Restriction map for
pF1KB9019
(Linear) (Six-base) MAPSORT of: KIBB9019.seq Check: 1304 from: 1 to: 7374
KIBB9019.orf
With 182 enzymes: SgfI *
January 8, 2014 09:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 830 1746 3961 7349 7374
Size: 830 916 2215 3388 25
Fragments arranged by size:
3388 2215 916 830 25
AatII G_ACGT'C
Cuts at: 0 3827 7374
Size: 3827 3547
AccI GT'mk_AC
Cuts at: 0 559 5918 7348 7374
Size: 559 5359 1430 26
Fragments arranged by size:
5359 1430 559 26
AclI AA'CG_TT
Cuts at: 0 2804 7374
Size: 2804 4570
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1046 1388 1867 2034 3082 4098 4179 6190
Size: 1046 342 479 167 1048 1016 81 2011
Cuts at: 6190 6535 7237 7374
Size: 345 702 137
Fragments arranged by size:
2011 1048 1046 1016 702 479 345 342
167 137 81
AflII C'TTAA_G
Cuts at: 0 910 7374
Size: 910 6464
AflIII A'CryG_T
Cuts at: 0 2036 2653 3488 3755 5528 6497 7374
Size: 2036 617 835 267 1773 969 877
Fragments arranged by size:
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AhdI GACnn_n'nnGTC
Cuts at: 0 368 7374
Size: 368 7006
AleI CACnn'nnGTG
Cuts at: 0 3220 3955 4750 7374
Size: 3220 735 795 2624
Fragments arranged by size:
3220 2624 795 735
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2099 7374
Size: 2099 5275
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2003 2035 2159 2191 4600 4632 4720 4752
Size: 2003 32 124 32 2409 32 88 32
Cuts at: 4752 6697 6729 7374
Size: 1945 32 645
Fragments arranged by size:
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32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 151 2013 2496 2952 3501 3681 5133 6325
Size: 151 1862 483 456 549 180 1452 1192
Cuts at: 6325 6334 6513 7152 7374
Size: 9 179 639 222
Fragments arranged by size:
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180 179 151 9
ApaI G_GGCC'C
Cuts at: 0 594 971 5057 7374
Size: 594 377 4086 2317
Fragments arranged by size:
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ApoI r'AATT_y
Cuts at: 0 2016 3964 4140 4408 5471 5859 6103 7374
Size: 2016 1948 176 268 1063 388 244 1271
Fragments arranged by size:
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AseI AT'TA_AT
Cuts at: 0 2449 3796 7374
Size: 2449 1347 3578
Fragments arranged by size:
3578 2449 1347
AvaI C'yCGr_G
Cuts at: 0 126 901 1931 3778 5450 7267 7374
Size: 126 775 1030 1847 1672 1817 107
Fragments arranged by size:
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BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3399 3432 7374
Size: 3399 33 3942
Fragments arranged by size:
3942 3399 33
BamHI G'GATC_C
Cuts at: 0 3362 5398 7374
Size: 3362 2036 1976
Fragments arranged by size:
3362 2036 1976
BanI G'GyrC_C
Cuts at: 0 2845 3098 3949 4314 5494 6225 7374
Size: 2845 253 851 365 1180 731 1149
Fragments arranged by size:
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BanII G_rGCy'C
Cuts at: 0 140 505 594 971 1996 2570 2888 3044
Size: 140 365 89 377 1025 574 318 156
Cuts at: 3044 3626 3672 4058 4256 5057 5200 5731 6123
Size: 582 46 386 198 801 143 531 392
Cuts at: 6123 6596 7374
Size: 473 778
Fragments arranged by size:
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386 377 365 318 198 156 143 140
89 46
BbeI G_GCGC'C
Cuts at: 0 5498 7374
Size: 5498 1876
BbsI GAAGACnn'nnnn_
Cuts at: 0 455 2976 3300 5564 6964 7374
Size: 455 2521 324 2264 1400 410
Fragments arranged by size:
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BbvCI CC'TCA_GC
Cuts at: 0 405 4039 4889 7374
Size: 405 3634 850 2485
Fragments arranged by size:
3634 2485 850 405
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2524 2558 7374
Size: 2524 34 4816
Fragments arranged by size:
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BciVI GTATCCnnnnn_n'
Cuts at: 0 5222 7374
Size: 5222 2152
BclI T'GATC_A
Cuts at: 0 1355 2789 3551 4652 5072 5675 7374
Size: 1355 1434 762 1101 420 603 1699
Fragments arranged by size:
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BfrBI ATG'CAT
Cuts at: 0 5958 7374
Size: 5958 1416
BglI GCCn_nnn'nGGC
Cuts at: 0 1138 1621 7374
Size: 1138 483 5753
Fragments arranged by size:
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BglII A'GATC_T
Cuts at: 0 5011 6726 7374
Size: 5011 1715 648
Fragments arranged by size:
5011 1715 648
BlpI GC'TnA_GC
Cuts at: 0 3634 7319 7374
Size: 3634 3685 55
Fragments arranged by size:
3685 3634 55
Bme1580I G_kGCm'C
Cuts at: 0 594 971 2105 4013 4178 4541 4793 5057
Size: 594 377 1134 1908 165 363 252 264
Cuts at: 5057 7374
Size: 2317
Fragments arranged by size:
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165
BmgBI CAC'GTC
Cuts at: 0 1941 2654 7374
Size: 1941 713 4720
Fragments arranged by size:
4720 1941 713
BmrI ACTGGGnnnn_n'
Cuts at: 0 368 1118 3192 7374
Size: 368 750 2074 4182
Fragments arranged by size:
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BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 246 3624 7374
Size: 246 3378 3750
Fragments arranged by size:
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BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 622 1224 2087 2242 2902 3139 4318
Size: 394 228 602 863 155 660 237 1179
Cuts at: 4318 4729 4747 4992 5089 5432 6040 6489 6844
Size: 411 18 245 97 343 608 449 355
Cuts at: 6844 6943 6970 7024 7374
Size: 99 27 54 350
Fragments arranged by size:
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355 350 343 245 237 228 155 99
97 54 27 18
Bpu10I CC'TnA_GC
Cuts at: 0 405 4039 4889 5730 7297 7374
Size: 405 3634 850 841 1567 77
Fragments arranged by size:
3634 1567 850 841 405 77
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2461 3772 7219 7374
Size: 2461 1311 3447 155
Fragments arranged by size:
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BsaI GGTCTCn'nnnn_
Cuts at: 0 625 2218 7374
Size: 625 1593 5156
Fragments arranged by size:
5156 1593 625
BsaAI yAC'GTr
Cuts at: 0 3888 5478 5538 7056 7211 7374
Size: 3888 1590 60 1518 155 163
Fragments arranged by size:
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BsaBI GATnn'nnATC
Cuts at: 0 1793 3610 4903 5071 5368 6574 7374
Size: 1793 1817 1293 168 297 1206 800
Fragments arranged by size:
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BsaHI Gr'CG_yC
Cuts at: 0 3824 3871 5495 6932 6984 7374
Size: 3824 47 1624 1437 52 390
Fragments arranged by size:
3824 1624 1437 390 52 47
BsaWI w'CCGG_w
Cuts at: 0 878 1080 1391 2021 2304 3998 4508 6481
Size: 878 202 311 630 283 1694 510 1973
Cuts at: 6481 7374
Size: 893
Fragments arranged by size:
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202
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 505 535 1075 1105 2095 2125 3685 3715
Size: 505 30 540 30 990 30 1560 30
Cuts at: 3715 4091 4121 4720 4750 6013 6043 6697 6727
Size: 376 30 599 30 1263 30 654 30
Cuts at: 6727 7374
Size: 647
Fragments arranged by size:
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376 30 30 30 30 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 393 510 4837 5530 5672 6988 7374
Size: 393 117 4327 693 142 1316 386
Fragments arranged by size:
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BseYI C'CCAG_C
Cuts at: 0 211 1049 1484 1808 2412 2704 2752 2942
Size: 211 838 435 324 604 292 48 190
Cuts at: 2942 3238 5057 5743 5832 5937 7314 7374
Size: 296 1819 686 89 105 1377 60
Fragments arranged by size:
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292 211 190 105 89 60 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1051 1706 1755 1822 2059 2311 2959
Size: 697 354 655 49 67 237 252 648
Cuts at: 2959 3094 3355 4307 6904 7165 7374
Size: 135 261 952 2597 261 209
Fragments arranged by size:
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252 237 209 135 67 49
BsiEI CG_ry'CG
Cuts at: 0 258 1805 6915 7374
Size: 258 1547 5110 459
Fragments arranged by size:
5110 1547 459 258
BsiHKAI G_wGCw'C
Cuts at: 0 1996 2888 3425 3626 3650 4058 5892 6123
Size: 1996 892 537 201 24 408 1834 231
Cuts at: 6123 6596 7374
Size: 473 778
Fragments arranged by size:
1996 1834 892 778 537 473 408 231
201 24
BsmI GAATG_Cn'
Cuts at: 0 1539 5462 7374
Size: 1539 3923 1912
Fragments arranged by size:
3923 1912 1539
BsmBI CGTCTCn'nnnn_
Cuts at: 0 7087 7374
Size: 7087 287
Bsp1286I G_dGCh'C
Cuts at: 0 140 505 594 971 1996 2105 2570 2888
Size: 140 365 89 377 1025 109 465 318
Cuts at: 2888 3044 3425 3626 3650 3672 4013 4058 4178
Size: 156 381 201 24 22 341 45 120
Cuts at: 4178 4256 4541 4793 5057 5200 5731 5892 6123
Size: 78 285 252 264 143 531 161 231
Cuts at: 6123 6596 7374
Size: 473 778
Fragments arranged by size:
1025 778 531 473 465 381 377 365
341 318 285 264 252 231 201 161
156 143 140 120 109 89 78 45
24 22
BspEI T'CCGG_A
Cuts at: 0 1080 1391 2021 7374
Size: 1080 311 630 5353
Fragments arranged by size:
5353 1080 630 311
BspHI T'CATG_A
Cuts at: 0 1472 2516 2792 3017 3083 3134 7374
Size: 1472 1044 276 225 66 51 4240
Fragments arranged by size:
4240 1472 1044 276 225 66 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 719 830 1746 3961 4043 4488 4688 5438
Size: 719 111 916 2215 82 445 200 750
Cuts at: 5438 5803 6860 7320 7349 7374
Size: 365 1057 460 29 25
Fragments arranged by size:
2215 1057 916 750 719 460 445 365
200 111 82 29 25
BsrBI CCG'CTC
Cuts at: 0 870 4698 6188 7323 7374
Size: 870 3828 1490 1135 51
Fragments arranged by size:
3828 1490 1135 870 51
BsrDI GCAATG_nn'
Cuts at: 0 1010 1943 2276 5382 7374
Size: 1010 933 333 3106 1992
Fragments arranged by size:
3106 1992 1010 933 333
BsrFI r'CCGG_y
Cuts at: 0 253 1602 1800 1841 2549 3932 4859 4921
Size: 253 1349 198 41 708 1383 927 62
Cuts at: 4921 6830 7374
Size: 1909 544
Fragments arranged by size:
1909 1383 1349 927 708 544 253 198
62 41
BssSI C'ACGA_G
Cuts at: 0 551 2077 7374
Size: 551 1526 5297
Fragments arranged by size:
5297 1526 551
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2952 4763 5067 6325 6334 7374
Size: 2952 1811 304 1258 9 1040
Fragments arranged by size:
2952 1811 1258 1040 304 9
BstBI TT'CG_AA
Cuts at: 0 2143 4673 7374
Size: 2143 2530 2701
Fragments arranged by size:
2701 2530 2143
BstEII G'GTnAC_C
Cuts at: 0 894 5790 7374
Size: 894 4896 1584
Fragments arranged by size:
4896 1584 894
BstXI CCAn_nnnn'nTGG
Cuts at: 0 430 1659 1671 3261 5787 5941 7374
Size: 430 1229 12 1590 2526 154 1433
Fragments arranged by size:
2526 1590 1433 1229 430 154 12
BstYI r'GATC_y
Cuts at: 0 1448 1511 2692 3362 3393 3677 5011 5097
Size: 1448 63 1181 670 31 284 1334 86
Cuts at: 5097 5398 6726 7025 7374
Size: 301 1328 299 349
Fragments arranged by size:
1448 1334 1328 1181 670 349 301 299
284 86 63 31
Bsu36I CC'TnA_GG
Cuts at: 0 1143 3466 7374
Size: 1143 2323 3908
Fragments arranged by size:
3908 2323 1143
BtgI C'CryG_G
Cuts at: 0 728 1670 1823 1879 2198 3366 4103 4715
Size: 728 942 153 56 319 1168 737 612
Cuts at: 4715 6201 6242 6278 7277 7352 7374
Size: 1486 41 36 999 75 22
Fragments arranged by size:
1486 1168 999 942 737 728 612 319
153 75 56 41 36 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1596 2368 3888 7374
Size: 1596 772 1520 3486
Fragments arranged by size:
3486 1596 1520 772
BtsI GCAGTG_nn'
Cuts at: 0 1651 1834 2103 2950 3106 3648 4932 7153
Size: 1651 183 269 847 156 542 1284 2221
Cuts at: 7153 7374
Size: 221
Fragments arranged by size:
2221 1651 1284 847 542 269 221 183
156
ClaI AT'CG_AT
Cuts at: 0 3563 6752 7374
Size: 3563 3189 622
Fragments arranged by size:
3563 3189 622
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 5524 5559 7374
Size: 5524 35 1815
Fragments arranged by size:
5524 1815 35
DraI TTT'AAA
Cuts at: 0 5187 5577 7374
Size: 5187 390 1797
Fragments arranged by size:
5187 1797 390
DraIII CAC_nnn'GTG
Cuts at: 0 678 2261 6876 7374
Size: 678 1583 4615 498
Fragments arranged by size:
4615 1583 678 498
EaeI y'GGCC_r
Cuts at: 0 1604 1622 1661 1802 2057 2345 2873 3530
Size: 1604 18 39 141 255 288 528 657
Cuts at: 3530 3762 6912 7274 7374
Size: 232 3150 362 100
Fragments arranged by size:
3150 1604 657 528 362 288 255 232
141 100 39 18
EagI C'GGCC_G
Cuts at: 0 1802 6912 7374
Size: 1802 5110 462
Fragments arranged by size:
5110 1802 462
EarI CTCTTCn'nnn_
Cuts at: 0 676 2126 2609 4063 4198 4244 5762 5904
Size: 676 1450 483 1454 135 46 1518 142
Cuts at: 5904 7374
Size: 1470
Fragments arranged by size:
1518 1470 1454 1450 676 483 142 135
