(Linear) (Six-base) MAPSORT of: KIBB9027.seq  Check: 7120  from: 1  to: 717



KIBB9027.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:38  ..

AgeI A'CCGG_T

Cuts at:      0      42     717 
   Size:         42     675
 
ApoI r'AATT_y

Cuts at:      0     590     717 
   Size:        590     127
 
AseI AT'TA_AT

Cuts at:      0     139     169     717 
   Size:        139      30     548

  Fragments arranged by size: 

                 548     139      30
 
BamHI G'GATC_C

Cuts at:      0     642     717 
   Size:        642      75
 
BclI T'GATC_A

Cuts at:      0     104     717 
   Size:        104     613
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     717 
   Size:         43     674
 
BsaBI GATnn'nnATC

Cuts at:      0     641     717 
   Size:        641      76
 
BsaWI w'CCGG_w

Cuts at:      0      42     717 
   Size:         42     675
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     112     717 
   Size:        112     605
 
BsiHKAI G_wGCw'C

Cuts at:      0     473     717 
   Size:        473     244
 
BsmI GAATG_Cn'

Cuts at:      0     254     717 
   Size:        254     463
 
Bsp1286I G_dGCh'C

Cuts at:      0     473     717 
   Size:        473     244
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      38     717 
   Size:         38     679
 
BsrFI r'CCGG_y

Cuts at:      0      42     717 
   Size:         42     675
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     709     717 
   Size:        709       8
 
BstYI r'GATC_y

Cuts at:      0      35     642     717 
   Size:         35     607      75

  Fragments arranged by size: 

                 607      75      35
 
Bsu36I CC'TnA_GG

Cuts at:      0     665     717 
   Size:        665      52
 
BtsI GCAGTG_nn'

Cuts at:      0      62     717 
   Size:         62     655
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     491     526     717 
   Size:        491      35     191

  Fragments arranged by size: 

                 491     191      35
 
DraI TTT'AAA

Cuts at:      0     147     717 
   Size:        147     570
 
EaeI y'GGCC_r

Cuts at:      0     673     717 
   Size:        673      44
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     717 
   Size:         43     674
 
EcoO109I rG'GnC_Cy

Cuts at:      0     450     717 
   Size:        450     267
 
EcoRI G'AATT_C

Cuts at:      0     590     717 
   Size:        590     127
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      77     180     256     513     717 
   Size:         77     103      76     257     204

  Fragments arranged by size: 

                 257     204     103      77      76
 
MspA1I CmG'CkG

Cuts at:      0     705     717 
   Size:        705      12
 
PasI CC'CwG_GG

Cuts at:      0     464     717 
   Size:        464     253
 
PstI C_TGCA'G

Cuts at:      0      35     717 
   Size:         35     682
 
SexAI A'CCwGG_T

Cuts at:      0     576     717 
   Size:        576     141
 
SfcI C'TryA_G

Cuts at:      0      31     717 
   Size:         31     686
 
StuI AGG'CCT

Cuts at:      0     367     717 
   Size:        367     350
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     456     717 
   Size:        456     261
 
TatI w'GTAC_w

Cuts at:      0     122     717 
   Size:        122     595
 
XmnI GAAnn'nnTTC

Cuts at:      0     427     717 
   Size:        427     290
 

 Enzymes that do cut:

     AgeI     ApoI     AseI    BamHI     BclI     BpmI    BsaBI    BsaWI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrFI    BstXI    BstYI
   Bsu36I     BtsI    CspCI     DraI     EaeI  Eco57MI EcoO109I    EcoRI
     MmeI   MspA1I     PasI     PstI    SexAI     SfcI     StuI    TaqII
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I     BtgI    BtgZI     ClaI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI