Restriction map for
pF1KB8989
(Linear) (Six-base) MAPSORT of: KIBB8989.seq Check: 1178 from: 1 to: 1170
KIBB8989.orf
With 182 enzymes: SgfI *
March 3, 2008 11:09 ..
AatII G_ACGT'C
Cuts at: 0 877 1170
Size: 877 293
AccI GT'mk_AC
Cuts at: 0 194 944 1170
Size: 194 750 226
Fragments arranged by size:
750 226 194
Acc65I G'GTAC_C
Cuts at: 0 44 1170
Size: 44 1126
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1170
Size: 889 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 691 1170
Size: 691 479
ApaLI G'TGCA_C
Cuts at: 0 1105 1170
Size: 1105 65
AvaI C'yCGr_G
Cuts at: 0 40 574 1170
Size: 40 534 596
Fragments arranged by size:
596 534 40
BanI G'GyrC_C
Cuts at: 0 44 440 843 951 1033 1170
Size: 44 396 403 108 82 137
Fragments arranged by size:
403 396 137 108 82 44
BanII G_rGCy'C
Cuts at: 0 87 1170
Size: 87 1083
BbeI G_GCGC'C
Cuts at: 0 444 1037 1170
Size: 444 593 133
Fragments arranged by size:
593 444 133
BbsI GAAGACnn'nnnn_
Cuts at: 0 370 733 766 799 877 1170
Size: 370 363 33 33 78 293
Fragments arranged by size:
370 363 293 78 33 33
BclI T'GATC_A
Cuts at: 0 128 1170
Size: 128 1042
BlpI GC'TnA_GC
Cuts at: 0 163 277 1170
Size: 163 114 893
Fragments arranged by size:
893 163 114
Bme1580I G_kGCm'C
Cuts at: 0 835 848 1109 1170
Size: 835 13 261 61
Fragments arranged by size:
835 261 61 13
BmgBI CAC'GTC
Cuts at: 0 972 1104 1170
Size: 972 132 66
Fragments arranged by size:
972 132 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 90 1170
Size: 90 1080
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 352 403 433 616 775 808 829
Size: 244 108 51 30 183 159 33 21
Cuts at: 829 1138 1170
Size: 309 32
Fragments arranged by size:
309 244 183 159 108 51 33 32
30 21
Bpu10I CC'TnA_GC
Cuts at: 0 86 1170
Size: 86 1084
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 197 754 991 1170
Size: 197 557 237 179
Fragments arranged by size:
557 237 197 179
BsaAI yAC'GTr
Cuts at: 0 915 1170
Size: 915 255
BsaHI Gr'CG_yC
Cuts at: 0 53 441 874 1034 1139 1170
Size: 53 388 433 160 105 31
Fragments arranged by size:
433 388 160 105 53 31
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 88 118 589 619 913 943 1170
Size: 88 30 471 30 294 30 227
Fragments arranged by size:
471 294 227 88 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 241 565 592 643 655 1144 1170
Size: 112 129 324 27 51 12 489 26
Fragments arranged by size:
489 324 129 112 51 27 26 12
BseYI C'CCAG_C
Cuts at: 0 79 285 555 828 1170
Size: 79 206 270 273 342
Fragments arranged by size:
342 273 270 206 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1170
Size: 277 893
BsiEI CG_ry'CG
Cuts at: 0 34 1170
Size: 34 1136
BsiHKAI G_wGCw'C
Cuts at: 0 1007 1109 1170
Size: 1007 102 61
Fragments arranged by size:
1007 102 61
Bsp1286I G_dGCh'C
Cuts at: 0 87 835 848 1007 1109 1170
Size: 87 748 13 159 102 61
Fragments arranged by size:
748 159 102 87 61 13
BspHI T'CATG_A
Cuts at: 0 458 1170
Size: 458 712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1088 1170
Size: 1088 82
BsrBI CCG'CTC
Cuts at: 0 30 231 570 855 1170
Size: 30 201 339 285 315
Fragments arranged by size:
339 315 285 201 30
BsrFI r'CCGG_y
Cuts at: 0 112 486 1072 1170
Size: 112 374 586 98
Fragments arranged by size:
586 374 112 98
BssHII G'CGCG_C
Cuts at: 0 925 1170
Size: 925 245
BssSI C'ACGA_G
Cuts at: 0 306 1170
Size: 306 864
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1113 1170
Size: 1113 57
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1020 1170
Size: 1020 150
BstYI r'GATC_y
Cuts at: 0 200 980 1170
Size: 200 780 190
Fragments arranged by size:
780 200 190
BtgI C'CryG_G
Cuts at: 0 938 1170
Size: 938 232
BtsI GCAGTG_nn'
Cuts at: 0 260 1170
Size: 260 910
DraI TTT'AAA
Cuts at: 0 453 1170
Size: 453 717
DraIII CAC_nnn'GTG
Cuts at: 0 918 1170
Size: 918 252
EaeI y'GGCC_r
Cuts at: 0 31 488 1170
Size: 31 457 682
Fragments arranged by size:
682 457 31
EagI C'GGCC_G
Cuts at: 0 31 1170
Size: 31 1139
EarI CTCTTCn'nnn_
Cuts at: 0 116 1170
Size: 116 1054
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 253 1170
Size: 253 917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 352 403 433 616 775 808 829
Size: 244 108 51 30 183 159 33 21
Cuts at: 829 889 1138 1170
Size: 60 249 32
Fragments arranged by size:
249 244 183 159 108 60 51 33
32 30 21
EcoO109I rG'GnC_Cy
Cuts at: 0 6 60 1170
Size: 6 54 1110
Fragments arranged by size:
1110 54 6
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 720 1170
Size: 720 450
FspI TGC'GCA
Cuts at: 0 343 892 1170
Size: 343 549 278
Fragments arranged by size:
549 343 278
HaeII r_GCGC'y
Cuts at: 0 390 444 532 595 1037 1170
Size: 390 54 88 63 442 133
Fragments arranged by size:
442 390 133 88 63 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 589 621 1170
Size: 88 32 469 32 549
Fragments arranged by size:
549 469 88 32 32
HincII GTy'rAC
Cuts at: 0 357 420 1170
Size: 357 63 750
Fragments arranged by size:
750 357 63
KasI G'GCGC_C
Cuts at: 0 440 1033 1170
Size: 440 593 137
Fragments arranged by size:
593 440 137
KpnI G_GTAC'C
Cuts at: 0 48 1170
Size: 48 1122
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 116 503 587 1115 1170
Size: 116 387 84 528 55
Fragments arranged by size:
528 387 116 84 55
MslI CAynn'nnrTG
Cuts at: 0 1018 1170
Size: 1018 152
MspA1I CmG'CkG
Cuts at: 0 79 94 207 222 589 671 940 1170
Size: 79 15 113 15 367 82 269 230
Fragments arranged by size:
367 269 230 113 82 79 15 15
NaeI GCC'GGC
Cuts at: 0 114 488 1074 1170
Size: 114 374 586 96
Fragments arranged by size:
586 374 114 96
NarI GG'CG_CC
Cuts at: 0 441 1034 1170
Size: 441 593 136
Fragments arranged by size:
593 441 136
NgoMIV G'CCGG_C
Cuts at: 0 112 486 1072 1170
Size: 112 374 586 98
Fragments arranged by size:
586 374 112 98
NotI GC'GGCC_GC
Cuts at: 0 31 1170
Size: 31 1139
PfoI T'CCnGG_A
Cuts at: 0 170 1170
Size: 170 1000
PmlI CAC'GTG
Cuts at: 0 915 1170
Size: 915 255
PpuMI rG'GwC_Cy
Cuts at: 0 6 1170
Size: 6 1164
PshAI GACnn'nnGTC
Cuts at: 0 940 1170
Size: 940 230
PvuII CAG'CTG
Cuts at: 0 207 222 1170
Size: 207 15 948
Fragments arranged by size:
948 207 15
SacII CC_GC'GG
Cuts at: 0 941 1170
Size: 941 229
SapI GCTCTTCn'nnn_
Cuts at: 0 116 1170
Size: 116 1054
SexAI A'CCwGG_T
Cuts at: 0 140 1170
Size: 140 1030
SfoI GGC'GCC
Cuts at: 0 442 1035 1170
Size: 442 593 135
Fragments arranged by size:
593 442 135
SmaI CCC'GGG
Cuts at: 0 42 1170
Size: 42 1128
SmlI C'TyrA_G
Cuts at: 0 212 769 1006 1170
Size: 212 557 237 164
Fragments arranged by size:
557 237 212 164
StuI AGG'CCT
Cuts at: 0 703 1170
Size: 703 467
StyI C'CwwG_G
Cuts at: 0 710 731 776 1170
Size: 710 21 45 394
Fragments arranged by size:
710 394 45 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 717 783 1020 1170
Size: 717 66 237 150
Fragments arranged by size:
717 237 150 66
XmaI C'CCGG_G
Cuts at: 0 40 1170
Size: 40 1130
XmnI GAAnn'nnTTC
Cuts at: 0 703 1170
Size: 703 467
ZraI GAC'GTC
Cuts at: 0 875 1170
Size: 875 295
Enzymes that do cut:
AatII AccI Acc65I AcuI AlfI ApaLI AvaI BanI
BanII BbeI BbsI BclI BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI
BssHII BssSI BstAPI BstXI BstYI BtgI BtsI DraI
DraIII EaeI EagI EarI EciI Eco57MI EcoO109I FalI
FspI HaeII Hin4I HincII KasI KpnI MmeI MslI
MspA1I NaeI NarI NgoMIV NotI PfoI PmlI PpuMI
PshAI PvuII SacII SapI SexAI SfoI SmaI SmlI
StuI StyI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AhdI AleI
AloI AlwNI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BfrBI BglI BglII
BmrI BmtI BsaI BsaBI BsaWI BsiWI BsmI BsmBI
BspEI BsrDI BsrGI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DrdI EcoICRI EcoNI EcoRI EcoRV FseI
FspAI HindIII HpaI MfeI MluI MscI NcoI NdeI
NheI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PpiI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SalI SanDI SbfI ScaI SfcI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI TstI Tth111I XbaI XhoI