Restriction map for
pF1KB8999
(Linear) (Six-base) MAPSORT of: KIBB8999.seq Check: 366 from: 1 to: 1071
KIBB8999.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AflIII A'CryG_T
Cuts at: 0 818 1071
Size: 818 253
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 7 39 1071
Size: 7 32 1032
Fragments arranged by size:
1032 32 7
AlwNI CAG_nnn'CTG
Cuts at: 0 386 1071
Size: 386 685
ApaLI G'TGCA_C
Cuts at: 0 295 1071
Size: 295 776
BamHI G'GATC_C
Cuts at: 0 893 1071
Size: 893 178
BanI G'GyrC_C
Cuts at: 0 311 619 1071
Size: 311 308 452
Fragments arranged by size:
452 311 308
BanII G_rGCy'C
Cuts at: 0 697 917 1071
Size: 697 220 154
Fragments arranged by size:
697 220 154
BbeI G_GCGC'C
Cuts at: 0 315 623 1071
Size: 315 308 448
Fragments arranged by size:
448 315 308
BbsI GAAGACnn'nnnn_
Cuts at: 0 105 709 1071
Size: 105 604 362
Fragments arranged by size:
604 362 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 310 344 1071
Size: 310 34 727
Fragments arranged by size:
727 310 34
BglII A'GATC_T
Cuts at: 0 665 1071
Size: 665 406
BlpI GC'TnA_GC
Cuts at: 0 286 1071
Size: 286 785
Bme1580I G_kGCm'C
Cuts at: 0 224 299 501 1071
Size: 224 75 202 570
Fragments arranged by size:
570 224 202 75
BmgBI CAC'GTC
Cuts at: 0 363 1071
Size: 363 708
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 570 1071
Size: 570 501
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 681 1071
Size: 681 390
BsaI GGTCTCn'nnnn_
Cuts at: 0 49 1071
Size: 49 1022
BsaAI yAC'GTr
Cuts at: 0 821 1071
Size: 821 250
BsaHI Gr'CG_yC
Cuts at: 0 312 620 1071
Size: 312 308 451
Fragments arranged by size:
451 312 308
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 7 37 973 1003 1071
Size: 7 30 936 30 68
Fragments arranged by size:
936 68 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 583 1071
Size: 583 488
BseYI C'CCAG_C
Cuts at: 0 91 138 539 1071
Size: 91 47 401 532
Fragments arranged by size:
532 401 91 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 357 1071
Size: 357 714
BsiHKAI G_wGCw'C
Cuts at: 0 299 650 887 917 1071
Size: 299 351 237 30 154
Fragments arranged by size:
351 299 237 154 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 255 474 541 720 1071
Size: 255 219 67 179 351
Fragments arranged by size:
351 255 219 179 67
Bsp1286I G_dGCh'C
Cuts at: 0 224 299 501 650 697 887 917 1071
Size: 224 75 202 149 47 190 30 154
Fragments arranged by size:
224 202 190 154 149 75 47 30
BspHI T'CATG_A
Cuts at: 0 479 1071
Size: 479 592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 92 182 638 1071
Size: 92 90 456 433
Fragments arranged by size:
456 433 92 90
BsrBI CCG'CTC
Cuts at: 0 636 1071
Size: 636 435
BssSI C'ACGA_G
Cuts at: 0 298 1071
Size: 298 773
BstXI CCAn_nnnn'nTGG
Cuts at: 0 408 1071
Size: 408 663
BstYI r'GATC_y
Cuts at: 0 665 893 1071
Size: 665 228 178
Fragments arranged by size:
665 228 178
BtgI C'CryG_G
Cuts at: 0 242 1071
Size: 242 829
DraIII CAC_nnn'GTG
Cuts at: 0 219 1071
Size: 219 852
EaeI y'GGCC_r
Cuts at: 0 245 266 377 398 1071
Size: 245 21 111 21 673
Fragments arranged by size:
673 245 111 21 21
EarI CTCTTCn'nnn_
Cuts at: 0 655 1071
Size: 655 416
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 772 1071
Size: 772 299
EcoICRI GAG'CTC
Cuts at: 0 915 1071
Size: 915 156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 681 1071
Size: 681 390
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1071
Size: 1071
HaeII r_GCGC'y
Cuts at: 0 315 623 1043 1071
Size: 315 308 420 28
Fragments arranged by size:
420 315 308 28
KasI G'GCGC_C
Cuts at: 0 311 619 1071
Size: 311 308 452
Fragments arranged by size:
452 311 308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 497 1010 1071
Size: 310 187 513 61
Fragments arranged by size:
513 310 187 61
MslI CAynn'nnrTG
Cuts at: 0 874 1071
Size: 874 197
MspA1I CmG'CkG
Cuts at: 0 285 317 508 528 843 1071
Size: 285 32 191 20 315 228
Fragments arranged by size:
315 285 228 191 32 20
NarI GG'CG_CC
Cuts at: 0 312 620 1071
Size: 312 308 451
Fragments arranged by size:
451 312 308
NcoI C'CATG_G
Cuts at: 0 242 1071
Size: 242 829
PflMI CCAn_nnn'nTGG
Cuts at: 0 231 386 1071
Size: 231 155 685
Fragments arranged by size:
685 231 155
PfoI T'CCnGG_A
Cuts at: 0 697 1071
Size: 697 374
PmlI CAC'GTG
Cuts at: 0 821 1071
Size: 821 250
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 7 39 1071
Size: 7 32 1032
Fragments arranged by size:
1032 32 7
RsrII CG'GwC_CG
Cuts at: 0 780 1071
Size: 780 291
SacI G_AGCT'C
Cuts at: 0 917 1071
Size: 917 154
SapI GCTCTTCn'nnn_
Cuts at: 0 655 1071
Size: 655 416
SexAI A'CCwGG_T
Cuts at: 0 230 1071
Size: 230 841
SfoI GGC'GCC
Cuts at: 0 313 621 1071
Size: 313 308 450
Fragments arranged by size:
450 313 308
StyI C'CwwG_G
Cuts at: 0 242 1071
Size: 242 829
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 99 735 1071
Size: 99 636 336
Fragments arranged by size:
636 336 99
TatI w'GTAC_w
Cuts at: 0 656 1071
Size: 656 415
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 289 321 1071
Size: 289 32 750
Fragments arranged by size:
750 289 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 687 1071
Size: 687 384
Enzymes that do cut:
AflIII AloI AlwNI ApaLI BamHI BanI BanII BbeI
BbsI BcgI BglII BlpI Bme1580I BmgBI BplI BpmI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BssSI BstXI BstYI
BtgI DraIII EaeI EarI EciI EcoICRI Eco57MI FalI
HaeII KasI MmeI MslI MspA1I NarI NcoI PflMI
PfoI PmlI PpiI RsrII SacI SapI SexAI SfoI
StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI ApaI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BbvCI BciVI BclI BfrBI
BglI BmrI BmtI Bpu10I BpuEI BsaBI BsaWI BsiEI
BsiWI BsmI BspEI BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EagI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MscI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacII
SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI XmnI ZraI