Restriction map for
pF1KB9038
(Linear) (Six-base) MAPSORT of: KIBB9038.seq Check: 1039 from: 1 to: 1332
KIBB9038.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 721 1332
Size: 721 611
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 664 1332
Size: 483 181 668
Fragments arranged by size:
668 483 181
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1172 1332
Size: 1172 160
AlwNI CAG_nnn'CTG
Cuts at: 0 433 1332
Size: 433 899
ApaLI G'TGCA_C
Cuts at: 0 13 1332
Size: 13 1319
BamHI G'GATC_C
Cuts at: 0 1031 1332
Size: 1031 301
BanI G'GyrC_C
Cuts at: 0 49 100 325 507 1332
Size: 49 51 225 182 825
Fragments arranged by size:
825 225 182 51 49
BanII G_rGCy'C
Cuts at: 0 545 849 1130 1332
Size: 545 304 281 202
Fragments arranged by size:
545 304 281 202
BbeI G_GCGC'C
Cuts at: 0 329 1332
Size: 329 1003
BbsI GAAGACnn'nnnn_
Cuts at: 0 870 939 1332
Size: 870 69 393
Fragments arranged by size:
870 393 69
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 27 61 1332
Size: 27 34 1271
Fragments arranged by size:
1271 34 27
BclI T'GATC_A
Cuts at: 0 68 1332
Size: 68 1264
BglI GCCn_nnn'nGGC
Cuts at: 0 392 1332
Size: 392 940
BglII A'GATC_T
Cuts at: 0 248 1332
Size: 248 1084
Bme1580I G_kGCm'C
Cuts at: 0 17 427 445 1332
Size: 17 410 18 887
Fragments arranged by size:
887 410 18 17
BmgBI CAC'GTC
Cuts at: 0 784 1332
Size: 784 548
BmrI ACTGGGnnnn_n'
Cuts at: 0 309 424 1332
Size: 309 115 908
Fragments arranged by size:
908 309 115
Bpu10I CC'TnA_GC
Cuts at: 0 231 1332
Size: 231 1101
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1332
Size: 1066 266
BsaI GGTCTCn'nnnn_
Cuts at: 0 586 1332
Size: 586 746
BsaHI Gr'CG_yC
Cuts at: 0 326 1332
Size: 326 1006
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1309 1312 1327 1332
Size: 1309 3 15 5
Fragments arranged by size:
1309 15 5 3
BseYI C'CCAG_C
Cuts at: 0 869 1332
Size: 869 463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1018 1332
Size: 1018 314
BsiEI CG_ry'CG
Cuts at: 0 924 1332
Size: 924 408
BsiHKAI G_wGCw'C
Cuts at: 0 17 849 1130 1332
Size: 17 832 281 202
Fragments arranged by size:
832 281 202 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 790 1260 1332
Size: 790 470 72
Fragments arranged by size:
790 470 72
Bsp1286I G_dGCh'C
Cuts at: 0 17 427 445 545 849 1130 1332
Size: 17 410 18 100 304 281 202
Fragments arranged by size:
410 304 281 202 100 18 17
BspHI T'CATG_A
Cuts at: 0 488 1332
Size: 488 844
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 131 280 721 864 1332
Size: 131 149 441 143 468
Fragments arranged by size:
468 441 149 143 131
BsrFI r'CCGG_y
Cuts at: 0 97 1332
Size: 97 1235
BssSI C'ACGA_G
Cuts at: 0 182 1073 1332
Size: 182 891 259
Fragments arranged by size:
891 259 182
BstXI CCAn_nnnn'nTGG
Cuts at: 0 195 1332
Size: 195 1137
BstYI r'GATC_y
Cuts at: 0 198 248 461 669 1031 1332
Size: 198 50 213 208 362 301
Fragments arranged by size:
362 301 213 208 198 50
BtgI C'CryG_G
Cuts at: 0 108 215 533 825 918 935 951 1332
Size: 108 107 318 292 93 17 16 381
Fragments arranged by size:
381 318 292 108 107 93 17 16
BtsI GCAGTG_nn'
Cuts at: 0 1154 1332
Size: 1154 178
DraIII CAC_nnn'GTG
Cuts at: 0 1075 1332
Size: 1075 257
DrdI GACnn_nn'nnGTC
Cuts at: 0 535 1332
Size: 535 797
EaeI y'GGCC_r
Cuts at: 0 243 729 921 1332
Size: 243 486 192 411
Fragments arranged by size:
486 411 243 192
EagI C'GGCC_G
Cuts at: 0 921 1332
Size: 921 411
EarI CTCTTCn'nnn_
Cuts at: 0 1135 1286 1316 1332
Size: 1135 151 30 16
Fragments arranged by size:
1135 151 30 16
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1156 1332
Size: 1156 176
EcoICRI GAG'CTC
