Restriction map for
pF1KB9171
(Linear) (Six-base) MAPSORT of: KIBB9171.seq Check: 6797 from: 1 to: 2007
KIBB9171.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 740 858 1410 2007
Size: 740 118 552 597
Fragments arranged by size:
740 597 552 118
AatII G_ACGT'C
Cuts at: 0 1286 1931 2007
Size: 1286 645 76
Fragments arranged by size:
1286 645 76
AgeI A'CCGG_T
Cuts at: 0 1654 2007
Size: 1654 353
AlwNI CAG_nnn'CTG
Cuts at: 0 255 325 864 873 960 1453 1515 1938
Size: 255 70 539 9 87 493 62 423
Cuts at: 1938 2007
Size: 69
Fragments arranged by size:
539 493 423 255 87 70 69 62
9
ApaI G_GGCC'C
Cuts at: 0 1762 2007
Size: 1762 245
ApaLI G'TGCA_C
Cuts at: 0 187 517 2007
Size: 187 330 1490
Fragments arranged by size:
1490 330 187
AvaI C'yCGr_G
Cuts at: 0 61 288 394 1355 1865 2007
Size: 61 227 106 961 510 142
Fragments arranged by size:
961 510 227 142 106 61
AvrII C'CTAG_G
Cuts at: 0 231 1896 2007
Size: 231 1665 111
Fragments arranged by size:
1665 231 111
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 276 309 2007
Size: 276 33 1698
Fragments arranged by size:
1698 276 33
BamHI G'GATC_C
Cuts at: 0 494 533 1856 2007
Size: 494 39 1323 151
Fragments arranged by size:
1323 494 151 39
BanI G'GyrC_C
Cuts at: 0 398 581 999 1884 2007
Size: 398 183 418 885 123
Fragments arranged by size:
885 418 398 183 123
BanII G_rGCy'C
Cuts at: 0 1105 1189 1509 1762 2007
Size: 1105 84 320 253 245
Fragments arranged by size:
1105 320 253 245 84
BbeI G_GCGC'C
Cuts at: 0 1003 2007
Size: 1003 1004
BciVI GTATCCnnnnn_n'
Cuts at: 0 216 2007
Size: 216 1791
BglI GCCn_nnn'nGGC
Cuts at: 0 160 322 396 1757 2007
Size: 160 162 74 1361 250
Fragments arranged by size:
1361 250 162 160 74
Bme1580I G_kGCm'C
Cuts at: 0 191 401 521 1017 1038 1762 2007
Size: 191 210 120 496 21 724 245
Fragments arranged by size:
724 496 245 210 191 120 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 519 2007
Size: 519 1488
BmtI G_CTAG'C
Cuts at: 0 1852 2007
Size: 1852 155
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1851 2007
Size: 1851 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 922 940 1054 1430 1535 1846 1981
Size: 377 545 18 114 376 105 311 135
Cuts at: 1981 2007
Size: 26
Fragments arranged by size:
545 377 376 311 135 114 105 26
18
Bpu10I CC'TnA_GC
Cuts at: 0 83 764 1666 2007
Size: 83 681 902 341
Fragments arranged by size:
902 681 341 83
BsaI GGTCTCn'nnnn_
Cuts at: 0 214 783 2007
Size: 214 569 1224
Fragments arranged by size:
1224 569 214
BsaAI yAC'GTr
Cuts at: 0 714 2007
Size: 714 1293
BsaHI Gr'CG_yC
Cuts at: 0 272 1000 1283 1928 2007
Size: 272 728 283 645 79
Fragments arranged by size:
728 645 283 272 79
BsaWI w'CCGG_w
Cuts at: 0 259 1654 2007
Size: 259 1395 353
Fragments arranged by size:
1395 353 259
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 925 955 1954 1984 2007
Size: 925 30 999 30 23
Fragments arranged by size:
999 925 30 30 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 256 1330 1375 1587 1843 2007
Size: 187 69 1074 45 212 256 164
Fragments arranged by size:
1074 256 212 187 164 69 45
BseYI C'CCAG_C
Cuts at: 0 177 559 963 1105 1734 1844 2007
Size: 177 382 404 142 629 110 163
Fragments arranged by size:
629 404 382 177 163 142 110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 712 1163 1358 1754 2007
Size: 446 266 451 195 396 253
Fragments arranged by size:
451 446 396 266 253 195
BsiEI CG_ry'CG
Cuts at: 0 885 2007
Size: 885 1122
BsiHKAI G_wGCw'C
Cuts at: 0 191 353 521 1189 2007
Size: 191 162 168 668 818
Fragments arranged by size:
818 668 191 168 162
Bsp1286I G_dGCh'C
Cuts at: 0 191 353 401 521 1017 1038 1105 1189
Size: 191 162 48 120 496 21 67 84
Cuts at: 1189 1509 1762 2007
Size: 320 253 245
Fragments arranged by size:
496 320 253 245 191 162 120 84
67 48 21
BspEI T'CCGG_A
Cuts at: 0 259 2007
Size: 259 1748
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 683 740 858 1410 2007
Size: 683 57 118 552 597
Fragments arranged by size:
683 597 552 118 57
BsrDI GCAATG_nn'
Cuts at: 0 1790 2007
Size: 1790 217
BsrFI r'CCGG_y
Cuts at: 0 877 1544 1654 2007
Size: 877 667 110 353
Fragments arranged by size:
877 667 353 110
BssHII G'CGCG_C
Cuts at: 0 305 2007
Size: 305 1702
BssSI C'ACGA_G
Cuts at: 0 1342 2007
Size: 1342 665
BstAPI GCAn_nnn'nTGC
Cuts at: 0 735 2007
Size: 735 1272
BstEII G'GTnAC_C
Cuts at: 0 967 2007
Size: 967 1040
BstYI r'GATC_y
Cuts at: 0 494 533 1856 2007
Size: 494 39 1323 151
Fragments arranged by size:
1323 494 151 39
Bsu36I CC'TnA_GG
Cuts at: 0 812 1309 2007
Size: 812 497 698
Fragments arranged by size:
812 698 497
BtgI C'CryG_G
Cuts at: 0 402 522 1500 1746 2007
Size: 402 120 978 246 261
Fragments arranged by size:
978 402 261 246 120
BtsI GCAGTG_nn'
Cuts at: 0 300 644 1370 1639 1990 2007
Size: 300 344 726 269 351 17
Fragments arranged by size:
726 351 344 300 269 17
DraIII CAC_nnn'GTG
Cuts at: 0 2001 2007
Size: 2001 6
DrdI GACnn_nn'nnGTC
Cuts at: 0 562 2007
Size: 562 1445
EaeI y'GGCC_r
Cuts at: 0 35 314 405 449 525 875 882 1743
Size: 35 279 91 44 76 350 7 861
Cuts at: 1743 2007
Size: 264
Fragments arranged by size:
861 350 279 264 91 76 44 35
7
EagI C'GGCC_G
Cuts at: 0 882 2007
Size: 882 1125
EarI CTCTTCn'nnn_
Cuts at: 0 164 1057 2007
Size: 164 893 950
Fragments arranged by size:
950 893 164
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 946 2007
Size: 946 1061
EcoICRI GAG'CTC
Cuts at: 0 1187 2007
Size: 1187 820
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 922 940 1054 1430 1535 1846 1981
Size: 377 545 18 114 376 105 311 135
Cuts at: 1981 2007
Size: 26
Fragments arranged by size:
545 377 376 311 135 114 105 26
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 235 1312 1332 1825 1895 2007
Size: 235 1077 20 493 70 112
Fragments arranged by size:
1077 493 235 112 70 20
EcoO109I rG'GnC_Cy
Cuts at: 0 228 654 807 1050 1365 1738 1758 1759
Size: 228 426 153 243 315 373 20 1
Cuts at: 1759 1900 1935 2007
Size: 141 35 72
Fragments arranged by size:
426 373 315 243 228 153 141 72
35 20 1
EcoRV GAT'ATC
Cuts at: 0 1260 2007
Size: 1260 747
HaeII r_GCGC'y
Cuts at: 0 90 256 603 1003 1735 2007
Size: 90 166 347 400 732 272
Fragments arranged by size:
732 400 347 272 166 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 63 95 190 222 525 557 641 673
Size: 63 32 95 32 303 32 84 32
Cuts at: 673 2007
Size: 1334
Fragments arranged by size:
1334 303 95 84 63 32 32 32
32
HincII GTy'rAC
Cuts at: 0 672 2007
Size: 672 1335
KasI G'GCGC_C
Cuts at: 0 999 2007
Size: 999 1008
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1314 2007
Size: 1314 693
MscI TGG'CCA
Cuts at: 0 407 451 527 1745 2007
Size: 407 44 76 1218 262
Fragments arranged by size:
1218 407 262 76 44
MslI CAynn'nnrTG
Cuts at: 0 587 2007
Size: 587 1420
MspA1I CmG'CkG
Cuts at: 0 336 460 861 1090 1171 1237 1539 1959
Size: 336 124 401 229 81 66 302 420
Cuts at: 1959 2007
