Restriction map for
pF1KB9409
(Linear) (Six-base) MAPSORT of: KIBB9409.seq Check: 2649 from: 1 to: 867
KIBB9409.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AfeI AGC'GCT
Cuts at: 0 106 867
Size: 106 761
AleI CACnn'nnGTG
Cuts at: 0 715 867
Size: 715 152
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 58 867
Size: 58 809
AlwNI CAG_nnn'CTG
Cuts at: 0 87 405 867
Size: 87 318 462
Fragments arranged by size:
462 318 87
BamHI G'GATC_C
Cuts at: 0 802 867
Size: 802 65
BanII G_rGCy'C
Cuts at: 0 641 664 867
Size: 641 23 203
Fragments arranged by size:
641 203 23
BbsI GAAGACnn'nnnn_
Cuts at: 0 219 867
Size: 219 648
BmgBI CAC'GTC
Cuts at: 0 717 849 867
Size: 717 132 18
Fragments arranged by size:
717 132 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 430 625 867
Size: 211 219 195 242
Fragments arranged by size:
242 219 211 195
BsaI GGTCTCn'nnnn_
Cuts at: 0 334 867
Size: 334 533
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 352 382 505 535 867
Size: 25 327 30 123 30 332
Fragments arranged by size:
332 327 123 30 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 455 751 867
Size: 115 340 296 116
Fragments arranged by size:
340 296 116 115
BseYI C'CCAG_C
Cuts at: 0 265 429 503 867
Size: 265 164 74 364
Fragments arranged by size:
364 265 164 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 867
Size: 160 707
BsiEI CG_ry'CG
Cuts at: 0 72 867
Size: 72 795
BsiHKAI G_wGCw'C
Cuts at: 0 124 485 664 761 867
Size: 124 361 179 97 106
Fragments arranged by size:
361 179 124 106 97
BsmBI CGTCTCn'nnnn_
Cuts at: 0 59 867
Size: 59 808
Bsp1286I G_dGCh'C
Cuts at: 0 124 485 641 664 761 867
Size: 124 361 156 23 97 106
Fragments arranged by size:
361 156 124 106 97 23
BspHI T'CATG_A
Cuts at: 0 468 867
Size: 468 399
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 65 95 867
Size: 65 30 772
Fragments arranged by size:
772 65 30
BsrDI GCAATG_nn'
Cuts at: 0 551 867
Size: 551 316
BssSI C'ACGA_G
Cuts at: 0 16 867
Size: 16 851
BstAPI GCAn_nnn'nTGC
Cuts at: 0 87 489 867
Size: 87 402 378
Fragments arranged by size:
402 378 87
BstYI r'GATC_y
Cuts at: 0 802 867
Size: 802 65
Bsu36I CC'TnA_GG
Cuts at: 0 398 867
Size: 398 469
BtgI C'CryG_G
Cuts at: 0 356 867
Size: 356 511
BtsI GCAGTG_nn'
Cuts at: 0 124 867
Size: 124 743
DraIII CAC_nnn'GTG
Cuts at: 0 21 867
Size: 21 846
DrdI GACnn_nn'nnGTC
Cuts at: 0 73 282 867
Size: 73 209 585
Fragments arranged by size:
585 209 73
EaeI y'GGCC_r
Cuts at: 0 176 236 867
Size: 176 60 631
Fragments arranged by size:
631 176 60
EarI CTCTTCn'nnn_
Cuts at: 0 215 867
Size: 215 652
EcoICRI GAG'CTC
Cuts at: 0 662 867
Size: 662 205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 430 625 867
Size: 211 219 195 242
Fragments arranged by size:
242 219 211 195
EcoO109I rG'GnC_Cy
Cuts at: 0 270 867
Size: 270 597
EcoRV GAT'ATC
Cuts at: 0 858 867
Size: 858 9
FspI TGC'GCA
Cuts at: 0 166 493 867
Size: 166 327 374
Fragments arranged by size:
374 327 166
HaeII r_GCGC'y
Cuts at: 0 108 867
Size: 108 759
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 505 537 614 646 867
Size: 505 32 77 32 221
Fragments arranged by size:
505 221 77 32 32
HindIII A'AGCT_T
Cuts at: 0 731 867
Size: 731 136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 791 867
Size: 177 614 76
Fragments arranged by size:
614 177 76
MscI TGG'CCA
Cuts at: 0 178 238 867
Size: 178 60 629
Fragments arranged by size:
629 178 60
MslI CAynn'nnrTG
Cuts at: 0 184 715 867
Size: 184 531 152
Fragments arranged by size:
531 184 152
MspA1I CmG'CkG
Cuts at: 0 351 358 457 510 867
Size: 351 7 99 53 357
Fragments arranged by size:
357 351 99 53 7
NspI r_CATG'y
Cuts at: 0 165 318 541 867
Size: 165 153 223 326
Fragments arranged by size:
326 223 165 153
PflMI CCAn_nnn'nTGG
Cuts at: 0 21 867
Size: 21 846
PpuMI rG'GwC_Cy
Cuts at: 0 270 867
Size: 270 597
PstI C_TGCA'G
Cuts at: 0 83 776 867
Size: 83 693 91
Fragments arranged by size:
693 91 83
PvuII CAG'CTG
Cuts at: 0 457 867
Size: 457 410
RsrII CG'GwC_CG
Cuts at: 0 30 867
Size: 30 837
SacI G_AGCT'C
Cuts at: 0 664 867
Size: 664 203
SacII CC_GC'GG
Cuts at: 0 359 867
Size: 359 508
SanDI GG'GwC_CC
Cuts at: 0 270 867
Size: 270 597
SfcI C'TryA_G
Cuts at: 0 79 772 842 867
Size: 79 693 70 25
Fragments arranged by size:
693 79 70 25
SphI G_CATG'C
Cuts at: 0 541 867
Size: 541 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 292 867
Size: 292 575
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 196 228 352 384 867
Size: 27 169 32 124 32 483
Fragments arranged by size:
483 169 124 32 32 27
Tth111I GACn'n_nGTC
Cuts at: 0 28 73 867
Size: 28 45 794
Fragments arranged by size:
794 45 28
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 186 246 714 867
Size: 186 60 468 153
Fragments arranged by size:
468 186 153 60
Enzymes that do cut:
AfeI AleI AlfI AlwNI BamHI BanII BbsI BmgBI
BpmI BsaI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrDI BssSI BstAPI BstYI
Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRV FspI HaeII Hin4I HindIII MmeI
MscI MslI MspA1I NspI PflMI PpuMI PstI PvuII
RsrII SacI SacII SanDI SfcI SphI TaqII TstI
Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsiWI BsmI BspEI BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI EagI
EciI EcoNI EcoRI FalI FseI FspAI HincII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI StyI SwaI TatI XbaI XhoI XmaI
XmnI ZraI