Restriction map for
pF1KB9213
(Linear) (Six-base) MAPSORT of: KIBB9213.seq Check: 3212 from: 1 to: 2517
KIBB9213.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AclI AA'CG_TT
Cuts at: 0 2122 2517
Size: 2122 395
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 83 752 1873 1882 2401 2517
Size: 4 79 669 1121 9 519 116
Fragments arranged by size:
1121 669 519 116 79 9 4
AlwNI CAG_nnn'CTG
Cuts at: 0 79 577 609 2517
Size: 79 498 32 1908
Fragments arranged by size:
1908 498 79 32
ApaI G_GGCC'C
Cuts at: 0 1141 2517
Size: 1141 1376
ApaLI G'TGCA_C
Cuts at: 0 1565 2517
Size: 1565 952
ApoI r'AATT_y
Cuts at: 0 388 2517
Size: 388 2129
BanI G'GyrC_C
Cuts at: 0 327 982 2517
Size: 327 655 1535
Fragments arranged by size:
1535 655 327
BanII G_rGCy'C
Cuts at: 0 488 1141 1214 2517
Size: 488 653 73 1303
Fragments arranged by size:
1303 653 488 73
BbsI GAAGACnn'nnnn_
Cuts at: 0 824 1644 2517
Size: 824 820 873
Fragments arranged by size:
873 824 820
BbvCI CC'TCA_GC
Cuts at: 0 830 2517
Size: 830 1687
BciVI GTATCCnnnnn_n'
Cuts at: 0 753 2517
Size: 753 1764
BglII A'GATC_T
Cuts at: 0 771 1065 1770 2517
Size: 771 294 705 747
Fragments arranged by size:
771 747 705 294
BlpI GC'TnA_GC
Cuts at: 0 1592 2517
Size: 1592 925
Bme1580I G_kGCm'C
Cuts at: 0 523 1141 1148 1569 2517
Size: 523 618 7 421 948
Fragments arranged by size:
948 618 523 421 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 1142 2517
Size: 1142 1375
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1067 2152 2517
Size: 1067 1085 365
Fragments arranged by size:
1085 1067 365
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1062 2517
Size: 1062 1455
Bpu10I CC'TnA_GC
Cuts at: 0 648 830 2283 2517
Size: 648 182 1453 234
Fragments arranged by size:
1453 648 234 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 1476 2517
Size: 240 1236 1041
Fragments arranged by size:
1236 1041 240
BsaI GGTCTCn'nnnn_
Cuts at: 0 502 1291 2517
Size: 502 789 1226
Fragments arranged by size:
1226 789 502
BsaAI yAC'GTr
Cuts at: 0 1270 2517
Size: 1270 1247
BsaHI Gr'CG_yC
Cuts at: 0 136 2517
Size: 136 2381
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1040 2517
Size: 1040 1477
BseYI C'CCAG_C
Cuts at: 0 1105 2517
Size: 1105 1412
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1247 2057 2086 2517
Size: 1247 810 29 431
Fragments arranged by size:
1247 810 431 29
BsiHKAI G_wGCw'C
Cuts at: 0 488 1569 2500 2517
Size: 488 1081 931 17
Fragments arranged by size:
1081 931 488 17
BsmI GAATG_Cn'
Cuts at: 0 1717 2517
Size: 1717 800
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1657 2517
Size: 1657 860
Bsp1286I G_dGCh'C
Cuts at: 0 488 523 1141 1148 1214 1569 2500 2517
Size: 488 35 618 7 66 355 931 17
Fragments arranged by size:
931 618 488 355 66 35 17 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 38 2517
Size: 38 2479
BsrBI CCG'CTC
Cuts at: 0 310 2517
Size: 310 2207
BsrDI GCAATG_nn'
Cuts at: 0 561 2018 2517
Size: 561 1457 499
Fragments arranged by size:
1457 561 499
BsrFI r'CCGG_y
Cuts at: 0 359 2517
Size: 359 2158
BsrGI T'GTAC_A
Cuts at: 0 741 2517
Size: 741 1776
BssSI C'ACGA_G
Cuts at: 0 1562 1729 2517
Size: 1562 167 788
Fragments arranged by size:
1562 788 167
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1270 2265 2517
Size: 1270 995 252
Fragments arranged by size:
1270 995 252
BstYI r'GATC_y
Cuts at: 0 771 1065 1770 2517
Size: 771 294 705 747
Fragments arranged by size:
771 747 705 294
Bsu36I CC'TnA_GG
Cuts at: 0 1520 2517
Size: 1520 997
BtgI C'CryG_G
Cuts at: 0 1166 2240 2517
Size: 1166 1074 277
Fragments arranged by size:
1166 1074 277
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 248 321 1415 2450 2517
Size: 248 73 1094 1035 67
Fragments arranged by size:
1094 1035 248 73 67
BtsI GCAGTG_nn'
Cuts at: 0 1650 1699 2517
Size: 1650 49 818
Fragments arranged by size:
1650 818 49
DraI TTT'AAA
Cuts at: 0 663 1799 2517
Size: 663 1136 718
Fragments arranged by size:
1136 718 663
DraIII CAC_nnn'GTG
Cuts at: 0 1270 2517
Size: 1270 1247
DrdI GACnn_nn'nnGTC
Cuts at: 0 2437 2517
Size: 2437 80
EaeI y'GGCC_r
Cuts at: 0 281 1169 2517
Size: 281 888 1348
Fragments arranged by size:
1348 888 281
EarI CTCTTCn'nnn_
Cuts at: 0 680 1058 1289 1433 1872 2420 2517
Size: 680 378 231 144 439 548 97
Fragments arranged by size:
680 548 439 378 231 144 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 2517
Size: 19 2498
EcoICRI GAG'CTC
Cuts at: 0 486 2517
Size: 486 2031
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 83 752 1062 1873 1882 2401 2517
Size: 4 79 669 310 811 9 519 116
Fragments arranged by size:
811 669 519 310 116 79 9 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 34 2517
Size: 34 2483
EcoO109I rG'GnC_Cy
Cuts at: 0 27 613 1137 1138 2517
Size: 27 586 524 1 1379
Fragments arranged by size:
1379 586 524 27 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 100 132 705 737 1114 1146
Size: 55 32 13 32 573 32 377 32
Cuts at: 1146 1188 1220 2517
Size: 42 32 1297
Fragments arranged by size:
1297 573 377 55 42 32 32 32
32 32 13
HincII GTy'rAC
Cuts at: 0 134 1578 2517
Size: 134 1444 939
Fragments arranged by size:
1444 939 134
HindIII A'AGCT_T
Cuts at: 0 1855 2517
Size: 1855 662
MfeI C'AATT_G
Cuts at: 0 1756 2517
Size: 1756 761
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 2041 2517
Size: 455 1586 476
Fragments arranged by size:
1586 476 455
MscI TGG'CCA
Cuts at: 0 1171 2517
Size: 1171 1346
MslI CAynn'nnrTG
Cuts at: 0 1165 1220 1841 2517
Size: 1165 55 621 676
Fragments arranged by size:
1165 676 621 55
MspA1I CmG'CkG
Cuts at: 0 1261 1765 1962 2378 2517
Size: 1261 504 197 416 139
Fragments arranged by size:
1261 504 416 197 139
NcoI C'CATG_G
Cuts at: 0 1166 2240 2517
Size: 1166 1074 277
Fragments arranged by size:
1166 1074 277
NdeI CA'TA_TG
Cuts at: 0 375 2517
Size: 375 2142
NspI r_CATG'y
Cuts at: 0 1692 2517
Size: 1692 825
PasI CC'CwG_GG
Cuts at: 0 141 2517
Size: 141 2376
PflMI CCAn_nnn'nTGG
Cuts at: 0 979 2517
Size: 979 1538
PfoI T'CCnGG_A
Cuts at: 0 838 2517
Size: 838 1679
PpuMI rG'GwC_Cy
Cuts at: 0 27 613 2517
Size: 27 586 1904
Fragments arranged by size:
1904 586 27
PspOMI G'GGCC_C
Cuts at: 0 1137 2517
Size: 1137 1380
PstI C_TGCA'G
Cuts at: 0 35 1770 2517
Size: 35 1735 747
Fragments arranged by size:
1735 747 35
PvuII CAG'CTG
Cuts at: 0 1765 1962 2378 2517
Size: 1765 197 416 139
Fragments arranged by size:
1765 416 197 139
SacI G_AGCT'C
Cuts at: 0 488 2517
Size: 488 2029
SapI GCTCTTCn'nnn_
Cuts at: 0 2420 2517
Size: 2420 97
SbfI CC_TGCA'GG
Cuts at: 0 35 2517
Size: 35 2482
SexAI A'CCwGG_T
Cuts at: 0 945 2517
Size: 945 1572
SfcI C'TryA_G
Cuts at: 0 31 66 1766 2517
Size: 31 35 1700 751
Fragments arranged by size:
1700 751 35 31
SmlI C'TyrA_G
Cuts at: 0 219 1455 2517
Size: 219 1236 1062
Fragments arranged by size:
1236 1062 219
StyI C'CwwG_G
Cuts at: 0 1166 2240 2517
Size: 1166 1074 277
Fragments arranged by size:
1166 1074 277
TatI w'GTAC_w
Cuts at: 0 741 889 1503 2517
Size: 741 148 614 1014
Fragments arranged by size:
1014 741 614 148
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 41 73 2517
Size: 41 32 2444
Fragments arranged by size:
2444 41 32
Tth111I GACn'n_nGTC
Cuts at: 0 112 2517
Size: 112 2405
XbaI T'CTAG_A
Cuts at: 0 1068 1206 1974 2517
Size: 1068 138 768 543
Fragments arranged by size:
1068 768 543 138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 798 1182 2517
Size: 798 384 1335
Fragments arranged by size:
1335 798 384
Enzymes that do cut:
AclI AcuI AlwNI ApaI ApaLI ApoI BanI BanII
BbsI BbvCI BciVI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I Hin4I HincII HindIII MfeI MmeI
MscI MslI MspA1I NcoI NdeI NspI PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SapI SbfI
SexAI SfcI SmlI StyI TatI TstI Tth111I XbaI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BclI BfrBI BglI
BmgBI BmtI BsaBI BsaWI BsaXI BsiEI BsiWI BspEI
BspHI BssHII BstBI BstEII BstXI BstZ17I ClaI CspCI
EagI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII XhoI
XmaI XmnI ZraI