Restriction map for
pF1KB9055
(Linear) (Six-base) MAPSORT of: KIBB9055.seq Check: 1486 from: 1 to: 2934
KIBB9055.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
Acc65I G'GTAC_C
Cuts at: 0 399 2934
Size: 399 2535
AclI AA'CG_TT
Cuts at: 0 2072 2934
Size: 2072 862
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 824 881 1169 1280 1415 1607 2934
Size: 761 63 57 288 111 135 192 1327
Fragments arranged by size:
1327 761 288 192 135 111 63 57
AflIII A'CryG_T
Cuts at: 0 158 831 1264 2934
Size: 158 673 433 1670
Fragments arranged by size:
1670 673 433 158
AlwNI CAG_nnn'CTG
Cuts at: 0 610 2934
Size: 610 2324
ApoI r'AATT_y
Cuts at: 0 1154 1184 1257 1369 1420 1543 1684 1814
Size: 1154 30 73 112 51 123 141 130
Cuts at: 1814 2424 2575 2749 2934
Size: 610 151 174 185
Fragments arranged by size:
1154 610 185 174 151 141 130 123
112 73 51 30
AseI AT'TA_AT
Cuts at: 0 2786 2934
Size: 2786 148
AvaI C'yCGr_G
Cuts at: 0 88 484 2670 2934
Size: 88 396 2186 264
Fragments arranged by size:
2186 396 264 88
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 383 416 2934
Size: 383 33 2518
Fragments arranged by size:
2518 383 33
BanI G'GyrC_C
Cuts at: 0 399 2208 2934
Size: 399 1809 726
Fragments arranged by size:
1809 726 399
BanII G_rGCy'C
Cuts at: 0 2410 2934
Size: 2410 524
BbsI GAAGACnn'nnnn_
Cuts at: 0 2246 2289 2471 2934
Size: 2246 43 182 463
Fragments arranged by size:
2246 463 182 43
BbvCI CC'TCA_GC
Cuts at: 0 2034 2934
Size: 2034 900
BclI T'GATC_A
Cuts at: 0 2802 2934
Size: 2802 132
BfrBI ATG'CAT
Cuts at: 0 270 2934
Size: 270 2664
BglI GCCn_nnn'nGGC
Cuts at: 0 130 2934
Size: 130 2804
BglII A'GATC_T
Cuts at: 0 1125 2934
Size: 1125 1809
BmgBI CAC'GTC
Cuts at: 0 159 187 2934
Size: 159 28 2747
Fragments arranged by size:
2747 159 28
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 63 2412 2934
Size: 63 2349 522
Fragments arranged by size:
2349 522 63
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 1216 2332 2934
Size: 614 602 1116 602
Fragments arranged by size:
1116 614 602 602
Bpu10I CC'TnA_GC
Cuts at: 0 2034 2934
Size: 2034 900
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1046 1676 2934
Size: 1046 630 1258
Fragments arranged by size:
1258 1046 630
BsaI GGTCTCn'nnnn_
Cuts at: 0 422 549 903 2934
Size: 422 127 354 2031
Fragments arranged by size:
2031 422 354 127
BsaAI yAC'GTr
Cuts at: 0 1024 1267 1449 2930 2934
Size: 1024 243 182 1481 4
Fragments arranged by size:
1481 1024 243 182 4
BsaBI GATnn'nnATC
Cuts at: 0 743 2934
Size: 743 2191
BsaWI w'CCGG_w
Cuts at: 0 459 1083 2934
Size: 459 624 1851
Fragments arranged by size:
1851 624 459
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1744 1774 2934
Size: 1744 30 1160
Fragments arranged by size:
1744 1160 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 2388 2934
Size: 55 2333 546
Fragments arranged by size:
2333 546 55
BseYI C'CCAG_C
Cuts at: 0 961 2692 2934
Size: 961 1731 242
Fragments arranged by size:
1731 961 242
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 2934
Size: 469 2465
BsiHKAI G_wGCw'C
Cuts at: 0 1393 2395 2934
Size: 1393 1002 539
Fragments arranged by size:
1393 1002 539
BsmI GAATG_Cn'
Cuts at: 0 1008 2124 2300 2934
Size: 1008 1116 176 634
Fragments arranged by size:
1116 1008 634 176
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2368 2934
Size: 2368 566
Bsp1286I G_dGCh'C
Cuts at: 0 1393 2395 2410 2934
Size: 1393 1002 15 524
Fragments arranged by size:
1393 1002 524 15
BspEI T'CCGG_A
Cuts at: 0 459 2934
Size: 459 2475
BsrDI GCAATG_nn'
Cuts at: 0 20 137 792 2934
Size: 20 117 655 2142
Fragments arranged by size:
2142 655 117 20
BssSI C'ACGA_G
Cuts at: 0 2087 2934
Size: 2087 847
BstBI TT'CG_AA
Cuts at: 0 2043 2934
Size: 2043 891
BstEII G'GTnAC_C
Cuts at: 0 1995 2934
Size: 1995 939
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2700 2934
Size: 2700 234
BstYI r'GATC_y
Cuts at: 0 732 1125 2723 2934
Size: 732 393 1598 211
Fragments arranged by size:
1598 732 393 211
Bsu36I CC'TnA_GG
Cuts at: 0 2264 2934
Size: 2264 670
BtgI C'CryG_G
Cuts at: 0 1839 2000 2934
Size: 1839 161 934
Fragments arranged by size:
1839 934 161
BtsI GCAGTG_nn'
Cuts at: 0 149 2934
Size: 149 2785
DraI TTT'AAA
Cuts at: 0 477 2934
Size: 477 2457
DrdI GACnn_nn'nnGTC
Cuts at: 0 163 1958 2934
Size: 163 1795 976
Fragments arranged by size:
