Restriction map for
pF1KB9433
(Linear) (Six-base) MAPSORT of: KIBB9433.seq Check: 9330 from: 1 to: 816
KIBB9433.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 624 816
Size: 442 182 192
Fragments arranged by size:
442 192 182
AfeI AGC'GCT
Cuts at: 0 226 816
Size: 226 590
AflIII A'CryG_T
Cuts at: 0 519 816
Size: 519 297
AhdI GACnn_n'nnGTC
Cuts at: 0 57 377 816
Size: 57 320 439
Fragments arranged by size:
439 320 57
AlwNI CAG_nnn'CTG
Cuts at: 0 489 531 702 816
Size: 489 42 171 114
Fragments arranged by size:
489 171 114 42
AvaI C'yCGr_G
Cuts at: 0 27 118 816
Size: 27 91 698
Fragments arranged by size:
698 91 27
BanI G'GyrC_C
Cuts at: 0 107 816
Size: 107 709
BbsI GAAGACnn'nnnn_
Cuts at: 0 794 816
Size: 794 22
BglI GCCn_nnn'nGGC
Cuts at: 0 793 816
Size: 793 23
BlpI GC'TnA_GC
Cuts at: 0 124 595 604 816
Size: 124 471 9 212
Fragments arranged by size:
471 212 124 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 735 754 792 816
Size: 735 19 38 24
Fragments arranged by size:
735 38 24 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 816
Size: 469 347
BsaAI yAC'GTr
Cuts at: 0 438 816
Size: 438 378
BsaHI Gr'CG_yC
Cuts at: 0 796 816
Size: 796 20
BsaWI w'CCGG_w
Cuts at: 0 99 816
Size: 99 717
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 22 135 341 553 743 816
Size: 19 3 113 206 212 190 73
Fragments arranged by size:
212 206 190 113 73 19 3
BseYI C'CCAG_C
Cuts at: 0 510 816
Size: 510 306
BsiEI CG_ry'CG
Cuts at: 0 401 816
Size: 401 415
BsmBI CGTCTCn'nnnn_
Cuts at: 0 31 816
Size: 31 785
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 245 710 816
Size: 245 465 106
Fragments arranged by size:
465 245 106
BsrBI CCG'CTC
Cuts at: 0 608 816
Size: 608 208
BsrFI r'CCGG_y
Cuts at: 0 599 655 678 816
Size: 599 56 23 138
Fragments arranged by size:
599 138 56 23
BstAPI GCAn_nnn'nTGC
Cuts at: 0 702 816
Size: 702 114
BstEII G'GTnAC_C
Cuts at: 0 658 816
Size: 658 158
BstYI r'GATC_y
Cuts at: 0 78 816
Size: 78 738
BtgI C'CryG_G
Cuts at: 0 206 816
Size: 206 610
BtsI GCAGTG_nn'
Cuts at: 0 288 529 816
Size: 288 241 287
Fragments arranged by size:
288 287 241
EaeI y'GGCC_r
Cuts at: 0 398 816
Size: 398 418
EagI C'GGCC_G
Cuts at: 0 398 816
Size: 398 418
EarI CTCTTCn'nnn_
Cuts at: 0 29 643 704 816
Size: 29 614 61 112
Fragments arranged by size:
614 112 61 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 91 170 324 816
Size: 91 79 154 492
Fragments arranged by size:
492 154 91 79
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 469 624 816
Size: 442 27 155 192
Fragments arranged by size:
442 192 155 27
EcoO109I rG'GnC_Cy
Cuts at: 0 136 699 816
Size: 136 563 117
Fragments arranged by size:
563 136 117
HaeII r_GCGC'y
Cuts at: 0 228 299 816
Size: 228 71 517
Fragments arranged by size:
517 228 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 363 395 745 777 816
Size: 43 32 288 32 350 32 39
Fragments arranged by size:
350 288 43 39 32 32 32
MluI A'CGCG_T
Cuts at: 0 519 816
Size: 519 297
MspA1I CmG'CkG
Cuts at: 0 208 347 403 816
Size: 208 139 56 413
Fragments arranged by size:
413 208 139 56
NaeI GCC'GGC
Cuts at: 0 601 816
Size: 601 215
NgoMIV G'CCGG_C
Cuts at: 0 599 816
Size: 599 217
NotI GC'GGCC_GC
Cuts at: 0 398 816
Size: 398 418
PfoI T'CCnGG_A
Cuts at: 0 83 816
Size: 83 733
PpuMI rG'GwC_Cy
Cuts at: 0 699 816
Size: 699 117
PstI C_TGCA'G
Cuts at: 0 274 536 816
Size: 274 262 280
Fragments arranged by size:
280 274 262
SacII CC_GC'GG
Cuts at: 0 209 816
Size: 209 607
SapI GCTCTTCn'nnn_
Cuts at: 0 643 816
Size: 643 173
SfcI C'TryA_G
Cuts at: 0 270 532 816
Size: 270 262 284
Fragments arranged by size:
284 270 262
SgrAI Cr'CCGG_yG
Cuts at: 0 655 816
Size: 655 161
SmaI CCC'GGG
Cuts at: 0 29 120 816
Size: 29 91 696
Fragments arranged by size:
696 91 29
XmaI C'CCGG_G
Cuts at: 0 27 118 816
Size: 27 91 698
Fragments arranged by size:
698 91 27
Enzymes that do cut:
AcuI AfeI AflIII AhdI AlwNI AvaI BanI BbsI
BglI BlpI BplI BpmI BsaAI BsaHI BsaWI BseRI
BseYI BsiEI BsmBI BspMI BsrBI BsrFI BstAPI BstEII
BstYI BtgI BtsI EaeI EagI EarI EciI Eco57MI
EcoO109I HaeII Hin4I MluI MspA1I NaeI NgoMIV NotI
PfoI PpuMI PstI SacII SapI SfcI SgrAI SmaI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglII Bme1580I BmgBI BmrI BmtI Bpu10I
BpuEI BsaI BsaBI BsaXI BsgI BsiHKAI BsiWI BsmI
Bsp1286I BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MmeI
MscI MslI NarI NcoI NdeI NheI NruI NsiI
NspI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI