Restriction map for
pF1KB9392
(Linear) (Six-base) MAPSORT of: KIBB9392.seq Check: 3801 from: 1 to: 2406
KIBB9392.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1393 2406
Size: 1393 1013
AccI GT'mk_AC
Cuts at: 0 1496 1896 2406
Size: 1496 400 510
Fragments arranged by size:
1496 510 400
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 890 1556 1754 1886 1910 2406
Size: 514 376 666 198 132 24 496
Fragments arranged by size:
666 514 496 376 198 132 24
AfeI AGC'GCT
Cuts at: 0 2275 2406
Size: 2275 131
AflIII A'CryG_T
Cuts at: 0 53 2406
Size: 53 2353
AlwNI CAG_nnn'CTG
Cuts at: 0 229 1080 2124 2406
Size: 229 851 1044 282
Fragments arranged by size:
1044 851 282 229
ApaLI G'TGCA_C
Cuts at: 0 39 1565 2406
Size: 39 1526 841
Fragments arranged by size:
1526 841 39
ApoI r'AATT_y
Cuts at: 0 851 1430 2163 2406
Size: 851 579 733 243
Fragments arranged by size:
851 733 579 243
BanII G_rGCy'C
Cuts at: 0 939 2406
Size: 939 1467
BbsI GAAGACnn'nnnn_
Cuts at: 0 620 820 2406
Size: 620 200 1586
Fragments arranged by size:
1586 620 200
BciVI GTATCCnnnnn_n'
Cuts at: 0 1919 2406
Size: 1919 487
BclI T'GATC_A
Cuts at: 0 231 1578 2406
Size: 231 1347 828
Fragments arranged by size:
1347 828 231
Bme1580I G_kGCm'C
Cuts at: 0 43 351 1569 2406
Size: 43 308 1218 837
Fragments arranged by size:
1218 837 308 43
BmgBI CAC'GTC
Cuts at: 0 38 54 111 2406
Size: 38 16 57 2295
Fragments arranged by size:
2295 57 38 16
BmrI ACTGGGnnnn_n'
Cuts at: 0 1284 1967 2406
Size: 1284 683 439
Fragments arranged by size:
1284 683 439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 953 2406
Size: 953 1453
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 807 1201 2406
Size: 807 394 1205
Fragments arranged by size:
1205 807 394
BsaI GGTCTCn'nnnn_
Cuts at: 0 921 1001 2406
Size: 921 80 1405
Fragments arranged by size:
1405 921 80
BsaAI yAC'GTr
Cuts at: 0 478 2406
Size: 478 1928
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1659 1689 1878 1908 2406
Size: 1659 30 189 30 498
Fragments arranged by size:
1659 498 189 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 2069 2406
Size: 91 1978 337
Fragments arranged by size:
1978 337 91
BsiEI CG_ry'CG
Cuts at: 0 1500 2406
Size: 1500 906
BsiHKAI G_wGCw'C
Cuts at: 0 43 1569 2406
Size: 43 1526 837
Fragments arranged by size:
1526 837 43
BsiWI C'GTAC_G
Cuts at: 0 1303 2406
Size: 1303 1103
BsmI GAATG_Cn'
Cuts at: 0 127 2406
Size: 127 2279
Bsp1286I G_dGCh'C
Cuts at: 0 43 351 939 1569 2406
Size: 43 308 588 630 837
Fragments arranged by size:
837 630 588 308 43
BspHI T'CATG_A
Cuts at: 0 2339 2406
Size: 2339 67
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1393 1857 1906 2406
Size: 1393 464 49 500
Fragments arranged by size:
1393 500 464 49
BsrBI CCG'CTC
Cuts at: 0 90 2406
Size: 90 2316
BsrFI r'CCGG_y
Cuts at: 0 2312 2406
Size: 2312 94
BsrGI T'GTAC_A
Cuts at: 0 2030 2406
Size: 2030 376
BssSI C'ACGA_G
Cuts at: 0 344 692 2406
Size: 344 348 1714
Fragments arranged by size:
1714 348 344
BstEII G'GTnAC_C
Cuts at: 0 1918 2406
Size: 1918 488
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1426 2406
Size: 1426 980
BstYI r'GATC_y
Cuts at: 0 681 2406
Size: 681 1725
BtgI C'CryG_G
Cuts at: 0 1800 2406
Size: 1800 606
DraI TTT'AAA
Cuts at: 0 1704 2406
Size: 1704 702
EaeI y'GGCC_r
Cuts at: 0 1780 2325 2406
Size: 1780 545 81
Fragments arranged by size:
1780 545 81
EarI CTCTTCn'nnn_
Cuts at: 0 139 1142 1263 1827 2406
Size: 139 1003 121 564 579
Fragments arranged by size:
1003 579 564 139 121
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 890 953 1556 1754 1886 1910 2406
Size: 514 376 63 603 198 132 24 496
Fragments arranged by size:
603 514 496 376 198 132 63 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1736 2406
Size: 1736 670
EcoO109I rG'GnC_Cy
Cuts at: 0 316 2406
Size: 316 2090
EcoRI G'AATT_C
Cuts at: 0 1430 2406
Size: 1430 976
HaeII r_GCGC'y
Cuts at: 0 103 132 1821 2277 2406
Size: 103 29 1689 456 129
Fragments arranged by size:
1689 456 129 103 29
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 457 489 1777 1809 1878 1910
Size: 40 32 385 32 1288 32 69 32
Cuts at: 1910 2406
Size: 496
Fragments arranged by size:
1288 496 385 69 40 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1497 2406
Size: 1497 909
MfeI C'AATT_G
Cuts at: 0 2057 2406
Size: 2057 349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 168 1344 2406
Size: 134 34 1176 1062
Fragments arranged by size:
1176 1062 134 34
MscI TGG'CCA
Cuts at: 0 1782 2327 2406
Size: 1782 545 79
Fragments arranged by size:
1782 545 79
MslI CAynn'nnrTG
Cuts at: 0 1424 2406
Size: 1424 982
MspA1I CmG'CkG
Cuts at: 0 210 1576 2406
Size: 210 1366 830
Fragments arranged by size:
1366 830 210
NaeI GCC'GGC
Cuts at: 0 2314 2406
Size: 2314 92
NcoI C'CATG_G
Cuts at: 0 1800 2406
Size: 1800 606
NgoMIV G'CCGG_C
Cuts at: 0 2312 2406
Size: 2312 94
NspI r_CATG'y
Cuts at: 0 1074 2406
Size: 1074 1332
PfoI T'CCnGG_A
Cuts at: 0 768 2406
Size: 768 1638
PmlI CAC'GTG
Cuts at: 0 478 2406
Size: 478 1928
PpuMI rG'GwC_Cy
Cuts at: 0 316 2406
Size: 316 2090
PsiI TTA'TAA
Cuts at: 0 755 1763 2406
Size: 755 1008 643
Fragments arranged by size:
1008 755 643
PstI C_TGCA'G
Cuts at: 0 2363 2406
Size: 2363 43
PvuII CAG'CTG
Cuts at: 0 210 1576 2406
Size: 210 1366 830
Fragments arranged by size:
1366 830 210
SalI G'TCGA_C
Cuts at: 0 1495 2406
Size: 1495 911
ScaI AGT'ACT
Cuts at: 0 1257 2406
Size: 1257 1149
SfcI C'TryA_G
Cuts at: 0 902 2269 2359 2391 2406
Size: 902 1367 90 32 15
Fragments arranged by size:
1367 902 90 32 15
SmlI C'TyrA_G
Cuts at: 0 822 1216 2406
Size: 822 394 1190
Fragments arranged by size:
1190 822 394
StyI C'CwwG_G
Cuts at: 0 417 1800 1855 2406
Size: 417 1383 55 551
Fragments arranged by size:
1383 551 417 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1514 2406
Size: 1514 892
TatI w'GTAC_w
Cuts at: 0 1255 1315 1467 2030 2406
Size: 1255 60 152 563 376
Fragments arranged by size:
1255 563 376 152 60
XbaI T'CTAG_A
Cuts at: 0 663 2406
Size: 663 1743
XmnI GAAnn'nnTTC
Cuts at: 0 853 2406
Size: 853 1553
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AlwNI ApaLI ApoI
BanII BbsI BciVI BclI Bme1580I BmgBI BmrI BpmI
BpuEI BsaI BsaAI BsaXI BseRI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI
BstEII BstXI BstYI BtgI DraI EaeI EarI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I HincII MfeI MmeI
MscI MslI MspA1I NaeI NcoI NgoMIV NspI PfoI
PmlI PpuMI PsiI PstI PvuII SalI ScaI SfcI
SmlI StyI TaqII TatI XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AleI AlfI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BfrBI BglI BglII
BlpI BmtI BplI Bpu10I BsaBI BsaHI BsaWI BseYI
BsgI BsmBI BspEI BsrDI BssHII BstAPI BstBI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraIII DrdI EagI
EciI EcoICRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MluI NarI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PpiI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TstI Tth111I XcmI XhoI XmaI ZraI