Restriction map for
pF1KB9268
(Linear) (Six-base) MAPSORT of: KIBB9268.seq Check: 2900 from: 1 to: 1728
KIBB9268.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 401 769 1728
Size: 232 169 368 959
Fragments arranged by size:
959 368 232 169
AflIII A'CryG_T
Cuts at: 0 81 1728
Size: 81 1647
AhdI GACnn_n'nnGTC
Cuts at: 0 1111 1728
Size: 1111 617
AlwNI CAG_nnn'CTG
Cuts at: 0 927 1728
Size: 927 801
ApoI r'AATT_y
Cuts at: 0 1432 1728
Size: 1432 296
BanI G'GyrC_C
Cuts at: 0 195 1704 1728
Size: 195 1509 24
Fragments arranged by size:
1509 195 24
BanII G_rGCy'C
Cuts at: 0 1664 1728
Size: 1664 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 104 407 1728
Size: 104 303 1321
Fragments arranged by size:
1321 303 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 820 854 1728
Size: 820 34 874
Fragments arranged by size:
874 820 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 563 1728
Size: 563 1165
BclI T'GATC_A
Cuts at: 0 1029 1166 1728
Size: 1029 137 562
Fragments arranged by size:
1029 562 137
Bme1580I G_kGCm'C
Cuts at: 0 200 1202 1728
Size: 200 1002 526
Fragments arranged by size:
1002 526 200
BmrI ACTGGGnnnn_n'
Cuts at: 0 355 454 1728
Size: 355 99 1274
Fragments arranged by size:
1274 355 99
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 420 1728
Size: 420 1308
BsaI GGTCTCn'nnnn_
Cuts at: 0 1687 1728
Size: 1687 41
BsaBI GATnn'nnATC
Cuts at: 0 694 1728
Size: 694 1034
BsaWI w'CCGG_w
Cuts at: 0 192 1728
Size: 192 1536
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 521 524 679 1728
Size: 521 3 155 1049
Fragments arranged by size:
1049 521 155 3
BseYI C'CCAG_C
Cuts at: 0 68 754 1178 1728
Size: 68 686 424 550
Fragments arranged by size:
686 550 424 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1565 1728
Size: 1565 163
BsiHKAI G_wGCw'C
Cuts at: 0 1664 1728
Size: 1664 64
BsmI GAATG_Cn'
Cuts at: 0 141 1021 1651 1728
Size: 141 880 630 77
Fragments arranged by size:
880 630 141 77
BsmBI CGTCTCn'nnnn_
Cuts at: 0 894 1728
Size: 894 834
Bsp1286I G_dGCh'C
Cuts at: 0 200 1202 1664 1728
Size: 200 1002 462 64
Fragments arranged by size:
1002 462 200 64
BspHI T'CATG_A
Cuts at: 0 159 500 1728
Size: 159 341 1228
Fragments arranged by size:
1228 341 159
BsrGI T'GTAC_A
Cuts at: 0 327 1284 1728
Size: 327 957 444
Fragments arranged by size:
957 444 327
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1186 1715 1728
Size: 1186 529 13
Fragments arranged by size:
1186 529 13
BstYI r'GATC_y
Cuts at: 0 19 1728
Size: 19 1709
Bsu36I CC'TnA_GG
Cuts at: 0 593 1728
Size: 593 1135
BtgI C'CryG_G
Cuts at: 0 207 1127 1728
Size: 207 920 601
Fragments arranged by size:
920 601 207
EaeI y'GGCC_r
Cuts at: 0 224 236 491 1728
Size: 224 12 255 1237
Fragments arranged by size:
1237 255 224 12
EarI CTCTTCn'nnn_
Cuts at: 0 236 1018 1728
Size: 236 782 710
Fragments arranged by size:
782 710 236
EcoICRI GAG'CTC
Cuts at: 0 1662 1728
Size: 1662 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 401 769 1728
Size: 232 169 368 959
Fragments arranged by size:
959 368 232 169
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 775 1728
Size: 775 953
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 600 632 658 690 1062 1094 1097 1129
Size: 600 32 26 32 372 32 3 32
Cuts at: 1129 1728
Size: 599
Fragments arranged by size:
600 599 372 32 32 32 32 26
3
HincII GTy'rAC
Cuts at: 0 14 564 1728
Size: 14 550 1164
Fragments arranged by size:
1164 550 14
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 1370 1728
Size: 962 408 358
Fragments arranged by size:
962 408 358
MscI TGG'CCA
Cuts at: 0 493 1728
Size: 493 1235
MslI CAynn'nnrTG
Cuts at: 0 176 542 1728
Size: 176 366 1186
Fragments arranged by size:
1186 366 176
MspA1I CmG'CkG
Cuts at: 0 72 1045 1728
Size: 72 973 683
Fragments arranged by size:
973 683 72
NcoI C'CATG_G
Cuts at: 0 207 1728
Size: 207 1521
NspI r_CATG'y
Cuts at: 0 85 1728
Size: 85 1643
PasI CC'CwG_GG
Cuts at: 0 848 1728
Size: 848 880
PciI A'CATG_T
Cuts at: 0 81 1728
Size: 81 1647
PfoI T'CCnGG_A
Cuts at: 0 878 1728
Size: 878 850
PshAI GACnn'nnGTC
Cuts at: 0 35 1728
Size: 35 1693
PsiI TTA'TAA
Cuts at: 0 1456 1627 1728
Size: 1456 171 101
Fragments arranged by size:
1456 171 101
PstI C_TGCA'G
Cuts at: 0 77 519 1728
Size: 77 442 1209
Fragments arranged by size:
1209 442 77
PvuII CAG'CTG
Cuts at: 0 72 1045 1728
Size: 72 973 683
Fragments arranged by size:
973 683 72
SacI G_AGCT'C
Cuts at: 0 1664 1728
Size: 1664 64
SapI GCTCTTCn'nnn_
Cuts at: 0 236 1728
Size: 236 1492
SfcI C'TryA_G
Cuts at: 0 73 515 1728
Size: 73 442 1213
Fragments arranged by size:
1213 442 73
SspI AAT'ATT
Cuts at: 0 914 1728
Size: 914 814
StuI AGG'CCT
Cuts at: 0 591 1728
Size: 591 1137
StyI C'CwwG_G
Cuts at: 0 207 221 1527 1728
Size: 207 14 1306 201
Fragments arranged by size:
1306 207 201 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1192 1346 1692 1728
Size: 1192 154 346 36
Fragments arranged by size:
1192 346 154 36
TatI w'GTAC_w
Cuts at: 0 327 1284 1728
Size: 327 957 444
Fragments arranged by size:
957 444 327
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1234 1728
Size: 1234 494
XmnI GAAnn'nnTTC
Cuts at: 0 245 1559 1728
Size: 245 1314 169
Fragments arranged by size:
1314 245 169
Enzymes that do cut:
AcuI AflIII AhdI AlwNI ApoI BanI BanII BbsI
BcgI BciVI BclI Bme1580I BmrI BplI BsaI BsaBI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BsrGI BstXI BstYI Bsu36I BtgI EaeI EarI
EcoICRI Eco57MI FalI Hin4I HincII MmeI MscI MslI
MspA1I NcoI NspI PasI PciI PfoI PshAI PsiI
PstI PvuII SacI SapI SfcI SspI StuI StyI
TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BfrBI BglI
BglII BlpI BmgBI BmtI BpmI Bpu10I BpuEI BsaAI
BsaHI BsaXI BsiEI BsiWI BspEI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI
PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SwaI TstI Tth111I XbaI XhoI XmaI ZraI