cuts at:      0     361     474 
   Size:        361     113
 
EarI CTCTTCn'nnn_

Cuts at:      0     158     474 
   Size:        158     316
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      10     100     474 
   Size:         10      90     374

  Fragments arranged by size: 

                 374      90      10
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     247     474 
   Size:        247     227
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     439     471     474 
   Size:        439      32       3

  Fragments arranged by size: 

                 439      32       3
 
HindIII A'AGCT_T

Cuts at:      0     112     224     474 
   Size:        112     112     250

  Fragments arranged by size: 

                 250     112     112
 
MslI CAynn'nnrTG

Cuts at:      0     286     474 
   Size:        286     188
 
MspA1I CmG'CkG

Cuts at:      0     100     474 
   Size:        100     374
 
PfoI T'CCnGG_A

Cuts at:      0     294     474 
   Size:        294     180
 
PvuII CAG'CTG

Cuts at:      0     100     474 
   Size:        100     374
 
SspI AAT'ATT

Cuts at:      0     302     474 
   Size:        302     172
 
XmnI GAAnn'nnTTC

Cuts at:      0     290     474 
   Size:        290     184
 

 Enzymes that do cut:

     AcuI     ApoI     BbsI    BglII   Bpu10I    BsaWI    BspEI    BstYI
   Bsu36I     BtgI     EarI  Eco57MI     FalI    Hin4I  HindIII     MslI
   MspA1I     PfoI    PvuII     SspI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI