Restriction map for
pF1KB9417
(Linear) (Six-base) MAPSORT of: KIBB9417.seq Check: 1219 from: 1 to: 2655
KIBB9417.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AccI GT'mk_AC
Cuts at: 0 2147 2655
Size: 2147 508
Acc65I G'GTAC_C
Cuts at: 0 1084 1097 1463 2655
Size: 1084 13 366 1192
Fragments arranged by size:
1192 1084 366 13
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 1520 1558 2296 2413 2655
Size: 116 1404 38 738 117 242
Fragments arranged by size:
1404 738 242 117 116 38
AflIII A'CryG_T
Cuts at: 0 2021 2655
Size: 2021 634
AhdI GACnn_n'nnGTC
Cuts at: 0 2569 2655
Size: 2569 86
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 583 615 2655
Size: 583 32 2040
Fragments arranged by size:
2040 583 32
AlwNI CAG_nnn'CTG
Cuts at: 0 225 1819 2003 2655
Size: 225 1594 184 652
Fragments arranged by size:
1594 652 225 184
ApaI G_GGCC'C
Cuts at: 0 949 1006 2655
Size: 949 57 1649
Fragments arranged by size:
1649 949 57
ApoI r'AATT_y
Cuts at: 0 1744 2655
Size: 1744 911
AvaI C'yCGr_G
Cuts at: 0 140 193 1067 1882 2655
Size: 140 53 874 815 773
Fragments arranged by size:
874 815 773 140 53
BanI G'GyrC_C
Cuts at: 0 11 136 154 255 1071 1084 1097 1277
Size: 11 125 18 101 816 13 13 180
Cuts at: 1277 1463 2650 2655
Size: 186 1187 5
Fragments arranged by size:
1187 816 186 180 125 101 18 13
13 11 5
BanII G_rGCy'C
Cuts at: 0 191 233 434 503 845 949 1006 1067
Size: 191 42 201 69 342 104 57 61
Cuts at: 1067 2051 2623 2655
Size: 984 572 32
Fragments arranged by size:
984 572 342 201 191 104 69 61
57 42 32
BbeI G_GCGC'C
Cuts at: 0 1075 1281 2655
Size: 1075 206 1374
Fragments arranged by size:
1374 1075 206
BbsI GAAGACnn'nnnn_
Cuts at: 0 443 1597 1678 2655
Size: 443 1154 81 977
Fragments arranged by size:
1154 977 443 81
BbvCI CC'TCA_GC
Cuts at: 0 2546 2655
Size: 2546 109
BglI GCCn_nnn'nGGC
Cuts at: 0 749 2655
Size: 749 1906
BglII A'GATC_T
Cuts at: 0 2060 2655
Size: 2060 595
BlpI GC'TnA_GC
Cuts at: 0 2603 2655
Size: 2603 52
Bme1580I G_kGCm'C
Cuts at: 0 16 88 141 157 260 869 949 998
Size: 16 72 53 16 103 609 80 49
Cuts at: 998 1006 2655
Size: 8 1649
Fragments arranged by size:
1649 609 103 80 72 53 49 16
16 8
BmrI ACTGGGnnnn_n'
Cuts at: 0 1252 1276 2655
Size: 1252 24 1379
Fragments arranged by size:
1379 1252 24
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 247 424 445 505 533 722 742
Size: 157 90 177 21 60 28 189 20
Cuts at: 742 1192 1269 1288 1729 2495 2655
Size: 450 77 19 441 766 160
Fragments arranged by size:
766 450 441 189 177 160 157 90
77 60 28 21 20 19
Bpu10I CC'TnA_GC
Cuts at: 0 472 485 745 1153 2546 2655
Size: 472 13 260 408 1393 109
Fragments arranged by size:
1393 472 408 260 109 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1642 2655
Size: 1642 1013
BsaI GGTCTCn'nnnn_
Cuts at: 0 705 1399 1844 2655
Size: 705 694 445 811
Fragments arranged by size:
811 705 694 445
BsaBI GATnn'nnATC
Cuts at: 0 808 1687 2655
Size: 808 879 968
Fragments arranged by size:
968 879 808
BsaHI Gr'CG_yC
Cuts at: 0 982 1072 1278 2655
Size: 982 90 206 1377
Fragments arranged by size:
1377 982 206 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 503 533 685 715 1331 1361 2655
Size: 503 30 152 30 616 30 1294
Fragments arranged by size:
1294 616 503 152 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 442 853 1858 2501 2655
Size: 442 411 1005 643 154
Fragments arranged by size:
1005 643 442 411 154
BseYI C'CCAG_C
Cuts at: 0 62 383 563 674 1234 1812 2327 2504
Size: 62 321 180 111 560 578 515 177
Cuts at: 2504 2625 2655
Size: 121 30
Fragments arranged by size:
578 560 515 321 180 177 121 111
62 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 756 1677 2655
