Restriction map for
pF1KB9044
(Linear) (Six-base) MAPSORT of: KIBB9044.seq Check: 3919 from: 1 to: 1590
KIBB9044.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 1590
Size: 293 1297
AgeI A'CCGG_T
Cuts at: 0 1499 1590
Size: 1499 91
ApoI r'AATT_y
Cuts at: 0 61 105 354 493 501 605 621 689
Size: 61 44 249 139 8 104 16 68
Cuts at: 689 773 941 1025 1109 1193 1277 1445 1529
Size: 84 168 84 84 84 84 168 84
Cuts at: 1529 1590
Size: 61
Fragments arranged by size:
249 168 168 139 104 84 84 84
84 84 84 68 61 61 44 16
8
BanII G_rGCy'C
Cuts at: 0 212 1590
Size: 212 1378
BbsI GAAGACnn'nnnn_
Cuts at: 0 141 281 1590
Size: 141 140 1309
Fragments arranged by size:
1309 141 140
BmrI ACTGGGnnnn_n'
Cuts at: 0 244 1590
Size: 244 1346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 887 971 1055 1139 1223 1239 1391
Size: 91 796 84 84 84 84 16 152
Cuts at: 1391 1590
Size: 199
Fragments arranged by size:
796 199 152 91 84 84 84 84
16
BsaAI yAC'GTr
Cuts at: 0 477 1590
Size: 477 1113
BsaWI w'CCGG_w
Cuts at: 0 1499 1590
Size: 1499 91
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 1590
Size: 88 1502
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 1590
Size: 80 1510
BsiHKAI G_wGCw'C
Cuts at: 0 1255 1590
Size: 1255 335
BsmI GAATG_Cn'
Cuts at: 0 1150 1590
Size: 1150 440
Bsp1286I G_dGCh'C
Cuts at: 0 212 1255 1590
Size: 212 1043 335
Fragments arranged by size:
1043 335 212
BsrDI GCAATG_nn'
Cuts at: 0 88 1410 1590
Size: 88 1322 180
Fragments arranged by size:
1322 180 88
BsrFI r'CCGG_y
Cuts at: 0 1499 1590
Size: 1499 91
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1429 1590
Size: 1429 161
BtsI GCAGTG_nn'
Cuts at: 0 92 1590
Size: 92 1498
EaeI y'GGCC_r
Cuts at: 0 53 283 1590
Size: 53 230 1307
Fragments arranged by size:
1307 230 53
EarI CTCTTCn'nnn_
Cuts at: 0 217 779 1590
Size: 217 562 811
Fragments arranged by size:
811 562 217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 293 887 971 1055 1139 1223 1239
Size: 91 202 594 84 84 84 84 16
Cuts at: 1239 1391 1590
Size: 152 199
Fragments arranged by size:
594 202 199 152 91 84 84 84
84 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 216 1590
Size: 216 1374
EcoRI G'AATT_C
Cuts at: 0 61 605 621 689 941 1193 1277 1445
Size: 61 544 16 68 252 252 84 168
Cuts at: 1445 1590
Size: 145
Fragments arranged by size:
544 252 252 168 145 84 68 61
16
HindIII A'AGCT_T
Cuts at: 0 1070 1154 1238 1490 1590
Size: 1070 84 84 252 100
Fragments arranged by size:
1070 252 100 84 84
MscI TGG'CCA
Cuts at: 0 55 285 1590
Size: 55 230 1305
Fragments arranged by size:
1305 230 55
MslI CAynn'nnrTG
Cuts at: 0 1538 1590
Size: 1538 52
NdeI CA'TA_TG
Cuts at: 0 695 1187 1590
Size: 695 492 403
Fragments arranged by size:
695 492 403
NspI r_CATG'y
Cuts at: 0 579 1590
Size: 579 1011
PsiI TTA'TAA
Cuts at: 0 416 1590
Size: 416 1174
SexAI A'CCwGG_T
Cuts at: 0 143 1590
Size: 143 1447
SfcI C'TryA_G
Cuts at: 0 135 1590
Size: 135 1455
SphI G_CATG'C
Cuts at: 0 579 1590
Size: 579 1011
SspI AAT'ATT
Cuts at: 0 458 1590
Size: 458 1132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1238 1590
Size: 1238 352
XmnI GAAnn'nnTTC
Cuts at: 0 541 793 961 1590
Size: 541 252 168 629
Fragments arranged by size:
629 541 252 168
Enzymes that do cut:
AcuI AgeI ApoI BanII BbsI BmrI BpmI BsaAI
BsaWI BseRI BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrFI
BstAPI BtsI EaeI EarI Eco57MI EcoNI EcoRI HindIII
MscI MslI NdeI NspI PsiI SexAI SfcI SphI
SspI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoO109I EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SrfI StuI StyI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI