Restriction map for
pF1KB9290
(Linear) (Six-base) MAPSORT of: KIBB9290.seq Check: 291 from: 1 to: 891
KIBB9290.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AclI AA'CG_TT
Cuts at: 0 479 891
Size: 479 412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 221 697 891
Size: 148 73 476 194
Fragments arranged by size:
476 194 148 73
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 116 148 891
Size: 116 32 743
Fragments arranged by size:
743 116 32
AlwNI CAG_nnn'CTG
Cuts at: 0 54 462 597 891
Size: 54 408 135 294
Fragments arranged by size:
408 294 135 54
ApaLI G'TGCA_C
Cuts at: 0 113 891
Size: 113 778
AvaI C'yCGr_G
Cuts at: 0 440 891
Size: 440 451
BanI G'GyrC_C
Cuts at: 0 790 891
Size: 790 101
BanII G_rGCy'C
Cuts at: 0 769 891
Size: 769 122
BglII A'GATC_T
Cuts at: 0 167 891
Size: 167 724
BlpI GC'TnA_GC
Cuts at: 0 386 891
Size: 386 505
Bme1580I G_kGCm'C
Cuts at: 0 117 561 891
Size: 117 444 330
Fragments arranged by size:
444 330 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 210 560 891
Size: 210 350 331
Fragments arranged by size:
350 331 210
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 184 194 264 544 865 891
Size: 121 63 10 70 280 321 26
Fragments arranged by size:
321 280 121 70 63 26 10
BsaAI yAC'GTr
Cuts at: 0 562 891
Size: 562 329
BsaWI w'CCGG_w
Cuts at: 0 411 891
Size: 411 480
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 43 73 116 146 318 348 409 439
Size: 43 30 43 30 172 30 61 30
Cuts at: 439 891
Size: 452
Fragments arranged by size:
452 172 61 43 43 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 891
Size: 124 767
BseYI C'CCAG_C
Cuts at: 0 597 692 891
Size: 597 95 199
Fragments arranged by size:
597 199 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 891
Size: 163 728
BsiEI CG_ry'CG
Cuts at: 0 729 891
Size: 729 162
BsiHKAI G_wGCw'C
Cuts at: 0 117 227 387 803 851 891
Size: 117 110 160 416 48 40
Fragments arranged by size:
416 160 117 110 48 40
Bsp1286I G_dGCh'C
Cuts at: 0 117 227 387 561 769 803 851 891
Size: 117 110 160 174 208 34 48 40
Fragments arranged by size:
208 174 160 117 110 48 40 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 596 891
Size: 596 295
BsrBI CCG'CTC
Cuts at: 0 731 891
Size: 731 160
BsrFI r'CCGG_y
Cuts at: 0 668 724 891
Size: 668 56 167
Fragments arranged by size:
668 167 56
BstXI CCAn_nnnn'nTGG
Cuts at: 0 843 891
Size: 843 48
BstYI r'GATC_y
Cuts at: 0 167 891
Size: 167 724
Bsu36I CC'TnA_GG
Cuts at: 0 858 891
Size: 858 33
BtgI C'CryG_G
Cuts at: 0 704 891
Size: 704 187
BtsI GCAGTG_nn'
Cuts at: 0 115 891
Size: 115 776
EaeI y'GGCC_r
Cuts at: 0 726 891
Size: 726 165
EagI C'GGCC_G
Cuts at: 0 726 891
Size: 726 165
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 148 184 194 221 264 544 697
Size: 121 27 36 10 27 43 280 153
Cuts at: 697 865 891
Size: 168 26
Fragments arranged by size:
280 168 153 121 43 36 27 27
26 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 46 188 357 760 891
Size: 46 142 169 403 131
Fragments arranged by size:
403 169 142 131 46
EcoO109I rG'GnC_Cy
Cuts at: 0 181 205 212 250 840 891
Size: 181 24 7 38 590 51
Fragments arranged by size:
590 181 51 38 24 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 657 891
Size: 657 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 409 441 891
Size: 43 32 334 32 450
Fragments arranged by size:
450 334 43 32 32
HincII GTy'rAC
Cuts at: 0 286 891
Size: 286 605
MfeI C'AATT_G
Cuts at: 0 501 891
Size: 501 390
MslI CAynn'nnrTG
Cuts at: 0 326 433 891
Size: 326 107 458
Fragments arranged by size:
458 326 107
MspA1I CmG'CkG
Cuts at: 0 162 880 891
Size: 162 718 11
Fragments arranged by size:
718 162 11
NcoI C'CATG_G
Cuts at: 0 704 891
Size: 704 187
PflMI CCAn_nnn'nTGG
Cuts at: 0 597 891
Size: 597 294
PmlI CAC'GTG
Cuts at: 0 562 891
Size: 562 329
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 116 148 891
Size: 116 32 743
Fragments arranged by size:
743 116 32
PpuMI rG'GwC_Cy
Cuts at: 0 181 205 212 250 891
Size: 181 24 7 38 641
Fragments arranged by size:
641 181 38 24 7
PvuII CAG'CTG
Cuts at: 0 162 880 891
Size: 162 718 11
Fragments arranged by size:
718 162 11
SexAI A'CCwGG_T
Cuts at: 0 491 891
Size: 491 400
SmaI CCC'GGG
Cuts at: 0 442 891
Size: 442 449
StyI C'CwwG_G
Cuts at: 0 704 761 836 891
Size: 704 57 75 55
Fragments arranged by size:
704 75 57 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 64 100 684 717 891
Size: 64 36 584 33 174
Fragments arranged by size:
584 174 64 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 297 318 329 350 692 724 891
Size: 297 21 11 21 342 32 167
Fragments arranged by size:
342 297 167 32 21 21 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 69 891
Size: 69 822
XmaI C'CCGG_G
Cuts at: 0 440 891
Size: 440 451
Enzymes that do cut:
AclI AcuI AloI AlwNI ApaLI AvaI BanI BanII
BglII BlpI Bme1580I BmrI BpmI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BstXI BstYI Bsu36I BtgI BtsI EaeI EagI
Eco57MI EcoNI EcoO109I FalI Hin4I HincII MfeI MslI
MspA1I NcoI PflMI PmlI PpiI PpuMI PvuII SexAI
SmaI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BmgBI BmtI BplI Bpu10I BpuEI
BsaI BsaBI BsaHI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI
EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmnI ZraI