(Linear) (Six-base) MAPSORT of: KIBB9435.seq  Check: 2001  from: 1  to: 648



KIBB9435.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     260     648 
   Size:         80     180     388

  Fragments arranged by size: 

                 388     180      80
 
AflIII A'CryG_T

Cuts at:      0     206     648 
   Size:        206     442
 
ApoI r'AATT_y

Cuts at:      0     229     648 
   Size:        229     419
 
AseI AT'TA_AT

Cuts at:      0     619     648 
   Size:        619      29
 
BanI G'GyrC_C

Cuts at:      0     603     648 
   Size:        603      45
 
BbvCI CC'TCA_GC

Cuts at:      0      13     648 
   Size:         13     635
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     297     648 
   Size:        297     351
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     172     648 
   Size:        172     476
 
Bpu10I CC'TnA_GC

Cuts at:      0      13     648 
   Size:         13     635
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     538     648 
   Size:        538     110
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     219     314     648 
   Size:        219      95     334

  Fragments arranged by size: 

                 334     219      95
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     256     648 
   Size:        256     392
 
BsrBI CCG'CTC

Cuts at:      0      23     648 
   Size:         23     625
 
BstYI r'GATC_y

Cuts at:      0      64     153     648 
   Size:         64      89     495

  Fragments arranged by size: 

                 495      89      64
 
Bsu36I CC'TnA_GG

Cuts at:      0     143     648 
   Size:        143     505
 
EarI CTCTTCn'nnn_

Cuts at:      0      30     614     648 
   Size:         30     584      34

  Fragments arranged by size: 

                 584      34      30
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     260     648 
   Size:         80     180     388

  Fragments arranged by size: 

                 388     180      80
 
EcoRI G'AATT_C

Cuts at:      0     229     648 
   Size:        229     419
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     391     648 
   Size:        391     257
 
MslI CAynn'nnrTG

Cuts at:      0      74     648 
   Size:         74     574
 
MspA1I CmG'CkG

Cuts at:      0     516     648 
   Size:        516     132
 
NspI r_CATG'y

Cuts at:      0      94     210     648 
   Size:         94     116     438

  Fragments arranged by size: 

                 438     116      94
 
PciI A'CATG_T

Cuts at:      0     206     648 
   Size:        206     442
 
PstI C_TGCA'G

Cuts at:      0     267     648 
   Size:        267     381
 
PvuII CAG'CTG

Cuts at:      0     516     648 
   Size:        516     132
 
SapI GCTCTTCn'nnn_

Cuts at:      0      30     648 
   Size:         30     618
 
SfcI C'TryA_G

Cuts at:      0     263     648 
   Size:        263     385
 
SspI AAT'ATT

Cuts at:      0     306     648 
   Size:        306     342
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     AseI     BanI    BbvCI     BmrI     BplI
   Bpu10I    BseRI     BsgI    BspMI    BsrBI    BstYI   Bsu36I     EarI
  Eco57MI    EcoRI     FalI     MslI   MspA1I     NspI     PciI     PstI
    PvuII     SapI     SfcI     SspI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI