Restriction map for
pF1KB9407
(Linear) (Six-base) MAPSORT of: KIBB9407.seq Check: 1222 from: 1 to: 3774
KIBB9407.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2328 2445 3774
Size: 2328 117 1329
Fragments arranged by size:
2328 1329 117
AccI GT'mk_AC
Cuts at: 0 649 1136 3774
Size: 649 487 2638
Fragments arranged by size:
2638 649 487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 1348 3715 3774
Size: 613 735 2367 59
Fragments arranged by size:
2367 735 613 59
AleI CACnn'nnGTG
Cuts at: 0 2299 2983 3774
Size: 2299 684 791
Fragments arranged by size:
2299 791 684
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2317 2753 3001 3774
Size: 2317 436 248 773
Fragments arranged by size:
2317 773 436 248
ApaI G_GGCC'C
Cuts at: 0 184 2394 3774
Size: 184 2210 1380
Fragments arranged by size:
2210 1380 184
ApoI r'AATT_y
Cuts at: 0 2487 3599 3774
Size: 2487 1112 175
Fragments arranged by size:
2487 1112 175
AseI AT'TA_AT
Cuts at: 0 2668 3774
Size: 2668 1106
AvaI C'yCGr_G
Cuts at: 0 183 508 2308 2916 3644 3774
Size: 183 325 1800 608 728 130
Fragments arranged by size:
1800 728 608 325 183 130
BanI G'GyrC_C
Cuts at: 0 538 998 1639 2033 2790 2811 3774
Size: 538 460 641 394 757 21 963
Fragments arranged by size:
963 757 641 538 460 394 21
BanII G_rGCy'C
Cuts at: 0 184 386 2197 2394 2917 3142 3774
Size: 184 202 1811 197 523 225 632
Fragments arranged by size:
1811 632 523 225 202 197 184
BbsI GAAGACnn'nnnn_
Cuts at: 0 1115 1159 3774
Size: 1115 44 2615
Fragments arranged by size:
2615 1115 44
BclI T'GATC_A
Cuts at: 0 375 602 3774
Size: 375 227 3172
Fragments arranged by size:
3172 375 227
BfrBI ATG'CAT
Cuts at: 0 3094 3774
Size: 3094 680
BglII A'GATC_T
Cuts at: 0 1616 3774
Size: 1616 2158
BlpI GC'TnA_GC
Cuts at: 0 3546 3774
Size: 3546 228
Bme1580I G_kGCm'C
Cuts at: 0 184 2394 2795 2816 3774
Size: 184 2210 401 21 958
Fragments arranged by size:
2210 958 401 184 21
BmgBI CAC'GTC
Cuts at: 0 255 1851 2754 3000 3774
Size: 255 1596 903 246 774
Fragments arranged by size:
1596 903 774 255 246
BmrI ACTGGGnnnn_n'
Cuts at: 0 927 2388 2632 3774
Size: 927 1461 244 1142
Fragments arranged by size:
1461 1142 927 244
BmtI G_CTAG'C
Cuts at: 0 1787 3774
Size: 1787 1987
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 539 2832 3774
Size: 539 2293 942
Fragments arranged by size:
2293 942 539
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 370 1477 2321 2559 2678 3774
Size: 232 138 1107 844 238 119 1096
Fragments arranged by size:
1107 1096 844 238 232 138 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1222 1589 3774
Size: 1222 367 2185
Fragments arranged by size:
2185 1222 367
BsaI GGTCTCn'nnnn_
Cuts at: 0 374 1759 3167 3613 3774
Size: 374 1385 1408 446 161
Fragments arranged by size:
1408 1385 446 374 161
BsaHI Gr'CG_yC
Cuts at: 0 2686 3774
Size: 2686 1088
BsaWI w'CCGG_w
Cuts at: 0 3176 3774
Size: 3176 598
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 109 139 426 456 2288 2318 3774
Size: 109 30 287 30 1832 30 1456
Fragments arranged by size:
1832 1456 287 109 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 730 733 793 1075 2183 2817 3491 3521
Size: 730 3 60 282 1108 634 674 30
Cuts at: 3521 3774
Size: 253
Fragments arranged by size:
1108 730 674 634 282 253 60 30
3
BseYI C'CCAG_C
Cuts at: 0 81 96 189 258 1842 3774
Size: 81 15 93 69 1584 1932
Fragments arranged by size:
1932 1584 93 81 69 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 2107 2254 3007 3774
Size: 238 1869 147 753 767
Fragments arranged by size:
