Restriction map for
pF1KB9288
(Linear) (Six-base) MAPSORT of: KIBB9288.seq Check: 2800 from: 1 to: 3093
KIBB9288.orf
With 182 enzymes: SgfI *
September 18, 2009 17:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2 3093
Size: 2 3091
AclI AA'CG_TT
Cuts at: 0 2514 3093
Size: 2514 579
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 156 182 1184 1245 1314 1380 1968
Size: 52 104 26 1002 61 69 66 588
Cuts at: 1968 2379 3027 3093
Size: 411 648 66
Fragments arranged by size:
1002 648 588 411 104 69 66 66
61 52 26
AfeI AGC'GCT
Cuts at: 0 2599 3093
Size: 2599 494
AflIII A'CryG_T
Cuts at: 0 1197 1379 2548 3093
Size: 1197 182 1169 545
Fragments arranged by size:
1197 1169 545 182
AhdI GACnn_n'nnGTC
Cuts at: 0 1481 3093
Size: 1481 1612
AleI CACnn'nnGTG
Cuts at: 0 1418 1609 2111 3093
Size: 1418 191 502 982
Fragments arranged by size:
1418 982 502 191
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 796 828 2227 2259 3093
Size: 796 32 1399 32 834
Fragments arranged by size:
1399 834 796 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 156 309 919 1466 3093
Size: 156 153 610 547 1627
Fragments arranged by size:
1627 610 547 156 153
ApaI G_GGCC'C
Cuts at: 0 206 439 3093
Size: 206 233 2654
Fragments arranged by size:
2654 233 206
ApoI r'AATT_y
Cuts at: 0 958 1346 1598 1699 1951 2102 3093
Size: 958 388 252 101 252 151 991
Fragments arranged by size:
991 958 388 252 252 151 101
AvaI C'yCGr_G
Cuts at: 0 432 630 645 3002 3093
Size: 432 198 15 2357 91
Fragments arranged by size:
2357 432 198 91 15
BamHI G'GATC_C
Cuts at: 0 2663 3093
Size: 2663 430
BanI G'GyrC_C
Cuts at: 0 641 3093
Size: 641 2452
BanII G_rGCy'C
Cuts at: 0 206 332 439 451 480 1052 1310 2282
Size: 206 126 107 12 29 572 258 972
Cuts at: 2282 2887 3093
Size: 605 206
Fragments arranged by size:
972 605 572 258 206 206 126 107
29 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 38 188 1423 2786 2864 3044 3093
Size: 38 150 1235 1363 78 180 49
Fragments arranged by size:
1363 1235 180 150 78 49 38
BbvCI CC'TCA_GC
Cuts at: 0 250 3093
Size: 250 2843
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 586 620 3093
Size: 586 34 2473
Fragments arranged by size:
2473 586 34
BclI T'GATC_A
Cuts at: 0 1752 3093
Size: 1752 1341
BglI GCCn_nnn'nGGC
Cuts at: 0 2300 3093
Size: 2300 793
BlpI GC'TnA_GC
Cuts at: 0 896 3093
Size: 896 2197
Bme1580I G_kGCm'C
Cuts at: 0 206 439 646 3093
Size: 206 233 207 2447
Fragments arranged by size:
2447 233 207 206
BmgBI CAC'GTC
Cuts at: 0 1380 3093
Size: 1380 1713
BmrI ACTGGGnnnn_n'
Cuts at: 0 36 3093
Size: 36 3057
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 955 3093
Size: 955 2138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 409 444 568 673 811 925 1628
Size: 92 317 35 124 105 138 114 703
Cuts at: 1628 2375 2871 2887 3093
Size: 747 496 16 206
Fragments arranged by size:
747 703 496 317 206 138 124 114
105 92 35 16
Bpu10I CC'TnA_GC
Cuts at: 0 35 250 310 999 1402 1743 3093
Size: 35 215 60 689 403 341 1350
Fragments arranged by size:
1350 689 403 341 215 60 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1508 3093
Size: 1508 1585
BsaI GGTCTCn'nnnn_
Cuts at: 0 644 688 1123 1760 2673 3093
Size: 644 44 435 637 913 420
Fragments arranged by size:
913 644 637 435 420 44
BsaAI yAC'GTr
Cuts at: 0 574 2551 3093
Size: 574 1977 542
Fragments arranged by size:
1977 574 542
BsaBI GATnn'nnATC
Cuts at: 0 1908 3093
Size: 1908 1185
BsaHI Gr'CG_yC
Cuts at: 0 1653 3093
Size: 1653 1440
BsaWI w'CCGG_w
Cuts at: 0 1565 3093
Size: 1565 1528
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 250 280 641 671 3093
Size: 250 30 361 30 2422
Fragments arranged by size:
2422 361 250 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 385 406 1957 2290 2320 3093
Size: 385 21 1551 333 30 773
Fragments arranged by size:
1551 773 385 333 30 21
BseYI C'CCAG_C
Cuts at: 0 586 683 3093
Size: 586 97 2410
Fragments arranged by size:
2410 586 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 364 473 1479 1657 1824 2394 2771
Size: 133 231 109 1006 178 167 570 377
Cuts at: 2771 3093
Size: 322
Fragments arranged by size:
1006 570 377 322 231 178 167 133
109
BsiHKAI G_wGCw'C
Cuts at: 0 332 2022 2887 3093
Size: 332 1690 865 206
Fragments arranged by size:
1690 865 332 206
Bsp1286I G_dGCh'C
Cuts at: 0 206 332 439 451 480 646 1052 1310
Size: 206 126 107 12 29 166 406 258
Cuts at: 1310 2022 2282 2887 3093
Size: 712 260 605 206
Fragments arranged by size:
712 605 406 260 258 206 206 166
126 107 29 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2 846 3093
Size: 2 844 2247
Fragments arranged by size:
2247 844 2
BsrFI r'CCGG_y
Cuts at: 0 221 3093
Size: 221 2872
BsrGI T'GTAC_A
Cuts at: 0 773 3093
Size: 773 2320
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1802 3093
Size: 1802 1291
BstBI TT'CG_AA
Cuts at: 0 3055 3093
Size: 3055 38
BstEII G'GTnAC_C
Cuts at: 0 720 3093
Size: 720 2373
BstXI CCAn_nnnn'nTGG
Cuts at: 0 14 3093
Size: 14 3079
BstYI r'GATC_y
Cuts at: 0 278 2663 3093
Size: 278 2385 430
Fragments arranged by size:
2385 430 278
Bsu36I CC'TnA_GG
Cuts at: 0 902 2210 3093
Size: 902 1308 883
Fragments arranged by size:
1308 902 883
BtgI C'CryG_G
Cuts at: 0 871 3093
Size: 871 2222
BtsI GCAGTG_nn'
Cuts at: 0 1467 2059 3093
Size: 1467 592 1034
Fragments arranged by size:
1467 1034 592
DraI TTT'AAA
Cuts at: 0 1539 3093
Size: 1539 1554
DraIII CAC_nnn'GTG
Cuts at: 0 1440 3093
Size: 1440 1653
EaeI y'GGCC_r
Cuts at: 0 147 3093
Size: 147 2946
EarI CTCTTCn'nnn_
Cuts at: 0 83 175 1066 1121 2444 2837 3093
Size: 83 92 891 55 1323 393 256
Fragments arranged by size:
1323 891 393 256 92 83 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 151 3093
Size: 151 2942
EcoICRI GAG'CTC
Cuts at: 0 330 2885 3093
Size: 330 2555 208
Fragments arranged by size:
2555 330 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 92 156 182 409 444 568 673
Size: 52 40 64 26 227 35 124 105
Cuts at: 673 811 925 1184 1245 1314 1380 1628 1968
Size: 138 114 259 61 69 66 248 340
Cuts at: 1968 2375 2379 2871 2887 3027 3093
Size: 407 4 492 16 140 66
Fragments arranged by size:
492 407 340 259 248 227 140 138
124 114 105 69 66 66 64 61
52 40 35 26 16 4
EcoO109I rG'GnC_Cy
Cuts at: 0 202 203 405 429 436 671 876 3011
Size: 202 1 202 24 7 235 205 2135
Cuts at: 3011 3093
Size: 82
Fragments arranged by size:
2135 235 205 202 202 82 24 7
1
EcoRI G'AATT_C
Cuts at: 0 1346 2102 3093
Size: 1346 756 991
Fragments arranged by size:
1346 991 756
HaeII r_GCGC'y
Cuts at: 0 2601 3093
Size: 2601 492
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 250 282 403 435 1467 1499 2394 2421
Size: 250 32 121 32 1032 32 895 27
Cuts at: 2421 2426 2453 3093
Size: 5 27 640
Fragments arranged by size:
1032 895 640 250 121 32 32 32
27 27 5
HincII GTy'rAC
Cuts at: 0 2091 2652 3093
Size: 2091 561 441
Fragments arranged by size:
2091 561 441
HindIII A'AGCT_T
Cuts at: 0 1643 2870 3093
Size: 1643 1227 223
Fragments arranged by size:
1643 1227 223
HpaI GTT'AAC
Cuts at: 0 2652 3093
Size: 2652 441
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 1601 1674 3093
Size: 836 765 73 1419
Fragments arranged by size:
1419 836 765 73
MscI TGG'CCA
Cuts at: 0 149 3093
Size: 149 2944
MslI CAynn'nnrTG
Cuts at: 0 495 1014 1247 1280 1418 1455 1539 1609
Size: 495 519 233 33 138 37 84 70
Cuts at: 1609 2043 2111 2370 2465 3093
Size: 434 68 259 95 628
Fragments arranged by size:
628 519 495 434 259 233 138 95
84 70 68 37 33
MspA1I CmG'CkG
Cuts at: 0 2026 3093
Size: 2026 1067
NaeI GCC'GGC
Cuts at: 0 223 3093
Size: 223 2870
NcoI C'CATG_G
Cuts at: 0 871 3093
Size: 871 2222
NdeI CA'TA_TG
Cuts at: 0 1830 1928 2944 3071 3093
Size: 1830 98 1016 127 22
Fragments arranged by size:
1830 1016 127 98 22
NgoMIV G'CCGG_C
Cuts at: 0 221 3093
Size: 221 2872
NspI r_CATG'y
Cuts at: 0 22 888 1201 2194 3093
Size: 22 866 313 993 899
Fragments arranged by size:
993 899 866 313 22
PasI CC'CwG_GG
Cuts at: 0 2013 3093
Size: 2013 1080
PciI A'CATG_T
Cuts at: 0 1197 3093
Size: 1197 1896
PfoI T'CCnGG_A
Cuts at: 0 227 421 689 2903 3093
Size: 227 194 268 2214 190
Fragments arranged by size:
2214 268 227 194 190
PmlI CAC'GTG
Cuts at: 0 574 3093
Size: 574 2519
PpuMI rG'GwC_Cy
Cuts at: 0 405 429 671 3093
Size: 405 24 242 2422
Fragments arranged by size:
2422 405 242 24
PshAI GACnn'nnGTC
Cuts at: 0 1656 2003 3093
Size: 1656 347 1090
Fragments arranged by size:
1656 1090 347
PsiI TTA'TAA
Cuts at: 0 1622 3093
Size: 1622 1471
PspOMI G'GGCC_C
Cuts at: 0 202 435 3093
Size: 202 233 2658
Fragments arranged by size:
2658 233 202
PstI C_TGCA'G
Cuts at: 0 401 1798 2005 3093
Size: 401 1397 207 1088
Fragments arranged by size:
1397 1088 401 207
SacI G_AGCT'C
Cuts at: 0 332 2887 3093
Size: 332 2555 206
Fragments arranged by size:
2555 332 206
SanDI GG'GwC_CC
Cuts at: 0 429 3093
Size: 429 2664
SapI GCTCTTCn'nnn_
Cuts at: 0 175 1066 3093
Size: 175 891 2027
Fragments arranged by size:
2027 891 175
SexAI A'CCwGG_T
Cuts at: 0 653 667 3093
Size: 653 14 2426
Fragments arranged by size:
2426 653 14
SfcI C'TryA_G
Cuts at: 0 397 926 1737 1794 2001 2157 2672 3093
Size: 397 529 811 57 207 156 515 421
Fragments arranged by size:
811 529 515 421 397 207 156 57
SmaI CCC'GGG
Cuts at: 0 434 647 3093
Size: 434 213 2446
Fragments arranged by size:
2446 434 213
SmlI C'TyrA_G
Cuts at: 0 1523 3002 3093
Size: 1523 1479 91
Fragments arranged by size:
1523 1479 91
SphI G_CATG'C
Cuts at: 0 22 2194 3093
Size: 22 2172 899
Fragments arranged by size:
2172 899 22
StuI AGG'CCT
Cuts at: 0 255 2308 3093
Size: 255 2053 785
Fragments arranged by size:
2053 785 255
StyI C'CwwG_G
Cuts at: 0 269 871 3093
Size: 269 602 2222
Fragments arranged by size:
2222 602 269
SwaI ATTT'AAAT
Cuts at: 0 1539 3093
Size: 1539 1554
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 330 1873 3093
Size: 330 1543 1220
Fragments arranged by size:
1543 1220 330
TatI w'GTAC_w
Cuts at: 0 773 3093
Size: 773 2320
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 563 595 3093
Size: 563 32 2498
Fragments arranged by size:
2498 563 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 458 3093
Size: 458 2635
XhoI C'TCGA_G
Cuts at: 0 3002 3093
Size: 3002 91
XmaI C'CCGG_G
Cuts at: 0 432 645 3093
Size: 432 213 2448
Fragments arranged by size:
2448 432 213
XmnI GAAnn'nnTTC
Cuts at: 0 1061 1269 1561 1645 1813 2392 2710 3093
Size: 1061 208 292 84 168 579 318 383
Fragments arranged by size:
1061 579 383 318 292 208 168 84
Enzymes that do cut:
AarI AclI AcuI AfeI AflIII AhdI AleI AloI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtsI DraI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HindIII HpaI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NspI PasI PciI PfoI PmlI PpuMI PshAI PsiI
PspOMI PstI SacI SanDI SapI SexAI SfcI SmaI
SmlI SphI StuI StyI SwaI TaqII TatI TstI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AflII AgeI AlfI ApaLI AscI
AseI AsiSI AvrII BaeI BbeI BciVI BfrBI BglII
BmtI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BssHII BssSI BstZ17I BtgZI ClaI CspCI DrdI
EagI EcoNI EcoRV FalI FseI FspI FspAI KasI
KpnI MfeI MluI NarI NheI NotI NruI NsiI
PacI PflMI PmeI PpiI PspXI PsrI PvuI PvuII
RsrII SacII SalI SbfI ScaI SfiI SfoI SgfI
SgrAI SnaBI SpeI SrfI SspI Tth111I XbaI ZraI