Restriction map for
pF1KB9279
(Linear) (Six-base) MAPSORT of: KIBB9279.seq Check: 4740 from: 1 to: 933
KIBB9279.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 933
Size: 152 781
AflIII A'CryG_T
Cuts at: 0 70 933
Size: 70 863
AleI CACnn'nnGTG
Cuts at: 0 19 933
Size: 19 914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 47 933
Size: 47 886
AlwNI CAG_nnn'CTG
Cuts at: 0 568 933
Size: 568 365
BglII A'GATC_T
Cuts at: 0 784 933
Size: 784 149
BmtI G_CTAG'C
Cuts at: 0 694 933
Size: 694 239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 933
Size: 176 757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 933
Size: 338 595
BsaI GGTCTCn'nnnn_
Cuts at: 0 839 933
Size: 839 94
BsaAI yAC'GTr
Cuts at: 0 763 933
Size: 763 170
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 149 179 933
Size: 149 30 754
Fragments arranged by size:
754 149 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 373 933
Size: 373 560
BsrDI GCAATG_nn'
Cuts at: 0 195 383 933
Size: 195 188 550
Fragments arranged by size:
550 195 188
BstXI CCAn_nnnn'nTGG
Cuts at: 0 264 894 933
Size: 264 630 39
Fragments arranged by size:
630 264 39
BstYI r'GATC_y
Cuts at: 0 784 933
Size: 784 149
BtgI C'CryG_G
Cuts at: 0 149 456 933
Size: 149 307 477
Fragments arranged by size:
477 307 149
EaeI y'GGCC_r
Cuts at: 0 896 933
Size: 896 37
EarI CTCTTCn'nnn_
Cuts at: 0 197 644 761 933
Size: 197 447 117 172
Fragments arranged by size:
447 197 172 117
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 176 933
Size: 152 24 757
Fragments arranged by size:
757 152 24
EcoO109I rG'GnC_Cy
Cuts at: 0 226 253 933
Size: 226 27 680
Fragments arranged by size:
680 226 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 228 933
Size: 228 705
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 149 181 637 669 843 875 933
Size: 149 32 456 32 174 32 58
Fragments arranged by size:
456 174 149 58 32 32 32
HincII GTy'rAC
Cuts at: 0 168 933
Size: 168 765
MscI TGG'CCA
Cuts at: 0 898 933
Size: 898 35
MslI CAynn'nnrTG
Cuts at: 0 19 892 933
Size: 19 873 41
Fragments arranged by size:
873 41 19
MspA1I CmG'CkG
Cuts at: 0 141 373 458 933
Size: 141 232 85 475
Fragments arranged by size:
475 232 141 85
NcoI C'CATG_G
Cuts at: 0 149 933
Size: 149 784
NdeI CA'TA_TG
Cuts at: 0 362 549 933
Size: 362 187 384
Fragments arranged by size:
384 362 187
NheI G'CTAG_C
Cuts at: 0 690 933
Size: 690 243
NspI r_CATG'y
Cuts at: 0 74 540 933
Size: 74 466 393
Fragments arranged by size:
466 393 74
PciI A'CATG_T
Cuts at: 0 70 933
Size: 70 863
PfoI T'CCnGG_A
Cuts at: 0 183 933
Size: 183 750
PshAI GACnn'nnGTC
Cuts at: 0 102 933
Size: 102 831
PsiI TTA'TAA
Cuts at: 0 424 933
Size: 424 509
PstI C_TGCA'G
Cuts at: 0 104 933
Size: 104 829
PvuII CAG'CTG
Cuts at: 0 141 373 933
Size: 141 232 560
Fragments arranged by size:
560 232 141
SacII CC_GC'GG
Cuts at: 0 459 933
Size: 459 474
SfcI C'TryA_G
Cuts at: 0 100 725 933
Size: 100 625 208
Fragments arranged by size:
625 208 100
SmlI C'TyrA_G
Cuts at: 0 353 933
Size: 353 580
StyI C'CwwG_G
Cuts at: 0 149 933
Size: 149 784
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 463 933
Size: 463 470
TatI w'GTAC_w
Cuts at: 0 357 933
Size: 357 576
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 906 933
Size: 906 27
XmnI GAAnn'nnTTC
Cuts at: 0 488 933
Size: 488 445
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI BglII BmtI BpmI
BpuEI BsaI BsaAI BsaXI BspMI BsrDI BstXI BstYI
BtgI EaeI EarI Eco57MI EcoO109I FalI Hin4I HincII
MscI MslI MspA1I NcoI NdeI NheI NspI PciI
PfoI PshAI PsiI PstI PvuII SacII SfcI SmlI
StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BplI Bpu10I BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NgoMIV NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PpuMI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XhoI XmaI ZraI