1869 767 753 238 147
BsiEI CG_ry'CG
Cuts at: 0 149 2646 3774
Size: 149 2497 1128
Fragments arranged by size:
2497 1128 149
BsiHKAI G_wGCw'C
Cuts at: 0 113 386 785 2306 2917 3774
Size: 113 273 399 1521 611 857
Fragments arranged by size:
1521 857 611 399 273 113
BsiWI C'GTAC_G
Cuts at: 0 125 3774
Size: 125 3649
BsmI GAATG_Cn'
Cuts at: 0 55 3303 3639 3774
Size: 55 3248 336 135
Fragments arranged by size:
3248 336 135 55
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3698 3774
Size: 3698 76
Bsp1286I G_dGCh'C
Cuts at: 0 113 184 386 785 2197 2306 2394 2795
Size: 113 71 202 399 1412 109 88 401
Cuts at: 2795 2816 2917 3142 3774
Size: 21 101 225 632
Fragments arranged by size:
1412 632 401 399 225 202 113 109
101 88 71 21
BspHI T'CATG_A
Cuts at: 0 378 3774
Size: 378 3396
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 2328 2445 3774
Size: 341 1987 117 1329
Fragments arranged by size:
1987 1329 341 117
BsrBI CCG'CTC
Cuts at: 0 136 3774
Size: 136 3638
BsrDI GCAATG_nn'
Cuts at: 0 1675 3096 3774
Size: 1675 1421 678
Fragments arranged by size:
1675 1421 678
BsrFI r'CCGG_y
Cuts at: 0 2850 3774
Size: 2850 924
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3091 3774
Size: 3091 683
BstYI r'GATC_y
Cuts at: 0 1616 2055 2373 3005 3179 3774
Size: 1616 439 318 632 174 595
Fragments arranged by size:
1616 632 595 439 318 174
Bsu36I CC'TnA_GG
Cuts at: 0 2135 2650 3774
Size: 2135 515 1124
Fragments arranged by size:
2135 1124 515
BtgI C'CryG_G
Cuts at: 0 2981 3774
Size: 2981 793
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 813 3774
Size: 813 2961
BtsI GCAGTG_nn'
Cuts at: 0 2952 3774
Size: 2952 822
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2472 2507 3774
Size: 2472 35 1267
Fragments arranged by size:
2472 1267 35
DraI TTT'AAA
Cuts at: 0 2382 3774
Size: 2382 1392
DraIII CAC_nnn'GTG
Cuts at: 0 2626 3774
Size: 2626 1148
EaeI y'GGCC_r
Cuts at: 0 1998 2358 2643 3672 3774
Size: 1998 360 285 1029 102
Fragments arranged by size:
1998 1029 360 285 102
EagI C'GGCC_G
Cuts at: 0 2643 3774
Size: 2643 1131
EarI CTCTTCn'nnn_
Cuts at: 0 530 1022 2185 2861 3774
Size: 530 492 1163 676 913
Fragments arranged by size:
1163 913 676 530 492
EcoICRI GAG'CTC
Cuts at: 0 384 2915 3774
Size: 384 2531 859
Fragments arranged by size:
2531 859 384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 370 613 1348 1477 2321 2559 2678
Size: 232 138 243 735 129 844 238 119
Cuts at: 2678 3715 3774
Size: 1037 59
Fragments arranged by size:
1037 844 735 243 238 232 138 129
119 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3241 3774
Size: 3241 533
EcoO109I rG'GnC_Cy
Cuts at: 0 67 180 330 901 2091 3368 3774
Size: 67 113 150 571 1190 1277 406
Fragments arranged by size:
1277 1190 571 406 150 113 67
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 516 1378 3774
Size: 516 862 2396
Fragments arranged by size:
2396 862 516
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 329 361 1272 1304 3164 3188 3196 3220
Size: 329 32 911 32 1860 24 8 24
Cuts at: 3220 3277 3309 3774
Size: 57 32 465
Fragments arranged by size:
1860 911 465 329 57 32 32 32
24 24 8
HincII GTy'rAC
Cuts at: 0 650 2022 3774
Size: 650 1372 1752
Fragments arranged by size:
1752 1372 650
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 111 1446 3103 3774
Size: 111 1335 1657 671
Fragments arranged by size:
1657 1335 671 111
MscI TGG'CCA
Cuts at: 0 2000 2360 3774
Size: 2000 360 1414
Fragments arranged by size:
2000 1414 360
MslI CAynn'nnrTG
Cuts at: 0 1598 2299 2983 3774
Size: 1598 701 684 791
