Restriction map for
pF1KB9385
(Linear) (Six-base) MAPSORT of: KIBB9385.seq Check: 9154 from: 1 to: 972
KIBB9385.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
Acc65I G'GTAC_C
Cuts at: 0 821 972
Size: 821 151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 834 941 972
Size: 834 107 31
Fragments arranged by size:
834 107 31
AflIII A'CryG_T
Cuts at: 0 107 972
Size: 107 865
AgeI A'CCGG_T
Cuts at: 0 4 972
Size: 4 968
AleI CACnn'nnGTG
Cuts at: 0 112 972
Size: 112 860
AlwNI CAG_nnn'CTG
Cuts at: 0 87 436 972
Size: 87 349 536
Fragments arranged by size:
536 349 87
AvaI C'yCGr_G
Cuts at: 0 37 972
Size: 37 935
BamHI G'GATC_C
Cuts at: 0 369 783 972
Size: 369 414 189
Fragments arranged by size:
414 369 189
BanI G'GyrC_C
Cuts at: 0 169 821 972
Size: 169 652 151
Fragments arranged by size:
652 169 151
BbeI G_GCGC'C
Cuts at: 0 173 972
Size: 173 799
BclI T'GATC_A
Cuts at: 0 258 972
Size: 258 714
BfrBI ATG'CAT
Cuts at: 0 243 972
Size: 243 729
BmgBI CAC'GTC
Cuts at: 0 110 972
Size: 110 862
BmrI ACTGGGnnnn_n'
Cuts at: 0 75 700 972
Size: 75 625 272
Fragments arranged by size:
625 272 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 458 972
Size: 231 227 514
Fragments arranged by size:
514 231 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 972
Size: 136 836
BsaI GGTCTCn'nnnn_
Cuts at: 0 434 972
Size: 434 538
BsaAI yAC'GTr
Cuts at: 0 507 972
Size: 507 465
BsaHI Gr'CG_yC
Cuts at: 0 170 972
Size: 170 802
BsaWI w'CCGG_w
Cuts at: 0 4 972
Size: 4 968
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 42 87 972
Size: 42 45 885
Fragments arranged by size:
885 45 42
BseYI C'CCAG_C
Cuts at: 0 391 972
Size: 391 581
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 972
Size: 529 443
BsmBI CGTCTCn'nnnn_
Cuts at: 0 124 972
Size: 124 848
BsrFI r'CCGG_y
Cuts at: 0 4 132 166 443 972
Size: 4 128 34 277 529
Fragments arranged by size:
529 277 128 34 4
BssSI C'ACGA_G
Cuts at: 0 828 972
Size: 828 144
BstAPI GCAn_nnn'nTGC
Cuts at: 0 844 972
Size: 844 128
BstYI r'GATC_y
Cuts at: 0 369 783 972
Size: 369 414 189
Fragments arranged by size:
414 369 189
Bsu36I CC'TnA_GG
Cuts at: 0 635 972
Size: 635 337
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 819 972
Size: 819 153
EaeI y'GGCC_r
Cuts at: 0 134 972
Size: 134 838
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 231 458 834 941 972
Size: 231 227 376 107 31
Fragments arranged by size:
376 231 227 107 31
EcoO109I rG'GnC_Cy
Cuts at: 0 278 395 972
Size: 278 117 577
Fragments arranged by size:
577 278 117
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 286 675 972
Size: 286 389 297
Fragments arranged by size:
389 297 286
HaeII r_GCGC'y
Cuts at: 0 173 972
Size: 173 799
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 387 419 972
Size: 34 32 321 32 553
Fragments arranged by size:
553 321 34 32 32
KasI G'GCGC_C
Cuts at: 0 169 972
Size: 169 803
KpnI G_GTAC'C
Cuts at: 0 825 972
Size: 825 147
MfeI C'AATT_G
Cuts at: 0 232 972
Size: 232 740
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 972
Size: 223 749
MslI CAynn'nnrTG
Cuts at: 0 112 972
Size: 112 860
NaeI GCC'GGC
Cuts at: 0 134 972
Size: 134 838
NarI GG'CG_CC
Cuts at: 0 170 972
Size: 170 802
NgoMIV G'CCGG_C
Cuts at: 0 132 972
Size: 132 840
NsiI A_TGCA'T
Cuts at: 0 245 972
Size: 245 727
NspI r_CATG'y
Cuts at: 0 208 972
Size: 208 764
PfoI T'CCnGG_A
Cuts at: 0 176 972
Size: 176 796
PpuMI rG'GwC_Cy
Cuts at: 0 278 395 972
Size: 278 117 577
Fragments arranged by size:
577 278 117
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 487 519 972
Size: 487 32 453
Fragments arranged by size:
487 453 32
SanDI GG'GwC_CC
Cuts at: 0 278 972
Size: 278 694
SfoI GGC'GCC
Cuts at: 0 171 972
Size: 171 801
SgrAI Cr'CCGG_yG
Cuts at: 0 166 972
Size: 166 806
SmlI C'TyrA_G
Cuts at: 0 151 972
Size: 151 821
SphI G_CATG'C
Cuts at: 0 208 972
Size: 208 764
SspI AAT'ATT
Cuts at: 0 690 777 972
Size: 690 87 195
Fragments arranged by size:
690 195 87
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 900 932 972
Size: 900 32 40
Fragments arranged by size:
900 40 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 206 972
Size: 206 766
Enzymes that do cut:
Acc65I AcuI AflIII AgeI AleI AlwNI AvaI BamHI
BanI BbeI BclI BfrBI BmgBI BmrI BpmI BpuEI
BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsmBI
BsrFI BssSI BstAPI BstYI Bsu36I BtgZI EaeI Eco57MI
EcoO109I FalI HaeII Hin4I KasI KpnI MfeI MmeI
MslI NaeI NarI NgoMIV NsiI NspI PfoI PpuMI
PsrI SanDI SfoI SgrAI SmlI SphI SspI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AhdI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BanII BbsI BbvCI BcgI BciVI BglI BglII
BlpI Bme1580I BmtI BplI Bpu10I BsaBI BsaXI BsiEI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I BtgI
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI MluI MscI MspA1I NcoI NdeI
NheI NotI NruI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SgfI SmaI SnaBI SpeI SrfI
StuI StyI SwaI TaqII TatI Tth111I XbaI XhoI
XmaI XmnI ZraI