Restriction map for
pF1KB9400
(Linear) (Six-base) MAPSORT of: KIBB9400.seq Check: 4384 from: 1 to: 2523
KIBB9400.orf
With 182 enzymes: SgfI *
December 2, 2013 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 144 2523
Size: 144 2379
AccI GT'mk_AC
Cuts at: 0 1247 2345 2523
Size: 1247 1098 178
Fragments arranged by size:
1247 1098 178
Acc65I G'GTAC_C
Cuts at: 0 296 1995 2523
Size: 296 1699 528
Fragments arranged by size:
1699 528 296
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 2274 2523
Size: 1021 1253 249
Fragments arranged by size:
1253 1021 249
AfeI AGC'GCT
Cuts at: 0 2131 2523
Size: 2131 392
AleI CACnn'nnGTG
Cuts at: 0 808 931 1669 1783 2523
Size: 808 123 738 114 740
Fragments arranged by size:
808 740 738 123 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2377 2523
Size: 2377 146
AlwNI CAG_nnn'CTG
Cuts at: 0 681 1209 1499 2523
Size: 681 528 290 1024
Fragments arranged by size:
1024 681 528 290
ApaI G_GGCC'C
Cuts at: 0 912 2523
Size: 912 1611
ApaLI G'TGCA_C
Cuts at: 0 2157 2523
Size: 2157 366
AvaI C'yCGr_G
Cuts at: 0 169 937 1025 2523
Size: 169 768 88 1498
Fragments arranged by size:
1498 768 169 88
AvrII C'CTAG_G
Cuts at: 0 1045 2523
Size: 1045 1478
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1979 2012 2523
Size: 1979 33 511
Fragments arranged by size:
1979 511 33
BamHI G'GATC_C
Cuts at: 0 941 2523
Size: 941 1582
BanI G'GyrC_C
Cuts at: 0 296 489 933 1473 1511 1632 1769 1995
Size: 296 193 444 540 38 121 137 226
Cuts at: 1995 2523
Size: 528
Fragments arranged by size:
540 528 444 296 226 193 137 121
38
BanII G_rGCy'C
Cuts at: 0 386 481 912 1066 1831 2523
Size: 386 95 431 154 765 692
Fragments arranged by size:
765 692 431 386 154 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 74 2523
Size: 74 2449
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2391 2425 2523
Size: 2391 34 98
Fragments arranged by size:
2391 98 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 554 2523
Size: 554 1969
BclI T'GATC_A
Cuts at: 0 2051 2523
Size: 2051 472
BglI GCCn_nnn'nGGC
Cuts at: 0 26 1061 1859 2523
Size: 26 1035 798 664
Fragments arranged by size:
1035 798 664 26
BlpI GC'TnA_GC
Cuts at: 0 475 526 2058 2389 2523
Size: 475 51 1532 331 134
Fragments arranged by size:
1532 475 331 134 51
Bme1580I G_kGCm'C
Cuts at: 0 494 912 938 1514 1562 1635 2161 2523
Size: 494 418 26 576 48 73 526 362
Fragments arranged by size:
576 526 494 418 362 73 48 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 938 2523
Size: 938 1585
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 130 415 1351 1713 2187 2523
Size: 130 285 936 362 474 336
Fragments arranged by size:
936 474 362 336 285 130
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 900 1244 1400 2523
Size: 703 197 344 156 1123
Fragments arranged by size:
1123 703 344 197 156
Bpu10I CC'TnA_GC
Cuts at: 0 2398 2523
Size: 2398 125
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2173 2523
Size: 2173 350
BsaI GGTCTCn'nnnn_
Cuts at: 0 386 583 1418 1432 2158 2523
Size: 386 197 835 14 726 365
Fragments arranged by size:
835 726 386 365 197 14
BsaAI yAC'GTr
Cuts at: 0 2439 2523
Size: 2439 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1048 1078 1398 1428 2523
Size: 1048 30 320 30 1095
