Restriction map for
pF1KB9404
(Linear) (Six-base) MAPSORT of: KIBB9404.seq Check: 7834 from: 1 to: 2382
KIBB9404.orf
With 182 enzymes: SgfI *
March 31, 2008 15:57 ..
AccI GT'mk_AC
Cuts at: 0 975 2382
Size: 975 1407
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 1688 2382
Size: 283 1405 694
Fragments arranged by size:
1405 694 283
AflIII A'CryG_T
Cuts at: 0 1878 2382
Size: 1878 504
AhdI GACnn_n'nnGTC
Cuts at: 0 2125 2382
Size: 2125 257
ApoI r'AATT_y
Cuts at: 0 402 2382
Size: 402 1980
AseI AT'TA_AT
Cuts at: 0 800 1532 2347 2382
Size: 800 732 815 35
Fragments arranged by size:
815 800 732 35
AvaI C'yCGr_G
Cuts at: 0 896 2382
Size: 896 1486
BamHI G'GATC_C
Cuts at: 0 1659 1807 2382
Size: 1659 148 575
Fragments arranged by size:
1659 575 148
BanII G_rGCy'C
Cuts at: 0 787 2031 2382
Size: 787 1244 351
Fragments arranged by size:
1244 787 351
BbsI GAAGACnn'nnnn_
Cuts at: 0 1190 1298 2382
Size: 1190 108 1084
Fragments arranged by size:
1190 1084 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 264 298 1753 1787 2382
Size: 264 34 1455 34 595
Fragments arranged by size:
1455 595 264 34 34
BclI T'GATC_A
Cuts at: 0 966 2382
Size: 966 1416
BmrI ACTGGGnnnn_n'
Cuts at: 0 192 407 750 2382
Size: 192 215 343 1632
Fragments arranged by size:
1632 343 215 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2045 2382
Size: 2045 337
Bpu10I CC'TnA_GC
Cuts at: 0 332 442 831 2382
Size: 332 110 389 1551
Fragments arranged by size:
1551 389 332 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1337 1399 2382
Size: 937 400 62 983
Fragments arranged by size:
983 937 400 62
BsaBI GATnn'nnATC
Cuts at: 0 395 1229 2382
Size: 395 834 1153
Fragments arranged by size:
1153 834 395
BsaHI Gr'CG_yC
Cuts at: 0 1752 2382
Size: 1752 630
BsaWI w'CCGG_w
Cuts at: 0 1611 2382
Size: 1611 771
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 678 708 2382
Size: 678 30 1674
Fragments arranged by size:
1674 678 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 875 1197 1618 2382
Size: 875 322 421 764
Fragments arranged by size:
875 764 421 322
BseYI C'CCAG_C
Cuts at: 0 1043 2382
Size: 1043 1339
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1013 2382
Size: 1013 1369
Bsp1286I G_dGCh'C
Cuts at: 0 787 2031 2382
Size: 787 1244 351
Fragments arranged by size:
1244 787 351
BspHI T'CATG_A
Cuts at: 0 1968 2382
Size: 1968 414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 237 2382
Size: 237 2145
BstAPI GCAn_nnn'nTGC
Cuts at: 0 291 2382
Size: 291 2091
BstBI TT'CG_AA
Cuts at: 0 1290 2382
Size: 1290 1092
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1137 2382
Size: 1137 1245
BstYI r'GATC_y
Cuts at: 0 1000 1659 1807 2382
Size: 1000 659 148 575
Fragments arranged by size:
1000 659 575 148
BtsI GCAGTG_nn'
Cuts at: 0 1943 2382
Size: 1943 439
DraI TTT'AAA
Cuts at: 0 1545 1683 2382
Size: 1545 138 699
Fragments arranged by size:
1545 699 138
EarI CTCTTCn'nnn_
Cuts at: 0 770 1595 2202 2382
Size: 770 825 607 180
Fragments arranged by size:
825 770 607 180
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 1688 2045 2382
Size: 283 1405 357 337
Fragments arranged by size:
1405 357 337 283
EcoO109I rG'GnC_Cy
Cuts at: 0 2142 2382
Size: 2142 240
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1746 2382
Size: 1746 636
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 157 189 279 311 625 657 1141 1173
Size: 157 32 90 32 314 32 484 32
Cuts at: 1173 1799 1831 2111 2143 2382
Size: 626 32 280 32 239
Fragments arranged by size:
626 484 314 280 239 157 90 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 438 2382
Size: 438 1944
HindIII A'AGCT_T
Cuts at: 0 1669 2382
Size: 1669 713
MfeI C'AATT_G
Cuts at: 0 1427 2258 2382
Size: 1427 831 124
Fragments arranged by size:
1427 831 124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 1186 2220 2382
Size: 871 315 1034 162
Fragments arranged by size:
1034 871 315 162
MslI CAynn'nnrTG
Cuts at: 0 124 962 2084 2382
Size: 124 838 1122 298
Fragments arranged by size:
1122 838 298 124
NspI r_CATG'y
Cuts at: 0 961 1882 2382
Size: 961 921 500
Fragments arranged by size:
961 921 500
PciI A'CATG_T
Cuts at: 0 1878 2382
Size: 1878 504
PflMI CCAn_nnn'nTGG
Cuts at: 0 778 2382
Size: 778 1604
PfoI T'CCnGG_A
Cuts at: 0 33 2382
Size: 33 2349
PpuMI rG'GwC_Cy
Cuts at: 0 2142 2382
Size: 2142 240
PsiI TTA'TAA
Cuts at: 0 419 2382
Size: 419 1963
SapI GCTCTTCn'nnn_
Cuts at: 0 770 2382
Size: 770 1612
SfcI C'TryA_G
Cuts at: 0 987 2382
Size: 987 1395
SmlI C'TyrA_G
Cuts at: 0 952 1352 1414 2382
Size: 952 400 62 968
Fragments arranged by size:
968 952 400 62
SspI AAT'ATT
Cuts at: 0 982 2382
Size: 982 1400
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 995 2382
Size: 995 1387
TatI w'GTAC_w
Cuts at: 0 1558 2382
Size: 1558 824
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 931 963 2382
Size: 931 32 1419
Fragments arranged by size:
1419 931 32
XmnI GAAnn'nnTTC
Cuts at: 0 2009 2348 2382
Size: 2009 339 34
Fragments arranged by size:
2009 339 34
Enzymes that do cut:
AccI AcuI AflIII AhdI ApoI AseI AvaI BamHI
BanII BbsI BcgI BclI BmrI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI Bsp1286I
BspHI BspMI BstAPI BstBI BstXI BstYI BtsI DraI
EarI Eco57MI EcoO109I FalI Hin4I HincII HindIII MfeI
MmeI MslI NspI PciI PflMI PfoI PpuMI PsiI
SapI SfcI SmlI SspI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII
BaeI BanI BbeI BbvCI BciVI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraIII DrdI EaeI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HpaI
KasI KpnI MluI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI StyI SwaI Tth111I XbaI
XcmI XhoI XmaI ZraI