Restriction map for
pF1KB9418
(Linear) (Six-base) MAPSORT of: KIBB9418.seq Check: 4983 from: 1 to: 1857
KIBB9418.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 453 1857
Size: 453 1404
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 404 464 914 1566 1646 1742 1857
Size: 227 177 60 450 652 80 96 115
Fragments arranged by size:
652 450 227 177 115 96 80 60
AfeI AGC'GCT
Cuts at: 0 850 1857
Size: 850 1007
AflIII A'CryG_T
Cuts at: 0 111 1857
Size: 111 1746
AleI CACnn'nnGTG
Cuts at: 0 415 1857
Size: 415 1442
AlwNI CAG_nnn'CTG
Cuts at: 0 1381 1857
Size: 1381 476
ApaI G_GGCC'C
Cuts at: 0 1501 1857
Size: 1501 356
ApoI r'AATT_y
Cuts at: 0 37 1044 1233 1857
Size: 37 1007 189 624
Fragments arranged by size:
1007 624 189 37
AvrII C'CTAG_G
Cuts at: 0 1297 1857
Size: 1297 560
BamHI G'GATC_C
Cuts at: 0 906 1857
Size: 906 951
BanII G_rGCy'C
Cuts at: 0 170 1477 1501 1857
Size: 170 1307 24 356
Fragments arranged by size:
1307 356 170 24
BbsI GAAGACnn'nnnn_
Cuts at: 0 920 1857
Size: 920 937
BbvCI CC'TCA_GC
Cuts at: 0 305 1857
Size: 305 1552
BglII A'GATC_T
Cuts at: 0 882 1857
Size: 882 975
Bme1580I G_kGCm'C
Cuts at: 0 1501 1857
Size: 1501 356
BmrI ACTGGGnnnn_n'
Cuts at: 0 400 1857
Size: 400 1457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1857
Size: 637 1220
Bpu10I CC'TnA_GC
Cuts at: 0 305 1857
Size: 305 1552
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1661 1857
Size: 1661 196
BsaI GGTCTCn'nnnn_
Cuts at: 0 778 1857
Size: 778 1079
BsaBI GATnn'nnATC
Cuts at: 0 905 1857
Size: 905 952
BsaWI w'CCGG_w
Cuts at: 0 1148 1857
Size: 1148 709
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1110 1140 1596 1626 1857
Size: 1110 30 456 30 231
Fragments arranged by size:
1110 456 231 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 304 1031 1469 1604 1857
Size: 304 727 438 135 253
Fragments arranged by size:
727 438 304 253 135
BseYI C'CCAG_C
Cuts at: 0 561 692 1139 1857
Size: 561 131 447 718
Fragments arranged by size:
718 561 447 131
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 1577 1857
Size: 1132 445 280
Fragments arranged by size:
1132 445 280
BsiEI CG_ry'CG
Cuts at: 0 985 1857
Size: 985 872
BsiHKAI G_wGCw'C
Cuts at: 0 801 1857
Size: 801 1056
Bsp1286I G_dGCh'C
Cuts at: 0 170 801 1477 1501 1857
Size: 170 631 676 24 356
Fragments arranged by size:
676 631 356 170 24
BspEI T'CCGG_A
Cuts at: 0 1148 1857
Size: 1148 709
BspHI T'CATG_A
Cuts at: 0 267 1857
Size: 267 1590
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 453 1366 1506 1857
Size: 453 913 140 351
Fragments arranged by size:
913 453 351 140
BsrDI GCAATG_nn'
Cuts at: 0 1536 1554 1857
Size: 1536 18 303
Fragments arranged by size:
1536 303 18
BsrFI r'CCGG_y
Cuts at: 0 145 980 1857
Size: 145 835 877
Fragments arranged by size:
877 835 145
BstEII G'GTnAC_C
Cuts at: 0 1602 1857
Size: 1602 255
BstYI r'GATC_y
Cuts at: 0 882 906 933 1068 1365 1384 1857
Size: 882 24 27 135 297 19 473
Fragments arranged by size:
882 473 297 135 27 24 19
BtgI C'CryG_G
Cuts at: 0 788 1857
Size: 788 1069
BtsI GCAGTG_nn'
Cuts at: 0 646 740 1857
Size: 646 94 1117
Fragments arranged by size:
1117 646 94
DraI TTT'AAA
Cuts at: 0 714 867 1857
Size: 714 153 990
Fragments arranged by size:
990 714 153
EarI CTCTTCn'nnn_
Cuts at: 0 158 789 1150 1857
Size: 158 631 361 707
Fragments arranged by size:
707 631 361 158
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 404 464 637 914 1566 1646 1742
Size: 227 177 60 173 277 652 80 96
Cuts at: 1742 1857
Size: 115
Fragments arranged by size:
652 277 227 177 173 115 96 80
60
EcoO109I rG'GnC_Cy
Cuts at: 0 1498 1518 1857
Size: 1498 20 339
Fragments arranged by size:
1498 339 20
EcoRI G'AATT_C
Cuts at: 0 1044 1857
Size: 1044 813
HaeII r_GCGC'y
Cuts at: 0 852 1857
Size: 852 1005
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1596 1628 1857
Size: 1596 32 229
Fragments arranged by size:
1596 229 32
HindIII A'AGCT_T
Cuts at: 0 257 1857
Size: 257 1600
MfeI C'AATT_G
Cuts at: 0 284 1487 1857
Size: 284 1203 370
Fragments arranged by size:
1203 370 284
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 1294 1857
Size: 39 1255 563
Fragments arranged by size:
1255 563 39
MslI CAynn'nnrTG
Cuts at: 0 415 1857
Size: 415 1442
MspA1I CmG'CkG
Cuts at: 0 1629 1857
Size: 1629 228
NcoI C'CATG_G
Cuts at: 0 788 1857
Size: 788 1069
NspI r_CATG'y
Cuts at: 0 115 1857
Size: 115 1742
PciI A'CATG_T
Cuts at: 0 111 1857
Size: 111 1746
PflMI CCAn_nnn'nTGG
Cuts at: 0 746 1857
Size: 746 1111
PfoI T'CCnGG_A
Cuts at: 0 653 1857
Size: 653 1204
PpuMI rG'GwC_Cy
Cuts at: 0 1518 1857
Size: 1518 339
PspOMI G'GGCC_C
Cuts at: 0 1497 1857
Size: 1497 360
PstI C_TGCA'G
Cuts at: 0 1460 1857
Size: 1460 397
PvuII CAG'CTG
Cuts at: 0 1629 1857
Size: 1629 228
SapI GCTCTTCn'nnn_
Cuts at: 0 158 789 1150 1857
Size: 158 631 361 707
Fragments arranged by size:
707 631 361 158
ScaI AGT'ACT
Cuts at: 0 1436 1857
Size: 1436 421
SfcI C'TryA_G
Cuts at: 0 1025 1456 1857
Size: 1025 431 401
Fragments arranged by size:
1025 431 401
SmlI C'TyrA_G
Cuts at: 0 1676 1857
Size: 1676 181
StyI C'CwwG_G
Cuts at: 0 788 1297 1857
Size: 788 509 560
Fragments arranged by size:
788 560 509
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 427 1655 1857
Size: 427 1228 202
Fragments arranged by size:
1228 427 202
TatI w'GTAC_w
Cuts at: 0 1164 1173 1434 1857
Size: 1164 9 261 423
Fragments arranged by size:
1164 423 261 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1110 1142 1857
Size: 1110 32 715
Fragments arranged by size:
1110 715 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1071 1857
Size: 1071 786
XmnI GAAnn'nnTTC
Cuts at: 0 113 1857
Size: 113 1744
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlwNI ApaI ApoI
AvrII BamHI BanII BbsI BbvCI BglII Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrDI BsrFI BstEII BstYI BtgI BtsI DraI EarI
Eco57MI EcoO109I EcoRI HaeII Hin4I HindIII MfeI MmeI
MslI MspA1I NcoI NspI PciI PflMI PfoI PpuMI
PspOMI PstI PvuII SapI ScaI SfcI SmlI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AlfI
AloI ApaLI AscI AseI AsiSI AvaI BaeI BanI
BbeI BcgI BciVI BclI BfrBI BglI BlpI BmgBI
BmtI BplI BsaAI BsaHI BsiWI BsmI BsmBI BsrBI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HincII
HpaI KasI KpnI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmaI ZraI