Restriction map for
pF1KB9100
(Linear) (Six-base) MAPSORT of: KIBB9100.seq Check: 9861 from: 1 to: 3324
KIBB9100.orf
With 182 enzymes: SgfI *
March 3, 2008 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1979 3324
Size: 1979 1345
AccI GT'mk_AC
Cuts at: 0 17 3004 3273 3324
Size: 17 2987 269 51
Fragments arranged by size:
2987 269 51 17
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 1438 2455 2656 3324
Size: 136 1302 1017 201 668
Fragments arranged by size:
1302 1017 668 201 136
AflIII A'CryG_T
Cuts at: 0 1394 3324
Size: 1394 1930
AgeI A'CCGG_T
Cuts at: 0 950 3324
Size: 950 2374
AhdI GACnn_n'nnGTC
Cuts at: 0 3021 3324
Size: 3021 303
AleI CACnn'nnGTG
Cuts at: 0 56 3324
Size: 56 3268
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1630 3324
Size: 1630 1694
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 481 513 3324
Size: 481 32 2811
Fragments arranged by size:
2811 481 32
AlwNI CAG_nnn'CTG
Cuts at: 0 361 1440 1790 3294 3324
Size: 361 1079 350 1504 30
Fragments arranged by size:
1504 1079 361 350 30
ApaI G_GGCC'C
Cuts at: 0 2407 2662 3324
Size: 2407 255 662
Fragments arranged by size:
2407 662 255
ApoI r'AATT_y
Cuts at: 0 1891 2122 2554 2614 3324
Size: 1891 231 432 60 710
Fragments arranged by size:
1891 710 432 231 60
AseI AT'TA_AT
Cuts at: 0 166 3324
Size: 166 3158
AvaI C'yCGr_G
Cuts at: 0 720 1052 1082 2041 2096 3324
Size: 720 332 30 959 55 1228
Fragments arranged by size:
1228 959 720 332 55 30
BanI G'GyrC_C
Cuts at: 0 652 1667 3156 3324
Size: 652 1015 1489 168
Fragments arranged by size:
1489 1015 652 168
BanII G_rGCy'C
Cuts at: 0 727 2407 2662 2680 3324
Size: 727 1680 255 18 644
Fragments arranged by size:
1680 727 644 255 18
BbeI G_GCGC'C
Cuts at: 0 656 1671 3324
Size: 656 1015 1653
Fragments arranged by size:
1653 1015 656
BbsI GAAGACnn'nnnn_
Cuts at: 0 2535 2596 3324
Size: 2535 61 728
Fragments arranged by size:
2535 728 61
BciVI GTATCCnnnnn_n'
Cuts at: 0 1542 2872 3324
Size: 1542 1330 452
Fragments arranged by size:
1542 1330 452
BclI T'GATC_A
Cuts at: 0 296 3324
Size: 296 3028
BfrBI ATG'CAT
Cuts at: 0 1410 3324
Size: 1410 1914
BglI GCCn_nnn'nGGC
Cuts at: 0 722 1938 3324
Size: 722 1216 1386
Fragments arranged by size:
1386 1216 722
BglII A'GATC_T
Cuts at: 0 563 3324
Size: 563 2761
Bme1580I G_kGCm'C
Cuts at: 0 2407 2662 3324
Size: 2407 255 662
Fragments arranged by size:
2407 662 255
BmgBI CAC'GTC
Cuts at: 0 1395 3324
Size: 1395 1929
BmrI ACTGGGnnnn_n'
Cuts at: 0 1800 2664 2726 2845 3324
Size: 1800 864 62 119 479
Fragments arranged by size:
1800 864 479 119 62
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 672 2368 3324
Size: 672 1696 956
Fragments arranged by size:
1696 956 672
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 419 478 483 1429 2245 2685 3324
Size: 372 47 59 5 946 816 440 639
Fragments arranged by size:
946 816 639 440 372 59 47 5
Bpu10I CC'TnA_GC
Cuts at: 0 712 2560 3191 3324
Size: 712 1848 631 133
Fragments arranged by size:
1848 712 631 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2710 3037 3324
Size: 2710 327 287
Fragments arranged by size:
2710 327 287
BsaI GGTCTCn'nnnn_
Cuts at: 0 2339 3324
Size: 2339 985
BsaAI yAC'GTr
Cuts at: 0 423 1020 3324
Size: 423 597 2304
Fragments arranged by size:
2304 597 423
BsaBI GATnn'nnATC
Cuts at: 0 796 3324
Size: 796 2528
BsaHI Gr'CG_yC
Cuts at: 0 653 1154 1668 1961 3031 3324
Size: 653 501 514 293 1070 293
Fragments arranged by size:
1070 653 514 501 293 293
BsaWI w'CCGG_w
Cuts at: 0 485 950 1638 3324
Size: 485 465 688 1686
Fragments arranged by size:
1686 688 485 465
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 481 511 2190 2220 3324
Size: 481 30 1679 30 1104
Fragments arranged by size:
1679 1104 481 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 416 1963 2031 2599 2858 3324
Size: 416 1547 68 568 259 466
Fragments arranged by size:
1547 568 466 416 259 68
BseYI C'CCAG_C
Cuts at: 0 1522 2010 3111 3134 3324
Size: 1522 488 1101 23 190
Fragments arranged by size:
1522 1101 488 190 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1060 1951 2690 2710 3324
Size: 760 300 891 739 20 614
Fragments arranged by size:
891 760 739 614 300 20
BsiHKAI G_wGCw'C
Cuts at: 0 1481 1723 2252 3324
Size: 1481 242 529 1072
Fragments arranged by size:
1481 1072 529 242
Bsp1286I G_dGCh'C
Cuts at: 0 727 1481 1723 2252 2407 2662 2680 3324
Size: 727 754 242 529 155 255 18 644
Fragments arranged by size:
754 727 644 529 255 242 155 18
BspEI T'CCGG_A
Cuts at: 0 485 1638 3324
Size: 485 1153 1686
Fragments arranged by size:
1686 1153 485
BspHI T'CATG_A
Cuts at: 0 374 1376 1473 3324
Size: 374 1002 97 1851
Fragments arranged by size:
1851 1002 374 97
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1979 2357 2739 3324
Size: 1979 378 382 585
Fragments arranged by size:
1979 585 382 378
BsrBI CCG'CTC
Cuts at: 0 235 618 3324
Size: 235 383 2706
Fragments arranged by size:
2706 383 235
BsrFI r'CCGG_y
Cuts at: 0 950 974 3324
Size: 950 24 2350
Fragments arranged by size:
2350 950 24
BsrGI T'GTAC_A
Cuts at: 0 284 2963 3324
Size: 284 2679 361
Fragments arranged by size:
2679 361 284
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1974 3324
Size: 1974 1350
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1192 2707 2932 3324
Size: 1192 1515 225 392
Fragments arranged by size:
1515 1192 392 225
BstYI r'GATC_y
Cuts at: 0 157 563 797 1584 2459 3249 3324
Size: 157 406 234 787 875 790 75
Fragments arranged by size:
875 790 787 406 234 157 75
Bsu36I CC'TnA_GG
Cuts at: 0 3152 3324
Size: 3152 172
BtgI C'CryG_G
Cuts at: 0 1121 2972 3324
Size: 1121 1851 352
Fragments arranged by size:
1851 1121 352
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 36 71 3324
Size: 36 35 3253
Fragments arranged by size:
3253 36 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2719 3324
Size: 2719 605
EarI CTCTTCn'nnn_
Cuts at: 0 115 803 899 1851 2057 2240 3324
Size: 115 688 96 952 206 183 1084
Fragments arranged by size:
1084 952 688 206 183 115 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 372 419 478 483 1429 1438 2245
Size: 136 236 47 59 5 946 9 807
Cuts at: 2245 2455 2656 2685 3324
Size: 210 201 29 639
Fragments arranged by size:
946 807 639 236 210 201 136 59
47 29 9 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2873 3324
Size: 2873 451
EcoO109I rG'GnC_Cy
Cuts at: 0 1071 2403 2415 2658 2659 2712 3057 3291
Size: 1071 1332 12 243 1 53 345 234
Cuts at: 3291 3324
Size: 33
Fragments arranged by size:
1332 1071 345 243 234 53 33 12
1
EcoRI G'AATT_C
Cuts at: 0 2554 3324
Size: 2554 770
HaeII r_GCGC'y
Cuts at: 0 656 1671 3324
Size: 656 1015 1653
Fragments arranged by size:
1653 1015 656
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1455 1487 2378 2410 2934 2966 3007 3039
Size: 1455 32 891 32 524 32 41 32
Cuts at: 3039 3324
Size: 285
Fragments arranged by size:
1455 891 524 285 41 32 32 32
32
HincII GTy'rAC
Cuts at: 0 177 1986 3274 3324
Size: 177 1809 1288 50
Fragments arranged by size:
1809 1288 177 50
KasI G'GCGC_C
Cuts at: 0 652 1667 3324
Size: 652 1015 1657
Fragments arranged by size:
1657 1015 652
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 470 593 1471 3324
Size: 470 123 878 1853
Fragments arranged by size:
1853 878 470 123
MslI CAynn'nnrTG
Cuts at: 0 56 263 691 787 1018 1517 1726 1849
Size: 56 207 428 96 231 499 209 123
Cuts at: 1849 2971 3324
Size: 1122 353
Fragments arranged by size:
1122 499 428 353 231 209 207 123
96 56
MspA1I CmG'CkG
Cuts at: 0 453 2010 3324
Size: 453 1557 1314
Fragments arranged by size:
1557 1314 453
NaeI GCC'GGC
Cuts at: 0 976 3324
Size: 976 2348
NarI GG'CG_CC
Cuts at: 0 653 1668 3324
Size: 653 1015 1656
Fragments arranged by size:
1656 1015 653
NcoI C'CATG_G
Cuts at: 0 1121 2972 3324
Size: 1121 1851 352
Fragments arranged by size:
1851 1121 352
NgoMIV G'CCGG_C
Cuts at: 0 974 3324
Size: 974 2350
NsiI A_TGCA'T
Cuts at: 0 1412 3324
Size: 1412 1912
NspI r_CATG'y
Cuts at: 0 1414 3324
Size: 1414 1910
PflMI CCAn_nnn'nTGG
Cuts at: 0 202 556 1790 3324
Size: 202 354 1234 1534
Fragments arranged by size:
1534 1234 354 202
PfoI T'CCnGG_A
Cuts at: 0 1385 2931 3059 3324
Size: 1385 1546 128 265
Fragments arranged by size:
1546 1385 265 128
PmlI CAC'GTG
Cuts at: 0 423 1020 3324
Size: 423 597 2304
Fragments arranged by size:
2304 597 423
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 481 513 3324
Size: 481 32 2811
Fragments arranged by size:
2811 481 32
PpuMI rG'GwC_Cy
Cuts at: 0 2712 3057 3324
Size: 2712 345 267
Fragments arranged by size:
2712 345 267
PshAI GACnn'nnGTC
Cuts at: 0 412 3324
Size: 412 2912
PspOMI G'GGCC_C
Cuts at: 0 2403 2658 3324
Size: 2403 255 666
Fragments arranged by size:
2403 666 255
PspXI vC'TCGA_Gb
Cuts at: 0 2096 3324
Size: 2096 1228
PstI C_TGCA'G
Cuts at: 0 443 663 1238 1652 1979 2750 3078 3324
Size: 443 220 575 414 327 771 328 246
Fragments arranged by size:
771 575 443 414 328 327 246 220
PvuII CAG'CTG
Cuts at: 0 2010 3324
Size: 2010 1314
SalI G'TCGA_C
Cuts at: 0 3272 3324
Size: 3272 52
SapI GCTCTTCn'nnn_
Cuts at: 0 803 1851 2240 3324
Size: 803 1048 389 1084
Fragments arranged by size:
1084 1048 803 389
ScaI AGT'ACT
Cuts at: 0 1318 2481 3324
Size: 1318 1163 843
Fragments arranged by size:
1318 1163 843
SfcI C'TryA_G
Cuts at: 0 439 659 1234 1648 1897 1975 2746 3074
Size: 439 220 575 414 249 78 771 328
Cuts at: 3074 3324
Size: 250
Fragments arranged by size:
771 575 439 414 328 250 249 220
78
SfoI GGC'GCC
Cuts at: 0 654 1669 3324
Size: 654 1015 1655
Fragments arranged by size:
1655 1015 654
SmaI CCC'GGG
Cuts at: 0 1054 1084 3324
Size: 1054 30 2240
Fragments arranged by size:
2240 1054 30
SmlI C'TyrA_G
Cuts at: 0 2096 2725 3052 3324
Size: 2096 629 327 272
Fragments arranged by size:
2096 629 327 272
SphI G_CATG'C
Cuts at: 0 1414 3324
Size: 1414 1910
SspI AAT'ATT
Cuts at: 0 1181 1825 3324
Size: 1181 644 1499
Fragments arranged by size:
1499 1181 644
StuI AGG'CCT
Cuts at: 0 463 1042 3324
Size: 463 579 2282
Fragments arranged by size:
2282 579 463
StyI C'CwwG_G
Cuts at: 0 228 678 1067 1121 1497 2700 2972 3324
Size: 228 450 389 54 376 1203 272 352
Fragments arranged by size:
1203 450 389 376 352 272 228 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1323 1408 1971 2267 3032 3324
Size: 1323 85 563 296 765 292
Fragments arranged by size:
1323 765 563 296 292 85
TatI w'GTAC_w
Cuts at: 0 284 1316 2372 2479 2963 3324
Size: 284 1032 1056 107 484 361
Fragments arranged by size:
1056 1032 484 361 284 107
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 341 373 976 1008 1381 1413 3324
Size: 341 32 603 32 373 32 1911
Fragments arranged by size:
1911 603 373 341 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2305 3324
Size: 2305 1019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 453 576 2704 3324
Size: 453 123 2128 620
Fragments arranged by size:
2128 620 453 123
XhoI C'TCGA_G
Cuts at: 0 2096 3324
Size: 2096 1228
XmaI C'CCGG_G
Cuts at: 0 1052 1082 3324
Size: 1052 30 2242
Fragments arranged by size:
2242 1052 30
XmnI GAAnn'nnTTC
Cuts at: 0 1283 3324
Size: 1283 2041
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AhdI AleI AlfI
AloI AlwNI ApaI ApoI AseI AvaI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BstAPI
BstXI BstYI Bsu36I BtgI CspCI DrdI EarI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I HincII KasI MmeI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI
PstI PvuII SalI SapI ScaI SfcI SfoI SmaI
SmlI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII ApaLI AscI AsiSI
AvrII BaeI BamHI BbvCI BcgI BlpI BmtI BsiEI
BsiWI BsmI BsmBI BsrDI BssHII BssSI BstBI BstEII
BstZ17I BtgZI BtsI ClaI DraI DraIII EaeI EagI
EciI EcoICRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MscI NdeI NheI NotI
NruI PacI PasI PciI PmeI PsiI PsrI PvuI
RsrII SacI SacII SanDI SbfI SexAI SfiI SgfI
SgrAI SnaBI SpeI SrfI SwaI XbaI ZraI