Size: 756 921 978
Fragments arranged by size:
978 921 756
BsiHKAI G_wGCw'C
Cuts at: 0 233 671 2655
Size: 233 438 1984
Fragments arranged by size:
1984 438 233
BsmBI CGTCTCn'nnnn_
Cuts at: 0 973 2655
Size: 973 1682
Bsp1286I G_dGCh'C
Cuts at: 0 16 88 141 157 191 233 260 434
Size: 16 72 53 16 34 42 27 174
Cuts at: 434 503 671 845 869 949 998 1006 1067
Size: 69 168 174 24 80 49 8 61
Cuts at: 1067 2051 2623 2655
Size: 984 572 32
Fragments arranged by size:
984 572 174 174 168 80 72 69
61 53 49 42 34 32 27 24
16 16 8
BspHI T'CATG_A
Cuts at: 0 1763 1838 2655
Size: 1763 75 817
Fragments arranged by size:
1763 817 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1910 2655
Size: 1910 745
BsrBI CCG'CTC
Cuts at: 0 749 2602 2655
Size: 749 1853 53
Fragments arranged by size:
1853 749 53
BsrFI r'CCGG_y
Cuts at: 0 1100 1391 1568 2348 2655
Size: 1100 291 177 780 307
Fragments arranged by size:
1100 780 307 291 177
BssSI C'ACGA_G
Cuts at: 0 1311 1695 2655
Size: 1311 384 960
Fragments arranged by size:
1311 960 384
BstAPI GCAn_nnn'nTGC
Cuts at: 0 786 2655
Size: 786 1869
BstEII G'GTnAC_C
Cuts at: 0 636 1164 2655
Size: 636 528 1491
Fragments arranged by size:
1491 636 528
BstXI CCAn_nnnn'nTGG
Cuts at: 0 720 1950 2114 2560 2655
Size: 720 1230 164 446 95
Fragments arranged by size:
1230 720 446 164 95
BstYI r'GATC_y
Cuts at: 0 221 2060 2655
Size: 221 1839 595
Fragments arranged by size:
1839 595 221
Bsu36I CC'TnA_GG
Cuts at: 0 2516 2655
Size: 2516 139
BtgI C'CryG_G
Cuts at: 0 503 542 713 2655
Size: 503 39 171 1942
Fragments arranged by size:
1942 503 171 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1923 2177 2655
Size: 1923 254 478
Fragments arranged by size:
1923 478 254
BtsI GCAGTG_nn'
Cuts at: 0 768 1226 2537 2655
Size: 768 458 1311 118
Fragments arranged by size:
1311 768 458 118
DraIII CAC_nnn'GTG
Cuts at: 0 1578 2655
Size: 1578 1077
DrdI GACnn_nn'nnGTC
Cuts at: 0 378 2655
Size: 378 2277
EaeI y'GGCC_r
Cuts at: 0 449 539 1349 1994 2350 2655
Size: 449 90 810 645 356 305
Fragments arranged by size:
810 645 449 356 305 90
EarI CTCTTCn'nnn_
Cuts at: 0 1384 1526 2655
Size: 1384 142 1129
Fragments arranged by size:
1384 1129 142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2053 2655
Size: 2053 602
EcoICRI GAG'CTC
Cuts at: 0 231 2655
Size: 231 2424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 157 247 424 445 505 533 722
Size: 116 41 90 177 21 60 28 189
Cuts at: 722 742 1192 1269 1288 1520 1558 1729 2296
Size: 20 450 77 19 232 38 171 567
Cuts at: 2296 2413 2495 2655
Size: 117 82 160
Fragments arranged by size:
567 450 232 189 177 171 160 117
116 90 82 77 60 41 38 28
21 20 19
EcoNI CCTnn'n_nnAGG
Cuts at: 0 265 2655
Size: 265 2390
EcoO109I rG'GnC_Cy
Cuts at: 0 30 490 945 946 1003 1986 2655
Size: 30 460 455 1 57 983 669
Fragments arranged by size:
983 669 460 455 57 30 1
FspI TGC'GCA
Cuts at: 0 1474 2655
Size: 1474 1181
HaeII r_GCGC'y
Cuts at: 0 55 1075 1281 2655
Size: 55 1020 206 1374
Fragments arranged by size:
1374 1020 206 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2008 2040 2155 2187 2555 2587 2605 2637
Size: 2008 32 115 32 368 32 18 32
Cuts at: 2637 2655
Size: 18
Fragments arranged by size:
2008 368 115 32 32 32 32 18
18
HincII GTy'rAC
Cuts at: 0 2439 2655
Size: 2439 216
KasI G'GCGC_C
Cuts at: 0 1071 1277 2655
Size: 1071 206 1378
Fragments arranged by size:
1378 1071 206
KpnI G_GTAC'C
Cuts at: 0 1088 1101 1467 2655
Size: 1088 13 366 1188
Fragments arranged by size:
1188 1088 366 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 1471 2655
Size: 353 1118 1184
Fragments arranged by size:
1184 1118 353
MscI TGG'CCA