46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 276 955 1391 1645 2311 4897 4930 7374
Size: 276 679 436 254 666 2586 33 2444
Fragments arranged by size:
2586 2444 679 666 436 276 254 33
EcoICRI GAG'CTC
Cuts at: 0 1994 2886 3624 4056 6121 6594 7374
Size: 1994 892 738 432 2065 473 780
Fragments arranged by size:
2065 1994 892 780 738 473 432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 622 1046 1224 1388 1867 2034 2087
Size: 394 228 424 178 164 479 167 53
Cuts at: 2087 2242 2902 3082 3139 4098 4179 4318 4729
Size: 155 660 180 57 959 81 139 411
Cuts at: 4729 4747 4992 5089 5432 6040 6190 6489 6535
Size: 18 245 97 343 608 150 299 46
Cuts at: 6535 6844 6943 6970 7024 7237 7374
Size: 309 99 27 54 213 137
Fragments arranged by size:
959 660 608 479 424 411 394 343
309 299 245 228 213 180 178 167
164 155 150 139 137 99 97 81
57 54 53 46 27 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 408 7003 7374
Size: 408 6595 371
Fragments arranged by size:
6595 408 371
EcoO109I rG'GnC_Cy
Cuts at: 0 241 413 590 591 906 967 1117 1188
Size: 241 172 177 1 315 61 150 71
Cuts at: 1188 2333 2406 5053 5054 5418 5816 5901 6819
Size: 1145 73 2647 1 364 398 85 918
Cuts at: 6819 6849 7374
Size: 30 525
Fragments arranged by size:
2647 1145 918 525 398 364 315 241
177 172 150 85 73 71 61 30
1 1
EcoRI G'AATT_C
Cuts at: 0 3964 5859 6103 7374
Size: 3964 1895 244 1271
Fragments arranged by size:
3964 1895 1271 244
EcoRV GAT'ATC
Cuts at: 0 1854 2310 5685 7374
Size: 1854 456 3375 1689
Fragments arranged by size:
3375 1854 1689 456
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 7134 7374
Size: 7134 240
FspI TGC'GCA
Cuts at: 0 4756 7374
Size: 4756 2618
FspAI rTGC'GCAy
Cuts at: 0 4756 7374
Size: 4756 2618
HaeII r_GCGC'y
Cuts at: 0 5498 7374
Size: 5498 1876
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 354 386 502 505 517 534 537 549
Size: 354 32 116 3 12 17 3 12
Cuts at: 549 927 959 1139 1171 1925 1957 4996 5028
Size: 378 32 180 32 754 32 3039 32
Cuts at: 5028 5064 5096 5660 5692 6013 6045 6515 6547
Size: 36 32 564 32 321 32 470 32
Cuts at: 6547 7374
Size: 827
Fragments arranged by size:
3039 827 754 564 470 378 354 321
180 116 36 32 32 32 32 32
32 32 32 32 17 12 12 3
3
HincII GTy'rAC
Cuts at: 0 2622 7374
Size: 2622 4752
HindIII A'AGCT_T
Cuts at: 0 1361 7374
Size: 1361 6013
KasI G'GCGC_C
Cuts at: 0 5494 7374
Size: 5494 1880
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 2246 3371 3575 4398 5228 5951 6827
Size: 548 1698 1125 204 823 830 723 876
Cuts at: 6827 7374
Size: 547
Fragments arranged by size:
1698 1125 876 830 823 723 548 547
204
MscI TGG'CCA
Cuts at: 0 1624 1663 2875 3532 7374
Size: 1624 39 1212 657 3842
Fragments arranged by size:
3842 1624 1212 657 39
MslI CAynn'nnrTG
Cuts at: 0 934 1777 1828 2152 2605 3220 3754 3955
Size: 934 843 51 324 453 615 534 201
Cuts at: 3955 4750 4843 5963 5983 6649 7374
Size: 795 93 1120 20 666 725
Fragments arranged by size:
1120 934 843 795 725 666 615 534
453 324 201 93 51 20
MspA1I CmG'CkG
Cuts at: 0 480 742 1572 1620 2416 3114 3288 3359
Size: 480 262 830 48 796 698 174 71
Cuts at: 3359 3368 3546 3633 4070 4497 5265 5937 6639
Size: 9 178 87 437 427 768 672 702
Cuts at: 6639 7040 7318 7374
Size: 401 278 56
Fragments arranged by size:
830 796 768 702 698 672 480 437
427 401 278 262 178 174 87 71
56 48 9
NaeI GCC'GGC
Cuts at: 0 1802 4861 6832 7374
Size: 1802 3059 1971 542
Fragments arranged by size:
3059 1971 1802 542
NarI GG'CG_CC
Cuts at: 0 5495 7374
Size: 5495 1879
NcoI C'CATG_G
Cuts at: 0 1823 1879 4103 4715 7352 7374
Size: 1823 56 2224 612 2637 22
Fragments arranged by size:
2637 2224 1823 612 56 22
NgoMIV G'CCGG_C
Cuts at: 0 1800 4859 6830 7374
Size: 1800 3059 1971 544
Fragments arranged by size:
3059 1971 1800 544
NruI TCG'CGA
Cuts at: 0 1556 6845 7374
Size: 1556 5289 529
Fragments arranged by size:
5289 1556 529
NsiI A_TGCA'T
Cuts at: 0 5960 7374
Size: 5960 1414
NspI r_CATG'y
Cuts at: 0 663 828 3492 3759 5532 6501 7374
Size: 663 165 2664 267 1773 969 873
Fragments arranged by size:
2664 1773 969 873 663 267 165
PasI CC'CwG_GG
Cuts at: 0 417 5133 6230 7374
Size: 417 4716 1097 1144
Fragments arranged by size:
4716 1144 1097 417
PciI A'CATG_T
Cuts at: 0 3488 3755 5528 6497 7374
Size: 3488 267 1773 969 877
Fragments arranged by size:
3488 1773 969 877 267
PflMI CCAn_nnn'nTGG
Cuts at: 0 1734 3501 4095 5133 5268 6876 7374
Size: 1734 1767 594 1038 135 1608 498
Fragments arranged by size:
1767 1734 1608 1038 594 498 135
PfoI T'CCnGG_A
Cuts at: 0 1996 2006 2219 2996 3260 3941 4706 5159
Size: 1996 10 213 777 264 681 765 453
Cuts at: 5159 6017 6947 7001 7374
Size: 858 930 54 373
Fragments arranged by size:
1996 930 858 777 765 681 453 373
264 213 54 10
PmlI CAC'GTG
Cuts at: 0 5538 7056 7374
Size: 5538 1518 318
Fragments arranged by size:
5538 1518 318
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4720 4752 6697 6729 6782 6814 7374
Size: 4720 32 1945 32 53 32 560
Fragments arranged by size:
4720 1945 560 53 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 906 1188 2406 5418 5901 6819 6849 7374
Size: 906 282 1218 3012 483 918 30 525
Fragments arranged by size:
3012 1218 918 906 525 483 282 30
PshAI GACnn'nnGTC
Cuts at: 0 637 7374
Size: 637 6737
PspOMI G'GGCC_C
Cuts at: 0 590 967 5053 7374
Size: 590 377 4086 2321
Fragments arranged by size:
4086 2321 590 377
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 7195 7227 7374
Size: 7195 32 147
Fragments arranged by size:
7195 147 32
PstI C_TGCA'G
Cuts at: 0 1250 3128 3287 3929 4685 6330 7034 7374
Size: 1250 1878 159 642 756 1645 704 340
Fragments arranged by size:
1878 1645 1250 756 704 642 340 159
PvuII CAG'CTG
Cuts at: 0 480 3546 3633 4497 5265 5937 7318 7374
Size: 480 3066 87 864 768 672 1381 56
Fragments arranged by size:
3066 1381 864 768 672 480 87 56
SacI G_AGCT'C
Cuts at: 0 1996 2888 3626 4058 6123 6596 7374
Size: 1996 892 738 432 2065 473 778
Fragments arranged by size:
2065 1996 892 778 738 473 432
SacII CC_GC'GG
Cuts at: 0 3369 7374
Size: 3369 4005
SapI GCTCTTCn'nnn_
Cuts at: 0 4063 4244 7374
Size: 4063 181 3130
Fragments arranged by size:
4063 3130 181
SbfI CC_TGCA'GG
Cuts at: 0 7034 7374
Size: 7034 340
SexAI A'CCwGG_T
Cuts at: 0 674 2978 3722 6506 6872 7010 7133 7374
Size: 674 2304 744 2784 366 138 123 241
Fragments arranged by size:
2784 2304 744 674 366 241 138 123
SfcI C'TryA_G
Cuts at: 0 1183 1246 1707 1813 2681 3124 3283 3925
Size: 1183 63 461 106 868 443 159 642
Cuts at: 3925 4681 4882 6326 7030 7374
Size: 756 201 1444 704 344
Fragments arranged by size:
1444 1183 868 756 704 642 461 443
344 201 159 106 63
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1138 7374
Size: 1138 6236
SfoI GGC'GCC
Cuts at: 0 5496 7374
Size: 5496 1878
SmaI CCC'GGG
Cuts at: 0 5452 7374
Size: 5452 1922
SmlI C'TyrA_G
Cuts at: 0 910 2476 3787 7234 7374
Size: 910 1566 1311 3447 140
Fragments arranged by size:
3447 1566 1311 910 140
SphI G_CATG'C
Cuts at: 0 828 7374
Size: 828 6546
SrfI GCCC'GGGC
Cuts at: 0 5452 7374
Size: 5452 1922
StuI AGG'CCT
Cuts at: 0 2072 6156 7114 7250 7374
Size: 2072 4084 958 136 124
Fragments arranged by size:
4084 2072 958 136 124
StyI C'CwwG_G
Cuts at: 0 1823 1879 3875 4103 4580 4715 5093 7352
Size: 1823 56 1996 228 477 135 378 2259
Cuts at: 7352 7374
Size: 22
Fragments arranged by size:
2259 1996 1823 477 378 228 135 56
22
SwaI ATTT'AAAT
Cuts at: 0 5577 7374
Size: 5577 1797
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 225 2242 3060 3337 4007 4561 5535 5929
Size: 225 2017 818 277 670 554 974 394
Cuts at: 5929 6090 6176 6600 6827 7374
Size: 161 86 424 227 547
Fragments arranged by size:
2017 974 818 670 554 547 424 394
277 227 225 161 86
TatI w'GTAC_w
Cuts at: 0 926 1437 2468 2720 2828 7374
Size: 926 511 1031 252 108 4546
Fragments arranged by size:
4546 1031 926 511 252 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1075 1107 2095 2127 2942 2974 3685 3717
Size: 1075 32 988 32 815 32 711 32
Cuts at: 3717 4860 4892 5285 5317 5345 5377 6956 6988
Size: 1143 32 393 32 28 32 1579 32
Cuts at: 6988 7374
Size: 386
Fragments arranged by size:
1579 1143 1075 988 815 711 393 386
32 32 32 32 32 32 32 32
28
Tth111I GACn'n_nGTC
Cuts at: 0 7012 7228 7374
Size: 7012 216 146
Fragments arranged by size:
7012 216 146
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 495 1199 1617 2145 2749 4722 4800 5475
Size: 495 704 418 528 604 1973 78 675
Cuts at: 5475 5934 7248 7374
Size: 459 1314 126
Fragments arranged by size:
1973 1314 704 675 604 528 495 459
418 126 78
XmaI C'CCGG_G
Cuts at: 0 5450 7374
Size: 5450 1924
ZraI GAC'GTC
Cuts at: 0 3825 7374
Size: 3825 3549
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflII AflIII AhdI
AleI AlfI AloI AlwNI ApaI ApoI AseI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NsiI NspI PasI PciI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PsrI PstI PvuII
SacI SacII SapI SbfI SexAI SfcI SfiI SfoI
SmaI SmlI SphI SrfI StuI StyI SwaI TaqII
TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AfeI AgeI ApaLI AscI AsiSI AvrII BmtI
BsiWI BsrGI BssHII BstZ17I DrdI FseI HpaI KpnI
MfeI MluI NdeI NheI NotI PacI PmeI PsiI
PspXI PvuI RsrII SalI SanDI ScaI SgfI SgrAI
SnaBI SpeI SspI XbaI XhoI XmnI