Fragments arranged by size:
1095 1048 320 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 2200 2523
Size: 265 1935 323
Fragments arranged by size:
1935 323 265
BseYI C'CCAG_C
Cuts at: 0 53 963 1570 1824 2066 2383 2523
Size: 53 910 607 254 242 317 140
Fragments arranged by size:
910 607 317 254 242 140 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 1092 2523
Size: 694 398 1431
Fragments arranged by size:
1431 694 398
BsiHKAI G_wGCw'C
Cuts at: 0 356 386 1694 2161 2477 2523
Size: 356 30 1308 467 316 46
Fragments arranged by size:
1308 467 356 316 46 30
BsmI GAATG_Cn'
Cuts at: 0 1102 2523
Size: 1102 1421
BsmBI CGTCTCn'nnnn_
Cuts at: 0 514 2523
Size: 514 2009
Bsp1286I G_dGCh'C
Cuts at: 0 356 386 481 494 912 938 1066 1514
Size: 356 30 95 13 418 26 128 448
Cuts at: 1514 1562 1635 1694 1831 2161 2477 2523
Size: 48 73 59 137 330 316 46
Fragments arranged by size:
448 418 356 330 316 137 128 95
73 59 48 46 30 26 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 144 1660 1831 2369 2523
Size: 144 1516 171 538 154
Fragments arranged by size:
1516 538 171 154 144
BsrDI GCAATG_nn'
Cuts at: 0 713 2523
Size: 713 1810
BsrFI r'CCGG_y
Cuts at: 0 827 2523
Size: 827 1696
BssSI C'ACGA_G
Cuts at: 0 2502 2523
Size: 2502 21
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1097 2523
Size: 1097 1426
BstEII G'GTnAC_C
Cuts at: 0 174 2523
Size: 174 2349
BstXI CCAn_nnnn'nTGG
Cuts at: 0 697 810 952 1615 2035 2175 2379 2523
Size: 697 113 142 663 420 140 204 144
Fragments arranged by size:
697 663 420 204 144 142 140 113
BstYI r'GATC_y
Cuts at: 0 629 707 941 1271 1329 2523
Size: 629 78 234 330 58 1194
Fragments arranged by size:
1194 629 330 234 78 58
Bsu36I CC'TnA_GG
Cuts at: 0 1637 2523
Size: 1637 886
BtgI C'CryG_G
Cuts at: 0 266 593 857 1191 2028 2333 2523
Size: 266 327 264 334 837 305 190
Fragments arranged by size:
837 334 327 305 266 264 190
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 770 2523
Size: 770 1753
BtsI GCAGTG_nn'
Cuts at: 0 431 654 775 1539 2107 2523
Size: 431 223 121 764 568 416
Fragments arranged by size:
764 568 431 416 223 121
DraIII CAC_nnn'GTG
Cuts at: 0 1281 2523
Size: 1281 1242
EaeI y'GGCC_r
Cuts at: 0 509 836 971 2336 2369 2523
Size: 509 327 135 1365 33 154
Fragments arranged by size:
1365 509 327 154 135 33
EarI CTCTTCn'nnn_
Cuts at: 0 229 976 1619 2305 2523
Size: 229 747 643 686 218
Fragments arranged by size:
747 686 643 229 218
EcoICRI GAG'CTC
Cuts at: 0 384 2523
Size: 384 2139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 900 1021 1244 1400 2274 2523
Size: 703 197 121 223 156 874 249
Fragments arranged by size:
874 703 249 223 197 156 121
EcoNI CCTnn'n_nnAGG
Cuts at: 0 226 790 2245 2523
Size: 226 564 1455 278
Fragments arranged by size:
1455 564 278 226
EcoO109I rG'GnC_Cy
Cuts at: 0 909 1041 1574 1905 2523
Size: 909 132 533 331 618
Fragments arranged by size:
909 618 533 331 132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 412 1401 2523
Size: 412 989 1122
Fragments arranged by size:
1122 989 412
FspI TGC'GCA
Cuts at: 0 559 2523
Size: 559 1964
HaeII r_GCGC'y
Cuts at: 0 2133 2511 2523
Size: 2133 378 12
Fragments arranged by size:
2133 378 12
HindIII A'AGCT_T
Cuts at: 0 1103 2523
Size: 1103 1420
KpnI G_GTAC'C
Cuts at: 0 300 1999 2523