1795 976 163
EaeI y'GGCC_r
Cuts at: 0 110 2934
Size: 110 2824
EarI CTCTTCn'nnn_
Cuts at: 0 1661 2164 2934
Size: 1661 503 770
Fragments arranged by size:
1661 770 503
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 761 824 881 1169 1216 1280 1415
Size: 614 147 63 57 288 47 64 135
Cuts at: 1415 1607 2332 2934
Size: 192 725 602
Fragments arranged by size:
725 614 602 288 192 147 135 64
63 57 47
EcoO109I rG'GnC_Cy
Cuts at: 0 855 2008 2821 2934
Size: 855 1153 813 113
Fragments arranged by size:
1153 855 813 113
EcoRI G'AATT_C
Cuts at: 0 1154 1184 1257 1814 2934
Size: 1154 30 73 557 1120
Fragments arranged by size:
1154 1120 557 73 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 1335 1367 2884 2916 2934
Size: 28 32 1275 32 1517 32 18
Fragments arranged by size:
1517 1275 32 32 32 28 18
HindIII A'AGCT_T
Cuts at: 0 377 1931 2934
Size: 377 1554 1003
Fragments arranged by size:
1554 1003 377
KpnI G_GTAC'C
Cuts at: 0 403 2934
Size: 403 2531
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 2551 2837 2934
Size: 150 2401 286 97
Fragments arranged by size:
2401 286 150 97
MscI TGG'CCA
Cuts at: 0 112 2934
Size: 112 2822
MslI CAynn'nnrTG
Cuts at: 0 142 584 655 799 1436 1525 2482 2934
Size: 142 442 71 144 637 89 957 452
Fragments arranged by size:
957 637 452 442 144 142 89 71
MspA1I CmG'CkG
Cuts at: 0 706 1841 2038 2796 2842 2934
Size: 706 1135 197 758 46 92
Fragments arranged by size:
1135 758 706 197 92 46
NdeI CA'TA_TG
Cuts at: 0 411 2934
Size: 411 2523
NsiI A_TGCA'T
Cuts at: 0 272 2934
Size: 272 2662
NspI r_CATG'y
Cuts at: 0 583 835 2667 2934
Size: 583 252 1832 267
Fragments arranged by size:
1832 583 267 252
PciI A'CATG_T
Cuts at: 0 831 2934
Size: 831 2103
PfoI T'CCnGG_A
Cuts at: 0 944 2234 2772 2934
Size: 944 1290 538 162
Fragments arranged by size:
1290 944 538 162
PmlI CAC'GTG
Cuts at: 0 1267 2930 2934
Size: 1267 1663 4
Fragments arranged by size:
1663 1267 4
PpuMI rG'GwC_Cy
Cuts at: 0 855 2934
Size: 855 2079
PstI C_TGCA'G
Cuts at: 0 264 2934
Size: 264 2670
PvuII CAG'CTG
Cuts at: 0 706 2038 2796 2842 2934
Size: 706 1332 758 46 92
Fragments arranged by size:
1332 758 706 92 46
SacII CC_GC'GG
Cuts at: 0 1842 2934
Size: 1842 1092
SexAI A'CCwGG_T
Cuts at: 0 1804 2934
Size: 1804 1130
SfcI C'TryA_G
Cuts at: 0 260 317 2619 2934
Size: 260 57 2302 315
Fragments arranged by size:
2302 315 260 57
SmaI CCC'GGG
Cuts at: 0 2672 2934
Size: 2672 262
SmlI C'TyrA_G
Cuts at: 0 88 1061 1691 2934
Size: 88 973 630 1243
Fragments arranged by size:
1243 973 630 88
SnaBI TAC'GTA
Cuts at: 0 1024 2934
Size: 1024 1910
SpeI A'CTAG_T
Cuts at: 0 1881 2934
Size: 1881 1053
SphI G_CATG'C
Cuts at: 0 2667 2934
Size: 2667 267
SspI AAT'ATT
Cuts at: 0 235 2934
Size: 235 2699
StuI AGG'CCT
Cuts at: 0 2113 2934
Size: 2113 821
StyI C'CwwG_G
Cuts at: 0 1382 1400 1504 2934
Size: 1382 18 104 1430
Fragments arranged by size:
1430 1382 104 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2448 2934
Size: 2448 486
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2565 2597 2934
Size: 2565 32 337
Fragments arranged by size:
2565 337 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 126 2927 2934
Size: 126 2801 7
Fragments arranged by size:
2801 126 7
XhoI C'TCGA_G
Cuts at: 0 88 2934
Size: 88 2846
XmaI C'CCGG_G
Cuts at: 0 2670 2934
Size: 2670 264
XmnI GAAnn'nnTTC
Cuts at: 0 1142 1154 1223 1469 2934
Size: 1142 12 69 246 1465
Fragments arranged by size:
1465 1142 246 69 12
Enzymes that do cut:
Acc65I AclI AcuI AflIII AlwNI ApoI AseI AvaI
BaeI BanI BanII BbsI BbvCI BclI BfrBI BglI
BglII BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BsrDI BssSI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraI DrdI EaeI EarI
Eco57MI EcoO109I EcoRI Hin4I HindIII KpnI MmeI MscI
MslI MspA1I NdeI NsiI NspI PciI PfoI PmlI
PpuMI PstI PvuII SacII SexAI SfcI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TstI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BamHI
BbeI BcgI BciVI BlpI Bme1580I BmrI BmtI BsaHI
BsiEI BsiWI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BstAPI BstZ17I BtgZI ClaI CspCI DraIII EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HincII HpaI KasI MfeI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI PacI PasI PflMI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SrfI SwaI TatI Tth111I XbaI ZraI