Size: 48
Fragments arranged by size:
420 401 336 302 229 124 81 66
48
NaeI GCC'GGC
Cuts at: 0 879 2007
Size: 879 1128
NarI GG'CG_CC
Cuts at: 0 1000 2007
Size: 1000 1007
NcoI C'CATG_G
Cuts at: 0 402 522 1746 2007
Size: 402 120 1224 261
Fragments arranged by size:
1224 402 261 120
NgoMIV G'CCGG_C
Cuts at: 0 877 2007
Size: 877 1130
NheI G'CTAG_C
Cuts at: 0 1848 2007
Size: 1848 159
NspI r_CATG'y
Cuts at: 0 469 2007
Size: 469 1538
PasI CC'CwG_GG
Cuts at: 0 1530 2007
Size: 1530 477
PfoI T'CCnGG_A
Cuts at: 0 1193 1823 2007
Size: 1193 630 184
Fragments arranged by size:
1193 630 184
PpuMI rG'GwC_Cy
Cuts at: 0 228 807 2007
Size: 228 579 1200
Fragments arranged by size:
1200 579 228
PshAI GACnn'nnGTC
Cuts at: 0 226 2007
Size: 226 1781
PspOMI G'GGCC_C
Cuts at: 0 1758 2007
Size: 1758 249
PstI C_TGCA'G
Cuts at: 0 251 335 869 1461 1757 2007
Size: 251 84 534 592 296 250
Fragments arranged by size:
592 534 296 251 250 84
PvuII CAG'CTG
Cuts at: 0 336 460 861 1171 1539 1959 2007
Size: 336 124 401 310 368 420 48
Fragments arranged by size:
420 401 368 336 310 124 48
SacI G_AGCT'C
Cuts at: 0 1189 2007
Size: 1189 818
SanDI GG'GwC_CC
Cuts at: 0 807 2007
Size: 807 1200
SapI GCTCTTCn'nnn_
Cuts at: 0 1057 2007
Size: 1057 950
SbfI CC_TGCA'GG
Cuts at: 0 1757 2007
Size: 1757 250
SexAI A'CCwGG_T
Cuts at: 0 686 1718 1997 2007
Size: 686 1032 279 10
Fragments arranged by size:
1032 686 279 10
SfcI C'TryA_G
Cuts at: 0 247 331 865 1457 1753 2007
Size: 247 84 534 592 296 254
Fragments arranged by size:
592 534 296 254 247 84
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1757 2007
Size: 1757 250
SfoI GGC'GCC
Cuts at: 0 1001 2007
Size: 1001 1006
SmaI CCC'GGG
Cuts at: 0 396 1357 1867 2007
Size: 396 961 510 140
Fragments arranged by size:
961 510 396 140
StuI AGG'CCT
Cuts at: 0 163 390 1131 1493 2007
Size: 163 227 741 362 514
Fragments arranged by size:
741 514 362 227 163
StyI C'CwwG_G
Cuts at: 0 231 402 522 1746 1896 2007
Size: 231 171 120 1224 150 111
Fragments arranged by size:
1224 231 171 150 120 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 955 2007
Size: 955 1052
TatI w'GTAC_w
Cuts at: 0 951 2007
Size: 951 1056
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 121 153 925 957 1189 1211 1221 1243
Size: 121 32 772 32 232 22 10 22
Cuts at: 1243 1954 1986 2007
Size: 711 32 21
Fragments arranged by size:
772 711 232 121 32 32 32 22
22 21 10
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1473 2007
Size: 1473 534
XmaI C'CCGG_G
Cuts at: 0 394 1355 1865 2007
Size: 394 961 510 142
Fragments arranged by size:
961 510 394 142
ZraI GAC'GTC
Cuts at: 0 1284 1929 2007
Size: 1284 645 78
Fragments arranged by size:
1284 645 78
Enzymes that do cut:
AarI AatII AgeI AlwNI ApaI ApaLI AvaI AvrII
BaeI BamHI BanI BanII BbeI BciVI BglI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrDI BsrFI BssHII BssSI BstAPI BstEII
BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NheI NspI PasI PfoI PpuMI
PshAI PspOMI PstI PvuII SacI SanDI SapI SbfI
SexAI SfcI SfiI SfoI SmaI StuI StyI TaqII
TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI BbsI
BbvCI BcgI BclI BfrBI BglII BlpI BmgBI BpuEI
BsaBI BsiWI BsmI BsmBI BspHI BsrBI BsrGI BstBI
BstXI BstZ17I BtgZI ClaI CspCI DraI EcoRI FalI
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
NdeI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII
SalI ScaI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI