Restriction map for
pF1KB9134
(Linear) (Six-base) MAPSORT of: KIBB9134.seq Check: 5070 from: 1 to: 3936
KIBB9134.orf
With 182 enzymes: SgfI *
July 27, 2015 09:05 ..
AccI GT'mk_AC
Cuts at: 0 968 1056 3936
Size: 968 88 2880
Fragments arranged by size:
2880 968 88
AclI AA'CG_TT
Cuts at: 0 3658 3936
Size: 3658 278
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 658 1394 2360 2608 2708 3586 3936
Size: 66 592 736 966 248 100 878 350
Fragments arranged by size:
966 878 736 592 350 248 100 66
AflIII A'CryG_T
Cuts at: 0 1600 3936
Size: 1600 2336
AhdI GACnn_n'nnGTC
Cuts at: 0 596 3936
Size: 596 3340
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3914 3936
Size: 3914 22
AlwNI CAG_nnn'CTG
Cuts at: 0 2074 2658 3526 3792 3936
Size: 2074 584 868 266 144
Fragments arranged by size:
2074 868 584 266 144
ApoI r'AATT_y
Cuts at: 0 328 1182 3400 3853 3936
Size: 328 854 2218 453 83
Fragments arranged by size:
2218 854 453 328 83
AseI AT'TA_AT
Cuts at: 0 1108 1760 2785 3936
Size: 1108 652 1025 1151
Fragments arranged by size:
1151 1108 1025 652
BanII G_rGCy'C
Cuts at: 0 240 391 1442 3936
Size: 240 151 1051 2494
Fragments arranged by size:
2494 1051 240 151
BbsI GAAGACnn'nnnn_
Cuts at: 0 1736 1883 3936
Size: 1736 147 2053
Fragments arranged by size:
2053 1736 147
BbvCI CC'TCA_GC
Cuts at: 0 3299 3936
Size: 3299 637
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1054 1088 3581 3615 3936
Size: 1054 34 2493 34 321
Fragments arranged by size:
2493 1054 321 34 34
BclI T'GATC_A
Cuts at: 0 419 672 3936
Size: 419 253 3264
Fragments arranged by size:
3264 419 253
BglI GCCn_nnn'nGGC
Cuts at: 0 1975 2205 3936
Size: 1975 230 1731
Fragments arranged by size:
1975 1731 230
BglII A'GATC_T
Cuts at: 0 292 3936
Size: 292 3644
BlpI GC'TnA_GC
Cuts at: 0 2441 3936
Size: 2441 1495
Bme1580I G_kGCm'C
Cuts at: 0 2201 3936
Size: 2201 1735
BmrI ACTGGGnnnn_n'
Cuts at: 0 690 1324 1995 3936
Size: 690 634 671 1941
Fragments arranged by size:
1941 690 671 634
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 2000 2108 2680 3936
Size: 182 1818 108 572 1256
Fragments arranged by size:
1818 1256 572 182 108
Bpu10I CC'TnA_GC
Cuts at: 0 3299 3936
Size: 3299 637
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 2119 3936
Size: 203 1916 1817
Fragments arranged by size:
1916 1817 203
BsaAI yAC'GTr
Cuts at: 0 1603 3442 3936
Size: 1603 1839 494
Fragments arranged by size:
1839 1603 494
BsaBI GATnn'nnATC
Cuts at: 0 1076 3936
Size: 1076 2860
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2677 3936
Size: 2677 1259
BseYI C'CCAG_C
Cuts at: 0 2187 3936
Size: 2187 1749
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 3936
Size: 419 3517
BsiHKAI G_wGCw'C
Cuts at: 0 391 1442 3889 3936
Size: 391 1051 2447 47
Fragments arranged by size:
2447 1051 391 47
BsmI GAATG_Cn'
Cuts at: 0 23 2971 3936
Size: 23 2948 965
Fragments arranged by size:
2948 965 23
Bsp1286I G_dGCh'C
Cuts at: 0 240 391 1442 2201 3889 3936
Size: 240 151 1051 759 1688 47
Fragments arranged by size:
1688 1051 759 240 151 47
BspHI T'CATG_A
Cuts at: 0 3008 3804 3936
Size: 3008 796 132
Fragments arranged by size:
3008 796 132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 570 2721 3135 3936
Size: 570 2151 414 801
Fragments arranged by size:
2151 801 570 414
BsrDI GCAATG_nn'
Cuts at: 0 1491 3680 3936
Size: 1491 2189 256
Fragments arranged by size:
2189 1491 256
BsrGI T'GTAC_A
Cuts at: 0 198 2702 3936
Size: 198 2504 1234
Fragments arranged by size:
2504 1234 198
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1228 3150 3936
Size: 1228 1922 786
Fragments arranged by size:
1922 1228 786
BstYI r'GATC_y
Cuts at: 0 292 1405 3357 3936
Size: 292 1113 1952 579
Fragments arranged by size:
1952 1113 579 292
BtsI GCAGTG_nn'
Cuts at: 0 3609 3912 3936
Size: 3609 303 24
Fragments arranged by size:
3609 303 24
ClaI AT'CG_AT
Cuts at: 0 3878 3936
Size: 3878 58
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3028 3063 3936
Size: 3028 35 873
Fragments arranged by size:
3028 873 35
DraI TTT'AAA
Cuts at: 0 333 3936
Size: 333 3603
EarI CTCTTCn'nnn_
Cuts at: 0 863 1850 3176 3936
Size: 863 987 1326 760
Fragments arranged by size:
1326 987 863 760
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3552 3936
Size: 3552 384
EcoICRI GAG'CTC
Cuts at: 0 389 1440 3936
Size: 389 1051 2496
Fragments arranged by size:
2496 1051 389
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 66 182 658 1394 2000 2108 2360 2608
Size: 66 116 476 736 606 108 252 248
Cuts at: 2608 2680 2708 3586 3936
Size: 72 28 878 350
Fragments arranged by size:
878 736 606 476 350 252 248 116
108 72 66 28
EcoO109I rG'GnC_Cy
Cuts at: 0 1547 3936
Size: 1547 2389
EcoRV GAT'ATC
Cuts at: 0 2630 3936
Size: 2630 1306
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3079 3936
Size: 3079 857
HaeII r_GCGC'y
Cuts at: 0 2289 3936
Size: 2289 1647
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 404 411 436 443 582 614 657 689
Size: 404 7 25 7 139 32 43 32
Cuts at: 689 1321 1353 1849 1881 2089 2121 2568 2596
Size: 632 32 496 32 208 32 447 28
Cuts at: 2596 2600 2628 3871 3903 3936
Size: 4 28 1243 32 33
Fragments arranged by size:
1243 632 496 447 404 208 139 43
33 32 32 32 32 32 32 28
28 25 7 7 4
HincII GTy'rAC
Cuts at: 0 701 2141 2484 3936
Size: 701 1440 343 1452
Fragments arranged by size:
1452 1440 701 343
HindIII A'AGCT_T
Cuts at: 0 656 2551 3936
Size: 656 1895 1385
Fragments arranged by size:
1895 1385 656
HpaI GTT'AAC
Cuts at: 0 701 3936
Size: 701 3235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 1379 2499 3158 3936
Size: 84 1295 1120 659 778
Fragments arranged by size:
1295 1120 778 659 84
MslI CAynn'nnrTG
Cuts at: 0 1679 3936
Size: 1679 2257
MspA1I CmG'CkG
Cuts at: 0 1062 3936
Size: 1062 2874
NspI r_CATG'y
Cuts at: 0 3742 3936
Size: 3742 194
PasI CC'CwG_GG
Cuts at: 0 3918 3936
Size: 3918 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 1572 3792 3936
Size: 1572 2220 144
Fragments arranged by size:
2220 1572 144
PfoI T'CCnGG_A
Cuts at: 0 4 174 3936
Size: 4 170 3762
Fragments arranged by size:
3762 170 4
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 767 799 3936
Size: 767 32 3137
Fragments arranged by size:
3137 767 32
PpuMI rG'GwC_Cy
Cuts at: 0 1547 3936
Size: 1547 2389
PsiI TTA'TAA
Cuts at: 0 3374 3545 3936
Size: 3374 171 391
Fragments arranged by size:
3374 391 171
PstI C_TGCA'G
Cuts at: 0 2264 3936
Size: 2264 1672
PvuII CAG'CTG
Cuts at: 0 1062 3936
Size: 1062 2874
SacI G_AGCT'C
Cuts at: 0 391 1442 3936
Size: 391 1051 2494
Fragments arranged by size:
2494 1051 391
SapI GCTCTTCn'nnn_
Cuts at: 0 1850 3936
Size: 1850 2086
SfcI C'TryA_G
Cuts at: 0 1057 2260 3858 3936
Size: 1057 1203 1598 78
Fragments arranged by size:
1598 1203 1057 78
SmlI C'TyrA_G
Cuts at: 0 218 2134 3936
Size: 218 1916 1802
Fragments arranged by size:
1916 1802 218
SspI AAT'ATT
Cuts at: 0 2859 3936
Size: 2859 1077
StyI C'CwwG_G
Cuts at: 0 209 440 821 3581 3686 3936
Size: 209 231 381 2760 105 250
Fragments arranged by size:
2760 381 250 231 209 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 425 3728 3936
Size: 425 3303 208
Fragments arranged by size:
3303 425 208
TatI w'GTAC_w
Cuts at: 0 157 198 304 2593 2702 3936
Size: 157 41 106 2289 109 1234
Fragments arranged by size:
2289 1234 157 109 106 41
XmnI GAAnn'nnTTC
Cuts at: 0 572 2576 3254 3936
Size: 572 2004 678 682
Fragments arranged by size:
2004 682 678 572
Enzymes that do cut:
AccI AclI AcuI AflIII AhdI AloI AlwNI ApoI
AseI BanII BbsI BbvCI BcgI BclI BglI BglII
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaBI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrDI BsrGI BstAPI BstYI BtsI ClaI CspCI DraI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII
Hin4I HincII HindIII HpaI MmeI MslI MspA1I NspI
PasI PflMI PfoI PpiI PpuMI PsiI PstI PvuII
SacI SapI SfcI SmlI SspI StyI TaqII TatI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AleI AlfI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI
BanI BbeI BciVI BfrBI BmgBI BmtI BplI BsaI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrBI
BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI DraIII DrdI EaeI EagI EcoNI EcoRI
FseI FspI FspAI KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PmeI PmlI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI