Restriction map for
pF1KB8998
(Linear) (Six-base) MAPSORT of: KIBB8998.seq Check: 5681 from: 1 to: 1236
KIBB8998.orf
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 643 1236
Size: 261 382 593
Fragments arranged by size:
593 382 261
AlwNI CAG_nnn'CTG
Cuts at: 0 645 1236
Size: 645 591
AvaI C'yCGr_G
Cuts at: 0 962 1236
Size: 962 274
BanI G'GyrC_C
Cuts at: 0 561 952 1236
Size: 561 391 284
Fragments arranged by size:
561 391 284
BanII G_rGCy'C
Cuts at: 0 16 271 722 1154 1169 1190 1236
Size: 16 255 451 432 15 21 46
Fragments arranged by size:
451 432 255 46 21 16 15
BciVI GTATCCnnnnn_n'
Cuts at: 0 883 1236
Size: 883 353
Bme1580I G_kGCm'C
Cuts at: 0 566 663 963 1113 1183 1236
Size: 566 97 300 150 70 53
Fragments arranged by size:
566 300 150 97 70 53
BmgBI CAC'GTC
Cuts at: 0 918 1146 1236
Size: 918 228 90
Fragments arranged by size:
918 228 90
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 659 1236
Size: 659 577
Bpu10I CC'TnA_GC
Cuts at: 0 362 1061 1156 1236
Size: 362 699 95 80
Fragments arranged by size:
699 362 95 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1153 1236
Size: 1153 83
BsaWI w'CCGG_w
Cuts at: 0 329 1236
Size: 329 907
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 983 1013 1214 1236
Size: 983 30 201 22
Fragments arranged by size:
983 201 30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 6 1236
Size: 6 1230
BseYI C'CCAG_C
Cuts at: 0 83 383 579 1236
Size: 83 300 196 657
Fragments arranged by size:
657 300 196 83
BsiEI CG_ry'CG
Cuts at: 0 152 1236
Size: 152 1084
BsiHKAI G_wGCw'C
Cuts at: 0 173 271 1053 1154 1169 1236
Size: 173 98 782 101 15 67
Fragments arranged by size:
782 173 101 98 67 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1015 1236
Size: 1015 221
Bsp1286I G_dGCh'C
Cuts at: 0 16 173 271 566 663 722 963 1053
Size: 16 157 98 295 97 59 241 90
Cuts at: 1053 1113 1154 1169 1183 1190 1236
Size: 60 41 15 14 7 46
Fragments arranged by size:
295 241 157 98 97 90 60 59
46 41 16 15 14 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 116 987 1236
Size: 116 871 249
Fragments arranged by size:
871 249 116
BsrBI CCG'CTC
Cuts at: 0 327 657 1065 1236
Size: 327 330 408 171
Fragments arranged by size:
408 330 327 171
BsrDI GCAATG_nn'
Cuts at: 0 44 75 236 308 371 1236
Size: 44 31 161 72 63 865
Fragments arranged by size:
865 161 72 63 44 31
BsrFI r'CCGG_y
Cuts at: 0 349 1236
Size: 349 887
BsrGI T'GTAC_A
Cuts at: 0 23 1236
Size: 23 1213
BssSI C'ACGA_G
Cuts at: 0 354 1236
Size: 354 882
BstEII G'GTnAC_C
Cuts at: 0 345 1236
Size: 345 891
BstXI CCAn_nnnn'nTGG
Cuts at: 0 702 714 1034 1236
Size: 702 12 320 202
Fragments arranged by size:
702 320 202 12
BstYI r'GATC_y
Cuts at: 0 596 1236
Size: 596 640
Bsu36I CC'TnA_GG
Cuts at: 0 922 1236
Size: 922 314
BtgI C'CryG_G
Cuts at: 0 222 1236
Size: 222 1014
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 141 276 1236
Size: 141 135 960
Fragments arranged by size:
960 141 135
BtsI GCAGTG_nn'
Cuts at: 0 167 670 990 1053 1236
Size: 167 503 320 63 183
Fragments arranged by size:
503 320 183 167 63
EaeI y'GGCC_r
Cuts at: 0 41 56 149 287 704 815 1017 1236
Size: 41 15 93 138 417 111 202 219
Fragments arranged by size:
417 219 202 138 111 93 41 15
EagI C'GGCC_G
Cuts at: 0 149 1236
Size: 149 1087
EarI CTCTTCn'nnn_
Cuts at: 0 238 1236
Size: 238 998
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 874 1236
Size: 874 362
EcoICRI GAG'CTC
Cuts at: 0 269 1152 1167 1236
Size: 269 883 15 69
Fragments arranged by size:
883 269 69 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 643 1236
Size: 261 382 593
Fragments arranged by size:
593 382 261
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1213 1236
Size: 1213 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 983 1015 1236
Size: 983 32 221
Fragments arranged by size:
983 221 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 1236
Size: 406 830
MscI TGG'CCA
Cuts at: 0 43 58 289 706 817 1236
Size: 43 15 231 417 111 419
Fragments arranged by size:
419 417 231 111 43 15
MslI CAynn'nnrTG
Cuts at: 0 31 49 160 712 983 1236
Size: 31 18 111 552 271 253
Fragments arranged by size:
552 271 253 111 31 18
MspA1I CmG'CkG
Cuts at: 0 621 949 1236
Size: 621 328 287
Fragments arranged by size:
621 328 287
PasI CC'CwG_GG
Cuts at: 0 468 1086 1122 1236
Size: 468 618 36 114
Fragments arranged by size:
618 468 114 36
PflMI CCAn_nnn'nTGG
Cuts at: 0 383 1236
Size: 383 853
PstI C_TGCA'G
Cuts at: 0 113 677 787 1236
Size: 113 564 110 449
Fragments arranged by size:
564 449 113 110
PvuII CAG'CTG
Cuts at: 0 621 949 1236
Size: 621 328 287
Fragments arranged by size:
621 328 287
SacI G_AGCT'C
Cuts at: 0 271 1154 1169 1236
Size: 271 883 15 67
Fragments arranged by size:
883 271 67 15
SfcI C'TryA_G
Cuts at: 0 109 673 783 1236
Size: 109 564 110 453
Fragments arranged by size:
564 453 110 109
SmaI CCC'GGG
Cuts at: 0 964 1236
Size: 964 272
SmlI C'TyrA_G
Cuts at: 0 1168 1236
Size: 1168 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 154 375 571 1236
Size: 154 221 196 665
Fragments arranged by size:
665 221 196 154
TatI w'GTAC_w
Cuts at: 0 23 533 1236
Size: 23 510 703
Fragments arranged by size:
703 510 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1214 1236
Size: 1214 22
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 51 129 525 1236
Size: 51 78 396 711
Fragments arranged by size:
711 396 78 51
XmaI C'CCGG_G
Cuts at: 0 962 1236
Size: 962 274
XmnI GAAnn'nnTTC
Cuts at: 0 853 1236
Size: 853 383
Enzymes that do cut:
AcuI AlwNI AvaI BanI BanII BciVI Bme1580I BmgBI
BplI Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI Hin4I
MmeI MscI MslI MspA1I PasI PflMI PstI PvuII
SacI SfcI SmaI SmlI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI BmrI
BmtI BpmI BsaI BsaAI BsaBI BsaHI BsgI BsiWI
BsmI BspEI BspHI BssHII BstAPI BstBI BstZ17I ClaI
CspCI DraI DraIII DrdI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI Tth111I XbaI
XhoI ZraI