Restriction map for
pF1KB8994
(Linear) (Six-base) MAPSORT of: KIBB8994.seq Check: 7480 from: 1 to: 987
KIBB8994.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 153 987
Size: 153 834
AccI GT'mk_AC
Cuts at: 0 260 971 987
Size: 260 711 16
Fragments arranged by size:
711 260 16
AfeI AGC'GCT
Cuts at: 0 325 853 987
Size: 325 528 134
Fragments arranged by size:
528 325 134
AlwNI CAG_nnn'CTG
Cuts at: 0 900 987
Size: 900 87
ApaI G_GGCC'C
Cuts at: 0 648 713 987
Size: 648 65 274
Fragments arranged by size:
648 274 65
ApaLI G'TGCA_C
Cuts at: 0 313 987
Size: 313 674
AvaI C'yCGr_G
Cuts at: 0 641 917 987
Size: 641 276 70
Fragments arranged by size:
641 276 70
AvrII C'CTAG_G
Cuts at: 0 798 987
Size: 798 189
BanI G'GyrC_C
Cuts at: 0 24 451 887 987
Size: 24 427 436 100
Fragments arranged by size:
436 427 100 24
BanII G_rGCy'C
Cuts at: 0 59 515 629 648 713 987
Size: 59 456 114 19 65 274
Fragments arranged by size:
456 274 114 65 59 19
BglI GCCn_nnn'nGGC
Cuts at: 0 768 987
Size: 768 219
Bme1580I G_kGCm'C
Cuts at: 0 29 317 648 672 713 827 987
Size: 29 288 331 24 41 114 160
Fragments arranged by size:
331 288 160 114 41 29 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 775 987
Size: 775 212
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 622 675 698 888 987
Size: 334 288 53 23 190 99
Fragments arranged by size:
334 288 190 99 53 23
Bpu10I CC'TnA_GC
Cuts at: 0 163 987
Size: 163 824
BsaAI yAC'GTr
Cuts at: 0 717 987
Size: 717 270
BsaHI Gr'CG_yC
Cuts at: 0 4 987
Size: 4 983
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 448 478 987
Size: 448 30 509
Fragments arranged by size:
509 448 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 244 373 721 781 987
Size: 244 129 348 60 206
Fragments arranged by size:
348 244 206 129 60
BseYI C'CCAG_C
Cuts at: 0 28 178 480 704 840 934 987
Size: 28 150 302 224 136 94 53
Fragments arranged by size:
302 224 150 136 94 53 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 833 987
Size: 833 154
BsiHKAI G_wGCw'C
Cuts at: 0 317 987
Size: 317 670
BsmI GAATG_Cn'
Cuts at: 0 365 987
Size: 365 622
Bsp1286I G_dGCh'C
Cuts at: 0 29 59 317 515 629 648 672 713
Size: 29 30 258 198 114 19 24 41
Cuts at: 713 827 987
Size: 114 160
Fragments arranged by size:
258 198 160 114 114 41 30 29
24 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 104 153 987
Size: 104 49 834
Fragments arranged by size:
834 104 49
BsrBI CCG'CTC
Cuts at: 0 424 606 987
Size: 424 182 381
Fragments arranged by size:
424 381 182
BsrDI GCAATG_nn'
Cuts at: 0 199 205 938 987
Size: 199 6 733 49
Fragments arranged by size:
733 199 49 6
BsrFI r'CCGG_y
Cuts at: 0 596 987
Size: 596 391
BstXI CCAn_nnnn'nTGG
Cuts at: 0 218 358 840 987
Size: 218 140 482 147
Fragments arranged by size:
482 218 147 140
Bsu36I CC'TnA_GG
Cuts at: 0 340 987
Size: 340 647
BtgI C'CryG_G
Cuts at: 0 527 833 987
Size: 527 306 154
Fragments arranged by size:
527 306 154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 99 635 987
Size: 99 536 352
Fragments arranged by size:
536 352 99
BtsI GCAGTG_nn'
Cuts at: 0 566 617 724 827 972 987
Size: 566 51 107 103 145 15
Fragments arranged by size:
566 145 107 103 51 15
DrdI GACnn_nn'nnGTC
Cuts at: 0 783 987
Size: 783 204
EaeI y'GGCC_r
Cuts at: 0 563 719 987
Size: 563 156 268
Fragments arranged by size:
563 268 156
EarI CTCTTCn'nnn_
Cuts at: 0 697 903 987
Size: 697 206 84
Fragments arranged by size:
697 206 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 621 987
Size: 621 366
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 622 675 698 888 987
Size: 334 288 53 23 190 99
Fragments arranged by size:
334 288 190 99 53 23
EcoO109I rG'GnC_Cy
Cuts at: 0 645 709 710 769 987
Size: 645 64 1 59 218
Fragments arranged by size:
645 218 64 59 1
FspI TGC'GCA
Cuts at: 0 136 987
Size: 136 851
HaeII r_GCGC'y
Cuts at: 0 327 759 855 987
Size: 327 432 96 132
Fragments arranged by size:
432 327 132 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 613 645 987
Size: 613 32 342
Fragments arranged by size:
613 342 32
HincII GTy'rAC
Cuts at: 0 972 987
Size: 972 15
MscI TGG'CCA
Cuts at: 0 565 721 987
Size: 565 156 266
Fragments arranged by size:
565 266 156
MspA1I CmG'CkG
Cuts at: 0 582 817 987
Size: 582 235 170
Fragments arranged by size:
582 235 170
NaeI GCC'GGC
Cuts at: 0 598 987
Size: 598 389
NcoI C'CATG_G
Cuts at: 0 833 987
Size: 833 154
NgoMIV G'CCGG_C
Cuts at: 0 596 987
Size: 596 391
PasI CC'CwG_GG
Cuts at: 0 108 358 987
Size: 108 250 629
Fragments arranged by size:
629 250 108
PflMI CCAn_nnn'nTGG
Cuts at: 0 148 987
Size: 148 839
PspOMI G'GGCC_C
Cuts at: 0 644 709 987
Size: 644 65 278
Fragments arranged by size:
644 278 65
PstI C_TGCA'G
Cuts at: 0 463 587 896 987
Size: 463 124 309 91
Fragments arranged by size:
463 309 124 91
PvuII CAG'CTG
Cuts at: 0 582 987
Size: 582 405
SalI G'TCGA_C
Cuts at: 0 970 987
Size: 970 17
SapI GCTCTTCn'nnn_
Cuts at: 0 697 903 987
Size: 697 206 84
Fragments arranged by size:
697 206 84
SbfI CC_TGCA'GG
Cuts at: 0 896 987
Size: 896 91
SfcI C'TryA_G
Cuts at: 0 459 583 892 987
Size: 459 124 309 95
Fragments arranged by size:
459 309 124 95
SmaI CCC'GGG
Cuts at: 0 643 919 987
Size: 643 276 68
Fragments arranged by size:
643 276 68
SrfI GCCC'GGGC
Cuts at: 0 643 987
Size: 643 344
StyI C'CwwG_G
Cuts at: 0 41 798 833 987
Size: 41 757 35 154
Fragments arranged by size:
757 154 41 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 520 965 987
Size: 520 445 22
Fragments arranged by size:
520 445 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 435 467 987
Size: 435 32 520
Fragments arranged by size:
520 435 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 48 306 445 987
Size: 48 258 139 542
Fragments arranged by size:
542 258 139 48
XmaI C'CCGG_G
Cuts at: 0 641 917 987
Size: 641 276 70
Fragments arranged by size:
641 276 70
Enzymes that do cut:
AarI AccI AfeI AlwNI ApaI ApaLI AvaI AvrII
BanI BanII BglI Bme1580I BmrI BpmI Bpu10I BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrDI BsrFI BstXI Bsu36I BtgI BtgZI
BtsI DrdI EaeI EarI EciI Eco57MI EcoO109I FspI
HaeII Hin4I HincII MscI MspA1I NaeI NcoI NgoMIV
PasI PflMI PspOMI PstI PvuII SalI SapI SbfI
SfcI SmaI SrfI StyI TaqII TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AflII AflIII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaBI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I ClaI CspCI
DraI DraIII EagI EcoICRI EcoNI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
MmeI MslI NarI NdeI NheI NotI NruI NsiI
NspI PacI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SspI StuI SwaI TatI Tth111I
XbaI XhoI XmnI ZraI