Cuts at: 0 847 1128 1332
Size: 847 281 204
Fragments arranged by size:
847 281 204
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 664 1332
Size: 483 181 668
Fragments arranged by size:
668 483 181
EcoO109I rG'GnC_Cy
Cuts at: 0 235 615 1332
Size: 235 380 717
Fragments arranged by size:
717 380 235
EcoRV GAT'ATC
Cuts at: 0 627 1332
Size: 627 705
HaeII r_GCGC'y
Cuts at: 0 329 1332
Size: 329 1003
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 1240 1272 1332
Size: 124 32 1084 32 60
Fragments arranged by size:
1084 124 60 32 32
HincII GTy'rAC
Cuts at: 0 765 1332
Size: 765 567
KasI G'GCGC_C
Cuts at: 0 325 1332
Size: 325 1007
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1332
Size: 937 395
MscI TGG'CCA
Cuts at: 0 245 731 1332
Size: 245 486 601
Fragments arranged by size:
601 486 245
MslI CAynn'nnrTG
Cuts at: 0 142 193 1159 1332
Size: 142 51 966 173
Fragments arranged by size:
966 173 142 51
MspA1I CmG'CkG
Cuts at: 0 129 402 1332
Size: 129 273 930
Fragments arranged by size:
930 273 129
NarI GG'CG_CC
Cuts at: 0 326 1332
Size: 326 1006
NcoI C'CATG_G
Cuts at: 0 215 1332
Size: 215 1117
PflMI CCAn_nnn'nTGG
Cuts at: 0 59 414 573 1075 1332
Size: 59 355 159 502 257
Fragments arranged by size:
502 355 257 159 59
PfoI T'CCnGG_A
Cuts at: 0 347 1121 1332
Size: 347 774 211
Fragments arranged by size:
774 347 211
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1204 1236 1332
Size: 1204 32 96
Fragments arranged by size:
1204 96 32
PpuMI rG'GwC_Cy
Cuts at: 0 235 1332
Size: 235 1097
PshAI GACnn'nnGTC
Cuts at: 0 1054 1332
Size: 1054 278
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 1332
Size: 1000 32 300
Fragments arranged by size:
1000 300 32
PstI C_TGCA'G
Cuts at: 0 128 392 1332
Size: 128 264 940
Fragments arranged by size:
940 264 128
PvuII CAG'CTG
Cuts at: 0 129 402 1332
Size: 129 273 930
Fragments arranged by size:
930 273 129
SacI G_AGCT'C
Cuts at: 0 849 1130 1332
Size: 849 281 202
Fragments arranged by size:
849 281 202
SapI GCTCTTCn'nnn_
Cuts at: 0 1135 1332
Size: 1135 197
SbfI CC_TGCA'GG
Cuts at: 0 392 1332
Size: 392 940
SfcI C'TryA_G
Cuts at: 0 124 388 1332
Size: 124 264 944
Fragments arranged by size:
944 264 124
SfoI GGC'GCC
Cuts at: 0 327 1332
Size: 327 1005
SmlI C'TyrA_G
Cuts at: 0 1081 1332
Size: 1081 251
StuI AGG'CCT
Cuts at: 0 1177 1332
Size: 1177 155
StyI C'CwwG_G
Cuts at: 0 215 1332
Size: 215 1117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 531 883 1332
Size: 531 352 449
Fragments arranged by size:
531 449 352
TatI w'GTAC_w
Cuts at: 0 146 1332
Size: 146 1186
Tth111I GACn'n_nGTC
Cuts at: 0 1252 1332
Size: 1252 80
XbaI T'CTAG_A
Cuts at: 0 201 1332
Size: 201 1131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 894 1332
Size: 894 438
XmnI GAAnn'nnTTC
Cuts at: 0 496 1177 1332
Size: 496 681 155
Fragments arranged by size:
681 496 155
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaLI BamHI BanI BanII
BbeI BbsI BcgI BclI BglI BglII Bme1580I BmgBI
BmrI Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrFI BssSI
BstXI BstYI BtgI BtsI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NarI NcoI
PflMI PfoI PpiI PpuMI PshAI PsrI PstI PvuII
SacI SapI SbfI SfcI SfoI SmlI StuI StyI
TaqII TatI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbvCI BciVI BfrBI BlpI BmtI
BplI BpmI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI
BspEI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI EcoNI EcoRI
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PsiI PspOMI
PspXI PvuI RsrII SacII SalI SanDI ScaI SexAI
SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI XhoI XmaI ZraI