Restriction map for
pF1KB8993
(Linear) (Six-base) MAPSORT of: KIBB8993.seq Check: 1617 from: 1 to: 1203
KIBB8993.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 713 1203
Size: 41 672 490
Fragments arranged by size:
672 490 41
AflII C'TTAA_G
Cuts at: 0 504 1203
Size: 504 699
AflIII A'CryG_T
Cuts at: 0 6 1203
Size: 6 1197
AhdI GACnn_n'nnGTC
Cuts at: 0 117 1203
Size: 117 1086
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 80 112 1203
Size: 80 32 1091
Fragments arranged by size:
1091 80 32
ApoI r'AATT_y
Cuts at: 0 344 434 444 534 729 780 945 1203
Size: 344 90 10 90 195 51 165 258
Fragments arranged by size:
344 258 195 165 90 90 51 10
AseI AT'TA_AT
Cuts at: 0 320 1203
Size: 320 883
BamHI G'GATC_C
Cuts at: 0 160 1203
Size: 160 1043
BglII A'GATC_T
Cuts at: 0 454 1203
Size: 454 749
BmtI G_CTAG'C
Cuts at: 0 1159 1203
Size: 1159 44
Bpu10I CC'TnA_GC
Cuts at: 0 684 1203
Size: 684 519
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 1203
Size: 8 1195
BsaI GGTCTCn'nnnn_
Cuts at: 0 80 1203
Size: 80 1123
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 60 90 345 375 1203
Size: 60 30 255 30 828
Fragments arranged by size:
828 255 60 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 803 1203
Size: 803 400
BsiEI CG_ry'CG
Cuts at: 0 1124 1203
Size: 1124 79
BstYI r'GATC_y
Cuts at: 0 160 454 1203
Size: 160 294 749
Fragments arranged by size:
749 294 160
BtgI C'CryG_G
Cuts at: 0 1102 1203
Size: 1102 101
DraI TTT'AAA
Cuts at: 0 734 878 1203
Size: 734 144 325
Fragments arranged by size:
734 325 144
DraIII CAC_nnn'GTG
Cuts at: 0 942 1203
Size: 942 261
EarI CTCTTCn'nnn_
Cuts at: 0 337 479 568 1166 1203
Size: 337 142 89 598 37
Fragments arranged by size:
598 337 142 89 37
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 713 1203
Size: 41 672 490
Fragments arranged by size:
672 490 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 69 1203
Size: 69 1134
EcoRV GAT'ATC
Cuts at: 0 1045 1203
Size: 1045 158
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 773 1203
Size: 773 430
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 135 478 510 1203
Size: 103 32 343 32 693
Fragments arranged by size:
693 343 103 32 32
HincII GTy'rAC
Cuts at: 0 887 1203
Size: 887 316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 816 1203
Size: 816 387
MspA1I CmG'CkG
Cuts at: 0 1138 1203
Size: 1138 65
NdeI CA'TA_TG
Cuts at: 0 899 1203
Size: 899 304
NheI G'CTAG_C
Cuts at: 0 1155 1203
Size: 1155 48
NspI r_CATG'y
Cuts at: 0 10 139 871 924 1086 1203
Size: 10 129 732 53 162 117
Fragments arranged by size:
732 162 129 117 53 10
PasI CC'CwG_GG
Cuts at: 0 100 1203
Size: 100 1103
PciI A'CATG_T
Cuts at: 0 6 1203
Size: 6 1197
PsiI TTA'TAA
Cuts at: 0 527 1203
Size: 527 676
PvuI CG_AT'CG
Cuts at: 0 1124 1203
Size: 1124 79
PvuII CAG'CTG
Cuts at: 0 1138 1203
Size: 1138 65
SapI GCTCTTCn'nnn_
Cuts at: 0 1166 1203
Size: 1166 37
SmlI C'TyrA_G
Cuts at: 0 23 504 1203
Size: 23 481 699
Fragments arranged by size:
699 481 23
SspI AAT'ATT
Cuts at: 0 374 1203
Size: 374 829
SwaI ATTT'AAAT
Cuts at: 0 734 1203
Size: 734 469
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 60 92 1029 1061 1203
Size: 60 32 937 32 142
Fragments arranged by size:
937 142 60 32 32
Enzymes that do cut:
AcuI AflII AflIII AhdI AloI ApoI AseI BamHI
BglII BmtI Bpu10I BpuEI BsaI BsaXI BseRI BsiEI
BstYI BtgI DraI DraIII EarI Eco57MI EcoNI EcoRV
FalI Hin4I HincII MmeI MspA1I NdeI NheI NspI
PasI PciI PsiI PvuI PvuII SapI SmlI SspI
SwaI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AleI
AlfI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DrdI
EaeI EagI EciI EcoICRI EcoO109I EcoRI FseI FspI
FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NcoI NgoMIV NotI NruI
NsiI PacI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
StyI TaqII TatI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI