Restriction map for
pF1KB9273
(Linear) (Six-base) MAPSORT of: KIBB9273.seq Check: 2349 from: 1 to: 1128
KIBB9273.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AccI GT'mk_AC
Cuts at: 0 784 1128
Size: 784 344
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 685 1128
Size: 622 63 443
Fragments arranged by size:
622 443 63
AflIII A'CryG_T
Cuts at: 0 863 1128
Size: 863 265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 953 1035 1128
Size: 953 82 93
Fragments arranged by size:
953 93 82
AlwNI CAG_nnn'CTG
Cuts at: 0 708 1128
Size: 708 420
ApaLI G'TGCA_C
Cuts at: 0 928 1128
Size: 928 200
ApoI r'AATT_y
Cuts at: 0 316 681 1128
Size: 316 365 447
Fragments arranged by size:
447 365 316
BanII G_rGCy'C
Cuts at: 0 827 1128
Size: 827 301
BbsI GAAGACnn'nnnn_
Cuts at: 0 749 1128
Size: 749 379
BbvCI CC'TCA_GC
Cuts at: 0 70 913 1128
Size: 70 843 215
Fragments arranged by size:
843 215 70
BglI GCCn_nnn'nGGC
Cuts at: 0 444 1128
Size: 444 684
BglII A'GATC_T
Cuts at: 0 185 1128
Size: 185 943
Bme1580I G_kGCm'C
Cuts at: 0 932 1128
Size: 932 196
BmgBI CAC'GTC
Cuts at: 0 18 864 1128
Size: 18 846 264
Fragments arranged by size:
846 264 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 123 1128
Size: 123 1005
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1079 1128
Size: 475 604 49
Fragments arranged by size:
604 475 49
Bpu10I CC'TnA_GC
Cuts at: 0 70 913 1128
Size: 70 843 215
Fragments arranged by size:
843 215 70
BsaAI yAC'GTr
Cuts at: 0 219 834 1128
Size: 219 615 294
Fragments arranged by size:
615 294 219
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 478 570 958 1128
Size: 478 92 388 170
Fragments arranged by size:
478 388 170 92
BseYI C'CCAG_C
Cuts at: 0 891 1072 1115 1128
Size: 891 181 43 13
Fragments arranged by size:
891 181 43 13
BsiHKAI G_wGCw'C
Cuts at: 0 932 1128
Size: 932 196
Bsp1286I G_dGCh'C
Cuts at: 0 827 932 1128
Size: 827 105 196
Fragments arranged by size:
827 196 105
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 917 1128
Size: 917 211
BsrDI GCAATG_nn'
Cuts at: 0 365 1128
Size: 365 763
BsrGI T'GTAC_A
Cuts at: 0 899 1128
Size: 899 229
BstAPI GCAn_nnn'nTGC
Cuts at: 0 9 1128
Size: 9 1119
BstEII G'GTnAC_C
Cuts at: 0 82 1128
Size: 82 1046
BstYI r'GATC_y
Cuts at: 0 185 1062 1128
Size: 185 877 66
Fragments arranged by size:
877 185 66
BstZ17I GTA'TAC
Cuts at: 0 785 1128
Size: 785 343
Bsu36I CC'TnA_GG
Cuts at: 0 553 1128
Size: 553 575
BtgI C'CryG_G
Cuts at: 0 53 1128
Size: 53 1075
DraI TTT'AAA
Cuts at: 0 510 1128
Size: 510 618
DraIII CAC_nnn'GTG
Cuts at: 0 21 1128
Size: 21 1107
EaeI y'GGCC_r
Cuts at: 0 56 1122 1128
Size: 56 1066 6
Fragments arranged by size:
1066 56 6
EarI CTCTTCn'nnn_
Cuts at: 0 670 1128
Size: 670 458
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 90 988 1128
Size: 90 898 140
Fragments arranged by size:
898 140 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 622 685 1079 1128
Size: 475 147 63 394 49
Fragments arranged by size:
475 394 147 63 49
EcoO109I rG'GnC_Cy
Cuts at: 0 906 1128
Size: 906 222
HaeII r_GCGC'y
Cuts at: 0 53 1128
Size: 53 1075
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 270 302 1128
Size: 270 32 826
Fragments arranged by size:
826 270 32
MscI TGG'CCA
Cuts at: 0 1124 1128
Size: 1124 4
MslI CAynn'nnrTG
Cuts at: 0 658 1128
Size: 658 470
MspA1I CmG'CkG
Cuts at: 0 499 1056 1128
Size: 499 557 72
Fragments arranged by size:
557 499 72
NspI r_CATG'y
Cuts at: 0 663 1128
Size: 663 465
PflMI CCAn_nnn'nTGG
Cuts at: 0 1008 1128
Size: 1008 120
PfoI T'CCnGG_A
Cuts at: 0 965 1128
Size: 965 163
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 1128
Size: 103 32 993
Fragments arranged by size:
993 103 32
PpuMI rG'GwC_Cy
Cuts at: 0 906 1128
Size: 906 222
PstI C_TGCA'G
Cuts at: 0 713 1128
Size: 713 415
PvuII CAG'CTG
Cuts at: 0 499 1128
Size: 499 629
SexAI A'CCwGG_T
Cuts at: 0 86 720 1128
Size: 86 634 408
Fragments arranged by size:
634 408 86
SfcI C'TryA_G
Cuts at: 0 709 1128
Size: 709 419
SnaBI TAC'GTA
Cuts at: 0 219 1128
Size: 219 909
SpeI A'CTAG_T
Cuts at: 0 1014 1128
Size: 1014 114
StuI AGG'CCT
Cuts at: 0 7 1128
Size: 7 1121
StyI C'CwwG_G
Cuts at: 0 788 1128
Size: 788 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 905 1128
Size: 905 223
TatI w'GTAC_w
Cuts at: 0 142 899 1128
Size: 142 757 229
Fragments arranged by size:
757 229 142
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 718 750 1128
Size: 718 32 378
Fragments arranged by size:
718 378 32
Tth111I GACn'n_nGTC
Cuts at: 0 722 850 1128
Size: 722 128 278
Fragments arranged by size:
722 278 128
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 570 1112 1128
Size: 570 542 16
Fragments arranged by size:
570 542 16
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaLI ApoI BanII
BbsI BbvCI BglI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BsaAI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrDI
BsrGI BstAPI BstEII BstYI BstZ17I Bsu36I BtgI DraI
DraIII EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I
MscI MslI MspA1I NspI PflMI PfoI PpiI PpuMI
PstI PvuII SexAI SfcI SnaBI SpeI StuI StyI
TaqII TatI TstI Tth111I XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BcgI BciVI BclI BfrBI
BlpI BmtI BplI BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstBI BstXI BtgZI BtsI
ClaI CspCI DrdI EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SphI SrfI
SspI SwaI XbaI XhoI XmaI XmnI ZraI