Restriction map for
pF1KB9376
(Linear) (Six-base) MAPSORT of: KIBB9376.seq Check: 3079 from: 1 to: 1233
KIBB9376.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 731 907 1115 1233
Size: 407 324 176 208 118
Fragments arranged by size:
407 324 208 176 118
AleI CACnn'nnGTG
Cuts at: 0 610 1233
Size: 610 623
AlwNI CAG_nnn'CTG
Cuts at: 0 658 959 1018 1233
Size: 658 301 59 215
Fragments arranged by size:
658 301 215 59
ApoI r'AATT_y
Cuts at: 0 144 300 398 431 1233
Size: 144 156 98 33 802
Fragments arranged by size:
802 156 144 98 33
BanI G'GyrC_C
Cuts at: 0 591 1233
Size: 591 642
BanII G_rGCy'C
Cuts at: 0 34 41 1233
Size: 34 7 1192
Fragments arranged by size:
1192 34 7
BclI T'GATC_A
Cuts at: 0 243 1233
Size: 243 990
BfrBI ATG'CAT
Cuts at: 0 82 1233
Size: 82 1151
BmrI ACTGGGnnnn_n'
Cuts at: 0 513 969 1233
Size: 513 456 264
Fragments arranged by size:
513 456 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1040 1233
Size: 1040 193
Bpu10I CC'TnA_GC
Cuts at: 0 33 1233
Size: 33 1200
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 621 651 1233
Size: 621 30 582
Fragments arranged by size:
621 582 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 1233
Size: 289 944
BsiHKAI G_wGCw'C
Cuts at: 0 550 1233
Size: 550 683
BsmBI CGTCTCn'nnnn_
Cuts at: 0 345 1233
Size: 345 888
Bsp1286I G_dGCh'C
Cuts at: 0 34 41 550 1233
Size: 34 7 509 683
Fragments arranged by size:
683 509 34 7
BsrDI GCAATG_nn'
Cuts at: 0 690 935 1233
Size: 690 245 298
Fragments arranged by size:
690 298 245
BsrGI T'GTAC_A
Cuts at: 0 516 1233
Size: 516 717
BtgI C'CryG_G
Cuts at: 0 204 707 1233
Size: 204 503 526
Fragments arranged by size:
526 503 204
BtsI GCAGTG_nn'
Cuts at: 0 272 803 1148 1233
Size: 272 531 345 85
Fragments arranged by size:
531 345 272 85
EaeI y'GGCC_r
Cuts at: 0 422 1233
Size: 422 811
EarI CTCTTCn'nnn_
Cuts at: 0 22 1233
Size: 22 1211
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1 1233
Size: 1 1232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 731 907 1040 1115 1233
Size: 407 324 176 133 75 118
Fragments arranged by size:
407 324 176 133 118 75
EcoRI G'AATT_C
Cuts at: 0 398 431 1233
Size: 398 33 802
Fragments arranged by size:
802 398 33
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 418 1233
Size: 418 815
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 325 357 1233
Size: 325 32 876
Fragments arranged by size:
876 325 32
HindIII A'AGCT_T
Cuts at: 0 521 1233
Size: 521 712
MfeI C'AATT_G
Cuts at: 0 329 1233
Size: 329 904
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 1233
Size: 519 714
MscI TGG'CCA
Cuts at: 0 424 1233
Size: 424 809
MslI CAynn'nnrTG
Cuts at: 0 610 904 1233
Size: 610 294 329
Fragments arranged by size:
610 329 294
MspA1I CmG'CkG
Cuts at: 0 720 791 1093 1233
Size: 720 71 302 140
Fragments arranged by size:
720 302 140 71
NcoI C'CATG_G
Cuts at: 0 204 707 1233
Size: 204 503 526
Fragments arranged by size:
526 503 204
NsiI A_TGCA'T
Cuts at: 0 84 1233
Size: 84 1149
NspI r_CATG'y
Cuts at: 0 909 1233
Size: 909 324
PasI CC'CwG_GG
Cuts at: 0 972 1233
Size: 972 261
PflMI CCAn_nnn'nTGG
Cuts at: 0 658 1233
Size: 658 575
PfoI T'CCnGG_A
Cuts at: 0 289 1233
Size: 289 944
PsiI TTA'TAA
Cuts at: 0 182 1168 1233
Size: 182 986 65
Fragments arranged by size:
986 182 65
PstI C_TGCA'G
Cuts at: 0 719 1233
Size: 719 514
PvuII CAG'CTG
Cuts at: 0 720 791 1093 1233
Size: 720 71 302 140
Fragments arranged by size:
720 302 140 71
SfcI C'TryA_G
Cuts at: 0 715 1088 1233
Size: 715 373 145
Fragments arranged by size:
715 373 145
StyI C'CwwG_G
Cuts at: 0 204 308 707 1154 1233
Size: 204 104 399 447 79
Fragments arranged by size:
447 399 204 104 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 609 1135 1233
Size: 609 526 98
Fragments arranged by size:
609 526 98
TatI w'GTAC_w
Cuts at: 0 516 1233
Size: 516 717
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 360 392 1233
Size: 360 32 841
Fragments arranged by size:
841 360 32
Tth111I GACn'n_nGTC
Cuts at: 0 337 850 1233
Size: 337 513 383
Fragments arranged by size:
513 383 337
XbaI T'CTAG_A
Cuts at: 0 843 1233
Size: 843 390
XmnI GAAnn'nnTTC
Cuts at: 0 666 1083 1233
Size: 666 417 150
Fragments arranged by size:
666 417 150
Enzymes that do cut:
AcuI AleI AlwNI ApoI BanI BanII BclI BfrBI
BmrI BpmI Bpu10I BsaXI BsgI BsiHKAI BsmBI Bsp1286I
BsrDI BsrGI BtgI BtsI EaeI EarI EciI Eco57MI
EcoRI FalI Hin4I HindIII MfeI MmeI MscI MslI
MspA1I NcoI NsiI NspI PasI PflMI PfoI PsiI
PstI PvuII SfcI StyI TaqII TatI TstI Tth111I
XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI
BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PciI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI XcmI XhoI
XmaI ZraI