Cuts at: 0 541 2655
Size: 541 2114
MslI CAynn'nnrTG
Cuts at: 0 664 922 1912 2026 2655
Size: 664 258 990 114 629
Fragments arranged by size:
990 664 629 258 114
MspA1I CmG'CkG
Cuts at: 0 36 1354 2655
Size: 36 1318 1301
Fragments arranged by size:
1318 1301 36
NarI GG'CG_CC
Cuts at: 0 1072 1278 2655
Size: 1072 206 1377
Fragments arranged by size:
1377 1072 206
NspI r_CATG'y
Cuts at: 0 580 932 2010 2025 2655
Size: 580 352 1078 15 630
Fragments arranged by size:
1078 630 580 352 15
PasI CC'CwG_GG
Cuts at: 0 80 121 261 999 1584 2635 2655
Size: 80 41 140 738 585 1051 20
Fragments arranged by size:
1051 738 585 140 80 41 20
PciI A'CATG_T
Cuts at: 0 2021 2655
Size: 2021 634
PflMI CCAn_nnn'nTGG
Cuts at: 0 351 2655
Size: 351 2304
PfoI T'CCnGG_A
Cuts at: 0 224 422 521 955 2655
Size: 224 198 99 434 1700
Fragments arranged by size:
1700 434 224 198 99
PpuMI rG'GwC_Cy
Cuts at: 0 30 490 1986 2655
Size: 30 460 1496 669
Fragments arranged by size:
1496 669 460 30
PspOMI G'GGCC_C
Cuts at: 0 945 1002 2655
Size: 945 57 1653
Fragments arranged by size:
1653 945 57
PstI C_TGCA'G
Cuts at: 0 323 782 1082 1181 2484 2655
Size: 323 459 300 99 1303 171
Fragments arranged by size:
1303 459 323 300 171 99
SacI G_AGCT'C
Cuts at: 0 233 2655
Size: 233 2422
SanDI GG'GwC_CC
Cuts at: 0 30 490 2655
Size: 30 460 2165
Fragments arranged by size:
2165 460 30
SapI GCTCTTCn'nnn_
Cuts at: 0 1526 2655
Size: 1526 1129
SbfI CC_TGCA'GG
Cuts at: 0 782 1082 2655
Size: 782 300 1573
Fragments arranged by size:
1573 782 300
SexAI A'CCwGG_T
Cuts at: 0 695 2655
Size: 695 1960
SfcI C'TryA_G
Cuts at: 0 319 778 1078 1177 1482 2480 2526 2655
Size: 319 459 300 99 305 998 46 129
Fragments arranged by size:
998 459 319 305 300 129 99 46
SfoI GGC'GCC
Cuts at: 0 1073 1279 2655
Size: 1073 206 1376
Fragments arranged by size:
1376 1073 206
SgrAI Cr'CCGG_yG
Cuts at: 0 1391 2655
Size: 1391 1264
SmaI CCC'GGG
Cuts at: 0 142 1069 2655
Size: 142 927 1586
Fragments arranged by size:
1586 927 142
SmlI C'TyrA_G
Cuts at: 0 1657 2655
Size: 1657 998
SphI G_CATG'C
Cuts at: 0 932 2010 2655
Size: 932 1078 645
Fragments arranged by size:
1078 932 645
StuI AGG'CCT
Cuts at: 0 743 1045 1273 2398 2655
Size: 743 302 228 1125 257
Fragments arranged by size:
1125 743 302 257 228
StyI C'CwwG_G
Cuts at: 0 629 2655
Size: 629 2026
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 253 259 2655
Size: 253 6 2396
Fragments arranged by size:
2396 253 6
TatI w'GTAC_w
Cuts at: 0 1329 2655
Size: 1329 1326
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 685 717 1331 1363 2655
Size: 685 32 614 32 1292
Fragments arranged by size:
1292 685 614 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2143 2655
Size: 2143 512
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1659 2655
Size: 1659 996
XmaI C'CCGG_G
Cuts at: 0 140 1067 2655
Size: 140 927 1588
Fragments arranged by size:
1588 927 140
Enzymes that do cut:
AccI Acc65I AcuI AflIII AhdI AloI AlwNI ApaI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BglI
BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrFI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII KasI KpnI MmeI MscI MslI
MspA1I NarI NspI PasI PciI PflMI PfoI PpuMI
PspOMI PstI SacI SanDI SapI SbfI SexAI SfcI
SfoI SgrAI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AleI AlfI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BmgBI BmtI BplI BsaAI BsaWI
BsiEI BsiWI BsmI BspEI BsrDI BsrGI BssHII BstBI
BstZ17I ClaI CspCI DraI EagI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI MfeI MluI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacII SalI ScaI SfiI SgfI SnaBI SpeI
SrfI SspI SwaI XbaI XhoI XmnI ZraI