Restriction map for
pF1KB9017
(Linear) (Six-base) MAPSORT of: KIBB9017.seq Check: 4234 from: 1 to: 1329
KIBB9017.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AccI GT'mk_AC
Cuts at: 0 1074 1329
Size: 1074 255
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 628 1329
Size: 179 449 701
Fragments arranged by size:
701 449 179
AflII C'TTAA_G
Cuts at: 0 1230 1329
Size: 1230 99
AlwNI CAG_nnn'CTG
Cuts at: 0 747 1329
Size: 747 582
ApaLI G'TGCA_C
Cuts at: 0 306 1082 1329
Size: 306 776 247
Fragments arranged by size:
776 306 247
ApoI r'AATT_y
Cuts at: 0 532 802 826 1065 1329
Size: 532 270 24 239 264
Fragments arranged by size:
532 270 264 239 24
AseI AT'TA_AT
Cuts at: 0 688 712 1329
Size: 688 24 617
Fragments arranged by size:
688 617 24
AvaI C'yCGr_G
Cuts at: 0 140 485 512 1329
Size: 140 345 27 817
Fragments arranged by size:
817 345 140 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 709 742 1329
Size: 709 33 587
Fragments arranged by size:
709 587 33
BanII G_rGCy'C
Cuts at: 0 1148 1329
Size: 1148 181
BbsI GAAGACnn'nnnn_
Cuts at: 0 928 1329
Size: 928 401
BciVI GTATCCnnnnn_n'
Cuts at: 0 737 1306 1329
Size: 737 569 23
Fragments arranged by size:
737 569 23
BfrBI ATG'CAT
Cuts at: 0 820 1329
Size: 820 509
BglI GCCn_nnn'nGGC
Cuts at: 0 1112 1329
Size: 1112 217
Bme1580I G_kGCm'C
Cuts at: 0 310 1086 1329
Size: 310 776 243
Fragments arranged by size:
776 310 243
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 955 1193 1329
Size: 955 238 136
Fragments arranged by size:
955 238 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 579 901 1329
Size: 215 364 322 428
Fragments arranged by size:
428 364 322 215
BsaI GGTCTCn'nnnn_
Cuts at: 0 671 1329
Size: 671 658
BsaBI GATnn'nnATC
Cuts at: 0 629 1329
Size: 629 700
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 51 337 1329
Size: 51 286 992
Fragments arranged by size:
992 286 51
BseYI C'CCAG_C
Cuts at: 0 217 1329
Size: 217 1112
BsiHKAI G_wGCw'C
Cuts at: 0 310 1086 1148 1254 1329
Size: 310 776 62 106 75
Fragments arranged by size:
776 310 106 75 62
Bsp1286I G_dGCh'C
Cuts at: 0 310 1086 1148 1254 1329
Size: 310 776 62 106 75
Fragments arranged by size:
776 310 106 75 62
BsrDI GCAATG_nn'
Cuts at: 0 30 1329
Size: 30 1299
BsrFI r'CCGG_y
Cuts at: 0 1013 1329
Size: 1013 316
BstAPI GCAn_nnn'nTGC
Cuts at: 0 415 1329
Size: 415 914
BstBI TT'CG_AA
Cuts at: 0 1063 1329
Size: 1063 266
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1056 1329
Size: 1056 273
BtgI C'CryG_G
Cuts at: 0 681 1329
Size: 681 648
BtsI GCAGTG_nn'
Cuts at: 0 136 1009 1329
Size: 136 873 320
Fragments arranged by size:
873 320 136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 761 796 1329
Size: 761 35 533
Fragments arranged by size:
761 533 35
EaeI y'GGCC_r
Cuts at: 0 494 1329
Size: 494 835
EarI CTCTTCn'nnn_
Cuts at: 0 929 1329
Size: 929 400
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1000 1329
Size: 1000 329
EcoICRI GAG'CTC
Cuts at: 0 1146 1329
Size: 1146 183
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 179 628 955 1193 1329
Size: 179 449 327 238 136
Fragments arranged by size:
449 327 238 179 136
EcoNI CCTnn'n_nnAGG
Cuts at: 0 50 1329
Size: 50 1279
EcoO109I rG'GnC_Cy
Cuts at: 0 744 1329
Size: 744 585
EcoRI G'AATT_C
Cuts at: 0 1065 1329
Size: 1065 264
EcoRV GAT'ATC
Cuts at: 0 627 1329
Size: 627 702
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 958 1329
Size: 958 371
HaeII r_GCGC'y
Cuts at: 0 942 1329
Size: 942 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1241 1273 1329
Size: 1241 32 56
Fragments arranged by size:
1241 56 32
MslI CAynn'nnrTG
Cuts at: 0 179 1054 1329
Size: 179 875 275
Fragments arranged by size:
875 275 179
MspA1I CmG'CkG
Cuts at: 0 221 1329
Size: 221 1108
NaeI GCC'GGC
Cuts at: 0 1015 1329
Size: 1015 314
NcoI C'CATG_G
Cuts at: 0 681 1329
Size: 681 648
NgoMIV G'CCGG_C
Cuts at: 0 1013 1329
Size: 1013 316
NsiI A_TGCA'T
Cuts at: 0 822 1329
Size: 822 507
NspI r_CATG'y
Cuts at: 0 507 604 1329
Size: 507 97 725
Fragments arranged by size:
725 507 97
PflMI CCAn_nnn'nTGG
Cuts at: 0 822 1329
Size: 822 507
PfoI T'CCnGG_A
Cuts at: 0 740 980 1329
Size: 740 240 349
Fragments arranged by size:
740 349 240
PpuMI rG'GwC_Cy
Cuts at: 0 744 1329
Size: 744 585
PsiI TTA'TAA
Cuts at: 0 673 1329
Size: 673 656
PspXI vC'TCGA_Gb
Cuts at: 0 140 512 1329
Size: 140 372 817
Fragments arranged by size:
817 372 140
PvuII CAG'CTG
Cuts at: 0 221 1329
Size: 221 1108
SacI G_AGCT'C
Cuts at: 0 1148 1329
Size: 1148 181
SapI GCTCTTCn'nnn_
Cuts at: 0 929 1329
Size: 929 400
SfcI C'TryA_G
Cuts at: 0 443 1329
Size: 443 886
SmlI C'TyrA_G
Cuts at: 0 140 230 512 558 916 1230 1329
Size: 140 90 282 46 358 314 99
Fragments arranged by size:
358 314 282 140 99 90 46
StyI C'CwwG_G
Cuts at: 0 317 681 731 1329
Size: 317 364 50 598
Fragments arranged by size:
598 364 317 50
TatI w'GTAC_w
Cuts at: 0 152 1329
Size: 152 1177
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 414 446 1329
Size: 414 32 883
Fragments arranged by size:
883 414 32
XhoI C'TCGA_G
Cuts at: 0 140 512 1329
Size: 140 372 817
Fragments arranged by size:
817 372 140
XmnI GAAnn'nnTTC
Cuts at: 0 349 1329
Size: 349 980
Enzymes that do cut:
AccI AcuI AflII AlwNI ApaLI ApoI AseI AvaI
BaeI BanII BbsI BciVI BfrBI BglI Bme1580I BpmI
BpuEI BsaI BsaBI BseRI BseYI BsiHKAI Bsp1286I BsrDI
BsrFI BstAPI BstBI BstXI BtgI BtsI CspCI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PflMI PfoI PpuMI PsiI PspXI PvuII
SacI SapI SfcI SmlI StyI TatI TstI XhoI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AsiSI AvrII BamHI
BanI BbeI BbvCI BcgI BclI BglII BlpI BmgBI
BmrI BmtI BplI Bpu10I BsaAI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BssSI BstEII BstYI BstZ17I Bsu36I
BtgZI ClaI DraI DraIII DrdI EagI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NarI NdeI NheI NotI NruI PacI
PasI PciI PmeI PmlI PpiI PshAI PspOMI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI
XcmI XmaI ZraI