Fragments arranged by size:
1598 791 701 684
MspA1I CmG'CkG
Cuts at: 0 157 275 1846 2924 2936 2966 3152 3774
Size: 157 118 1571 1078 12 30 186 622
Fragments arranged by size:
1571 1078 622 186 157 118 30 12
NaeI GCC'GGC
Cuts at: 0 2852 3774
Size: 2852 922
NgoMIV G'CCGG_C
Cuts at: 0 2850 3774
Size: 2850 924
NheI G'CTAG_C
Cuts at: 0 1783 3774
Size: 1783 1991
NsiI A_TGCA'T
Cuts at: 0 3096 3774
Size: 3096 678
NspI r_CATG'y
Cuts at: 0 1603 2409 3098 3774
Size: 1603 806 689 676
Fragments arranged by size:
1603 806 689 676
PasI CC'CwG_GG
Cuts at: 0 72 3774
Size: 72 3702
PflMI CCAn_nnn'nTGG
Cuts at: 0 592 2112 2695 2715 3724 3774
Size: 592 1520 583 20 1009 50
Fragments arranged by size:
1520 1009 592 583 50 20
PfoI T'CCnGG_A
Cuts at: 0 2398 3774
Size: 2398 1376
PpuMI rG'GwC_Cy
Cuts at: 0 67 330 901 3368 3774
Size: 67 263 571 2467 406
Fragments arranged by size:
2467 571 406 263 67
PshAI GACnn'nnGTC
Cuts at: 0 2424 3774
Size: 2424 1350
PspOMI G'GGCC_C
Cuts at: 0 180 2390 3774
Size: 180 2210 1384
Fragments arranged by size:
2210 1384 180
PspXI vC'TCGA_Gb
Cuts at: 0 508 3774
Size: 508 3266
PstI C_TGCA'G
Cuts at: 0 888 2325 3774
Size: 888 1437 1449
Fragments arranged by size:
1449 1437 888
PvuII CAG'CTG
Cuts at: 0 275 3774
Size: 275 3499
SacI G_AGCT'C
Cuts at: 0 386 2917 3774
Size: 386 2531 857
Fragments arranged by size:
2531 857 386
SalI G'TCGA_C
Cuts at: 0 648 3774
Size: 648 3126
SanDI GG'GwC_CC
Cuts at: 0 67 3368 3774
Size: 67 3301 406
Fragments arranged by size:
3301 406 67
SapI GCTCTTCn'nnn_
Cuts at: 0 2185 3774
Size: 2185 1589
SfcI C'TryA_G
Cuts at: 0 884 2321 3650 3774
Size: 884 1437 1329 124
Fragments arranged by size:
1437 1329 884 124
SmlI C'TyrA_G
Cuts at: 0 508 1201 1604 3644 3774
Size: 508 693 403 2040 130
Fragments arranged by size:
2040 693 508 403 130
StuI AGG'CCT
Cuts at: 0 2042 3774
Size: 2042 1732
StyI C'CwwG_G
Cuts at: 0 2029 3482 3774
Size: 2029 1453 292
Fragments arranged by size:
2029 1453 292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 582 2563 2614 3774
Size: 582 1981 51 1160
Fragments arranged by size:
1981 1160 582 51
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2236 2263 2268 2288 2295 2320 2559 2591
Size: 2236 27 5 20 7 25 239 32
Cuts at: 2591 3774
Size: 1183
Fragments arranged by size:
2236 1183 239 32 27 25 20 7
5
Tth111I GACn'n_nGTC
Cuts at: 0 1114 3774
Size: 1114 2660
XbaI T'CTAG_A
Cuts at: 0 1176 3774
Size: 1176 2598
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 589 3774
Size: 589 3185
XhoI C'TCGA_G
Cuts at: 0 508 3644 3774
Size: 508 3136 130
Fragments arranged by size:
3136 508 130
XmnI GAAnn'nnTTC
Cuts at: 0 496 1392 1618 3746 3774
Size: 496 896 226 2128 28
Fragments arranged by size:
2128 896 496 226 28
Enzymes that do cut:
AarI AccI AcuI AleI AlfI ApaI ApoI AseI
AvaI BanI BanII BbsI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BstAPI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI Hin4I HincII MmeI MscI MslI MspA1I
NaeI NgoMIV NheI NsiI NspI PasI PflMI PfoI
PpuMI PshAI PspOMI PspXI PstI PvuII SacI SalI
SanDI SapI SfcI SmlI StuI StyI TaqII TstI
Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaLI AscI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BglI Bpu10I BsaAI BsaBI
BspEI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
ClaI DrdI EciI EcoRI EcoRV FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI NarI
NcoI NdeI NotI NruI PacI PciI PmeI PmlI
PpiI PsiI PsrI PvuI RsrII SacII SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI XmaI ZraI