Size: 300 1699 524
Fragments arranged by size:
1699 524 300
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1986 2523
Size: 1986 537
MscI TGG'CCA
Cuts at: 0 838 973 2523
Size: 838 135 1550
Fragments arranged by size:
1550 838 135
MslI CAynn'nnrTG
Cuts at: 0 808 931 955 1669 1783 2523
Size: 808 123 24 714 114 740
Fragments arranged by size:
808 740 714 123 114 24
MspA1I CmG'CkG
Cuts at: 0 303 2062 2070 2405 2523
Size: 303 1759 8 335 118
Fragments arranged by size:
1759 335 303 118 8
NaeI GCC'GGC
Cuts at: 0 829 2523
Size: 829 1694
NcoI C'CATG_G
Cuts at: 0 593 1191 2523
Size: 593 598 1332
Fragments arranged by size:
1332 598 593
NgoMIV G'CCGG_C
Cuts at: 0 827 2523
Size: 827 1696
PasI CC'CwG_GG
Cuts at: 0 291 2175 2523
Size: 291 1884 348
Fragments arranged by size:
1884 348 291
PflMI CCAn_nnn'nTGG
Cuts at: 0 1209 1521 2523
Size: 1209 312 1002
Fragments arranged by size:
1209 1002 312
PfoI T'CCnGG_A
Cuts at: 0 2314 2523
Size: 2314 209
PpuMI rG'GwC_Cy
Cuts at: 0 1574 2523
Size: 1574 949
PspOMI G'GGCC_C
Cuts at: 0 908 2523
Size: 908 1615
PstI C_TGCA'G
Cuts at: 0 35 155 611 1093 2236 2523
Size: 35 120 456 482 1143 287
Fragments arranged by size:
1143 482 456 287 120 35
PvuII CAG'CTG
Cuts at: 0 303 2070 2523
Size: 303 1767 453
Fragments arranged by size:
1767 453 303
SacI G_AGCT'C
Cuts at: 0 386 2523
Size: 386 2137
SapI GCTCTTCn'nnn_
Cuts at: 0 2305 2523
Size: 2305 218
SexAI A'CCwGG_T
Cuts at: 0 581 1469 2523
Size: 581 888 1054
Fragments arranged by size:
1054 888 581
SfcI C'TryA_G
Cuts at: 0 31 151 607 1089 1599 2232 2523
Size: 31 120 456 482 510 633 291
Fragments arranged by size:
633 510 482 456 291 120 31
SmaI CCC'GGG
Cuts at: 0 939 1027 2523
Size: 939 88 1496
Fragments arranged by size:
1496 939 88
SmlI C'TyrA_G
Cuts at: 0 2152 2523
Size: 2152 371
SrfI GCCC'GGGC
Cuts at: 0 1027 2523
Size: 1027 1496
StuI AGG'CCT
Cuts at: 0 125 1050 1884 2485 2523
Size: 125 925 834 601 38
Fragments arranged by size:
925 834 601 125 38
StyI C'CwwG_G
Cuts at: 0 387 593 1045 1191 1917 1991 2523
Size: 387 206 452 146 726 74 532
Fragments arranged by size:
726 532 452 387 206 146 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1692 2523
Size: 1692 831
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1048 1080 1398 1430 2523
Size: 1048 32 318 32 1093
Fragments arranged by size:
1093 1048 318 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 288 831 2523
Size: 288 543 1692
Fragments arranged by size:
1692 543 288
XmaI C'CCGG_G
Cuts at: 0 937 1025 2523
Size: 937 88 1498
Fragments arranged by size:
1498 937 88
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AlfI AlwNI
ApaI ApaLI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI HaeII HindIII KpnI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI
PpuMI PspOMI PstI PvuII SacI SapI SexAI SfcI
SmaI SmlI SrfI StuI StyI TaqII TstI XcmI
XmaI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AloI ApoI
AscI AseI AsiSI BbeI BbvCI BfrBI BglII BmgBI
BmtI BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BspHI
BsrBI BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI
DrdI EagI EciI EcoRI EcoRV FseI FspAI Hin4I
HincII HpaI KasI MfeI MluI NarI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI TatI Tth111I XbaI
XhoI XmnI ZraI