(Linear) (Six-base) MAPSORT of: KIBB9419.seq  Check: 6607  from: 1  to: 1629



KIBB9419.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     119    1040    1343    1629 
   Size:        119     921     303     286

  Fragments arranged by size: 

                 921     303     286     119
 
AflII C'TTAA_G

Cuts at:      0    1078    1629 
   Size:       1078     551
 
AhdI GACnn_n'nnGTC

Cuts at:      0     788    1629 
   Size:        788     841
 
AleI CACnn'nnGTG

Cuts at:      0     112    1629 
   Size:        112    1517
 
AlwNI CAG_nnn'CTG

Cuts at:      0      70    1321    1629 
   Size:         70    1251     308

  Fragments arranged by size: 

                1251     308      70
 
ApoI r'AATT_y

Cuts at:      0    1045    1297    1376    1623    1629 
   Size:       1045     252      79     247       6

  Fragments arranged by size: 

                1045     252     247      79       6
 
AvaI C'yCGr_G

Cuts at:      0     426     818    1629 
   Size:        426     392     811

  Fragments arranged by size: 

                 811     426     392
 
BamHI G'GATC_C

Cuts at:      0     190    1629 
   Size:        190    1439
 
BanI G'GyrC_C

Cuts at:      0     240    1629 
   Size:        240    1389
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     329    1271    1586    1629 
   Size:        329     942     315      43

  Fragments arranged by size: 

                 942     329     315      43
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     655    1456    1629 
   Size:        655     801     173

  Fragments arranged by size: 

                 801     655     173
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1484    1629 
   Size:       1484     145
 
BglII A'GATC_T

Cuts at:      0    1185    1629 
   Size:       1185     444
 
Bme1580I G_kGCm'C

Cuts at:      0    1480    1629 
   Size:       1480     149
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47    1629 
   Size:         47    1582
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     770    1629 
   Size:        770     859
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1313    1629 
   Size:       1313     316
 
BsiHKAI G_wGCw'C

Cuts at:      0     684    1629 
   Size:        684     945
 
Bsp1286I G_dGCh'C

Cuts at:      0     684    1480    1629 
   Size:        684     796     149

  Fragments arranged by size: 

                 796     684     149
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     802    1629 
   Size:        802     827
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1310    1629 
   Size:       1310     319
 
BstYI r'GATC_y

Cuts at:      0     190    1185    1629 
   Size:        190     995     444

  Fragments arranged by size: 

                 995     444     190
 
Bsu36I CC'TnA_GG

Cuts at:      0     195    1481    1629 
   Size:        195    1286     148

  Fragments arranged by size: 

                1286     195     148
 
BtsI GCAGTG_nn'

Cuts at:      0    1589    1629 
   Size:       1589      40
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      47     119    1040    1343    1629 
   Size:         47      72     921     303     286

  Fragments arranged by size: 

                 921     303     286      72      47
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     212    1629 
   Size:        212    1417
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1533    1629 
   Size:       1533      96
 
EcoRI G'AATT_C

Cuts at:      0    1376    1629 
   Size:       1376     253
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     967    1629 
   Size:        967     662
 
HaeII r_GCGC'y

Cuts at:      0     445    1629 
   Size:        445    1184
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     653     685     774     806    1351    1383    1629 
   Size:        653      32      89      32     545      32     246

  Fragments arranged by size: 

                 653     545     246      89      32      32      32
 
HincII GTy'rAC

Cuts at:      0     752    1629 
   Size:        752     877
 
HindIII A'AGCT_T

Cuts at:      0     944    1062    1105    1629 
   Size:        944     118      43     524

  Fragments arranged by size: 

                 944     524     118      43
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     790    1504    1629 
   Size:        790     714     125

  Fragments arranged by size: 

                 790     714     125
 
MslI CAynn'nnrTG

Cuts at:      0     112     731    1629 
   Size:        112     619     898

  Fragments arranged by size: 

                 898     619     112
 
MspA1I CmG'CkG

Cuts at:      0    1116    1629 
   Size:       1116     513
 
NspI r_CATG'y

Cuts at:      0     730    1629 
   Size:        730     899
 
PfoI T'CCnGG_A

Cuts at:      0     522    1629 
   Size:        522    1107
 
PpuMI rG'GwC_Cy

Cuts at:      0    1533    1629 
   Size:       1533      96
 
PstI C_TGCA'G

Cuts at:      0     450     701    1629 
   Size:        450     251     928

  Fragments arranged by size: 

                 928     450     251
 
PvuII CAG'CTG

Cuts at:      0    1116    1629 
   Size:       1116     513
 
ScaI AGT'ACT

Cuts at:      0    1442    1629 
   Size:       1442     187
 
SexAI A'CCwGG_T

Cuts at:      0     924    1629 
   Size:        924     705
 
SfcI C'TryA_G

Cuts at:      0     110     317     404     446     697    1595    1629 
   Size:        110     207      87      42     251     898      34

  Fragments arranged by size: 

                 898     251     207     110      87      42      34
 
SmlI C'TyrA_G

Cuts at:      0     785     818    1078    1629 
   Size:        785      33     260     551

  Fragments arranged by size: 

                 785     551     260      33
 
SpeI A'CTAG_T

Cuts at:      0    1392    1629 
   Size:       1392     237
 
SspI AAT'ATT

Cuts at:      0    1203    1629 
   Size:       1203     426
 
TatI w'GTAC_w

Cuts at:      0      76    1440    1629 
   Size:         76    1364     189

  Fragments arranged by size: 

                1364     189      76
 
XhoI C'TCGA_G

Cuts at:      0     818    1629 
   Size:        818     811
 
XmnI GAAnn'nnTTC

Cuts at:      0     350    1562    1629 
   Size:        350    1212      67

  Fragments arranged by size: 

                1212     350      67
 

 Enzymes that do cut:

     AcuI    AflII     AhdI     AleI    AlwNI     ApoI     AvaI    BamHI
     BanI     BbsI    BciVI     BglI    BglII Bme1580I     BpmI    BpuEI
     BsgI  BsiHKAI Bsp1286I   BstAPI    BstXI    BstYI   Bsu36I     BtsI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I   HincII
  HindIII     MmeI     MslI   MspA1I     NspI     PfoI    PpuMI     PstI
    PvuII     ScaI    SexAI     SfcI     SmlI     SpeI     SspI     TatI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BanII     BbeI    BbvCI     BcgI     BclI    BfrBI     BlpI
    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoRV     FseI     FspI    FspAI     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SphI     SrfI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XmaI     ZraI